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f**d
发帖数: 768 | 2 这是一本计算神经科学的优秀著作，全文拷贝这里（图和公式缺），有兴趣的同学可以
阅读
如需要，我可以分享PDF文件（－－仅供个人学习，无商业用途）
From Computer to Brain
William W. Lytton
From Computer to Brain
Foundations of Computational Neuroscience
Springer
William W. Lytton, M.D.
Associate Professor, State University of New York, Downstato, Brooklyn, NY
Visiting Associate Professor, University of Wisconsin, Madison
Visiting Associate Professor, Polytechnic University, Brooklyn, NY
Staff Neurologist., Kings County Hospital, Brooklyn, NY
In From Computer to Brain: ... 阅读全帖 |
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p*****c
发帖数: 20445 | 3 Christopher D. Lima, Ph.D.
Memorial Sloan-Kettering Cancer Center
Christopher Lima doesn’t pick easy problems. A structural biologist, he is
investigating how cells attach small proteins—like ubiquitin and the
related molecule SUMO—to other proteins to modify their function or fate.
Alongside those studies, he is exploring the molecular mechanisms that
underlie RNA processing and degradation—modifications to an RNA copy of a
gene that influence its stability and ability to be used as a template ... 阅读全帖 |
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l*****a
发帖数: 38403 | 4 Point out that science is a wonderful thing, but discuss "natural" processes
like the ones called entropy (definition: disorder in a system, which
resolves to equilibrium--like dust settles) and chaos (definition: inherent
unpredictability) are not thought of as building anything:
The second law of thermodynamics states that the entropy (or disorder) of
the entire universe (a system comprising of ALL matter) must increase with
time. If the universe had always existed, then entropy of the entire
... 阅读全帖 |
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l*****a
发帖数: 38403 | 5 Explain that there is no simple cell. It has many dependencies, not just
thousands but actually millions and billions of genetic processes including
the very basic system of genes that pass instructions for self-manufacture
of life by that specific form of life -- and perhaps, epigenetic tags that
act in an approximately stepwise process by which genetic information is
thought to be translated into the substance and behavior of that specific
organism.[2]
There is ingestion using an organized and... 阅读全帖 |
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l**********1
发帖数: 5204 | 6 Cryo-Electron Tomography
Cryo-ET is a very accurate way to determine the three-dimensional structure
of a specimen because the rapid freezing of the object and cryogenic
temperatures gives a good preservation of the structure and good time
resolution of certain processes. For example, the rapid freezing of cells
and tissues at a certain point in cellular processes can give a good
understanding of the structure and activity of those cells and tissues at a
certain point in time during that particu... 阅读全帖 |
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l**********1
发帖数: 5204 | 7 1)
from it is more exciting:
please focus on
Allometry and stoichiometry of unicellular, colonial and multicellular phytoplankton
by Population Genetics even Stochastic model methods
please go to below paper:
PubMed link:
//www.ncbi.nlm.nih.gov/pubmed/19121029
full text link:
//www.mmab.ca/lib/exe/fetch.php?media=pubs:beardall-2008-new-phytologist-
tansley.pdf
more please go to
allometric relationship between organism metabolism and body mass
recent paper:
Heritability is not Evolvability
Abstra... 阅读全帖 |
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w***r
发帖数: 709 | 8 扯淡一样的故事，关键是不是圈中人，我是捂裆派的。
Science. 2015 Jan 2;347(6217):83-6. doi: 10.1126/science.1258857.
Aging. Lysosomal signaling molecules regulate longevity in Caenorhabditis
elegans.
Folick A1, Oakley HD2, Yu Y2, Armstrong EH3, Kumari M4, Sanor L5, Moore DD6,
Ortlund EA3, Zechner R4, Wang MC7.
Author information
Abstract
Lysosomes are crucial cellular organelles for human health that function in
digestion and recycling of extracellular and intracellular macromolecules.
