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全部话题 - 话题: monolayer
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发帖数: 1
1
名字叫舍恩。
德国科学家。不少Nature,Science。后来均被撤稿。
以下摘自维基百科。将军们怎么看这位白人科学家?据一位王姓的华人知名professor
透露,他去演讲,有个欧洲白人老头老是说华人professor的文章是造假造出来的数据
,华人教授气都气死了。
【舍恩事件】
舍恩事件是一起于2002年揭发的大规模学术论文造假丑闻。事件主角扬·舍恩 (Jan
Hendrik Schön) 1970年生于德国费尔登,1997年获康斯坦茨大学物理学博士学位
并留校任教,1998年加入贝尔实验室,于贝尔特拉姆·巴特洛格教授指导下做材料学研
究,2001年成为正式员工。之后的4年里,他与20多人合作在许多大型国际期刊上发表
了超过80篇论文,在《自然》和《科学》上发表的第一作者署名的论文就有17篇。这些
论文在当时被认为于分子电路、分子半导体、有机激光、高温超导和纳米科技领域取得
突破进展,震动了整个科学界。2001年,他获得了德国最重要的科学奖项之一——奥托
·克隆-韦伯银行奖。
舍恩最出名的“成就”是于2001年11月发表的单分子场效应管。他称在两层金电极之间
用含硫的有... 阅读全帖
w******e
发帖数: 953
2
来自主题: Faculty版 - 某会计牛人出事了!
At least Bao zhe nan survived after her Schön scandal.
Withdrawn journal papers
On October 31, 2002, Science withdrew eight papers written by Schön:[14]
• J. H. Schön, H. Meng, Z. Bao (2000). "An Organic Solid State
Injection Laser". Science 289 (5479): 599–601. Bibcode 2000Sci...289..599S
. doi:10.1126/science.289.5479.599. PMID 10915617. (Retracted)
On February 24, 2003, Applied Physics Letters withdrew four papers written
by Schön:[17][18][19][20]
• J. H.... 阅读全帖
a***r
发帖数: 981
3
来自主题: Faculty版 - [合集] 某会计牛人出事了!
☆─────────────────────────────────────☆
funhunt (funhunt) 于 (Sat Feb 2 00:03:06 2013, 美东) 提到:
有读者致信某顶级会计刊物,指出此做行为研究的牛人一篇两年前发表的文章数据有明
显错误,此牛人承认疏失。编辑怀疑可能还有其他错误,坚持要求作者提供原始数据。
牛人以和提供数据的公司有保密协定为由拒绝提供。编辑不让步,牛人只好自愿撤稿。
牛人所在某私立学校马上展开调查,不久此牛人辞职。估计此牛人学术生涯也完了。
估计此人多年以来的文章合作者也可能正在惶惶不可终日。
学术界水也不浅,震惊啊!
☆─────────────────────────────────────☆
huaqizhiqing (Huaqizhiqing) 于 (Sat Feb 2 10:02:56 2013, 美东) 提到:
能不能说的详细一点?
☆─────────────────────────────────────☆
whoiwere (dddddddd) 于 (Sat Feb 2 10:24:... 阅读全帖
B*******e
发帖数: 3882
4
看 了一下 ieee 的评论, 查了一下nature 原文的摘要。 这个报道有点问题。
这是原摘要:
“ 。。。。Graphene has attracted large interest in photonic applications
owing to its promising optical properties, especially its ability to absorb
light over a broad wavelength range, which has lead to several studies on
pure monolayer graphene-based photodetectors. However, the maximum
responsivity of these photodetectors is below 10 mA W−1,
which significantly limits their potential for applications. Here we report
high photore... 阅读全帖
a*****c
发帖数: 53
5
Rise to prominence[edit]
Schön's field of research was condensed matter physics and
nanotechnology.[3] He received his Ph.D. from the University of Konstanz in
1997. In late 1997 he was hired by Bell Labs, located in New Jersey, United
States. There, he worked on electronics in which conventional semiconducting
elements (such as silicon) were replaced by crystalline organic materials.
