发帖数: 1 | 1 trying to switch to fast attack style. but it very hard to find red mutagen,
any suggestions on where to farm more? |
|
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m*******n
发帖数: 6660 | 3 他显然没有打到陶森特。
我mutagen从来没缺过，一般就是杀小怪掉的。 |
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s******9
发帖数: 283 | 4 NCBI protein database一般都有标注吧。
FEATURES Location/Qualifiers
source 1..503
/organism="Homo sapiens"
/db_xref="taxon:9606"
gene 1..503
/gene="TGFBR1"
/gene_synonym="ALK5"
/gene_synonym="SKR4"
Protein 1..503
/gene="TGFBR1"
/gene_synonym="ALK5"
/gene_synonym="SKR4"
... 阅读全帖 |
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W****r
发帖数: 835 | 5 http://en.wikipedia.org/wiki/Ethidium_bromide#Health_risks
The National Toxicology Program states it is nonmutagenic in rats and mice.[
10] These results are supported by a subchronic carcinogenicity study in
mice conducted at the university of Düsseldorf where also no mutagenic
effects could be detected.[11] Ethidium bromide (Homidium brand) use in
animals to treat trypanosome infection suggests that toxicity and
mutagenicity are not high. Studies have been conducted in animals to
evaluate EtBr... 阅读全帖 |
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u*******g
发帖数: 1808 | 6 几个解决方案（第三个我试过不work）：
1 花钱， app store 买id3mod2。不贵，有漂亮UI界面。
2 不花钱，
下载mutagen： http://code.google.com/p/mutagen/
安装mutagen （我用osx）
解压文件后到解压目录里面运行
./setup.py build
sudo ./setup.py install
到要转换的歌曲目录下
mid3iconv -e gbk *.mp3
搞定！（到itune里面重新倒入发现没有问题）
3 看到有人说在itune里面convert id3也可以，自己试验发现该完了没反应。
对于每天在命令行敲命令的人来说2是最好的选择。速度很快，而且不用花钱。
我花了半个小时搞定的，时间上面投资比买id3mod2贵多了。写下过程，希望帮助后人
节省时间。
按照我写的做，大概几分钟可以搞定。买id3mod2再安装，估计也要花那么多时间。 |
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S*******l
发帖数: 4637 | 7 提醒过你了。既然你不去看自己引用的文章，我就替你把文章结论贴上来吧：
An extraordinarily large volume of animal data has been compiled to evaluate
the carcinogenic potential of glyphosate. The expected normal biological
variability for spontaneous tumor formation is reflected across this
extensive data set (Tables 20–23). However, no specific neoplasm stands out
as a consequence of glyphosate exposures. While some individual studies may
note an increase in a specific neoplasm at the high dose, the pooled data
fail to identify any... 阅读全帖 |
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m********1
发帖数: 368 | 8 瘦肉精是一类动物用药，有数种药物被称为瘦肉精，例如莱克多巴胺（Ractopamine）及克伦特
罗（Clenbuterol）等。将瘦肉精添加于饲料中，可以增加动物的瘦肉量、减少饲料使用、使肉
品提早上市、降低成本。但因为考虑对人体会产生副作用，各国开放使用的标准不一。
瘦肉精是一类药物，而不是一种特定的物质，是指能够促进瘦肉生长的饲料添加剂。任何能够
促进瘦肉生长、抑制肥肉生长的物质都可以叫做“瘦肉精”。
目前，能够实现这种功能的物质是一类叫做β-促效剂（β-agonist）的药物，比如在中国造
成中毒的克伦特罗（clenbuterol）和美国允许使用的雷托巴胺（Ractopamine）。
瘦肉精在上海曾经引发了几百人的中毒事件。而在台湾，由于从美国进口的猪肉里含有瘦肉
精，几乎挑起一场政治争端。奇怪的是，在美国、加拿大、新西兰等国，瘦肉精这类物质的使用却
是合法的。在中国，通常所说的“瘦肉精”则是指克伦特罗。它曾经作为药物用于治疗支气管哮喘，
后由于其副作用太大而遭禁用。
其它这样类似药物还有沙丁胺醇（Salbutamol）和特布他林(Terbutaline)等，同样能起
到“瘦肉”... 阅读全帖 |
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t******g
发帖数: 17520 | 9 @@ mutation 的? resident evil 的那种？
牛逼的， 又有新东西要学。。。。。。。wiki 说 The first mutagens to be
identified were carcinogens
那就是说mutagens 是涵盖面最广的?
