j**********5
发帖数: 11 | 1 求审稿机会，为了准备申请绿卡。。Phd期间帮助老板审过七八次。。但
是老板坚持说编辑喜欢让教授审稿或者让以教授的名义让学生审稿，没有跟我推荐任何
的机会。。 发了十几个杂志， 快半年了就只拿到一个审稿机会，剩下的都回复说加了
list就再也没有下文了。。希望大家有
类似方向的推荐一下。。如果需要CV 我可以私下发过去。。大谢！！！
I received my PhD degree in Chemical Engineering at UT-Austin. I have 6
years research
experience in colloids and interface science. Specifically my expertise are
nanoparticle synthesis, colloidal stability, polymer coating/composites,
adsorption, worm-like micelles, emulsions/foams stability, rheology,
Pickering emulsions.
Thanks!... 阅读全帖 |
|
发帖数: 1 | 2 化学/高分子/材料 求推荐审稿机会
才完成PhD,准备开始申请绿卡,在这里向各位大牛求审稿机会
涉及的领域主要有polymer/macromoleculear chemistry, self-assembly, drug
delivery, organic electronic materials, hybrid materials, block copolymer,
stimuli-responsive polymer, micelles and hydrogels等
email:[email protected]
如需要更多信息（比如CV或者publication list）请email我，多谢多谢 |
|
ET
发帖数: 10701 | 3 一直在犹豫着是不是该将这段经历-学习Golf过程中的一些感悟-写下来. 对了, 还有这段
日子,也许很可能是我这几年来最幸福一段日子.
这里最主要的原因在于,我不能分辨我所写的,或者说,你可能读到的,究竟能make多少sens
e对于并不打Golf或甚至从来不看Golf比赛的你.
于是,告诉自己:如果想象不出谁会是读者,那就当为自己而写吧.
一段经历,一个成长的片段.
又:下午NBC转播Laureus World Sport Award, Newcomer Award授予了身高6', 年仅14岁
的韩国裔美国Golf运动员, Micelle Wei. 她击败了如日中天的竞争者James. see how
amazing the sport is. |
|
ET
发帖数: 10701 | 4 【 以下文字转载自 ZJU 讨论区,原文如下 】
发信人: ET (), 信区: ZJU
标 题: Golf备忘录-前言
发信站: Unknown Space - 未名空间 (Mon May 24 00:05:23 2004) WWW-POST
一直在犹豫着是不是该将这段经历-学习Golf过程中的一些感悟-写下来. 对了, 还有这段
日子,也许很可能是我这几年来最幸福一段日子.
这里最主要的原因在于,我不能分辨我所写的,或者说,你可能读到的,究竟能make多少sens
e对于并不打Golf或甚至从来不看Golf比赛的你.
于是,告诉自己:如果想象不出谁会是读者,那就当为自己而写吧.
一段经历,一个成长的片段.
又:下午NBC转播Laureus World Sport Award, Newcomer Award授予了身高6', 年仅14岁
的韩国裔美国Golf运动员, Micelle Wei. 她击败了如日中天的竞争者James. see how
amazing the sport is.
E.T. |
|
f****6
发帖数: 4472 | 5 我现在就悲催得做一个micelle formulation的小课题，各种闹心 |
|
v*****s
发帖数: 20290 | 6 这个很难。目前为止，我所知道的唯一方法就是用micelle把水分子一个个裹起来才有
可能。 |
|
|
s****r
发帖数: 342 | 8 we went to a chinese resturant tonight, but had not very good experience.
no chinese menu, no chinese waiters, only one chinese cook ( we were told)
who did not work today. The food, of course, was not the one we expected,
almost all of them are fried. I had to ask a vegatable finally, it was
actually a micellous sterilized by spoiled water. faint.. |
|
ET
发帖数: 10701 | 9 一直在犹豫着是不是该将这段经历-学习Golf过程中的一些感悟-写下来. 对了, 还有这段
日子,也许很可能是我这几年来最幸福一段日子.
这里最主要的原因在于,我不能分辨我所写的,或者说,你可能读到的,究竟能make多少sens
e对于并不打Golf或甚至从来不看Golf比赛的你.
