w********r
发帖数: 1431 | 1 你的primary myocyte是说satellite cell么？
如果是的话，我用lipoRNAiMax往primary myoblast里转过siRNA，效率还可以，我做
western看过。
转plasmid确实是进不去，我都是用Adenovirus。
不过看业内大牛的paper也有往里面裸转质粒的，具体用的啥试剂记不清楚了，你可以
去查查Michael Rudnicki的paper. |
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F****n
发帖数: 438 | 2 【 以下文字转载自 Medicalpractice 讨论区 】
发信人: FeiLun (Is Horrible), 信区: Medicalpractice
标 题: 几篇期刊文章
发信站: BBS 未名空间站 (Sat Jul 12 22:36:30 2014, 美东)
那位大侠可以帮组查一下这几篇的全文吗？叩谢。
请发：y******[email protected].
I.
Role of small-conductance calcium-activated potassium channels in atrial
electrophysiology and fibrillation in the dog.
Qi XY1, Diness JG, Brundel BJ, Zhou XB, Naud P, Wu CT, Huang H, Harada M,
Aflaki M, Dobrev D, Grunnet M, Nattel S.
Author information
Abstract
BACKGROUND:
Recent evidence points to functional... 阅读全帖 |
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F****n
发帖数: 438 | 3 【 以下文字转载自 Medicalpractice 讨论区 】
发信人: FeiLun (Is Horrible), 信区: Medicalpractice
标 题: 几篇期刊文章
发信站: BBS 未名空间站 (Sat Jul 12 22:36:30 2014, 美东)
那位大侠可以帮组查一下这几篇的全文吗？叩谢。
请发：y******[email protected].
I.
Role of small-conductance calcium-activated potassium channels in atrial
electrophysiology and fibrillation in the dog.
Qi XY1, Diness JG, Brundel BJ, Zhou XB, Naud P, Wu CT, Huang H, Harada M,
Aflaki M, Dobrev D, Grunnet M, Nattel S.
Author information
Abstract
BACKGROUND:
Recent evidence points to functional... 阅读全帖 |
|
F****n
发帖数: 438 | 4 来自主题: Medicalpractice版 - 几篇期刊文章 那位大侠可以帮组查一下这几篇的全文吗？叩谢。
请发：y******[email protected].
I.
Role of small-conductance calcium-activated potassium channels in atrial
electrophysiology and fibrillation in the dog.
Qi XY1, Diness JG, Brundel BJ, Zhou XB, Naud P, Wu CT, Huang H, Harada M,
Aflaki M, Dobrev D, Grunnet M, Nattel S.
Author information
Abstract
BACKGROUND:
Recent evidence points to functional Ca2⁺-dependent K⁺ (SK)
channels in the heart that may govern atrial fibrillation (AF) risk, but the
underlying mechanisms are... 阅读全帖 |
|
发帖数: 1 | 5 【Filariasis
From Wikipedia, the free encyclopedia
Filariasis
Life cycle of Wuchereria bancrofti, a parasite that causes filariasis
Classification and external resources
Specialty Infectious disease
ICD-10 B74
ICD-9-CM 125.0-125.9
Patient UK Filariasis
MeSH D005368
[edit on Wikidata]
Filariasis is a parasitic disease caused by an infection with roundworms of
the Filarioidea type.[1] These are spread by blood-feeding black flies and
mosquitoes. This disease belongs to the group of d... 阅读全帖 |
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发帖数: 1 | 6 12月27日 为什么我要替中国辩护
我要替中国辩护，并不是因为我是一个中国人，而是因为我知道中国就和这世界上绝大
数的国家一样，虽然有很多丑陋的现象，但是他们是受害者。就
象这个犯罪集团里很多的人一样，他们来自各种种族，他们可能充当了工具，但他们不
是制造这一切，谋划这一切的人。虽然经常会暴露中国产品出现问题。但要知道中国是
世界工厂，他们只是打工者，他们只是按照各种雇主提供的配方来生产，他们并不知道
他们会产生怎样的后果。或者这些工人知道后果，但他们只是奴隶，他们又如何去改变
这一切？ 所以，当我的调查不断往前推进的时候，不可避免地会涉及各国的企业，但
这并不表明是针对这个国家或这个国家的人民，而是针对一种社会现实和丑陋现象。就
拿菲律宾来说，菲律宾是一个犯罪猖獗的国家，有很多犯罪分子，但是菲律宾人民却是
这个集团的受害者，他们／她们被绑架，沦为了牺牲品，人体实验对象。一个日本人，
强奸上千名菲律宾女孩，却没有受到惩罚。以前我也提到过，有20个国家的名单，这20
个国家的人民其实都是受害者。现在他们把矛头对准中国，或许仅仅是因为我揭示了他
们图害世界人民的内幕，而我是一个中国人的缘... 阅读全帖 |