Specific organic materials can conduct electrical currents, and in a so-
called field-effect transistor (a ... 阅读全帖
m******i
发帖数: 834
6
来自主题: NanoST版 - C60单层膜的制备zz
Interfacial Supramolecular Self-Assembled Monolayers of C60 by Thiolated -
Cyclodextrin on Gold Surfaces via Monoanionic C60
Release Date: 28-Mar-2007; (Article) DOI: 10.1021/ja067056z
The supramolecular self-assembled monolayers (SAMs) of C60 by thiolated -
cyclodextrin (CD) on gold surfaces were constructed for the first time using
C60 monoanion. The results indicate that monoanionic C60 plays a crucial
role in the formation of the C60-containing self-assembled monolayers. The
generation of
C*I
发帖数: 4736
7
一直在误导,现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人,必须
经过一个中间宿体性的其它动物才能传染给人。所以,病毒发生后,就故意误导全国人
民去海鲜市场找证据,找其它野生动物的麻烦。 而且还是病毒所去找的,找完了还装
模做样化验呀,分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
,一直不敢说,说了其它相关已经在人就会去早那种动物监测。 所以压根不说,打马
虎眼。
事实上,早在2013 年,就是这个武汉病毒研究所,已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒,其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体,并能够感
染人的细胞。对此新发现,武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”,以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说,从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus, 可以不经过
其它受体/宿体,而直接传染给人。 他们... 阅读全帖
C*I
发帖数: 4736
8
一直在误导,现在还在误导。 说说明corona virus 不可以从蝙蝠直接传染给人,必须
经过一个中间宿体性的其它动物才能传染给人。所以,病毒发生后,就故意误导全国人
民去海鲜市场找证据,找其它野生动物的麻烦。 而且还是病毒所去找的,找完了还装
模做样化验呀,分离呀什么的。最后把责任全部推给了海鲜市场的动物。可是那种动物
,一直不敢说,说了其它相关已经在人就会去早那种动物监测。 所以压根不说,打马
虎眼。
事实上,早在2013 年,就是这个武汉病毒研究所,已经从来自云南的蝙蝠身上所携带
的corona virus中分离出第一株蝙蝠SARS类似样的冠状病毒的活病毒,其中就包含了类
似于S类型的基因。从而证实这株病毒能够使其接受和SARS病毒相同的受体,并能够感
染人的细胞。对此新发现,武汉病毒所还把它以武汉病毒研究所的英文简称命名“WIV1
”,以彰显这一发现的重要价值和属于自己第一个发现的巨大研究成果。这个成果刊载
于2013年11月的《自然》杂志。
就是说,从云南弄回来的这种蝙蝠所携带的类似于sars的 corona virus, 可以不经过
其它受体/宿体,而直接传染给人。 他们... 阅读全帖
C*I
发帖数: 4736
9
Published: 30 October 2013
Isolation and characterization of a bat SARS-like coronavirus that uses the
ACE2 receptor
Xing-Yi Ge, Jia-Lu Li, Xing-Lou Yang, Aleksei A. Chmura, Guangjian Zhu,
Jonathan H. Epstein, Jonna K. Mazet, Ben Hu, Wei Zhang, Cheng Peng, Yu-Ji
Zhang, Chu-Ming Luo, Bing Tan, Ning Wang, Yan Zhu, Gary Crameri, Shu-Yi
Zhang, Lin-Fa Wang, Peter Daszak & Zheng-Li Shi
Nature volume 503, pages535–538(2013)Cite this article
Abstract
The 2002–3 pandemic caused by severe acute respirator... 阅读全帖
m*******r
发帖数: 66
10
来自主题: Germany版 - 也说guten tag
发信人: monolayer (炼金士), 信区: Overseas
标 题: 也说guten tag [原创]
发信站: 南京大学小百合站 (Tue Nov 5 17:36:03 2002), 站内信件
【 以下文字转载自 Deutsch 讨论区 】
【 原文由 monolayer 所发表 】
那是2000年的初夏
刚得知自己将前往德国,
积极的去买了个光盘学德语,
学会了Guten Tag.
取办理签证了,上午10点,
我说:guten tag.
洋鬼子唧唧咕咕,我傻了。
赶紧说只会英语。
回来,在德国,我才了解,
上午10点,应该还是
Guten morgen..
谨以此纪念我的德国旅,
轻轻的,我又要走了。
N******y
发帖数: 110
11
来自主题: Chemistry版 - Paper help!! Thanks!
