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a*****x
发帖数: 901 | 3 是我猜测应该不行，因为印象中有XXY的人但是没有XY的oocyte。
当然只是我的猜测。。。
以前那个精子文章我记得只能是male的。而且精原细胞小，而且是对称分裂，比oocyte
形成和分裂简单得多。 |
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s******y
发帖数: 28562 | 6 所以这个就是一个关键问题，就是XY细胞是否能够成为oocyte?
貌似以前的那个精子的文章里，（不知道是不是我记错了）XX细胞也能被诱导
成精子的？ |
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s******y
发帖数: 28562 | 7 XXY的人能产生卵子么？
难道他们不都是不可育的群体么？
oocyte |
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a*****x
发帖数: 901 | 8 这篇文章开头就有的：
“We have shown previously that embryonic stem cells (ESCs) and induced
pluripotent stem cells (iPSCs) from male (XY) mice are induced into epiblast
-like cells (EpiLCs), which are in turn induced into primordial germ cell–
like cells (PGCLCs) with proper function for spermatogenesis and offspring
production”
所以精子用的是XY
oocyte |
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s*******e
发帖数: 1389 | 9 不育男的的体细胞能去分化成精子就可以了（Y sperm），再和你喜欢的女的的卵子人
工受精一下。这个oocyte给不育女的用的。 |
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a*****x
发帖数: 901 | 10 epigenetics marker应该是被reset了，否则怎么变成oocytes呢。telomere length应
该在iPSC的时候就变了，因为激活了telomerase。
凭感觉XY还是不能诱导为卵子，感觉如果可以的话Single X的人应该也能产生卵子。不
过值得试一试。我猜他们应该也试过，还没有成功。
我个人认为在人的细胞中重复这个发现比做这个性别错乱更有实用价值。 |
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m***i
发帖数: 4637 | 12 联想到haploid是可以活下来
然后再走sperm或oocyte |
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s*******e
发帖数: 1389 | 13 理论可以？至今为止iPSC到sperm or oocyte的过程都是黑盒子，最复杂的一点就是PGC
如何与niche cell communicate。举个例子（我不是做老鼠的，业余看的），male PGC
：SRY on Y chromosome--->SOX9--->FGF9--->(gonad cell->sertoli cells）--->
male PGC
如何让XX iPS cell 与male gonad interaction？ |
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u**********d
发帖数: 573 | 14 应该说为数不多的露天矿或者浅层矿。再往后的器官、组织的协调发育就更难了。如果
真如你所说的几年后就可以用dish来长出oocytes了，那么期待有朝一日诱导产生各种
器官的bud，丢到in vitro的培养体系里就长出肝啊肾什么的。 |
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a*****x
发帖数: 901 | 15 你要先弄清楚oocyte和egg（或者ovum）的区别 |
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l**********1
发帖数: 5204 | 16 sorry
当时走神
看成
应该说为数不多的露天(铜)矿或者浅层(铁)矿 了 然后回的帖
不过 后来一琢磨 美帝No. 51 base 里的外星人标本 金属含量
尤其是iron 可能远高于人体内的iron 含量 -------just one cent
>
>>
发信人: unfieldified (未场化的蛮), 信区: Biology
标 题: Re: oocytes derived from mESC/iPSC develop into normal, fe
发信站: BBS 未名空间站 (Fri Oct 5 16:41:45 2012, 美东)
应该说为数不多的露天矿或者浅层矿。再往后的器官、组织的协调发育就更难了 |
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发帖数: 1 | 17
Download pdf published by SfN (Chapter from 'The History of Neuroscience in
Autobiography, Volume 8' edited by Larry R. Squire)
Yuh-Nung Jan's CV
Lily Jan's CV
Yuh-Nung Jan and Lily Jan
Birth
Family History and Growing Up
National Taiwan University
The Hiking Trip to Shitou in the Spring of 1967
Graduate School Application
Graduate Study at Caltech (1968锟974)
Seymour Benzer Lab (1974锟977)
Steve Kuffler锟絪 Lab at H... 阅读全帖 |
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l*****9
发帖数: 9501 | 18 http://www.businessweek.com/news/2012-02-27/ovarian-stem-cells-
Ovarian Stem Cells Make Human Eggs in Possible Aid to Fertility
By Ryan Flinn
Feb. 27 (Bloomberg) -- Stem cells taken from human ovaries were used to
produce early-stage eggs by scientists in Boston who may have created a new
method to help infertile women.