|
发帖数: 1 | 7 12月27日 为什么我要替中国辩护
我要替中国辩护，并不是因为我是一个中国人，而是因为我知道中国就和这世界上绝大
数的国家一样，虽然有很多丑陋的现象，但是他们是受害者。就
象这个犯罪集团里很多的人一样，他们来自各种种族，他们可能充当了工具，但他们不
是制造这一切，谋划这一切的人。虽然经常会暴露中国产品出现问题。但要知道中国是
世界工厂，他们只是打工者，他们只是按照各种雇主提供的配方来生产，他们并不知道
他们会产生怎样的后果。或者这些工人知道后果，但他们只是奴隶，他们又如何去改变
这一切？ 所以，当我的调查不断往前推进的时候，不可避免地会涉及各国的企业，但
这并不表明是针对这个国家或这个国家的人民，而是针对一种社会现实和丑陋现象。就
拿菲律宾来说，菲律宾是一个犯罪猖獗的国家，有很多犯罪分子，但是菲律宾人民却是
这个集团的受害者，他们／她们被绑架，沦为了牺牲品，人体实验对象。一个日本人，
强奸上千名菲律宾女孩，却没有受到惩罚。以前我也提到过，有20个国家的名单，这20
个国家的人民其实都是受害者。现在他们把矛头对准中国，或许仅仅是因为我揭示了他
们图害世界人民的内幕，而我是一个中国人的缘... 阅读全帖 |
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发帖数: 1 | 8 12月28日 仔细阅读以下文章
或许这些文章可以告诉你们孩子被伤害病毒的答案。
Astellas Pharma 应该和这些人体实验有莫大的关系！
Founded 2005; 11 years ago
Headquarters 2-5-1, Nihonbashi-Honcho, Chūō-ku, Tokyo 103-8411, Japan
Key people Yoshihiko Katakana
(President and CEO)
Filariasis
From Wikipedia, the free encyclopedia
Filariasis
Life cycle of Wuchereria bancrofti, a parasite that causes filariasis
Classification and external resources
Specialty Infectious disease
ICD-10 B74
ICD-9-CM 125.0-125.9
Patient UK Filariasis
MeSH ... 阅读全帖 |
|
发帖数: 1 | 9 12月27日 为什么我要替中国辩护
我要替中国辩护，并不是因为我是一个中国人，而是因为我知道中国就和这世界上绝大
数的国家一样，虽然有很多丑陋的现象，但是他们是受害者。就
象这个犯罪集团里很多的人一样，他们来自各种种族，他们可能充当了工具，但他们不
是制造这一切，谋划这一切的人。虽然经常会暴露中国产品出现问题。但要知道中国是
世界工厂，他们只是打工者，他们只是按照各种雇主提供的配方来生产，他们并不知道
他们会产生怎样的后果。或者这些工人知道后果，但他们只是奴隶，他们又如何去改变
这一切？ 所以，当我的调查不断往前推进的时候，不可避免地会涉及各国的企业，但
这并不表明是针对这个国家或这个国家的人民，而是针对一种社会现实和丑陋现象。就
拿菲律宾来说，菲律宾是一个犯罪猖獗的国家，有很多犯罪分子，但是菲律宾人民却是
这个集团的受害者，他们／她们被绑架，沦为了牺牲品，人体实验对象。一个日本人，
强奸上千名菲律宾女孩，却没有受到惩罚。以前我也提到过，有20个国家的名单，这20
个国家的人民其实都是受害者。现在他们把矛头对准中国，或许仅仅是因为我揭示了他
们图害世界人民的内幕，而我是一个中国人的缘... 阅读全帖 |
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S******9
发帖数: 2837 | 10 有几道题目是关于运动training以后肌肉改变。
大概有：
1.举重训练以后股四头肌的变化
2.长跑，尤其是马拉松训练以后，腓肠肌的变化
3.短跑训练以后腓肠肌的变化
下面是变化的指标：
number of of myocyte
amount of actin per myocyte
number of mitochondria
thanks |
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f****o
发帖数: 2770 | 11 GENERAL PRINCIPLES
The first step in managing chronic HF is to determine the etiology or
precipitating factors. Treatment of underlying disorders (e.g., anemia,
hyperthyroidism) may obviate the need for treatment of HF.
Nonpharmacologic interventions include cardiac rehabilitation and
restriction of fluid intake (maximum 2 L/day from all sources) and dietary
sodium (approximately 1.5 to 2 g of sodium per day).
Stage A: The emphasis is on identifying and modifying risk factors to
prevent developm... 阅读全帖 |
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W*****s
发帖数: 684 | 12 【 以下文字转载自 Chemistry 讨论区 】
发信人: Whoiwas (Who I am?), 信区: Chemistry
标 题: Help me with this paper. Thanks!
关键字: MLHF
发信站: BBS 未名空间站 (Tue Jun 7 23:43:16 2011, 美东)
Nanomedicine
Volume 2, Issue 6, December 2007, Pages 831-846
Delivery of nano-objects to functional sub-domains of healthy and failing
cardiac myocytes
Thanks! |
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W*****s
发帖数: 684 | 13 【 以下文字转载自 Chemistry 讨论区 】
发信人: Whoiwas (Who I am?), 信区: Chemistry
标 题: Help me with this paper. Thanks!
