l**********1
发帖数: 5204 | 1 Sure
Sampling Tumor gDNA can not satisfied
Illumina high density SNP arrays while it below 5 ng even from 2015
now 2010 is 200 ng one sample
pls refer:
We also routinely run the high density array technique to genotype up to 1
million snps per subject. The available platform is from Illumina and is
able to generate genotypes for up 300-750 subjects per week depending on the
type of array chosen.
Required material 200 ng DNA per sample
Reaction format 96 wells
Detection machine iScan b... 阅读全帖 |
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w***b
发帖数: 89 | 2 Genome-wide expression profiling implicates a MAST3-regulated gene set in
colonic mucosal inflammation of ulcerative colitis patients
Catherine Labbé MSc1,2,
Gabrielle Boucher MSc1,
Sylvain Foisy PhD1,
Azadeh Alikashani MSc1,
Herbert Nkwimi MSc1,
Geneviève David BSc1,2,
Mélissa Beaudoin MSc1,
Philippe Goyette PhD1,
Guy Charron PhD1,
Ramnik J. Xavier MD3,4,
John D. Rioux PhD1,2,*
Article first published online: 12 OCT 2011
DOI: 10.1002/ibd.21887
Inflamm... 阅读全帖 |
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n***3
发帖数: 663 | 3 Nat Biotechnol. 2012 Aug 26. doi: 10.1038/nbt.2344. [Epub ahead of print]
Polyethyleneimine is a potent mucosal adjuvant for viral glycoprotein
antigens.
please send the paper to e*********[email protected]
Thanks! |
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n***3
发帖数: 663 | 4 Mucosal Immunol. 2013 Mar;6(2):235-43. doi: 10.1038/mi.2012.63. Epub 2012
Jul 4.
Nod1, but not the ASC inflammasome, contributes to induction of IL-1β
secretion in human trophoblasts after sensing of Chlamydia trachomatis.
Please send the paper to e*********[email protected]
Thanks so much! |
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y******8
发帖数: 1764 | 5 Abbreviated Journal Title ISSN {2012} Total Cites Impact Factor
5-Year Impact Factor Immediacy Index {2012} Articles Cited Half-
Life Eigenfactor Score Article Influence Score
CA-CANCER J CLIN 0007-9235 13722 153.459 88.55 27.04 25
3.3 0.0517 29.478
NEW ENGL J MED 0028-4793 245605 51.658 50.807 12.667 360
8 0.65776 21.494
REV MOD PHYS 0034-6861 35720 44.982 51.882 6.478 46 10
0.13048 32.634... 阅读全帖 |
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h********1
发帖数: 157 | 6 转个nature的招聘广告，看看下面这个“ＮＯＮ-Ｃhinese Ｎative”是什么意思，感
情中国本土人在国内还要受歧视了
Tsinghua University Immunology Faculty
Tsinghua University has made a strong commitment to building an immunology
program of international distinction and is recruiting tenure-track faculty
members in the broad immunology field. New laboratories will be located in
brand-new research buildings that feature state-of-the-art flow cytometers,
imaging facilities (multi-photon, TIRF, and atomic force microscopies),
proteomics core,deep se... 阅读全帖 |
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d****t
发帖数: 65 | 7 Functional profiling of the gut microbiome in disease-associated
inflammation
Metabolic Alterations to the Mucosal Microbiota in Inflammatory Bowel
Disease
发到s*****[email protected]
多谢 |
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d****t
发帖数: 65 | 8 Functional profiling of the gut microbiome in disease-associated
inflammation
Metabolic Alterations to the Mucosal Microbiota in Inflammatory Bowel
Disease
发到s*****[email protected]
多谢 |
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s******y
发帖数: 28562 | 9 请具体看他们的数据，而不是看最后的口号结论。只看标题和结论是小学生的做派。
这个文章的数据通篇在说细胞方面的影响是存在的，但是因为对于抗体以及cytokines
的测量结果不符合简单体液免疫模型应该推导出来的结果。所以他们最后下了一个“没
有影响”的结论。
我本来不想针对他们那篇文章评论过多，因为我本身也不是搞免疫的专门人士（只不过
碰巧知道得比较多，所以啥都是个半瓶水），所以我怕说多错多然后被人拿来攻击。但
是现在看来我有些过于高估在这个帖子系列下发言的人的素质了，有些估计根本不是搞
生物的？所以我只好赶鸭子上架多讲几句吧。希望专门做免疫的同学如果发现我那里讲
错了，请拍得轻一些。
Bt protein 的一个潜在生物毒性，就是杀死肠道上皮细胞，导致上皮层异常脱落，这