Females have a fixed number of eggs from birth that are depleted by the time
of menopause. The finding, published today in the journal Nature Medicine,
challenges the belief that... 阅读全帖 |
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n********k
发帖数: 2818 | 19 Joke of the day or what?
there is not a most shameless but more shameless!
Results: 33
1.
Viable fertile mice generated from fully pluripotent iPS cells derived from
adult somatic cells.
Zhao XY, Li W, Lv Z, Liu L, Tong M, Hai T, Hao J, Wang X, Wang L, Zeng F,
Zhou Q.
Stem Cell Rev. 2010 Sep;6(3):390-7.PMID: 20549390 [PubMed - in process]
Related citations
2.
Production of mice using iPS cells and tetraploid complementation.
Zhao XY, Lv Z, Li W, Zeng F, Zhou Q.
Nat Protoc. 2010;5(5):963-71. Epu... 阅读全帖 |
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b*****g
发帖数: 119 | 20 谢谢~ 你们都好热心
是这样的, 老鼠在出生后不久绝大多数的primary oocyte就开始生长了, 然后我只是在
25天左右的时候给激素, 2天后提取oocyte. 给激素的目的其实就是更进一步促进
follicle and oocyte growth 等到我提取的时候他们就是已经很成熟的meiotically
competent的卵子了 所以我认为跟我打针后2天就取卵关系不大. zp3 应该出生后不久
就开始表达了 倒是越到后来表达变多倒是很有可能.
那些静息的形态很明显, 这种的卵子和follicle之间还没有形成antrum 所以我是不会
用的.
然后哺乳动物的oogenesis比较奇特 不是contineous process, arrest at prophase I
. 所以在我分离oocyte的时候他们还是在2倍体. 按理说如果cre 有效果的话就已经把2
个都切了
我想法子做个oocyte' genotype或者RT把, 如果不work的话我打算一个allele的敲 做
老鼠genetics 真是费时间阿 T-T |
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l**********1
发帖数: 5204 | 21 Please refer
Swedish Umeå Universitet. [2007] one PhD Dissertation
title:
Studies of phosphatidylinositol 3 kinase (PI3K) signaling pathway in
mammalian ovarian follicle activation and development
University dissertation from Umeå : Medicinsk kemi och biofysik
AUTHOR: Singareddy Rajareddy
KEYWORDS: oocyte; follicular development; P13K pathway; Medical Biochemistry
; medicinsk biokemi;
ABSTRACT: ignored and (iii) the overactivated follicles in p27-/- ovaries
are depleted in early adul... 阅读全帖 |
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h******y
发帖数: 351 | 22 终于克隆人了。
Human Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer
Cell, 15 May 2013
doi:10.1016/j.cell.2013.05.006
Reprogramming somatic cells into pluripotent embryonic stem cells (ESCs) by
somatic cell nuclear transfer (SCNT) has been envisioned as an approach for
generating patient-matched nuclear transfer (NT)-ESCs for studies of disease
mechanisms and for developing specific therapies. Past attempts to produce
human NT-ESCs have failed secondary to early embryonic arrest of SCNT e... 阅读全帖 |
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k*****e
发帖数: 22013 | 23 【 以下文字转载自 Queer 俱乐部 】
发信人: university1 (guagua), 信区: Queer
标 题: Generation of viable male and female mice from two fathers
发信站: BBS 未名空间站 (Mon Jul 18 00:29:26 2011, 美东)
Biol Reprod. 2011 Mar;84(3):613-8. Epub 2010 Dec 8.
Generation of viable male and female mice from two fathers.
