r****o
发帖数: 105 | 1 本文献给YL
（十六）O Infundibulum, Where Art Thou?
上一篇介绍了胚胎发育的原肠化期（6.5dpc~7.5dpc）。在此之前，胚胎并没有成
形，甚至看不出来头尾。7.5dpc之后，胚胎进入器官形成期（organogenesis stage）
。这一时期中，最早开始发育的是神经系统，前方外胚层衍生出神经板（neural plate
）结构，神经板再演化为神经管（neural tube）最后形成中枢神经系统，包括脑和脊
椎。神经系统的发育开始之后，整个胚胎的躯体规划已经成型，可以看出头尾。与此同
时心脏和循环系统也开始发育，随之而来的是身体其他内脏器官的发育。
终于介绍完六月七号第一天课程的实验和理论课程。现在我来介绍第二天的实验课
程。第六篇和第八篇里面我分别介绍了子宫冲洗和胚胎子宫转移两种技术。子宫冲洗的
目的是分离3.5dpc之后，处于囊胚期的着床前胚胎。但是如果要分离3.5dpc之前的胚胎
，比如单纯的受精卵，或者桑葚胚，又该怎么办呢？3.5dpc之前的胚胎还在输卵管（
oviduct），所以必须用一种叫输卵管冲洗（oviduct |
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r****o
发帖数: 105 | 2 本文献给YL
（十七）不可能完成的任务
输卵管冲洗（oviduct flushing）是第二天实验课中的第一个实验。用这种办
法分离了0.5dpc到3.5dpc胚胎之后，下一个问题就是怎么把这些胚胎放回到小鼠体内，
使它们正常发育下去。事实上有两种办法，一种是体外培养，让胚胎自己长到囊胚的阶
段，然后放回到小鼠的子宫里，也就是胚胎子宫转移的方法（Uterine Transfer）。这
种方法比较简单，但是胚胎在体外培养的条件下最后不一定能正常发育，所以最好的办
法是把这些胚胎转移到输卵管里面。这种方法叫做胚胎输卵管转移（Oviduct Transfer
）。输卵管转移是胚胎发育学最难做的一个手术，非常需要技巧。但是这个技巧又很重
要，比如要做转基因小鼠，把注射了外源DNA受精卵放回到假孕的小鼠体内发育，就需
要用这个技术。第二天以及接下来的几天中，我反复试了多次，都没有做出来。沮丧之
余，我向Anne McLauren老奶奶讨教，结果她人家说，实在是没有什么窍门，反复练习
很多次自然就会了。 |
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l*h
发帖数: 4124 | 3 people should understand tb of the lung is only a small part of the big tb
picture. tb infection of other parts also kills, in some cases with a higher
mortality than tb of the lung.
so many tuberculous infections are common in China: lymph node, meninges,
plura, peritoneum, bone, oviducts etc. and people just choose to ignore them
when talking about numbers. |
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q*********5
发帖数: 148 | 4 非常谢谢你这么多文字的分享。
也恭喜你得到两个可爱的宝贝！
我有个问题想请教你：你是通过什么方式检查自己的输卵管是否通的？
我08年1月因为宫外孕，切掉了右边的输卵管，现在只剩下左边的了。幸好，上帝怜悯
，在08年6月，我又怀孕，现在儿子5岁了。我一直很想再要个孩子，可是，总感觉左边
有时会痛。今年4月，去查了下，用超音波的，医生说我左侧卵巢（oviduct）有囊肿出
血，但Fallopian tube情况看不出来。就是是否通，她看不出来。
所以，请问你怎么查才知道自己的是否通？
谢谢 |
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k******e
发帖数: 8870 | 5 this kind of things happen. one of my co-worker told me that her doctor
remove both of her oviducts without asking her permission. She knew it only
after they had been removed... So, be careful whenever you need to have a
surgery, make it clear what you want to do have and if whatever case happen,
you would like them to have your opinion involved. |
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a*******d
发帖数: 3690 | 6 The sperm is stored by the female for at least a week, in some species over
a hundred days. Then as each ovum from the ovary moves into the oviduct, it
gets fertilized with the stored sperm, producing a clutch of many eggs, all
with the sperm from that one cloacal kiss. |
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H********g
发帖数: 43926 | 7 没有发射出去的精子在睾丸里被重新吸收掉。所以输精管结扎的人不需要拖着巨蛋在地
上蠕动。
Although sperm are constantly being made within the testicles, should the
man not ejaculate for some time, his testicles won't explode. The
spermatozoa are simply re-absorbed into the body. This is important for
vasectomy patients because they simply have their 'tubes cut' and sperm is
still produced. Should excess sperm not be absorbed, it would not be long
before the patient's testicles were dragging along the ground.
不要愚昧地以为你是游得最远的一个。其实有人游得更远，一直游到了输... 阅读全帖 |
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t**********9
发帖数: 11 | 8 求paper
Hi everyone,
Can anyone help me on the following paper (a pdf copy)?
Oróstica ML, Lopez J, Rojas I, Rocco J, Díaz P, Reuquén P, Cardenas H,
Parada-Bustamante A, Orihuela PA. Estradiol increases cAMP in the oviductal
secretory cells through a nongenomic mechanism. Reproduction. 2014 Sep;148(3
):285-94.
My e-mail address: h*****[email protected]
I appreciate your help very much! |
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