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f*****5
发帖数: 3 | 2 只要打http://ncbi.nlm.nih.gov 就可以直接联上；
而打ncbi.nlm.nih.gov,就总是联到weblogin,我以前学校的登陆页面(离开很久了，密
码什么早不能用了)。
cookie 什么的应该都清除了，搞不懂，请大拿指教，谢了！ |
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d****m
发帖数: 3 | 5 NCBI Reference Sequences
Definition: The NCBI Reference Sequence project (RefSeq) provides reference
sequence standards for the naturally occurring molecules of the central dogm
a, from chromosomes to mRNAs to proteins. Toward this goal, intermediate lar
ger genomic regions, instantiated as accessions of the format NG_123456 (gen
omic sequence with curated annotation) or NT_123456 (computed assembly and a
nnotation) are also produced. RefSeq standards provide a foundation for the
functional anno |
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v***a
发帖数: 1242 | 7 在NCBI上查到蛋白的mRNA序列，要设计引物以便引入Expression Vector中。
但这个蛋白我不需要全长，而是仅需要它的一部分，还得在比较精确的位置停止PCR的
反应，所以我想在要它停的地方引入一个TGA的stop codon。我平时设计anti-sense的
引物都是根据在NCBI上查到的mRNA序列从3'开始reverse读出一段作为引物，要引入此
stop codo的话，我有点想不通该怎么引入了，是要把TGA反向变为ACT加在平时正常的
引物后吗？
还有一个白痴问题，为什么我查到的mRNA序列会和我在别人paper上看到的cDNA序列一
模一样？
谢谢大家。 |
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m*********7
发帖数: 606 | 10 从个人经验说吧，我认为目前对于isoform的归纳总结极少，历史上遗留下来的问题极
多，而且很多人不在乎。你所提到的那些问题如果想得到答案，必须自己去下功夫挖了。
当年我做一个基因，文献历史就已经很传奇了。最早从cDNA库调出的两个基因，其中一
个在九年后被人发现有严重测序错误，另一个11年后被发现是从rat cDNA库里调出来的
（最早被当做人的基因发表）。我根据那11年后宣称是人的基因去做克隆，死活做不出
来。最后才发现这是一个只在个别组织里表达的特定isoform，不是普遍表型。直到今
天NCBI里的记录都是不正确的。
后来做几个别的基因，都或多或少有类似问题。搞得我现在每次都是在NCBI里把所有相
关记录都翻出来仔仔细细看，并且恨不得每个基因都自己做一遍5'-RACE和3'-RACE。 |
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c*******d
发帖数: 192 | 13 Nature. 2010 May 27;465(7297):473-7.
Sequence data has been submitted to the NCBI Short Read Archive under
accession number SRA012097. Microarray data has been submitted to the NCBI
Gene Expression Omnibus under accession number GSE20585. |
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b**********t
发帖数: 366 | 15 1.
Journal: Current drug targets
Title: Vascular Endothelial Growth Factor as an Anti-Angiogenic Target for
Cancer Therapy
Author: Niu, etc
Year 2010, Volume 11, Issue 8, Page 1000-1017
Link:
http://www.ncbi.nlm.nih.gov/pubmed/20426765
2.
Journal: Expert opinion on biological therapy
Title: Oligosaccharides as anti-angiogenic agents
Author: Cole, etc
Year 2008, Volume 8, Issue 3, Page 351-362
Link:
http://www.ncbi.nlm.nih.gov/pubmed/18294105
My email: b********[email protected]
Thanks a lot! :) |
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z***k
发帖数: 33 | 25 挂一漏万，抛砖引玉。
Gilenya/FTY720：免疫抑制剂。临床用于治疗自身免疫性疾病（如硬皮病）。最早由日
本人提取于冬虫夏草。
On September 22, 2010, fingolimod became the first oral disease-modifying
drug approved by the Food and Drug Administration to reduce relapses and
delay disability progression in patients with relapsing forms of multiple
sclerosis. In 2010 and 2011 it was approved for treating multiple sclerosis
in USA/Russia and Europe/Canada/Australia respectively. It is derived from
the myriocin (ISP-1) metabolite of the fungus Isaria sinclai... 阅读全帖 |
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s**u
发帖数: 9035 | 28 1.
Curr Med Chem. 2010;17(36):4427-32.
miRNA: small molecules as potential novel biomarkers in cancer.
Shah AA, Leidinger P, Blin N, Meese E.
Division of Molecular Genetics, Institute of Human Genetics, University of T
übingen, Germany.
http://www.ncbi.nlm.nih.gov/pubmed/21062260
2.
Adv Cancer Res. 2010;108:113-57.
microRNAs in cancer: from bench to bedside.
Cortez MA, Ivan C, Zhou P, Wu X, Ivan M, Calin GA.