你说那种最安全 |
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q**x
发帖数: 1636 | 10 Material Safety Data Sheet
Sulfuric Acid
ACC# 22350
Section 1 - Chemical Product and Company Identification
MSDS Name: Sulfuric Acid
Catalog Numbers: S71211SC, S71826, S79200, S80213, S80213-1, A298 212,
A298212, A300 212, A300 225LB, A300 500, A300 612GAL, A300 700LB, A300212,
A300225LB, A300500, A300612GAL, A300700LB, A300C 212, A300C212, A300C212001,
A300C212002, A300C212003, A300C212004, A300C212005, A300C212006,
A300C212007, A300C212008, A300C212009, A300C212010, A300C212LC, A300FP 500,
A30... 阅读全帖 |
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q**x
发帖数: 1636 | 11 Material Safety Data Sheet
Sulfuric Acid
ACC# 22350
Section 1 - Chemical Product and Company Identification
MSDS Name: Sulfuric Acid
Catalog Numbers: S71211SC, S71826, S79200, S80213, S80213-1, A298 212,
A298212, A300 212, A300 225LB, A300 500, A300 612GAL, A300 700LB, A300212,
A300225LB, A300500, A300612GAL, A300700LB, A300C 212, A300C212, A300C212001,
A300C212002, A300C212003, A300C212004, A300C212005, A300C212006,
A300C212007, A300C212008, A300C212009, A300C212010, A300C212LC, A300FP 500,
A30... 阅读全帖 |
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e****r
发帖数: 581 | 12 You can use mutagen http://code.google.com/p/mutagen/ to convert the encodings of ID3 tags
make sure they are in unicode
typically id3 tags of downloaded mps from baidu are in gbk, gb18030 or
gb2312
iTunes uses unicode |
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q*****d
发帖数: 445 | 13 最近要做library, 使用这个实验室的方法进行易错PCR
（http://www.msg.ucsf.edu/agard/Protocols/PCR_Random_Mutagenesis.htm），酶
是NEB的Taq,但是效果一直不是很好，35 cycle PCR的浓度还是很低，30cycle特别的少
，请问
大家有什么方法提高产量吗？还是我的方法不够好，谢谢大家。
PCR Random Mutagenesis
Materials
1. Parental plasmid
2. Oligos surrounding region to be mutagenized
3. Error-prone PCR buffer (10x is 100mM Tris-HCl, pH8.3; 500mM KCl,
70mM MgCl2, 0.1% (w/v) gelatin.)
4. 10mM MnCl2 (stock) (make sure there is no brown coloring to
stock soln; if there is it means... 阅读全帖 |
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p*********g
发帖数: 9527 | 14 EtBr
Capable of binding to DNA, fluorescent dye, strong mutagen, but not THAT
strong. |
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C****g
发帖数: 2220 | 15 In conclusion, it was previously known that glyphosate consumption in water
above authorized limits may provoke hepatic and kidney failures (EPA). The
results of the study presented here clearly demonstrate that lower levels of
complete agricultural glyphosate herbicide formulations, at concentrations
well below officially set safety limits, induce severe hormone-dependent
mammary, hepatic and kidney disturbances. Similarly, disruption of
biosynthetic pathways that may result from overexpression... 阅读全帖 |
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y***l
发帖数: 1371 | 16 我想人家是被你的无知给气的了。
蒙三都的抗除草剂的转基因玉米，到你这变成了带除草剂的转基因玉米，居然能自己杀
死杂草了。如果你真能搞出自动杀杂草的转基因玉米，赶快申请专利，你就可以实现打
垮梦三度的目标了。
Herbicide resistant corns,
Corn varieties resistant to glyphosate herbicides (the original of which are
Roundup) were first commercialized in 1996 by Monsanto, and are known as "
Roundup Ready Corn".[1] Bayer CropScience developed "Liberty Link Corn' that
is resistant to glufosinate.[2] Pioneer Hi-Bred has develdoped and markets
corn hybrids with tolerance to imidazoline herbicides under the t... 阅读全帖 |
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a******e
发帖数: 94 | 17 新手100问又来啦。
要刷墙了，基本步骤已经考古了。
1. 据说通常色差大的需要先prime，我们准备把褐色墙刷成明快的淡黄色，这么说岂不
是要先prime?不prime即使是Sherwin-Williams一次coating都盖不住吧？
2. 家有baby，所以对用漆，地毯等特别注意VOC的问题，前两天看了版上一个帖子：
http://www.mitbbs.com/article_t/Living/32680485.html
说即使是0 voc的，也含微量PGE，容易导致孩子过敏哮喘什么的。 所以在犹豫该用哪
家的漆。
查了下这篇里说的那些牌子貌似home depot只有YOLO，说得很好，"has no VOCs, no
reproductive toxins, no phthlates, no mutagens, no formaldehyde, no
hazardous air pollutants, and no ozone-depleting compounds."