We describe a signaling role for ly... 阅读全帖 |
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j****g
发帖数: 406 | 9 谢谢老大的建议，下面是introduction的第一段。版面有限估计贴不了更多的了，如果
老大，或者是其他哪一位有兴趣的话我可以发到邮箱里。谢谢。
The balance between protein synthesis and degradation is important for
normal function and survival of cells. While the ubiquitin-proteasome
pathway is responsible for specific clearance of short-lived proteins, long-
lived proteins are delivered to lysosomes by autophagy, which is the major
inducible pathway for general turnover of bulk cytoplasmic components
including entire organelles such as mitochondria (Klionsky and ... 阅读全帖 |
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w********2
发帖数: 632 | 10 HGT involving eukaryotes
The best recognized examples of HGT in eukaryotes in- clude the wholesale
absorption of bacterial genomes that once existed as endosymbionts or
parasites and now ex- ist as remnant genomes, such as mitochondria and plas-
tids (Gray, 1993). This process of ‘you are what you eat’ (Doolittle,
1998) has continued throughout evolu- tion and includes gene acquisitions
from both prokary- otic (Hotopp et al., 2007) and eukaryotic endosymbionts (
Li et al., 2006; Nosenko and Bhat... 阅读全帖 |
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B*******r
发帖数: 65 | 11 Applications are invited for a junior lab technician position in a research
lab The Laboratory Technician will support senior lab members in structural
studies of macromolecular assemblies and sub-cellular organelles in their
functional context using modern techniques of 3D electron microscopy. Work
assignment includes specimen preparation, data collection using Scanning
Electron Microscope and/or Transmission Electron Microscope, and routine
computational image processing. The initial appointm... 阅读全帖 |
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m*******n
发帖数: 5103 | 13 此法案又称 GMO Labeling，于去年提出，在搜集足够签名后由州务卿咨请州议会表决
，但州议会并未进行讨论与表决。根据法律规定，未讨论的法案在第二年的普选时交由
选民公投，也就是今年的 11/5。如果通过，将在 7/1/2015 实施。
法案要求所有经过基因改造的食品，或者含有基因改造成分的食品，都必须明确标示。
正方认为这是民众知的权利，反方认为这会提高食品价格，尤其对本州的中小企业不利。
敬请有投票权的同学们关注此案，正反双方的意见请自行参考以下网站：
正方（主张必须标识）网站 http://yeson522.com/
反方（反对标识）网站 http://factsabout522.com/
阴谋论者可能会觉得反方一定是邪恶势力，但反方除了大公司以外，也包括本地
的中小企业。反对的原因除了提高成本，伤害本地经济以外，也有人说此法案本身不够
健全，如果实施，反而抓小放大，误导消费者。
重点是：天下事情没那么简单 --- 邪恶势力 vs. 屁民，正反双方的意见都有他们的道
理。建议同学们学习学习再决定。
此法案只是说基因改造的食品必须标识。至于基因改造的食品对人究竟有害无害，不是... 阅读全帖 |
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t*******h
发帖数: 2744 | 14 The United States of Basic Bitches: A Map and Field Guide
579,23057
morninggloria
Erin Gloria Ryan
ProfileFollow
Erin Gloria Ryan
Filed to: AMERICA THE BASIC
BASIC BITCHES
6/30/14 11:20am
Share to Kinja
Share to Facebook
Share to Pinterest
Share to Twitter
Go to permalink
The United States of Basic Bitches: A Map and Field Guide
America can consider itself #blessed that it has a word like "bro"— three
letters that convey to most people a specific image about a specific
regionally varietal typ... 阅读全帖 |
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l*********1
发帖数: 2971 | 15 Egg Quality
Egg quality is often blamed as the cause for IVF failure. Improving egg
quality gives you a better outcome from subsequent IVF cycles and also
contributes to having a healthy pregnancy and baby.
One of the reasons that the focus is more on egg quality than sperm quality
is that the egg contributes all the mitochondria for the embryo.
