W*****o
发帖数: 1780 | 1 去查了一下，张旭总共发表了90多篇英文文章（不算中文）。回国之后作为联系作者也
有快20篇了。其中有cell, neuron, Journal of neuroscience, PNAS 好多篇。而且他
1995年就回国了，那个时候国内的环境有多差不用说了吧。这也快20年了。比饶益2007
年才全职回去多了12年。比起培养的中国学生也多多了，算国内贡献肯定不会小。如果
他一直留在国外，成就未必就比饶差。谁把饶益的文章列一列。国内多少篇，总共多少
篇，培养了多少中国学生？
1. Li KC, Wang F, Zhong YQ, Lu YJ, Wang Q, Zhang FX, Xiao HS, Bao L, Zhang X
. (2011) Reduction of follistatin-like 1 in primary afferent neurons
contributes to neuropathic pain hypersensitivity. Cell Res, 21: 697-699.
2. Li KC, Zhang FX, Li CL, Wang F, Yu MY, Z... 阅读全帖 |
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O**********0
发帖数: 61 | 2 ☆─────────────────────────────────────☆
BHistory (往者的影子) 于 (Fri Sep 9 00:20:11 2011, 美东) 提到:
俺曾认为生命是进化起源的，后来越找越发现进化论像是信仰而非科学结论。可是每有
飞机网友觉着俺如此看待进化论是反科学了。
所以俺本着科学的精神，问个问题，求各位飞机进化论牛人给个科学的答案。
大家知道达尔文进化论是以遗传机制为基础的，第一个有遗传机制的生命怎么来的，当
时根本没有提及。
后人发现这一问题后，就又提出了化学进化论，就是无机分子在某种机制下，自动组合
出了第一个有遗传的生命。此后达尔文进化论似乎可以take care（其实未必，今后再
讨论）。
今天我们先看比第一个有遗传生命的出现简单万亿倍的问题，第一个蛋白质如何化学进化出
现？
先亮亮俺一个愚昧基督徒的愚见：
已知最简单的具有遗传机制的单细胞生命的复杂度已经远远超出人们最初的想象，大概
有400种蛋白质几十万个组成。其中较小的蛋白质大概100个氨基酸，如果在随机热运动
中组成，因为自然界对称的氨基酸不少于40种（20多种氨基酸及... 阅读全帖 |
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a**********i
发帖数: 48 | 3 CPC Scientific is a world-leading CMO (Contract Manufacturing Organization)
and CRO (Contract Research Organization) of proprietary and generic GMP, non
-GMP peptides and medical devices, serving customers of the pharmaceutical,
biotech, diagnostic, cosmetic, and other industries and academia.
We have a cGMP peptide facility in China which is the only non-US and non-
European facility approved by the US FDA, certified by ISO9001:2000 and
ISO13485. Additionally, we have the largest research pepti... 阅读全帖 |
|
d****y
发帖数: 2180 | 4 ☆─────────────────────────────────────☆
zhu84518 (lot) 于 (Wed Aug 8 22:41:34 2012, 美东) 提到:
A previously healthy 32-year-old woman who works as a nurse comes to the
emergency department because of a 3-week history of episodes of dizziness,
nausea, and profuse sweating that resolve with eating. She does not smoke.
She drinks four glasses of wine weekly. Vital signs are within normal limits
. Physical examination shows no abnormalities. While in the emergency
department, she becomes dizzy and... 阅读全帖 |
|
a**********i
发帖数: 48 | 5 CPC Scientific is a world-leading CMO (Contract Manufacturing Organization)
and CRO (Contract Research Organization) of proprietary and generic GMP, non
-GMP peptides and medical devices, serving customers of the pharmaceutical,
biotech, diagnostic, cosmetic, and other industries and academia.