于是,告诉自己:如果想象不出谁会是读者,那就当为自己而写吧.
一段经历,一个成长的片段.
又:下午NBC转播Laureus World Sport Award, Newcomer Award授予了身高6', 年仅14岁
的韩国裔美国Golf运动员, Micelle Wei. 她击败了如日中天的竞争者James. see how
amazing the sport is.
E.T. |
|
w*s
发帖数: 7227 | 10 we're using Micrel phy chip,
in linux_src/.../drivers/net/phy,
the micrel.c is there, thank God it's the correct chip version for me.
Now i need to make a MAC driver for it.
Where should i get started ? any samples ?
Also why they just put the PHY code there, not the MAC ?
How ppl can use it ?
I hope MAC drivers are just using some generic PHY devices,
then PHY is mapped to Micel in my case.
Any help appreciated. |
|
c********b
发帖数: 363 | 11 我没有试过,不知道.你可以自己试一试.
理论上Triton X-100可以把蛋白上associate的SDS洗脱下来形成micelles,然后蛋白可
以得以复性.至于这样对sepharose柱子有什么影响我就不知道了.但是可以试试.
good luck |
|
s******y
发帖数: 28562 | 12 我猜他的意思是有些蛋白提纯的时候会有detergent 带进来，而detergent 本身
因为会形成那种小团状micelle（我为了写那个字都老羞成怒了，不小心就会
被改成michelle），所以个头不小。如果那个蛋白本身也很小的话，用超滤膜就
会出现不能把蛋白和detergent分开的现象。 |
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k******0
发帖数: 1073 | 13 detergent micelle挺大的，用30kDa MWCO都很难透析掉。 |
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b******y
发帖数: 627 | 14 My guess is as following:
he/she is purifying membrane proteins (or protein complexes). Proper
interaction with detergent is required for its solubility and homogenous
behavior. Otherwise, it is death penalty for crystallization.
If using centricon or amicon, the protein sample will be overly concentrated
at the bottom of the tubes, so will be the detergent. In this case, it
might be over its critical concentration and therefore is likely to form
micelle (I don't have problem with michelle!). At... 阅读全帖 |
|
k******0
发帖数: 1073 | 15 your guess is mostly right.
In my case, because detergent micelle (my detergent has a low CMC) cannot
pass through the concentration membrane and therefore will get concentrated
as well as the protein complex. Subunit interaction will get disrupted and
the protein complex will be broken apart by the high concentration of
detergent.
concentrated
sample |
|
b**y
发帖数: 86 | 16 个人感觉我们对于生物起源的了解实在是太少太少，离我们能够确定的说“生命就是这
样开始的”程度还差的太远太远。作者所说的最多只是一个假说，而作者的很多所谓的
论据都是断章取义，有些故弄玄虚，其实并没有多少说服力：
比如说所谓的“斯皮格曼的怪物”（Spiegelman Monster），没有说清楚实验是在加了
RNA replicase的培养液中；这样经过足够代选择加入的RNA当然最后只剩下和RNA
replicase binding的部分，没什么稀奇；可这和所谓的”原始汤”环境有可比性吗？
氨基酸和其他生命的小分子（核酸、脂类）在地球早期严酷环境中产生（无论在空气、
海洋火山还是火山湖泊中）或者从陨石中带来，这是生命产生的前提，而米勒实验是证
明了这种自然产生的一种可能性。它的意义被作者一句话否定掉了？
又比如说到“黑烟囱”生命起源假说，说到最后是：“只需要用着白给的能量
发明膜就行了。”似乎已经无比简单了。可大哥，你是说要发明含ATPase的lipid膜吗
？大哥，ATpase是个如此复杂的蛋白，怎么说有就有了？而且就算有了，怎么才能遗传
啊？ 是得进化出一个RNA 的ATPase吧，... 阅读全帖 |
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i***l
发帖数: 1656 | 17 “不用Detergent，还是可溶的细菌膜蛋白”？
这会是每个人的都有疑问--为什么没有Detergent就能提纯？还有活性？
（你也没说怎么破碎的 哪里表达的，Assuming是大肠，超声波。）
我估计，可能极少数穿莫蛋白和细胞膜在破碎过程中形成MICELLS，然后过柱子时候被你
拿到了。如果NTA柱子挂了蛋白后变成黄色，或者你的Elute是浅黄色，就很有可能是有
膜成分共同被结合，洗脱了。当然如果及少量，也可能看不到颜色变黄。
不怕麻烦的话，可以挑个简单的实验 加温和的Detergent （OG，DM，等等温和但是CMC
大点儿的），看看结果是不是一样。
Pierce的手册里有很好的关于Detergent的介绍 |
|
i***l
发帖数: 1656 | 18 nod 过滤膜没意义。
其实就先Argue一下好了，根据你说的“这一类蛋白如果提取inverted membrane
vehicle也是有活性的”，再加楼上那个建议
：”
发信人: qianwang (oasis), 信区: Biology
这个蛋白只有两个跨膜区，可能没有detergent对蛋白的活性影响不大。解释一下就可
以了吧“
///另外，如果真要弄清楚，不怕麻烦，还可以吧你的Elute做个冷冻电镜（或者负染？