Mitochondria are tiny organelles in every cell of your body where energy is
produced. All the mitochondria in the embryo come from the egg – none come
f... 阅读全帖 |
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J*****3
发帖数: 4298 | 16 笑死我了，还来玩黑白颠倒的吗？
我给你全文吧，这么撒谎的话，是耶稣教的吗？撒谎神不喜悦
这篇文章是基督教手册用来歪曲人类进化不成立的，是JAMES给我了文章的一个LINK，但是文章看不全，我根据作者名字找到了全文，也读了，发现，文章根本没有说进化不成立，他们断章取义，黑白颠倒地撒谎。我BS这种恶心的行为。
“5、大概在97年，一个著名的非基督徒考古学家，在非洲发现的所谓人类化石，把一
直以来公认的人类进化顺序，猿人——直立人——能人——智人，给彻底推翻了。目前
人类进化链条重新归零，变为空白。”
原文
http://news.nationalgeographic.com/news/2010/05/100506-science-
翻译：
“Even if the new result doesn't quite settle the debate about whether
Neandertals mixed with modern humans, it does underscore how different they
were from our own lineage. And t... 阅读全帖 |
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K****N
发帖数: 10783 | 18 I know it sounds corny and silly but today in my bio lab we looked at a
plethora of different cells. But the first one we looked at, of a leaf of
all things, absolutely blew my mind. I can't describe how I felt in words,
it was a feeling of looking at the building blocks of life. And when I saw
the organelles shifting around and actually saw that it was alive...not to
sound kinda girlie or anything but I felt like crying. It was so beautiful.
EDIT: Some people have been hating on the fact that I |
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r****o
发帖数: 105 | 19 It is OK! Actually that's the whole basis for FACS analysis.
The point of fixation is not to denature the protein. It just
crosslinks the proteins and make the organelle structures stable in
a cell.
The antibodies used in IF are usually raised against antigen
in its native structure. |
|
c***o
发帖数: 35 | 20 The perfect storm of tiny RNAs:
"We can expect to find small RNA action in synaptic signaling, in the regula
tion of cell division, in the regulation of organelle trafficking within cel
ls, and so on."
Personally I don't think microRNA as trans-synaptic signaling molecules woul
d make much sense, although it certainly has some functions within the pre-
or post-synaptic compartments. |
|
f**u
发帖数: 346 | 21 从某种意义上说，我觉得wolbachia感染昆虫的germline，
并通过germline一代一代往下传，这个可以算是lateral evolution的一个例子。
你甚至可以把wolbachia看成是一个organelle。
件， |
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n***w
发帖数: 2405 | 22 would you please be more specific?
If it's inactive in the cytosol, and it is redistributed to membrane after
phosphorylation. It may be due to changes to cytoskeleton and motor proteins.
If it is normally a nuclear protein or cytoplasm protein, somehow it is
translocated into the subcellular compartment like mitochondria or other
organelles, it may interact with some proteins that have those translocation
signals. |
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p*****m
发帖数: 7030 | 23 许田很赞 他的mosiac系统在果蝇发育生物学里的作用是革命性的 但是要说一般性的地
位 他的工作还是不如小东吧。毕竟他用的flp-FRT系统（尤其结合hs-flp）基本只在果