求帮忙下这篇Paper,多谢!!
A Study of Au/C Nanoparticles with Pt Monolayer and Sub-Monolayer
Electrocatalysts for Ethanol Oxidation Reaction,ECS Trans. 2013 volume 58,
issue 1, 1733-1736
链接是 http://ecst.ecsdl.org/content/58/1/1733.short
a***n
发帖数: 578
12
来自主题: Macromolecules版 - SAM as biosensor: sound fun
Self-assembled monolayers as a tunable platform for biosensor applications
Chaki NK, Vijayamohanan K
BIOSENSORS & BIOELECTRONICS
17 (1-2): 1-12 JAN 2002
Abstract:
Considerable attention has been drawn during the last two decades to
functionalize noble metal surfaces by forming ordered organic films of few nm
to several hundred-nm thickness. Self-assembled monolayer (SAM) provides one
simple route to functionalize electrode surfaces by organic molecules (both
aliphatic and aromatic) containing fr
m*******r
发帖数: 66
13
来自主题: Macromolecules版 - 球球TIP之继续问..
发信人: monolayer (炼金士之ISU), 信区: NanoST
标 题: 球球TIP之继续问..
发信站: 南京大学小百合站 (Tue Aug 19 12:29:31 2003)
谢谢大家对球球AFM的Tip的讨论,现在问个相关的物理力学问题.
假设现在我把球球tip放在一悬空薄膜表面,然后做力-距离曲线,
根据所得的数据,可以得到力的大小和膜的形变距离,
问题是,如何由这些数据计算薄膜的力学性质,如:杨氏模量?
由于本人主要从事炼金(chem)的工作,对材料物理了解甚少,望各位多多
帮助.monolayer在此多多致谢啦.
s*********s
发帖数: 18
14
Thanks for your good explains and good comments.
The following responds to your one by one
单层graphene谁能加gate打开gap???
As mention above,i didn't mention the conclusion that monolayer graphene can
open band gap. But researcher have been shown that monolayer graphene nano-
mesh and nano-ribbon can open band gap under the applied electric field.
1.强磁场下当然是形成朗道能级啦,不过graphene有零能的LL,弱场下应该没有gap
The effect of the applied perpendicular magnetic can induce the splitting of
Landau Level and the zero-energy L... 阅读全帖
l*****g
发帖数: 56
15
呵呵, 看看science上那个news写的吧.
"it's virtually impossible for me to believe that some of this wasn't made up"
Lieber这种话都说出来了.
Schon 以后不管怎么说都会比较难混了. 其实他做的过分了, 偶第一次看science 12月
的那篇paper的时候就觉得有点不对了, 和nature的比较fig完全一样, 后来加了个
correction说是加一个0.1的factor, 那有这么巧的. 按他的说法, nature的monolayer,
science的是diluted看做single molecule的, 很简单的直觉上也应该知道monolayer更
接近bulk一些. 就算不考虑物理内涵, 实验上普遍观察到的current leakage,
两个结果就不应该是简单的线性关系.
偶现在做理论, 上个礼拜跟一个postdoc讨论molecular transistor的问题, 他science
的那篇paper很难用理论解释, 因为外加小电场对分子轨道的改变微乎其微.
后来pos
s*****r
发帖数: 43070
16
来自主题: Military版 - 女博士果然是风情万种啊
英文水平不太出彩啊,读博士害死美女
I'm an overseas student from China and I've been in England for 4 years.
This is the third year of my PhD under the supervision of Fiona Meldrum. My
project is about carrying out crystallisation in small volumes, using a
Crossed-Cylinder apparatus and with addition of various additives. I have
begun to explore the use of this system to study CaSO4 precipitation in bulk
solution and confinement. And it appears that amorphous calcium sulfate (
ACS) and metastable phase, hemihydra... 阅读全帖
C*****y
发帖数: 510
17
☆─────────────────────────────────────☆
Glion (apprentice) 于 (Sat May 19 20:43:53 2007) 提到:
要去做POSTDOC,想找老板办H1, 可能会自己出钱,在POSTDOC版有人说除了交给政府的那
个$2000左右,老板还需要向学校交钱. 请问,是这样么? 如果是,要交多少??? 多谢了~~
☆─────────────────────────────────────☆
monolayer (炼金士) 于 (Sat May 19 22:44:15 2007) 提到:
Yes. As I know, something like $500 to 1000, to HR from the paperwork. It
could be varied univ by univ.