又考古到Sherwin-Williams的harmony貌似不错，0 VOC，达到室内空气质量标准green... 阅读全帖 |
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u********w
发帖数: 599 | 18 楼主你不要听一些人胡言乱语。有些帖子让人看了好笑。比如说，有人劝你不要告，因为
律师中有骗子，有些人说你告了没有医生愿意给你看病，还有人说“可能不完全“是医
生的责任。。。。 总之书读的脑子进水了，没一点common sense
我很快帮你google search 了一下 metronidazole&pregnancy。所有的网站都说甲硝锉
（Metronidazole）在孕妇中应用是controversial，开药必须慎之又慎，而且需要
careful monitoring。以下是FDA's official advice
Metronidazole Pregnancy Warnings
Metronidazole has been assigned to pregnancy category B by the FDA. Animal
studies failed to find evidence of fetotoxicity except when the drug was
administered intraperitoneally. There are no control... 阅读全帖 |
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h***a
发帖数: 312 | 19 来自主题: NextGeneration版 - GMO Do you know that a lot of GMO is present in baby food?
http://www.aaemonline.org/gmopost.html
https://www.youtube.com/watch?v=eeW5yUSqdhY
American academy of enviromental medicine
Genetically Modified Foods
According to the World Health Organization, Genetically Modified Organisms(
GMOs) are "organisms in which the genetic material (DNA) has been altered in
such a way that does not occur naturally."1 This technology is also
referred to as "genetic engineering", "biotechnology" or "recombinant D... 阅读全帖 |
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h***a
发帖数: 312 | 20 http://www.aaemonline.org/gmopost.html
American academy of enviromental medicine
Genetically Modified Foods
According to the World Health Organization, Genetically Modified Organisms(
GMOs) are "organisms in which the genetic material (DNA) has been altered in
such a way that does not occur naturally."1 This technology is also
referred to as "genetic engineering", "biotechnology" or "recombinant DNA
technology" and consists of randomly inserting genetic fragments of DNA from
one organism to ano... 阅读全帖 |
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t******8
发帖数: 2803 | 21 一直戴手套。现在开始在LD提醒下开始戴防毒面具，同时风扇强行通风。有些溶剂表示
没有"carcinogen"，但有"mutagen"。。。 |
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t******8
发帖数: 2803 | 22 光看carcinogen没用，还要看有没有mutagen。Hoppe 9就是没有前者有后者 |
|
|
发帖数: 1 | 24 can change color? wow... where? how?
i have shit loads of blue ones, many many green ones, but only has 1 lesser
and 2 normal red one. not enough to make even 1 greater red... |
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b*****e
发帖数: 53215 | 25 打到血与酒那个地图就有了 好像要先做一个变身任务
lesser |
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发帖数: 1 | 26 OK... mark down and try try later. |
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m*******n
发帖数: 6660 | 27 变异任务，收到夜奶的信，然后做完任务还要主线推到女伯爵奖了你个庄园，然后装修
房子，发现一个小黑屋…… |
|
发帖数: 1 | 28 kao... so much drama...
oh by the way... the other night i called a chicken just because curious, it
looks like the cut scene for papa is all the same.
kira metz, sasha, chicken, it's all the same, just different face.