蝇里面用得好 而且毕竟这套工具性的系统过去20年也很难说产生过breakthrough的发
现（当然这个不能怪许田自己） 尽管是个人可能都免不了要用一用。
晓东的工作不光是发现cyto c的问题 那是一整条signaling pathway的问题。而且把线
粒体这个organelle整个扯进apoptosis也是很breakthrough的 |
|
p*****m
发帖数: 7030 | 24 许田很赞 他的mosiac系统在果蝇发育生物学里的作用是革命性的 但是要说一般性的地
位 他的工作还是不如小东吧。毕竟他用的flp-FRT系统（尤其结合hs-flp）基本只在果
蝇里面用得好 而且毕竟这套工具性的系统过去20年也很难说产生过breakthrough的发
现（当然这个不能怪许田自己） 尽管是个人可能都免不了要用一用。
晓东的工作不光是发现cyto c的问题 那是一整条signaling pathway的问题。而且把线
粒体这个organelle整个扯进apoptosis也是很breakthrough的 |
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S*********s
发帖数: 304 | 25 很多时候不是shRNA序列的问题
很多organelle localized的protein的turnover时间就是比较长。
即使mRNA knockdown了，蛋白降解还需要很长的时间。
另外跟cell line也有关系。
Barcode。 |
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b******s
发帖数: 1089 | 26 我不是专家。我也是一边做一边学。而且我不是做动物的，对于whole brain的大小没
概念。但是印象中PFA penetration的速率好像是1小时1mm左右，你自己可以估计一下
。我想多半还是太大，应该需要slice，并且需要4度overnight。小sample 2hr足够fix
的不必overnight，overfix也不好。但是好像很多人都是丢进fixative过夜。所以应该
区别也不大。但是最好是4度，否则cellular extraction和其他artifacts会比较严重
。sample stress在化学固定过程中或多或少，不可避免。除非高压冷冻迅速fix
sample。在电镜下会看的比较明显。plasma membrane会很wavy，一些organelle形态异
常，甚至aggregate。
别。 |
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l**********0
发帖数: 135 | 27 转信息
姓 名 傅瑾
性 别 女
出生年月
专业技术
职 务 教授
所获学位 博士
授予单位 美国哥伦比亚大学
研究方向
学科专长
联系电话 0592-2187210
E - MAIL f***[email protected]
通信地址 厦门大学路168号文正楼502
邮 编 361005
个人中文简介
傅瑾，1967年生人，闽江学者。1999年10月，在美国哥伦比亚大学获博士学位。
1999—2000年，纽
约哥伦比亚大学神经与遗传发展学院，博士后；2000—2001年，美国加利福尼亚大学药
学系做博士后；
2001—2003年，美国加利福尼亚大学药学系副研究员；2002—2008，在美国 Kadmus 制
药公司任顾问；
2003-2008，美国加利福尼亚大学药学系任研究员；2005年10月29日，厦门大学医学院
任特聘讲座教
授。2009年9月1日，厦门大学医学院任特聘教授。在国际重要杂志上发表研究论文30篇
(SCI收录），包括Nature、Nature系列杂志等。主持科研项目经费（折合成人民币）
1000万... 阅读全帖 |
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h**********r
发帖数: 671 | 28 Silver组里搞得还挺多的：
Modularity of a carbon-fixing protein organelle
www.pnas.org/cgi/doi/10.1073/pnas.1108557109
另外我记得还有一篇在核酸研究上的paper,利用酵母的homologous做的组装，不知道和
日本人不是差不多的 |
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d*****r
发帖数: 2583 | 29 The ultimate goal of cell biology tool, is an imaging system like a
helicopter or a submarine, that can take you inside the live cells, and see
all the genes, proteins, and organelles interacting with each other. |
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l**********1
发帖数: 5204 | 30 Re LX request: 能不能给列出几个这样做的组?
Here you go
http://www.csh-asia.org/design11.html
Keynote Speakers:
Jay Keasling, University of California, Berkeley, USA
Invited Speakers:
Adam Arkin, University of California, Berkeley, USA
Yaakov (Kobi) Benenson, ETH Zurich, SWITZERLAND
Austin Burt, Imperial College London, UK
Jeff Boeke, Johns Hopkins University, USA
Michelle Chang, University of California, Berkeley, USA
Virginia Cornish, Columbia University, USA
John Dueber, University of California, Berkeley... 阅读全帖 |
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l**********1
发帖数: 5204 | 31 Re LX request: 能不能给列出几个这样做的组?