C*****y
发帖数: 510
18
来自主题: Faculty版 - [合集] 关于H1-B visa的问题
☆─────────────────────────────────────☆
ideaguarder (deepidea) 于 (Fri May 18 15:20:26 2007) 提到:
我现在是H1-B,刚刚开始生效, 有效期三年,可是今年九月分开始要到新的学校上班,请
问H1是重新申请呢还是transfer, 如果是transfer,怎么个办法,什么时候开始办, 应该
和新学校的什么人联系? 如果是重新申请,是不是现在就应该申请了,和什么人联系.
谢谢
☆─────────────────────────────────────☆
monolayer (炼金士) 于 (Fri May 18 15:27:12 2007) 提到:
H1-B transfer is the same paperwork as 重新申请. The good thing for transfer
is that you can work after you submit the petition (no need fot wait the
approve.
☆────────────
C*****y
发帖数: 510
19
来自主题: Faculty版 - [合集] 大家怎么要求加工资
☆─────────────────────────────────────☆
letterman (爱谁谁) 于 (Thu May 24 10:36:29 2007) 提到:
在neogociate的时候,用什么理由?
would you please raise my salary by 3k?
it is kind of begging.
any suggestion
☆─────────────────────────────────────☆
monolayer (炼金士) 于 (Thu May 24 12:08:58 2007) 提到:
FYI
you can kind of complain about the "expensive house" (if it is) and
expensive other expense(if there are, such as private school for kids, LD
can not find job easily, etc.)
good luck.

☆──────────────────────
C*****y
发帖数: 510
20
☆─────────────────────────────────────☆
glassdream (dashao) 于 (Fri May 25 13:12:26 2007) 提到:
5月7日结束校园面试,
系主任曾经在11日打过电话说因为有些学校office的人不在,还要等等。
但强调说会隔几天再打电话来,通知我的application status.
结果这都面试完三个星期了,
就再也没有任何消息了。
我这些天简直是度日如年,等得难受死了。
请问大家以及前辈们,
我要给这位系主任发信问问怎么回事吗?
(btw,学校是semester制的州立大学)
☆─────────────────────────────────────☆
monolayer (炼金士) 于 (Fri May 25 13:25:57 2007) 提到:
You can ask for sure. maybe you are not their 1st choice....good luck.
☆─────────────────────────────────────☆
glassdr

发帖数: 1
21
清华大学物理系周树云研究组诚聘博士后
岗位介绍
研究方向:新型二维材料及超快动力学
薪酬待遇:我们为你的科学研究和下一步的职业发展提供优越的条件,包括前沿的研究
课题和先进研究技术、广泛的出国交流和合作机会等。工资范围为每年18万至30万,根
据个人能力面议;依据清华大学博士后人员政策,提供交通方便的校内住宿及子女入学
待遇。特别优秀的申请者,我们将支持申请清华大学“彭桓武”博士后;对于满足要求
的海外博士毕业生,将支持申请“博士后国际交流计划”引进项目(资助标准为30万/
年)。
岗位要求:热爱科学研究,爱钻研,有独立科研工作能力和工作责任心。已获得或即将
获得博士学位,凝聚态实验物理经历背景,有超快光谱或样品生长、表征及物性测量研
究背景者优先考虑。
申请材料:详细的个人简历(包括个人教育和工作经历、科研工作概述、论文发表情况
及其它成果),推荐人的联系方式。
联系人:周树云 [email protected]/* */
研究组导师简介
周树云,2007年博士毕业于美国加州大学伯克利分校,之后在劳伦兹伯克利国家实验室
先后任博士后研究员和项目科学家,2012年入选青年千... 阅读全帖

发帖数: 1
22
Postdoc and Professorships in ICQMS
Shanghai University http://www.shu.edu.cn is seeking outstanding investigators for full-time tenure-track positions in Computational Materials Science and Condensed Matter Physics, at the ranks of Postdoc, Assistant Professor through Full professor. Candidates will be part of the newly created International Centre for Quantum and Molecular Structures (ICQMS, http://icqms.shu.edu.cn) and be eligible for parallel Adjoint Appointments at Department of Physics, ... 阅读全帖
k******6
发帖数: 7
23
Postdoctoral Research Associate Position in
Synthesis and Characterization of Two-Dimensional Organic Materials