veli disappointed... want my crowns back... |
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s**********1
发帖数: 305 | 29 because the mammary glands are developing in young women, which means active
cell divisions in those tissues. They are sensitive to mutagens, including
low dose radiation such as mammography or regular X rays. It is also why
higher estrogen is a risk factor, because it promotes cell division in those
tissues. Now the US has modified the recommended age when women should do
their first mammography. |
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s*****o
发帖数: 2490 | 30 http://pmep.cce.cornell.edu/profiles/herb-growthreg/fatty-alcoh
Glyphosate的急性毒性LD50>4000mg/kg体重，和食盐的毒性差不多，都属于低毒。也并
没有证据表明glyphosate有比较高的积累毒性。
“Tests on the biologically active ingredient in this formulation (
glyphosate) showed that glyphosate did not cause any mutagenic,
carcinogenic, teratogenic (birth defects), adverse reproductive changes
, or neurotoxic effects”
链接里是一个Glyphosate的chemical profile，里面毒理的东西讲的很清楚，还有参考
文献。
.. |
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h***a
发帖数: 312 | 31 http://www.aaemonline.org/gmopost.html
https://www.youtube.com/watch?v=eeW5yUSqdhY
American academy of enviromental medicine
Genetically Modified Foods
According to the World Health Organization, Genetically Modified Organisms(
GMOs) are "organisms in which the genetic material (DNA) has been altered in
such a way that does not occur naturally."1 This technology is also
referred to as "genetic engineering", "biotechnology" or "recombinant DNA
technology" and consists of randomly inserting geneti... 阅读全帖 |
|
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E*****m
发帖数: 25615 | 33
你東施效顰得很差勁。
進化論的突變有很多種， spontaneous mutation 是其中一種。
Four classes of mutations are (1) spontaneous mutations (molecular decay), (
2) mutations due to error prone replication by-pass of naturally occurring
DNA damage (also called error prone translesion synthesis), (3) errors
introduced during DNA repair, and (4) induced mutations caused by mutagens.
你問題根本不是是非題， 你要是問『spontaneous mutation 會不會造成演化』，答案
是『會』
要是問『進化論的突變是不是只有 spontaneous mutation}』，答案是『不是』，因為
至少還有其他三種。
該你回答。 （嚴格來說，是看你怎樣繼續閃躲不回答） |
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E*****m
发帖数: 25615 | 34 查了下 wiki, 跟我想的差不多， mutation 有許多種
http://en.wikipedia.org/wiki/Mutation#Causes
Four classes of mutations are (1) spontaneous mutations (molecular decay), (
2) mutations due to error prone replication by-pass of naturally occurring
DNA damage (also called error prone translesion synthesis), (3) errors
introduced during DNA repair, and (4) induced mutations caused by mutagens.
Spontaneous mutation 是四種裡的一種， 沒人說只有 Spontaneous mutation 才能造
成evolution 吧？
如果教徒硬要說這些 mutation 都是神幹的， 那也可以， 就是沒證據罷了， 還是與
理論相... 阅读全帖 |
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发帖数: 1 | 35 请诸位帮忙下载一篇文章
我只能看到abstract,本人email:
[email protected]/* */
Degradation of the natural mutagenic compound safrole in spices by cooking
and irradiation
不胜感谢 |
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e****r
发帖数: 581 | 36 u need to convert mp3 tags from gb2312 to utf-8
try easytag(with gui) or mutagen(cli) |
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a***e
发帖数: 1010 | 37 The most important role of homologous recombination is to repair DNA damages
which are caused by various reasons, such as mutagens.
An indirect role of homologous recombination is to increase the diversity
and variability of the genome of species, which brings a lot of evolutional
advantages. |
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T**********t
发帖数: 1604 | 38 Google一下很容易就知道了啊：
Acetone is not currently regarded as a carcinogen, a mutagenic chemical or a
concern for chronic neurotoxicity effects. |
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s******y
发帖数: 28562 | 39 谢谢你贴出这个文章。非常有用的一个资料。比这个系列里很多空对空的争执
有用。
我得指出的一点是： EPA的这个资料里明确说了：他们只测了短期的毒性，
但是他们认为长期的毒性应该是由短期的毒性积累后引起的。所以在短期没有毒性
的情况下就没有测长期毒性的必要。
The acute oral toxicity data submitted support the determination that the
Cry1Ab protein is non-toxic to humans. When proteins are toxic, they are
known to act via acute mechanisms and at very low dose levels (Sjoblad, et
al.1992). Since no effects were shown to be caused by the plant-incorporated
protectants, even at relatively high dose levels, the Cry1Ab delta-
endo... 阅读全帖 |
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e*r
发帖数: 103 | 40 what kind of mutagen did you use? Why do you think there is something
changed in methylation? |
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M*****n
发帖数: 16729 | 41 this commetator is mean.