）看看，如果是
Micelles 会看到一个一个的
小球的。不知道别的手段能不能区分，也许Light Scattering 的机器也能？不过这些
就要工作量了 有时候看值得不值得。 |
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d********n
发帖数: 15 | 19 it's not degradation (proteases work less efficiently at ~90C, but will be a
disaster at ~55C at which proteases have reduced activity but protein
substrates begin to open structure and give much higher accessibility), but
precipitation because proteins lose native structure under heating. This is
why SDS or LDS loading bf needs to be added before heating. 1g sds binds to
14g proteins, 10ul sds loading bf is supposed to be enough wrap all proteins
in
the sample as they are so low.
the only probl... 阅读全帖 |
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A******y
发帖数: 2041 | 20 What's your reaction condition? You need to first make the activated NHS
ester PEG Phospholipid (or buy it if you can) in organic solvent. After
that you can do it in PBS buffer etc. If you run the EDC/NHS activation in
water solution, it won't work. |
|
n******g
发帖数: 662 | 21 I run the EDC/NHS activation in water solution. Can you explain the reason
for not working? Thanks.
in |
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A******y
发帖数: 2041 | 22 Water, H2O, is a nucleophile. It is competing with your NHS during the
activation. You will get the acid back. EDC activation need to be without
water. |
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n******g
发帖数: 662 | 23 My understanding is that EDC activation can be done in water (pierce website
, please search EDC).
DSPE does have ammonium ion. Do you think ammonium ion cause the problem?
form |
|
发帖数: 1 | 24 第一个建议真的很有用 我一定试试
A和B确实都是一次跨膜蛋白
我也非常怀疑是否是false positive
确实用的NP40 buffer 提全蛋白（7号针头抽吸）
但现在结果是A和B的全长和cleavage都作用
而且cleavage要比全长作用强很多 且cleavage在胞内部分
所以我想这样B cleavage是否就不算膜蛋白 也就不行成micelle
为了避免这种可能 所以做截段看作用位点 这样也能抛开膜蛋白特性
不管如何还是希望尽快确定是false还是true
RIPA |
|
k***i
发帖数: 662 | 25 August 6, 2007
Volume 85, Number 32
p. 9
Materials Chemistry
Tailor-Made Nanostructures
New methods offer precise control of polymeric micelles
Bethany Halford
CONTROLLING THE FORMATION of complex polymeric nanostructures is no easy task. As the polymer molecules self-assemble, they tend to form particles in an unruly mess of different shapes and sizes. Two new advances offer scientists the ability to tailor these properties, thereby offering nanostructure design strategies that could be |
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n*****g
发帖数: 4117 | 26 ☆─────────────────────────────────────☆
flyhigher (robbie tong) 于 (Wed May 13 18:38:09 2009) 提到:
发信人: flyhigher (robbie tong), 信区: Pharmaceutical
标 题: 哪个方向好一些？
发信站: BBS 未名空间站 (Wed May 13 18:35:14 2009)
1.protein delivery, by chemical conjugation or nanoparticle
2.anti-cancer drug delivery (small molecule), by polymer micelle
同一个组，但不知道哪个方向离industry更近一些？个人感觉后者现在有无数个lab都
在做，
前者好像搞的人还不太多，但不知道将来前景如何
谢谢！
☆─────────────────────────────────────☆
nanodog (上海狗狗) 于 (Wed May 13 18:43:39 2009) 提到: |
|
d****i
发帖数: 360 | 27 Two papers, please! Thanks a lot! E-mail: x**********[email protected]
Biofunctionalized magnetic-vortex microdiscs for targeted cancer-cell
destruction; Kim, D.-H., Rozhkova, E.A., Ulasov, I.V., Bader, S.D., Rajh, T.