Department of Experimental Therapeutics, The University of Texas MD Anderson
Cancer Cent... 阅读全帖 |
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s**u
发帖数: 9035 | 29 1.
Curr Med Chem. 2010;17(36):4427-32.
miRNA: small molecules as potential novel biomarkers in cancer.
Shah AA, Leidinger P, Blin N, Meese E.
Division of Molecular Genetics, Institute of Human Genetics, University of T
übingen, Germany.
http://www.ncbi.nlm.nih.gov/pubmed/21062260
2.
Adv Cancer Res. 2010;108:113-57.
microRNAs in cancer: from bench to bedside.
Cortez MA, Ivan C, Zhou P, Wu X, Ivan M, Calin GA.
Department of Experimental Therapeutics, The University of Texas MD Anderson
Cancer Cent... 阅读全帖 |
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l**********1
发帖数: 5204 | 30 在欧美富不过三代, Thomas R. Cech 诺奖光环也不可能传三代:就看二代徒弟Ming Lei
在美都是只mentor华人博士生和千老的份上, 缺乏脑力潜能资源多样性吧, 海归后清后
更没诺奖光环戏?
www.biochem.med.umich.edu/?q=ming_lei
Ps: 指Ming Lei 作为通讯作者的第一作者Last name 有非华文拼音的吗?
www.ncbi.nlm.nih.gov/pubmed?term=lei+m[au]+telomere
Ming Lei 作为第2作者 通讯作者为他人的不算 i.e.
www.ncbi.nlm.nih.gov/pmc/articles/PMC2490755/?tool=pubmed |
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l**********1
发帖数: 5204 | 32 RE
1.这回你Microtus (田鼠啦啦啦) 误解了,
>关于你的第一个问题，pi和pd，学生的关系，你的意思是不是如果pd，学生过的好一
些，
----------------'
我是说12年大选如果共和党的总统上台把NIH的90% R01/K99 Cut了以后, 让那些还米美
国绿卡的破四刀咋办? 是跳和谐Shi YG or RY PI那里呢还是回其她大陆的国家那里去
当pi奴隶啊? 原始丛林社会中不讲究EQ 只重视CFQ=Catch Food Q 当然需要IQ去竞
争获得的.
*******************
2
当然当然好的收入容易出学术成果但是如果考虑annual收入多少USD/ 比裴多非的生
命,爱情, 自由还要重的话, 伽罗瓦还会書出伽罗瓦群論吗? 阿贝尔还会書出阿贝尔群
吗? 今天生物工程数学领域还用到这些群論Multi Scale Complex systems biology:
please refer
Gerhard Werner
Fractals in the Nervous System: Conceptual Implications fo... 阅读全帖 |
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l**********1
发帖数: 5204 | 33 So what's up next cutting edge MS proteomics method?
ZZ
Why nanoLiters?
Nanoliter Cool Wave®
exciting, inexpensive, sample handling platform & technology.
Booth 17 Improve sensitivity of MALDI, DART, DESI, SIMS, LDI 10 to 100x
LITERALLY!
Also, manipulate and spatially, temporally concentrate samples for TLC, MS,
IR, NMR, PCR; electrophoresis, fluorescence, crystallization & more.
Dispense viscous liquids non-touch (glycerol, blood, serum, ionic liqs.,
glues) for forensics, defense, medical,... 阅读全帖 |
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l**********1
发帖数: 5204 | 37 RT
by
Postnikov EB, Verveyko DV, Verisokin AY
Simple model for temperature control of glycolytic oscillations.
Phys Rev E Stat Nonlin Soft Matter Phys. 2011 Jun;83(6 Pt 1):062901. Epub
2011 Jun 16.
ncbi link:
http://www.ncbi.nlm.nih.gov/pubmed/21797427
my mail address:
Thanks a million in advance! |
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z**********8
发帖数: 766 | 39 1) http://www.ncbi.nlm.nih.gov/pubmed/22082758
Semin Oncol. 2011 Dec;38(6):724-33.
MicroRNAs in the pathogenesis of cancer.
Lovat F, Valeri N, Croce CM.
Molecular Virology Immunology & Medical Genetics, The Ohio State University
Comprehensive Cancer Center, Columbus, OH 43210, USA.
2) http://www.ncbi.nlm.nih.gov/pubmed/22082760
Semin Oncol. 2011 Dec;38(6):743-51.
MicroRNAs in mutagenesis, genomic instability, and DNA repair.
Landau DA, Slack FJ.