Deng JM, Satoh K, Wang H, Chang H, Zhang Z, Stewart MD, Cooney AJ, Behringer
RR.
SourceDepartment of Genetics, The University of Texas M.D. Anderson Cancer
Center, 1515 Holcombe Blvd., Houston, TX 77030, USA.
Abstract
... 阅读全帖 |
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m***f
发帖数: 1622 | 24
扯淡呢，看看高最近两年发表的文章吧
CELL STEM CELL, JBC, STEM CELL, PNAS，都是很不错的
就这样还不能PROMOTION? 在美国都可以tennure了
1. Wu T., Wang H., He J., Kang L., Jiang Y., Liu J., Zhang Y., Kou
Z., Liu L., Zhang X., Gao S. (2011) Reprogramming of trophoblast stem
cells into pluripotent stem cells by Oct4. Stem Cells 2011 Feb 8.
[Epub ahead of print].
2. Kang L., Wu T., Tao Y., Yuan Y., He J., Zhang Y., Luo T., Kou Z.,
Gao S. (2010) Viable mice produced from 3-factor induced pluripotent
stem (iPS) cells through tetrapl... 阅读全帖 |
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s******s
发帖数: 13035 | 26 想转细胞核到其他细胞里，但是不是oocyte，这个是不是比往oocyte里搞更加难做？
有什么实验室做这样的实验么？ |
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i***l
发帖数: 1656 | 27 agree this, partially, and zan the upstairs suggestion on frog oocytes
1. localization
2. in vitro function, and mutagenesis
xenopus oocyte is my #1 suggestion, HEK293 , ,,,,,
radio activity labeled substrate uptake should be easier if it's neutral
transporter
NOTE electrophysiology requires special training, but it works well ,as long
as target transports ionic stuffs or is ph dependent
3. in vivo funtion
btw it could be channel instead of transporter, who knows
btw, you'd better determine t... 阅读全帖 |
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a*******a
发帖数: 4233 | 28 The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes.
Nature, 477,606-610.
在oocyte里面做的比较多， 不过实验材料不好搞做起来很麻烦。 |
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b*****g
发帖数: 119 | 29 新人求教 我在做一个基因的oocyte specific KO 拿到正确genotype的老鼠后 去做
staining 那个蛋白居然还在... 请问会是些什么原因呢 我的mating scheme如下
Cre+ ; fl/+ male x fl/fl female 得到 Cre+ fl/fl female
现在想到的问题有一下几个 1.可能蛋白在Cre前就已经表达了
2. 同时excise 两个有loxP的allele是不是不够efficient阿? 由于我做的是oocyte 所
以尽量选择让公老鼠carry Cre, 因为是homozygous lethal 我不是不是需要一步一步
的去除基因呢
请问大家我该如何着手解决这个问题? 谢谢了! |
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D*a
发帖数: 6830 | 30 cre刚切完还有蛋白也是可能的。flox/flox我觉得不影响cre的效率的，cre好用的话一
个两个都无所谓，不好用的话就是不好用了。
你做oocyte 的 genotype了么，是什么结果？直接上staining看蛋白了？当然oocyte
特异PCR不好做，但是你自己想办法看见带吧。
如果cre f/f的母老鼠跟+/- 公老鼠交配，后代应该是一半纯和致死的，你观察到了么？
你现在的信息说这个cre好用不好用都太早。 |
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f*******n
发帖数: 141 | 31 这个cre在growing follicle表达，看别人文章，效率还是不错的。每次实验要分离
oocyte，不能直接用ovary做，是挺麻烦的。可能还是抗体的特异性不好吧，如果是你
这个蛋白能在granulosa cells和oocyte之间转运，那你要发达了。 |
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E**********t
发帖数: 56 | 32 Xuebiao Yao, Dasheng Li and Gang Pei recently wrote an essay to Nature
Review in MCB Which is entitled “In focus: molecular and cell biology
research in China”. Here is the link: http://www.nature.com/nrm/journal/v14/n9/full/nrm3638.html In this article, they discussed the past, present and future of molecular and cell biology research in China. One part which interested me is the key MCB discoveries in China. Although most of the work are nice and likely solid work, few of them are breakthrough... 阅读全帖 |
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L*********y
发帖数: 322 | 33
of
What is the Kd of this compound? Does the inhibition depend on the presence
of the cargo carried by the transporter? You don't have to do this
experiments in lysis buffer. The transporter protein, ideally with a tag can
be prepared from oocyte, then you throw the labelled compound in the oocyte
system. After pulling-down the transporter, the binding can be analyzed
through assaying the amount of compound incorporated into this transporter.
findings?