We have a cGMP peptide facility in China which is the only non-US and non-
European facility approved by the US FDA, certified by ISO9001:2000 and
ISO13485. Additionally, we have the largest research pepti... 阅读全帖 |
|
s****2
发帖数: 4569 | 6 Director of peptide synthesis
Position Summary
We are seeking for a highly motivated scientist as a director of peptide
synthesis unit, leading a peptide synthetic group to establish peptide
synthesis facility and develop high quality peptide synthesis service at
Wuxi Apptec. The successful candidate will be expected to have extensive
experience with peptide synthesize facilities, purification technology and a
variety of peptide synthesis products.
Required Qualifications
PhD in Chem... 阅读全帖 |
|
H********g
发帖数: 43926 | 7 他们那个化学合成主要有哲学意义，或者也可能显示了一些有机合成的高超技巧。并没
有什么实际用途。
实际上我怀疑他们的路线产率应该很低，就算没有被新的合成技术代替也不可能有商业
价值。
现代肽合成是固相合成，当年发明这个的人估计已经发财了。
现在像胰岛素这样的大一点的蛋白都是用细菌或者酵母或者细胞培养来做，因为这样成
本低些。
固相合成肽：
Solid-phase peptide synthesis (SPPS), pioneered by Robert Bruce Merrifield,[
1] caused a paradigm shift within the peptide synthesis community, and it is
now the standard method for synthesizing peptides and proteins in the lab.
SPPS allows for the synthesis of natural peptides which are difficult to
express in bacteria, the inc... 阅读全帖 |
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r*********w
发帖数: 119 | 8 请好心人帮忙！我们学校没有购买这个杂志的access。
多谢！
我的邮箱是：i**********[email protected]
文献见下面（九篇）。多谢啦！
Peptide-Based Therapeutic Approaches for Treatment of the Polyglutamine
Diseases.
Takeuchi T, Popiel HA, Futaki S, Wada K, Nagai Y.
Curr Med Chem. 2014 Feb 17. [Epub ahead of print]
A Translational View of Peptide Treatment of Neurological Disorders.
Brinker T.
Curr Med Chem. 2014 Feb 17. [Epub ahead of print]
Davunetide: Peptide Therapeutic in Neurological Disorders.
Magen I, Gozes I.
Curr Med Chem. 2014 Feb 17. [Epub a... 阅读全帖 |
|
r*********w
发帖数: 119 | 9 请好心人帮忙！我们学校没有购买这个杂志的access。
多谢！
我的邮箱是：i**********[email protected]
文献见下面（九篇）。多谢啦！
Peptide-Based Therapeutic Approaches for Treatment of the Polyglutamine
Diseases.
Takeuchi T, Popiel HA, Futaki S, Wada K, Nagai Y.
Curr Med Chem. 2014 Feb 17. [Epub ahead of print]
A Translational View of Peptide Treatment of Neurological Disorders.
Brinker T.
Curr Med Chem. 2014 Feb 17. [Epub ahead of print]
Davunetide: Peptide Therapeutic in Neurological Disorders.
Magen I, Gozes I.
Curr Med Chem. 2014 Feb 17. [Epub a... 阅读全帖 |
|
l**********1
发帖数: 5204 | 10 Agree with sunny said.
btw, for 肽类analog from mushrom
pls refer
one 2011 review:
by
Wasser SP (2011)
Current findings, future trends, and unsolved problems in studies of
medicinal mushrooms.
Appl Microbiol Biotechnol. 89: 1323-32.
Its PubMed link:
http://www.ncbi.nlm.nih.gov/pubmed/21190105
and for 肽类analog modification to anti-tumor drugs
pls refer,
by
Iwasaki T et al. (2009)
Selective cancer cell cytotoxicity of enantiomeric 9-mer peptides derived
from beetle defensins depends on negatively ... 阅读全帖 |
|
f*******K
发帖数: 34 | 11 在博后期间，合成一种Cys特异性的phosphanate tag，用于Cys Proteome， Cys PTMs
，以及the cross-talk between Cys PTMs and phosphorylation研究应用。方法具有
高度的特异性，高效性，已经多家独立实验室验证。
关于方法意义，引用reviewer comment：
1. To my knowledgy, the CysPAT strategy is the first method to
simultaneously
measure cysteine proteome and protein phosphorylation from the same sample,
which is the major novelty of this study.