Here you go
http://www.csh-asia.org/design11.html
Keynote Speakers:
Jay Keasling, University of California, Berkeley, USA
Invited Speakers:
Adam Arkin, University of California, Berkeley, USA
Yaakov (Kobi) Benenson, ETH Zurich, SWITZERLAND
Austin Burt, Imperial College London, UK
Jeff Boeke, Johns Hopkins University, USA
Michelle Chang, University of California, Berkeley, USA
Virginia Cornish, Columbia University, USA
John Dueber, University of California, Berkeley... 阅读全帖 |
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b******y
发帖数: 627 | 32 The round-shaped entities seem to be some non-membranous organelles. The
protein might be multi-valent scafolding protein or a granule (such as
stress granule, processing body) localizing proteins. |
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h*******o
发帖数: 4884 | 33 yep, looks like lysosome to me too
LZ can get some cellular organelle marker and try double staining. |
|
i*****g
发帖数: 11893 | 34 subcellular organelle isolation never pure enough |
|
F*Q
发帖数: 3259 | 35 教科书都这样，之乎者也、莫须有的广为散播，难怪那么多的烂东西都能整到CNS、被
捧为经典和教条。生命科学真的任重道远啊！ |
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r**b
发帖数: 81 | 36 Sure, no cure for Schizophrenia either. |
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S**********e
发帖数: 1789 | 37 洗洗睡吧，需要你操心的事很多，这些大事情不用吾辈操心了 |
|
F*Q
发帖数: 3259 | 38 If it is bothering you, let me know. I may be able to help you |
|
F*Q
发帖数: 3259 | 39 因为生物背景弱，想学点知识，特意找来一本教科书。碰到似懂非懂的东西，来这里请
教（生物界的大事显然轮不上一个民科操心），居然没有人屑于赐教？ |
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a**********2
发帖数: 3726 | 40 Lol, trying to be sarcastic? |
|
x********a
发帖数: 6 | 41 Analysis of membrane-bound organelles.
Fares H, van der Bliek AM.
Methods Cell Biol. 2012;107:239-63.
非常感谢！ |
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A******y
发帖数: 2041 | 42 It really doesn't matter if it is chemical modification or biological
modification. Some scientist in the field believe a lot of acetylation
modification is through direct chemical transfer of Acetyl-CoA without the
help of HAT espeically in organelle enviroment favoring nucleophilic
addition.
As far as crotonyl-modification. My friesd published in a good journal
stating HDAC3 is the de-crotonylase...my lab results are exactly opposite.
HDAC1 and HDAC2 have more activities against cortonyl-ly... 阅读全帖 |
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h**********r
发帖数: 671 | 43 A very good paper!
How about organelles such mitochondria and peroxisome? Do you have any
recommendation?
Thanks! |
|
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P*******D
发帖数: 523 | 45 恩，我们正在做organelle specific的probe。目前作为global GSH的monitor没有问题
，也很方便。live animal，我估计zebrafish没问题, :)
如果是mice，就有荧光penetration的问题，我们也在想一些办法解决这个，但是确实
想做到animal上。 |
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P*******D
发帖数: 523 | 46 Thanks, ArtyArty! This probe does not have organelle specificity, as we
discussed in the paper. so we cannot distinguish between the cyto and mito
pools of GSH. this will certainly be our next step.
In terms of toxicity, we have not measured IC50 yet. during the 1-2 h
experiment, we did not observe any apparent toxicity. However, this does not
mean it does not have long term toxicity.
The highest probe concentration we used was 1 uM. For HeLa cells, we can use
the concentration as low as 20 nM. ... 阅读全帖 |
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A******y
发帖数: 2041 | 47 I think confocal microscopy with organelle specific dye (i.e. mito-tracker)
together with your probe will be able to tell if mitochondrial GHS is
depleted in mitochondria.
not
use
. |
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a*****i
发帖数: 2108 | 48 Try to add signal peptides leading your protein to other organelles. |
|
r*******e
发帖数: 123 | 49 请发到我的邮箱: [email protected]/* */
万分感谢
Actin Cytoskeleton-Mediated Constriction of Membrane Organelles via
Endoplasmic Reticulum Scaffolding
Carol Lynn Curchoe and Uri Manor*
Waitt Advanced Biophotonics Center, The Salk Institute for Biological
Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, United
States
ACS Biomater. Sci. Eng., Article ASAP
DOI: 10.1021/acsbiomaterials.6b00802
Publication Date (Web): February 9, 2017 |
|
H*****9
发帖数: 11 | 50 I now discuss some of the Chinese Classical literature and prep the patient
mentally:
The Huang Di Nei Jing states that the organs produce Qi and that the Qi of
the
different organs have differing functions. However, in healthy people the Qi
of
each organ feeds and balances the receiving organ. This Qi must then travel
from
the Organs to nourish the ends of the fingers, nose, toes, and brain. So, we
have this simplified idea of Qi from the Huang Di Nei Jing. But the magic
questions are, “What is... 阅读全帖 |
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