I agree it is not a direct biochemical exp... 阅读全帖 |
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z*t
发帖数: 863 | 34 nature有一篇文章说其实是oocyte发生了parthenogenesis，他以为拿到了而已。
这些oocyte大都是他lab的女性员工捐的，她们捐完卵要马上回去干活... |
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m****l
发帖数: 16 | 35 今天刚看到Molecular Cell 的 Erratum。 有些看不懂了。
CRL4VprBP E3 Ligase Promotes Monoubiquitylation and Chromatin Binding of TET
Dioxygenases
Molecular Cell, Volume 59, Issue 6, p1043, 17 September 2015
During the final preparation of this manuscript, the authors accidentally
omitted the reference and discussion of the following paper: Yu et al. (2013
). CRL4 Complex Regulates Mammalian Oocyte Survival and Reprogramming by
Activation of TET Proteins. Science 342, 1518–1521. The reference should
have appeared... 阅读全帖 |
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m****l
发帖数: 16 | 36 今天刚看到Molecular Cell 的 Erratum。 有些看不懂了。
CRL4VprBP E3 Ligase Promotes Monoubiquitylation and Chromatin Binding of TET
Dioxygenases
Molecular Cell, Volume 59, Issue 6, p1043, 17 September 2015
During the final preparation of this manuscript, the authors accidentally
omitted the reference and discussion of the following paper: Yu et al. (2013
). CRL4 Complex Regulates Mammalian Oocyte Survival and Reprogramming by
Activation of TET Proteins. Science 342, 1518–1521. The reference should
have appeared... 阅读全帖 |
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L*****r
发帖数: 722 | 37 Future AMG and current CMG 都力顶的，想必是好帖。:-)
坐飞机一下睡了7个小时, DVT, 大面积PE，这样的病例不会上NEJM阿，古怪是在
retrieve oocyte to have posthumurous offspring上？取oocyte时候发现肿瘤了？卵
巢癌（ovarian cancer）、绒癌(choriocarcinoma) 啥的，一是可能直接造成
pulmonary tumor embolism，二是可能造成hypercoagulability，easier to form DVT
and PE from DVT.
一时想不起别的可能了。 |
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s*********e
发帖数: 36 | 38 这个是houston诊所的数据
Fresh Embryos From Non-Donor Oocytes <35岁
Number of cycles 132
Percentage of cycles resulting in pregnancies 54.5
Percentage of cycles resulting in live births 50.8
这个是我现在所在的诊所的数据
Fresh Embryos From Non-Donor Oocytes <35岁
Number of cycles 82
Percentage of cycles resulting in pregnancies 30.5
Percentage of cycles resulting in live births 29.3
我所在的诊所做的例数不算很少，只是成功率低了太多 |
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C****g
发帖数: 2220 | 39 真是“大道之行简也”。。。
Summary
Differentiated cells can be reprogrammed to an embryonic-like state by
transfer of nuclear contents into oocytes or by fusion with embryonic stem (
ES) cells. Little is known about factors that induce this reprogramming.