关键字: MLHF
发信站: BBS 未名空间站 (Tue Jun 7 23:43:16 2011, 美东)
Nanomedicine
Volume 2, Issue 6, December 2007, Pages 831-846
Delivery of nano-objects to functional sub-domains of healthy and failing
cardiac myocytes
Thanks! |
|
m*******6
发帖数: 314 | 14 Position Title: Postdoctoral Research Trainee
Company Name: University of Tennessee Health Science Center
Job Function: Postdoc
Entry Level: No
Location(s): Memphis, Tennessee, 38163, United States
Posted: June 23, 2014
Job Type: Full-Time
Min Education: Ph.D.
Min Experience: 1-2 Years
Required Travel: None
Contact Person: Adebowale Adebiyi
Email Address: a******[email protected]
Job Description
A postdoctoral position for a trained patch-clamp electrophysiologist... 阅读全帖 |
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a*******s
发帖数: 1539 | 15 Fast VG, Ideker RE. Simultaneous optical mapping of transmembrane potential
and intracellular calcium in myocyte cultures. J Cardiovasc Electrophysiol.
2000
my email: h*****[email protected], thanks a lot |
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d******e
发帖数: 351 | 16 试了两次，真是油盐不进啊，转化效率约等于零。
各位高人有好用的PROTOCOL吗？ |
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A******y
发帖数: 2041 | 18 virus...using chemical agents for primary cells and lymphocytes don't expect
much. Now you know why big pharmas are dropping RNAi division. |
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m*p
发帖数: 226 | 21 Fibroblast are everywhere or in every organ, skin, heart, liver, etc.
Fibroblast are a type of mesenchymal cell, which are usually derived from
the epithelial-mesenchymal transition process. It seems fibroblast can be
derived from ectoderm, mesoderm, and endoderm. Fibroblast synthesize ECM and
collagen, and are important for organ structure (my personal two cents).
Myo-blast are myocyte progenitors. |
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s******y
发帖数: 220 | 22 练习分细胞有段时间了，但是很奇怪的是有时候分出来的细胞看上去形态很好，可是给
刺激时候，细胞却不会contract，有经验的同学请指教，为什么会这样呢？
谢谢。 |
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W*****s
发帖数: 684 | 24 Nanomedicine
Volume 2, Issue 6, December 2007, Pages 831-846
Delivery of nano-objects to functional sub-domains of healthy and failing
cardiac myocytes
Thanks! |
|
M********3
发帖数: 269 | 25 解释的很好。 多谢！
下面是我查的解释：
However, in the heart, it causes myocytes to become hyperexcitable. This is
due to two independent effects that may lead to aberrant cardiac conduction
and subsequent arrhythmia: 1) there are more inactivated sodium (Na)
channels available to fire, and 2) the overall potassium permeability of the
ventricle is reduced (perhaps by the loss of a direct effect of
extracellular potassium on some of the potassium channels), which can delay
ventricular repolarization. |
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M********3
发帖数: 269 | 26 解释的很好。 多谢！
下面是我查的解释：
However, in the heart, it causes myocytes to become hyperexcitable. This is
due to two independent effects that may lead to aberrant cardiac conduction
and subsequent arrhythmia: 1) there are more inactivated sodium (Na)
channels available to fire, and 2) the overall potassium permeability of the
ventricle is reduced (perhaps by the loss of a direct effect of
extracellular potassium on some of the potassium channels), which can delay
ventricular repolarization. |
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h***a
发帖数: 312 | 27 Q.
A 5-y-o girl is brought to the physician by her parents for an initial
physical examination. They recently moved to town, and they failed to bring
any medical records. They state that the child was "behind" in most of her
developmental milestones, and that her teeth erupted "late." During the last
3 years, she has sustained fractures of both upper extremities as well as
her left lower extremity. The physician notes a distinct nasal quality to
the child's voice. Her height is at the 10th p... 阅读全帖 |
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w******n
发帖数: 61 | 28 19. A 60-year-old woman is brought to the emergency department by her
husband because of chest pain that began 3 hours ago. She is dyspneic and
diaphoretic, and she appears anxious Her pulse is 110/rnin and regular,
respirations are 35'min, and blood pressure is 190/110 mm Hg An ECG shows an
acute myocardial infarction Winch of the following is the most likely cause
of this patient's pain?
A) Abnormalities of ion transport in myocytes
B) Accumulation of metabolic products in heart tissue
C) Incr... 阅读全帖 |
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h*******y
发帖数: 1220 | 29 did some search:
myocardial troponin can also be released in the setting of increased
membrane permeability (4). With increased membrane permeability, smaller
troponin fragments could be released into the systemic circulation. In this
setting, myocyte damage may not be permanent and necrosis does not occur. |
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w******y
发帖数: 4871 | 30 NOVEL THERAPIES
Gene therapy — The identification of the gene responsible for the
dystrophinopathies led to initial excitement that transfer of a functioning
dystrophin protein (whether by transplanted myoblast or direct genetic
manipulation) may provide substantial benefit to or even "cure" affected
patients. Although myoblast transfer has been attempted in humans, the
results have thus far not been encouraging [47]. However, in one patient who
received a bone marrow transplant for severe combi... 阅读全帖 |
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