Department of Hematology, Yale University School o... 阅读全帖 |
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l**********1
发帖数: 5204 | 40 So Plos Biology permit three Co-first-authoship even not post-genome/trans
proteomics report of one major model species likes below 2002 Science
htp://www.ncbi.nlm.nih.gov/pubmed/11935017
or 2009 PNAS
htp://www.ncbi.nlm.nih.gov/pubmed/19372371
jinglingzi (JingLingZi) 的大作中提到:
htp://www.mitbbs.com/article_t/Biology/31624531.html |
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l**********1
发帖数: 5204 | 41 De rien.
Plus another one paper:
htp://www.ncbi.nlm.nih.gov/pubmed/22203841
>Grey scale images are generated, reproducing the fluo- rescence intensity
in different tissue components (Figure 1). The estimated resolution is 0.5
μm in lateral direction and 1- 2 μm in the axial direction
full text link:
htp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3235701/pdf/DRP2012-810749.pdf
if LZ can not recognize 200 μm scale labeled GFP particle color signal just try at least 30 μm or more sensitive
likes above ... 阅读全帖 |
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l**********1
发帖数: 5204 | 42 if transfer to mice
pls go to
htp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2791161/
its Fig. 3B
and if transfer to cell lines
htp://www.ncbi.nlm.nih.gov/pubmed/22134125 |
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l**********1
发帖数: 5204 | 43 Pls go to
httq://www.ncbi.nlm.nih.gov/pubmed/21926998
NB: http now is "httq" from some bug might happened while using direct URL
address here.
if FAQ (Just F A Q) 的 target scale is below 15nm i.e. virus likes HSV
now your only choice is SEM not fluorescence microscopy.
pls refer
2011 paper:
httq://www.ncbi.nlm.nih.gov/pubmed/21345968
Ps:
这里也是现炒现卖 没有楼上的提示
还得不到
科普 偶本人 和whom want to know sth about size scale:
30 nm Small virus (Picornaviruses)
httq://en.wikibooks.org/wiki/Cell_Biology/Introducti... 阅读全帖 |
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l**********1
发帖数: 5204 | 44 not only Biology but Chemistry:
please go to
//www.ncbi.nlm.nih.gov/pubmed/20929839
or
//www.ncbi.nlm.nih.gov/pubmed/21310963 |
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l**********1
发帖数: 5204 | 46 LZ please try below (I) to (IV) four strands overlap PCR
your target 7kp=2kp(I)+2kp(II)+2kp(III)+1kp(IV)
if you did not know downstream any conservative sequence information
your 3' RACE can use ligation-anchored PCR
就是用内切酶
//en.wikipedia.org/wiki/Restriction_enzyme
对应的人工引物锚定long mRNA 的3'端 做人工锚定引物
i.e. by NotI it should be inserted to 3'端 5'GC---GGCCGC
from
//en.wikipedia.org/wiki/Restriction_enzyme
Enzyme Source Recognition Sequence Cut
NotI Nocardia otitidis
5'GCGGCCGC
3'CGCCGG... 阅读全帖 |
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l**********1
发帖数: 5204 | 47 让你老板买个半自动或全自动工作站做Y-2-H 不就得拉
或借用同校里的其它单元的那设备
没听说过欧美高通量Y-2-H 筛选用一双手做的
半自动工作站 96 well
please below link:
//www.tecan.com/platform/content/element/5135/Tecan_Journal_09_01.pdf
for one
France developed a semi- automated yeast two-hybrid system with the Te-
MOTM 96 Multichannel Pipetting Option from Tecan, halving the time taken to
obtain reproducible results from six to three days.
if try Y-1-H
全自动工作站 384 well
please refer last Oct. Nature Methods two papers:
//www.ncbi.nlm.nih.gov/pubmed/22037705
/... 阅读全帖 |
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l**********1
发帖数: 5204 | 48 Please try refer
if could do 2D/3D QSAR Quantitative structure–activity relationship tool to
your N protein with B
papers link:
//www.ncbi.nlm.nih.gov/pmc/articles/PMC3141047/
or
//www.ncbi.nlm.nih.gov/pubmed/20726601
----
other approach:
Do N X-ray 3D structure analysis if PDB bank without its record
Normal Protocol process:
please go to
//uit.no/publikum/prosjekter/prosjekt?p_document_id=228973 (QSAR)
For information services and faciities, please follow the links below.
SP1 Protein productio... 阅读全帖 |
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l**********1
发帖数: 5204 | 49 COLD-PCR then Agarose Gel electrophoresis
Abstract
The detection of low-abundance DNA variants or mutations is of particular interest to medical diagnostics,
individualized patient treatment and cancer prognosis; however, detection sensitivity for low-abundance
variants is a pronounced limitation of most currently available molecular assays. We have recently
developed coamplification at lower denaturation temperature-PCR (COLD-PCR) to resolve this limitation.
//www.ncbi.nlm.nih.gov/pmc/articles... 阅读全帖 |
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