, Lesniak, M.S., Novosad, V. 2010 Nature Materials 9 (2), pp. 165-171 1
Synthesis of hybrid gold/iron oxide nanoparticles in block copolymer
micelles for imaging, drug delivery, and magnetic hyperthermia; Kim, D.-H.,
Rozhkova, E.A., Rajh, T., Bader, S.D., Novosad, V. 2009 IEEE Tran |
|
d****i
发帖数: 360 | 28 One paper, please! Thanks a lot! E-mail: x**********[email protected]
Bioconjugate chemistry.
Tumor-Targeting, pH-Responsive, and Stable Unimolecular Micelles as Drug
Nanocarriers for Targeted Cancer Therapy
Xiaoqiang Yang, Jamison J. Grailer, Srikanth Pilla, Douglas A. Steeber and
Shaoqin Gong |
|
d****i
发帖数: 360 | 29 One paper please! Thanks a lot! E-mail: x**********[email protected]
Preparation and pharmacodynamics of stearic acid and poly (lactic-co-
glycolic acid) grafted chitosan oligosaccharide micelles for 10-
hydroxycamptothecin
Show Abstract Zhou, Y.-Y., Du, Y.-Z., Wang, L., Yuan, H., Zhou, J.-P.,
Hu, F.-Q. 2010 International Journal of Pharmaceutics 393 (1-2), pp. 144-
152 |
|
s**m
发帖数: 124 | 30 Fluorescence Behavior of Phenolic Compounds in Solutions Containing Micelles
.
http://www.informaworld.com/smpp/content~db=all~content=a768308185
Authors: Miriam R. de Morenoa; Robert V. Smitha
Affiliation: a Drug Dynamics Institute College of Pharmacy University
of Texas at Austin,
Austin, Texas
DOI: 10.1080/00032718308077167
Publication Frequency: 18 issues per year
Published in: Analytical Letters, Volume 16, Issue 20 1983 , pages 1633 -
1645
Subjects: Analytical Chemistry; Chemical Spe |
|
|
f******n
发帖数: 137 | 32 求一个2007 fall ACS polymer preprint 的全文.谢谢.
Supramolecular nanominietics: Replication of viruses, micelles, and other
soft, self-assembled nanoscale objects Context Sensitive Links
Author(s): Maynor B (Maynor, Benjamin), LaRue I (LaRue, Isaac), Rolland JP (
Rolland, Jason P.), Pandya AA (Pandya, Ashish A.)2, Hu ZK (Hu, Zhaokang),
Sheiko SS (Sheiko, Sergei S.), DeSimone J (DeSimone, Joseph) |
|
|
z******g
发帖数: 28 | 34 不知道够不够资格申eb1,希望多拿一些paper review 的机会。
我的专业是: polymer synthesis (free and controlled radical,insertion polym.