Center for Nanophase Materials Sciences, Oak Ridge National Laboratory
Reference Code: ORNL12-76-CNMS
Project Description
The Functional Hybrid Nanostructures group at the Center for Nanophase
Materials Sciences (CNMS) at Oak Ridge National Laboratory is seeking to
fill a postdoctoral research position in a project focused on the synthesis
of two-dimensional (2D) materials, similar to ... 阅读全帖
k******6
发帖数: 7
24
Postdoctoral Research Associate Position in
Synthesis and Characterization of Two-Dimensional Materials
Center for Nanophase Materials Sciences, Oak Ridge National Laboratory
Reference Code: ORNL12-76-CNMS
Project Description
The Functional Hybrid Nanostructures group at the Center for Nanophase
Materials Sciences (CNMS) at Oak Ridge National Laboratory is seeking to
fill a postdoctoral research position in a project focused on the synthesis
of two-dimensional (2D) materials and the
characteriza... 阅读全帖
g********e
发帖数: 1638
25
195 黄佳
(chem.) 男 1980/11/23 同济大学 工程与材料科学 2009年05月毕
业于[美国]约翰霍普金斯大学 [美国]马里兰大学 博士后研究员/Research
Associate
这位自己有简历在网上,
http://www.linkedin.com/pub/jia-h/8/b10/3b9
第一作者文章很少,有一个JACS,
Publications
Applications of ZnO in organic and hybrid solar cells
Energy & Environmental Science
2011
Authors: Jia H.
(1) Huang, Jia*; Yin, Zhigang; Zheng, Qingdong. Energy & Environmental
Science. (2011) Accepted, *corresponding author
Polymeric Semiconductor/Gra... 阅读全帖
w*****g
发帖数: 125
26
manuscript from Analyst @RSC, on nano-patterned self assembled monolayer and
MALDI-MS
PM me if interested with your CV.
c*******3
发帖数: 98
27
I would like to review ZnO growth manuscript.Thanks!
My CV is as follows:
JINGBO CHANG
Laboratory of Nanotech. for Sustainable Energy and Environment University of
Wisconsin-Milwaukee, 3200 North Cramer Street Milwaukee, WI 53211, USA
www.researchgate.net/profile/Jingbo_Chang/publications/
PERSONAL DETAILS
Date of Birth: 28th Sep. 1979 Gender: Male
Nationality: China Marital Status: Married
Tel. +1- 414- 688-1383 E-mail: c*****[email protected]
EDUCATIONS
☼ Post doc, major in Mechanical Engineerin... 阅读全帖
c*******3
发帖数: 98
28
来自主题: Immigration版 - Gone! 审稿机会推荐
JINGBO CHANG
Laboratory of Nanotech. for Sustainable Energy and Environment University of
Wisconsin-Milwaukee, 3200 North Cramer Street Milwaukee, WI 53211, USA
Research Highlights
 Graphene/SWNTs FET based platform for biosensor and water sensor
applications.
 Graphene based hybrid materials for high-performance lithium-ion
battery applications.
 High-performance photoresponse from SWNT/ZnO heterojunctions for UV
sensor
and solar cell applications.
 High conducti... 阅读全帖
t*****r
发帖数: 117
29
【 以下文字转载自 German 讨论区 】
【 原文由 monolayer 所发表 】
http://dict.leo.org/
这里的鬼子常用这个!