样皮下组织直接受到肠液的刺激，就会产生非特异性的炎症。如果是这种情况的话，一
个常见的临床表征就是Monocytes异常升高（这个被那个文章的作者观察到了）。但是
肠道发炎其实属于黏膜免疫的范畴，不能直接套用体液免疫的指标。尤其对于慢性黏膜
炎症，测什么cytokine 很难看出什么来，去测Bt protein 的特异... 阅读全帖 |
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l********n
发帖数: 260 | 10 Rank full journal title Total Cites IF
1 ca-a cancer journal for clinicians 18,594 115.84
2 new england journal of medicine 268,652 55.873
4 lancet 185,361 45.217
6 nature biotechnology 45,986 41.514
7 nature 617,363 41.456
12 nature materials 64,622 36.503
13 jama-journal of the american medical association 126,479 35.289
14 nature reviews immunology 28,938 34.985
15 nature nanotechnology 34,387 34.048
16 ... 阅读全帖 |
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s*********y
发帖数: 579 | 11 J of autoimmunity竟然都8分多了，Mucosal Immunology也7分多，JACI 12分上下，这
些都是导致JI越来越差的原因吧？不明白JEM为什么上不去。 |
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p**t
发帖数: 160 | 12 Mucosal Immunology还不错，很多做粘膜免疫做的好的在上面发paper。 |
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m*****e
发帖数: 129 | 13 Rank Full Journal Title Total Cites Journal Impact Factor
1 CA-A CANCER JOURNAL FOR CLINICIANS 20,488 131.723
2 NEW ENGLAND JOURNAL OF MEDICINE 283,525 59.558
3 NATURE REVIEWS DRUG DISCOVERY 25,460 47.12
4 LANCET 195,553 44.002
5 NATURE BIOTECHNOLOGY 48,650 43.113
6 NATURE REVIEWS IMMUNOLOGY 31,545 39.416
7 NATURE MATERIALS 72,306 38.891
8 NATURE REVIEWS MOLECULAR CELL BIOLOGY 36,784 38.602
9 NATURE 627,846 ... 阅读全帖 |
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o*******a
发帖数: 242 | 14 http://science.sciencemag.org/content/354/6316/1170
Abstract
The conversion of life-threatening viruses into live but avirulent vaccines
represents a revolution in vaccinology. In a proof-of-principle study, we
expanded the genetic code of the genome of influenza A virus via a
transgenic cell line containing orthogonal translation machinery. This
generated premature termination codon (PTC)–harboring viruses that exerted
full infectivity but were replication-incompetent in conventional cells.
Gen... 阅读全帖 |
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F*Q
发帖数: 3259 | 15 News from the National Academy of Sciences
May 2, 2017
FOR IMMEDIATE RELEASE
National Academy of Sciences Members and Foreign Associates Elected
The National Academy of Sciences announced today the election of 84 new
members and 21 foreign associates in recognition of their distinguished and
continuing achievements in original research.
Those elected today bring the total number of active members to 2,290 and
the total number of foreign associates to 475. Foreign associates are
nonvoting members... 阅读全帖 |
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s**********1
发帖数: 65 | 17 打扰了，受国内同学之托帮忙问问，患者病情危急，大家有什么建议。或者有知道什么
英文网站关于国际医疗救助的，
请告诉一下链接。十分感谢！
Case Report
Male, Chinese, 57y.
At the end of September in 2010, he had a bit pant and tired after a travel.
No obviously symptoms.
He then flight to Vancouver in Canada on October 5 and arrived there on
October 7. He had a toothache and
took some pills.
On October 8 or 9, he began to cough with sputum (yellow).
He started taking painkillers on October 12 when he flight to central Canada
which was colder than
Vancouver.
He pa... 阅读全帖 |
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e****0
发帖数: 678 | 18 • Exaggerated deep tendon reflexes can be seen in lock in syndrome.
• Non bleeding varices are managed with nonselective beta-
adrenergic antagonists, such as propranolol.
• Sclerotherapy, endoscopic band ligation, and surgery are
indicated after a patient has a first episode of variceal bleeding.
• TIPS is a last resort in variceal bleeding unresponsive to
medical and endoscopic intervention.