Here, we demonstrate induction of pluripotent stem cells from mouse
embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-
Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was
dispensable. These cells, which we desi... 阅读全帖 |
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g***j
发帖数: 40861 | 40 【 以下文字转载自 Biology 讨论区 】
发信人: SWJSJ (BC), 信区: Biology
标 题: 单开一贴，浙大范衡宇涉嫌更加严重的剽窃和学术不端！
发信站: BBS 未名空间站 (Thu Sep 17 23:38:04 2015, 美东)
靠，实在是看着不爽，凭啥清北撕逼就大家一致讨伐，浙大范衡宇更加恶劣的剽窃别人
成果却没人关注？下面转帖，是从张生家和谢灿那个帖子里看到的，其他几位ID不愿意
单开一贴，我实在看不下去，特开一贴。这个范衡宇据说还拿到了杰青，国内科研真是
尼玛无底线了啊。
如果有知道内情的，在此帖讨论吧，不然都被清北的刷屏了。当然，也许有其他隐情冤
枉了范衡宇，也可以内部人出来解释解释。如果确定是剽窃，这尼玛妥妥的应该上新语
丝头条啊。
以下转帖：
----------------------------------------------------------------------------
------------------------
发信人: forjoy (三分华盖), 信区: Biology
标 题: Re: 能拿炸药奖吗？
... 阅读全帖 |
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w*********o
发帖数: 3030 | 41 Look from the bright side, now the government can provide make-to-order
Chinese babies, maybe there would be less child abduction?
I wonder if the rumor about Xi's illegitimate son is true or not. If not,
the technology is advanced enough to find the precious live sperm he still
might have. Can pick the best oocyte he want, for sure not from the paid
trolls frequent this website, too low average IQ. |
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l**********r
发帖数: 240 | 42 This is hard to say:)
Certainly it is easier for people to try to make a baby if the menstrual cycle
is precise. Because then you can decide when to try knowing your ovulation
time, since the oocyte does not last forever before it can't be conceived any
more.
You can estimate your ovulation time by three methods:
1. Basal temperature
2. Cervical mucus
3. Ovulation kit sold in drugstore |
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f******I
发帖数: 769 | 43
honestly i have no idea how you females have things working in there, but if
the pipe is open, all you have is just one egg(or oocyte) to pass through
the fallopian tube after the surge of luteal hormone, right? |
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f******I
发帖数: 769 | 44
honestly i have no idea how you females have things working in there, but if
the pipe is open, all you have is just one egg(or oocyte) to pass through
the fallopian tube after the surge of luteal hormone, right? |
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s********e
发帖数: 1596 | 45 The major risk for advanced maternal age is chromosomal abnormalities, i.e.
trisomy (3 copies of a particular chromosome) or monosomy (a single copy of
chromosome) caused by errors of meiosis of the oocytes. Most monosomy cases
die early during embryogenesis or early fetal stage. For trisomies, the ones
that can survive are trisomy 13, trisomy 18 and trisomy 21, with the last
one being Down Syndrome. In most cases, trisomy 18 and 13 die before they
reach 1 year of age. Trisomy 21 can live to adu... 阅读全帖 |
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z*****g
发帖数: 1 | 46 The Reproductive Medicine Center of the Third Affiliated Hospital of
Guangzhou Medical University has an open position in an IVF clinic lab.
The ideal candidate should have a Ph.D. degree in Biology, Cell Biology,
Developmental Biology, Molecular Biology, Biochemistry, Animal Sciences,
Genetics, Medicine or other related fields. He or she should be able to work
on in vitro oocyte and embryo culture, in vitro fertilization, and other
clinic service in an IVF lab, in addition to research on human ... 阅读全帖 |
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i*****y
发帖数: 44 | 47 祝福楼主，不知道楼主什么研究方向，有多出来的paper review机会转让给本人么？蛋
白质结晶学，分子生物，微生物，细菌抗药性，钾离子通道小分子药物，电生理等。。。
本人英国博士，structural biology方向，主要技术是膜蛋白纯化，结晶和晶体解析，
研究方向是通过解析膜蛋白结构阐述细菌抗药机理。
现在美国博士后，一直做细菌抗药分子机理相关方向，最近两年研究钾离子通道跟疾病
的关系，主要技术是电生理 （two electrode voltage clamp in oocytes), yeast
assay, structural-function study..。
恳求有机会转让给我。email: l*******[email protected] |
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