etc), amphiphilic polymers, micelles, organometallics.原意付一定报酬。
本广告长期有效，拜托大家有机会的时候可以关照我:) |
|
|
b*******u
发帖数: 75 | 36 搭车求问一下， DLS能测2nm以下的球形聚集物么？ 原来看见过有人说DLS能测普通表
活的micelle，有点不信...... 多谢！ |
|
|
w******e
发帖数: 953 | 38 Chen, F., Z. Jia, K.C. Rice, R.A. Reinhardt, K.W. Bayles and D. Wang, The
development of dentotropic micelles with biodegradable tooth-binding
moieties. Pharmaceutical Research, 2013. 30(11): p. 2808-2817. |
|
w******e
发帖数: 953 | 39 Chen, F., Z. Jia, K.C. Rice, R.A. Reinhardt, K.W. Bayles, and D. Wang, The
development of dentotropic micelles with biodegradable tooth-binding
moieties. Pharmaceutical Research, 2013. 30(11): p. 2808-2817. |
|
发帖数: 1 | 40 化学/高分子/材料 求推荐审稿机会
才完成PhD,准备开始申请绿卡,在这里向各位大牛求审稿机会
涉及的领域主要有polymer/macromoleculear chemistry, self-assembly, drug
delivery, organic electronic materials, hybrid materials, block copolymer,
stimuli-responsive polymer, micelles and hydrogels等
email:[email protected]
如需要更多信息（比如CV或者publication list）请email我，多谢多谢 |
|
x***a
发帖数: 409 | 41 【 以下文字转载自 RuralChina 讨论区,原文如下 】
发信人: micel (笨蛋笨蛋), 信区: RuralChina
标 题: 高赋文：“阿姨”——中国的佣人阶层
发信站: Unknown Space - 未名空间 (Sat Nov 15 16:57:16 2003) WWW-POST
中国思维网 www.chinathink.net 2003年11月15日 阅读数： 182 摘自:华尔街日报
生长于八十年代中国农村的王建龙(Wang Jianlong,音译)从未想到自己有朝一日也能使唤
上女佣。
现年25岁的王先生做工艺品生意。他回忆说﹕“过去家里根本雇不起女佣。富裕人家也没
有女佣。”
王先生说话之间﹐他家的女佣罗尤莲(Luo Youlian,音译)在一旁打扫他那空荡荡的三居室
公寓。然后﹐她收拾起昨天晚上的残羹剩饭﹐开始洗刷碗筷。身材矮小的她脸上刻着岁月
的痕迹。
罗尤莲每周工作七天﹐每天工作十小时。她总是忙个不停－－扫地﹑拖地﹑擦窗﹑洗熨衣
服﹐而每个月的工资约为50美元。王先生说﹕“她的报酬很合理。”王先生是通过一家中
介机构雇用罗尤莲的。
目前﹐中国出现 |
|
x*o
发帖数: 1037 | 42
STM or AFM can image 3D object at high resolution, can't they?
blotting,
in
of
it
a
1,
Phys. |
|
b*o
发帖数: 47 | 43
我把那篇文章看了好几遍，还是不明白他们的共聚物是怎么交联的。Or just
micell aggregation? Please help me understand. Thanks.
have
Instead,
two
systems
engineering.
the |
|
b**s
发帖数: 589 | 44
我觉得大概是这样的
这种两亲高分子与普通的两亲高分子的区别在于
1. 因为它是多肽,所以它可以互相缠绕形成蛋白质样的结构，所以憎水的链节在这里
自组装，起到交联点的作用，而且交联强度的顺序是a-helix> b-strand > random coil
2. 而亲水的链节是带电的，互相排斥，无法靠近
所以一吸一斥，使得高分子链既不能聚集成micell,又不能分散在水里形成溶液，以至
於这种两亲高分子在水中形成独特的hydrogel而不是胶体。
small
a
hydrophobic
could
that |
|
b*o
发帖数: 47 | 45
里
coil
至
多谢讨论。我明白多月太自组装充当一个交联点，但另一个交联点呢？还是倾向于
micell aggregation, something like in the PEG-b-PLGA case. |
|
b*****y
发帖数: 19 | 46 Let's talk something about it. |
|
|
p*****d
发帖数: 40 | 48 like patterned surface.
phase shift is mainly determined by contrast in viscoelastisity, proved by
Garcia and other guys |
|
b**s
发帖数: 589 | 49 'polymer therapeutics'. (Polymer therapeutics are nano-sized
medicines.)，估计大量的公司将从中诞生
Polymer therapeutics are nano-sized medicines. The definition encompasses
polymeric drugs (polymeric molecules that are biologically active in their own
right); polymer–drug conjugates; polymer–protein conjugates; polymeric
micelles to which drug is covalently bound; and polymeric non-viral vectors
for gene delivery.
1。Biologically active polymers and polymeric sequestrants are entering
routine clini |
|
w********h
发帖数: 12367 | 50 Bates and Lodge already published something about this bah.... |
|