• Ischemic colitis
CT—thickening of eth bowel ... 阅读全帖 |
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j*******g
发帖数: 118 | 19 所有今年曾经接到翻译测试的ID，可以核实一下，
要求您翻译的第一段前一半是不是
INTRODUCTION— Gastric inflammatory disease can be broadly categorized into
gastritides and gastropathies. Gastritis is predominantly an inflammatory
process while gastropathy demonstrates minimal to no inflammation. The term
gastritis is used to denote inflammation associated mucosal injury.
要求您审阅纠错的第一段是不是
Given these data, the head of bed position should be individualized for each
patient [14].
• We recommend keeping the head in neutral alignment... 阅读全帖 |
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c****e
发帖数: 188 | 20 endoscopic mucosal resection of moderate dysplasia |
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yf
发帖数: 272 | 21 chemoradiation means chemo+concurrent radiation.
do 1 cycle chemo and 2 cycles chemo before after chemoradiation.
m means meter square, it's the surfacec area of a person. there is a formula
to calculate it based on the weight and height of a person. I cannot type
the m2 correctly.
Who is the oncologist of this patient? Why don't you ask questions to the
oncologist? The oncologist has the final say of what kind of chemo to use or
what is the whole plan. Capecitabine is only one of the chemo reg... 阅读全帖 |
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yf
发帖数: 272 | 22 chemoradiation means chemo+concurrent radiation.
do 1 cycle chemo and 2 cycles chemo before after chemoradiation.
m means meter square, it's the surfacec area of a person. there is a formula
to calculate it based on the weight and height of a person. I cannot type
the m2 correctly.
Who is the oncologist of this patient? Why don't you ask questions to the
oncologist? The oncologist has the final say of what kind of chemo to use or
what is the whole plan. Capecitabine is only one of the chemo reg... 阅读全帖 |
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r*****1
发帖数: 805 | 23 还有部分Abx总结，以前有经验贴分享USMLEforum的经典贴。但自己总结的东西容易记
住。希望给大家一点帮助。
P.S.: 顺着iamreallybad&斑竹思路，旁注中文为mnemonics.
Menigitis: G- use Ceftriaxone; bacilli use Gentamycin; DXM- in S. pneumonia
& unknown microb infection
G+ cocci: Vancomycin+Ceftriaxon
G+ bacilli (Listeria): Ampicillin + Gentamycin +
dexamethasone
G- cocci: Ceftriaxon
G- bacilli: Ceftriaxon+ Gentamycin
Fungus: Amphotericin+ Flucytosin IV-> Flucona... 阅读全帖 |
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z******8
发帖数: 844 | 24 ☆─────────────────────────────────────☆
rhcrc11 (Rebel) 于 (Thu May 15 00:13:42 2014, 美东) 提到:
Step3考场壮烈回来。第一天挺累人，最后一个block甚至做到有心无力状，看着题反应
不过来，导致来不及做完。非常没有信心能否通过。所以match之前先考Step3的童鞋，
需要考前调整生物钟，坚持锻炼，提高耐力。
MCQ没有资格说，CCS有一点小感受，拿出来分享下。大家请轻拍砖。
复习资料：
UW 52 online cases : 基础。最好复习早起找高手一起过一遍，尽快摸熟软件，进入
状态。第二遍找一位水平相近童鞋过一遍，不断总结protocol. 考前迅速做一遍，熟练
运用protocol.
UW 41 offline cases: 我当时和partner每个case仔细做一遍，虽然不像online有反馈
，但对练临床思维，补充protocol非常有帮助。最后考试与41 case有异曲同工之处，
遇到复杂case也不慌神。
CD 6 cases： 最后再熟悉下考试软件。与UW相近，但... 阅读全帖 |
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l******k
发帖数: 27533 | 25 同意楼上两位说的
5-FU是主要的chemo之一，抑制DNA合成的，治疗各种癌症
但毒副作用太大了，常见的严重SE: mucositis and hand and food syndrome
所以得要weight benefit and riks吧 |
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c*******s
发帖数: 399 | 26 This is a chronic diarrhea case, let me try the the red book algorithm
stool osmotic gap = 290-(Na+K)=162
with osmotic gap more then 50, positive fecal fat positive, it is likely
secondary to malabsorption/ osmotice cause.
with all available test result as above , should we further do
1. mucosal biospy?
2. check xvlose test for bacterial overgrowth
3. any evidence of cholestasis, cirrhosis. ERCP, ABD U
/S
people
would
... 阅读全帖 |
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