a*******i
发帖数: 11664
30
真是服了你们了
就是一个简单的压强问题,和monolayer黏性有神马关系。
d***e
发帖数: 243
31
来自主题: Biology版 - Re: 细胞传代求助
Trypsin, pepsin 等对LH receptor影响不大{Gospodarowicz 1973}. 所以也可能是其它
的一些原因:cells condition, monolayer overlapping之类的问题.当然commercial
trypsin function本身就比较复杂,有时有些人就用较纯化或重组的trypsin和胶原酶之
类成份确定的混合物去消化。
我的感觉是你是否让你的宝贝细胞刚长得90%confluence后,hanks washing triple,再用
0.05%trypsin在不同温度(Rm, 36)消化一下看有没有提高。
实在不行就定些Cell Dissociation Buffer or Enzyme Free Dissociation
Reagents(60% confluence),但我总认为是花拳秀腿,但也许就对你的实验管用。
另外培养界面,polypropylene, glass, teflon-coated 都是option,但我对这些不熟悉
。可能还有几个较流氓的方法,冰水超声,内置tiny cover slides, 瞬时低渗
m******e
发帖数: 18
32
来自主题: Biology版 - U87MG cell
Did you add extra glutamine to your medium? U87-MG grows as polylayer not
monolayer, please check with cell counting instead of confluence.
d******r
发帖数: 124
33
来自主题: Biology版 - 求高人鉴定一下
最近在培养neuron,今天是第四天,发现很多细胞聚集在一起,中间的看起来分化得也
不好,neurite很短,如图所示。请高人鉴定一下,这些是不是neuron?可能是什么原
因造成的?我是从E18 mouse embryo分离的neuron,用波片倒扣在astrocyte
monolayer上培养。medium用的是neurobasal+B27。谢谢!
a*l
发帖数: 2685
34
来自主题: Biology版 - paper help !!
is it this one? The Caco-2 cell monolayer: usefulness and limitations.
Expert Opin Drug Metab Toxicol. 2008 Apr;4(4):395-411.
http://www.sendspace.com/file/rllq23
s*****e
发帖数: 222
35
来自主题: Biology版 - 现在还可以求paper么?
大学的电脑也找不到阿
Role of metastatic potential in the adhesion of human breast cancer cells to
endothelial monolayers
http://www.ncbi.nlm.nih.gov/pubmed/10928052
信箱:
c****************[email protected]
多谢多谢
s*****e
发帖数: 222
36
来自主题: Biology版 - paper help please, thank you!
请问能帮我找一下么?
大学的电脑也找不到阿
Role of metastatic potential in the adhesion of human breast cancer cells to
endothelial monolayers
http://www.ncbi.nlm.nih.gov/pubmed/10928052
信箱:
c****************[email protected]
多谢多谢
l**********1
发帖数: 5204
37
来自主题: Biology版 - 有人养过HL-1细胞吗?
RE:
孵蛋的学第/妹 giantbird05 (大鸟)
Did you use Claycomb-medium ?
if not, please use it
Claycomb-medium (Sigma, Germany)
plus fibronectin/gelatine-precoated cell culture flasks.
more details about stuff protocol
pls go to
PLoS One. 2012; 7(2): e31669.
PuMeb link:
//www.ncbi.nlm.nih.gov/pmc/articles/PMC3285183/
citation:
>Cell culture
>The HL-1 cardiomyocytes (murine atrial tumor cell line) were
maintained in monolayer culture with Claycomb-medium (Sigma,
Germany), supplemented with 10% fetal calf serum... 阅读全帖
l**********1
发帖数: 5204
38
来自主题: Biology版 - 有人养过HL-1细胞吗?
注意media Lot的原产国
最重要的一个 可能是
去甲肾上腺素
1% norepinephrine (Sigma-
Aldrich, Germany)
from
北美的有的牛来源的原料
其中的疯牛病因子 and 瘦肉精因子等比德国的EU标准高
以下的所有东西 订货 按原产国来 订货 then try again.