Piggybac is great, though transposons are never as unbiased as chemical
mutagens. |
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a***e
发帖数: 1010 | 42 computer mutagenize, then chemical synthesize. |
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y******8
发帖数: 1764 | 43 很简单，候选人的学术成就是否在其领域代表了最高水平。
至于学术成就如何评价，以前是在国内最高就行。现在与国际接轨，科学院应该更强调
原创性，工程院更强调。。。（我不是搞工科的，觉得应该更着重解决实际的问题，和
问题的难度。）
袁隆平的例子很特殊，找到野生水稻雄性不育株是个非常非常辛苦的活，这让一切杂交
水稻的理论得以实现。我不知道版上的有谁愿意大热天去野外做纯天然(没有mutagen,
非实验室株系)的screen.如果诺贝尔奖有农学的话，袁隆平也是名至时归。何况他也拿
到了Wolf Prize, 已经是最高荣誉了。
最后想说一句，院士也好，诺奖也好，对于大部分人来说，是别人的荣誉。对于极少部
分人来说，是一时的荣誉。大家的生活远比这丰富多彩。 |
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n********k
发帖数: 2818 | 44 this discussion is getting a bit interesting:
I think both you and Oct4/p are correct...they are more right though:))...
The direction is controlled by nurture but the mutation is accidental by
nature...Population genetic---it is more nurture, but at molecular level, it
is nature/mutation.
However, what I like to raise now, whether your other point could bear
some true elements or Lamark is not entirely wrong...is inherited
epigenetics true and whether that could confer/accelerate mutations...wh... 阅读全帖 |
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s*****o
发帖数: 2490 | 45 你提供的这片文章太烂了，居然用1-10000ppm的剂量。1ppm glyphosate大概是6uM，对
于生理浓度来说是非常非常高的了，10000ppm更是没有可能在正常生理情况下出现。可
以这么说就算是10000ppm的糖都可能对细胞产生很强的伤害。我看到文章里提到1ppm是
人尿液中的浓度，我仔细找了找，文中并没有提出reference，有一个地方模模糊糊的
有一个引用，查了一下引文，人家测的在施用农药的工人以及家人中 glyphosate在尿中的浓度是ppb级别的，最高也只有233ppb，平均是3ppb，比1ppm差的
远了去了。就这个烂文章居然也能发表~~
再给你一个科普，关于glyphosate的毒理学性质的
http://pmep.cce.cornell.edu/profiles/herb-growthreg/fatty-alcoh
Glyphosate的急性毒性LD50>4000mg/kg体重，和食盐的毒性差不多，都属于低毒。也并
没有证据表明glyphosate有比较高的积累毒性。
“Tests on the biologically active ingredien... 阅读全帖 |
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l**********1
发帖数: 5204 | 46 linker-based PCR
plus
iMapper soft:
//www.ncbi.nlm.nih.gov/pubmed/18974167
//geocachingsoftware.com/imapper.html
more details
please to to E-Book
Chapter 2:
its link:
FTP://ftp.sanger.ac.uk/pub4/theses/kong/chapter2.pdf
citation:
>Based on these expectations, a web-based server called iMapper (Insertional
Mutagenesis Mapping and Analysis Tool) was developed for the efficient
analysis of insertion site sequence reads against vertebrate and
invertebrate Ensembl genomes. Taking linker-based PCR se... 阅读全帖 |
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l**********1
发帖数: 5204 | 47 cited,
>Both zinc finger nucleases (ZFNs) and TAL effector nucleases (TALENs)
>can be used to mutagenize genomes at specific loci, but
these systems require two different DNA binding proteins
flanking a sequence of interest, each with a C-terminal
FokI nuclease module. As a result these methods have not
been widely adopted by the plant research community.
from
>Belhaj K et al., (2013).
>Plant genome editing made easy: targeted mutagenesis in model and crop
plants using the CRISPR/Cas >system.
>P... 阅读全帖 |
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n******e
发帖数: 89 | 48 EB应该是被妖魔化了，看看NIH TOXICOLOGY DATABASE其实在动物中都算不上是
mutagen。现在的新染料未必就比EB安全。 |
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发帖数: 1 | 49 请诸位帮忙下载一篇文章
我只能看到abstract,本人email:
[email protected]/* */
Degradation of the natural mutagenic compound safrole in spices by cooking
and irradiation
不胜感谢 |
|