Cell culture
>The HL-1 cardiomyocytes (murine atrial tumor cell line) were
maintained in monolayer culture with Claycomb-medium (Sigma,
Germany), supplemented with 10% fetal calf serum (SAFCBiosciences,
USA), 1% penicillin/streptomycin, 1% glutamax
(both Invitrogen, Germany) and 1% norepinephrine (Sigma-
Aldrich, Germany) in an incubato... 阅读全帖
m*********r
发帖数: 49
39
最近在用invitrogen的bac to bac做表达,gene克隆到bacmid了之后,现在在转染(
transfection, 不知道翻译准不准)。前一次做的感染细胞放了一星期,monolayer都
要100%了,还没死,怀疑感染效率低。现在试第二次,已经转染后72-96小时了,发现
转染了bacmid的细胞除了长得比不加bacmid的对照长的慢点,好像很难看出来
invitrogen册子上说的细胞变大,出芽(这个我尤其难分辨)什么的(我平时也就用
200X inverted microscope观察),大家有比较明显的感染细胞和不感染细胞的对照图
推荐我瞧瞧么?我不想一直等到细胞dislodge了,或者裂解了才能知道感染成功没有。
f******g
发帖数: 1003
40
Reprogramming human endothelial cells to haematopoietic cells requires
vascular induction
Nature
Generating engraftable human haematopoietic cells from autologous tissues is
a potential route to new therapies for blood diseases. However, directed
differentiation of pluripotent stem cells yields haematopoietic cells that
engraft poorly. Here, we have devised a method to phenocopy the vascular-
niche microenvironment of haemogenic cells, thereby enabling reprogramming
of human endothelial cells in... 阅读全帖
L****T
发帖数: 169
41
胚胎干细胞领域的真正大牛(不过好像比较低调)之一,博士后期间以及独立PI后做出
了很多里程碑性质发现:cell fusion,BMP4,monolayer neural differentiation;
naive pluripotency,1st rat ESC and knockout rat等。
U**s
发帖数: 3390
42
the cell density is too high. you may try the formaldehyde crosslink in 10ml
growth medium (w/o serum; 1% formaldehyde) in falcon tubes.
If you grow adherent cells in monolayers, it's better to perform the
crosslink in petri dishes.
The basic principle is that: you want to take a snapshot of all kinds of
interactions with the minimum disturbance to the cells.
s********r
发帖数: 312
43
分化主要两种方法, EB或者monolayer. 要看你分化成哪种细胞。
b*****r
发帖数: 29
44
来自主题: ChemEng版 - 招化学化工方向的学生啦
代朋友发帖
1. Research Assistantship (RA) for a PhD Student in Mechanical Engineering
An NSF-funded research assistant position is available for a new PhD student
in Mechanical Engineering at the State University of New York (SUNY) at
Binghamton. The research is focused on materials printing using a hybrid
inkjet-electrospray technique. The goal is to print highly-ordered
monolayers of functional nanoparticle inks at high throughput. The student’
s research will be experimental; however, close collab... 阅读全帖
b*****r
发帖数: 29
45
来自主题: ChemEng版 - 招化学化工方向的学生啦
代朋友发帖
1. Research Assistantship (RA) for a PhD Student in Mechanical Engineering
An NSF-funded research assistant position is available for a new PhD student
in Mechanical Engineering at the State University of New York (SUNY) at
Binghamton. The research is focused on materials printing using a hybrid
inkjet-electrospray technique. The goal is to print highly-ordered
monolayers of functional nanoparticle inks at high throughput. The student’
s research will be experimental; however, close collab... 阅读全帖
s****e
发帖数: 2934
46
做了一个silane的合成。就是可以做monolayer的那种functionalized
trichlorosilane,单投合成。
s********m
发帖数: 748
47
来自主题: Chemistry版 - 问一个关于fluorescence sensor的问题
谢谢回复。那请问如果是做一个self-assembled monolayer,上面接荧光分子那种检测
是不是fluorometer就可以了呢?比如说用来检测离子,想观察bind离子前后的荧光强
度差别,可以直接用fluorometer吗?
l********7
发帖数: 110
48
来自主题: Chemistry版 - Paper help. Thanks.
Diacetylene polymerization in self-assembled monolayers: influence of the
odd/even nature of the methylene spacer
Polyer, 2000, 41, 8113-8119
l********7
发帖数: 110
49
来自主题: Chemistry版 - paper help!! thanks
Enhanced Photocurrent Generation by Electron Hopping through Regularly-
Arranged Chromophores in a Helical Peptide Monolayer
Tomoyuki Morita, Kazuyuki Yanagisawa and Shunsaku Kimura
Polymer Journal, 2008, 40, 700-709
My email is l*****[email protected].
Thanks.
y*******n
发帖数: 127
50
来自主题: Chemistry版 - 请教一个溴甲基与胺的反应
小弟有个想法,希望能在硅片上长上一层伯胺基的monolayer:先用LB法制备长上一层
溴代异丙酰胺,然后与spermine或氨的乙醇溶液反应产生伯胺基,诸位帮忙看看这个思
路靠谱不?
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