相关tag合成方法及应用方法，已经申请美国专利。
文章在MCP发表：http://www.ncbi.nlm.nih.gov/pubmed/27281782
欢迎大家讨论使用。
Abstract:
Cysteine is a rare and conserved ... 阅读全帖 |
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m**********t
发帖数: 385 | 12 有意向的可直接邮件至[email protected]或q*****[email protected] 联系人
：钱小姐,别忘了在邮件里说明信息来源：mitbbs.com或未名人才；
Position: Technical Account Manager:
Opening: 1
Location: US, Piscataway, New Jersey
Job description:
1.Technical Account Manager is the interface between our customers and our
laboratories.
2.Responsible for handling customer inquiries on our products and services
via phone and email.
3.Providing sale quotation and technical support. Managing ongoing projects
and maintaining customer relationships.... 阅读全帖 |
|
m****s
发帖数: 18160 | 13 【 以下文字转载自 Postdoc 讨论区 】
发信人: Chum23 (喜欢春天的人), 信区: Postdoc
标 题: 国内上市企业高薪职位
发信站: BBS 未名空间站 (Tue Mar 4 23:28:49 2014, 美东)
代友转发：诚聘固体制剂研发总监 （职位描述如下）和药物注册专家，有意者请投简
历至Jim Li
-----------------------------------
Process R&D Director of Solid Oral Formulation
Hainan Shuangcheng Pharmaceuticals Co., Ltd. (海南双成药业有限公司)
Location:
China
Contact:
Jim Li
Salary:
80,000.00 - 120,000.00 USD
Type:
Full Time - Experienced
Sector:
Private Industry
Discipline:
Academic / R... 阅读全帖 |
|
t****p
发帖数: 1504 | 14 最近刚参加了一个Cold Spring Harbor的会议，听到一个talk，觉得真是激动人心。特
此跟大家分享一下。
简单的来说，就是质谱能够鉴别磷酸化的peptide，如果分辨率够高，加上定量，那么
就可以看到整个细胞在某种刺激下蛋白磷酸化随着时间的动态变化。
关键词：
定量的实现：假设同时培养两盘细胞，一盘是正常的培养液，一盘含有稳定重同位素的
arginine和lysine。第一盘加EGF，第二盘对照。如果把两盘细胞等量混合，就可以 看
到某个磷酸化peptide的相对变化。
富集：TiO2可以特异地富集磷酸化的peptide。
分辨率和灵敏度：是不是所有可被磷酸化的peptide都可以被检测到呢？由于人们无法
估计总共有多少磷酸化的peptide，所以不知道能cover百分之几。总之大概有10000个
磷酸化的peptide被检测到。
多点磷酸化：也可以看到。甚至是某个位点的磷酸化依赖于另外一个位点，也可能获得
信息。
定位：把蛋白分为胞质蛋白和核蛋白，可以提供蛋白磷酸化后穿梭细胞核的信息。
时间动态：比如加入EGF，处理不同时间。
Cluster：把磷酸化动态变化相似的蛋白 |
|
i*****g
发帖数: 11893 | 15 看了看，好像没有楼主要求的这种peptide kindom database，
楼主本质是要求有个现成的database(not from bioinformatics, but from
functional ones)
现有的所谓protein database，似乎是bioinformatics，一些相对简单的算法，直接从
genome上面生成的，缺乏很多很多posttranslational modification。 这样ms 搜索这
个数据库，匹配法鉴定蛋白，不需要de novo分析出来某个蛋白，大大减少了ms的数据
库工作。这种办法，据说是Scripps John Yates发明的。
现实中，某种功能性的peptide都是一点一点在实验室多少人年 磨出来的。
所以，你的想法注定落空； 现实似乎也没有一个队伍专门做literature screen，归纳
出来一个不断更新的peptide kindom。
这些peptide当然可以归类 到某些蛋白ABC.... 然后你在整个蛋白ABC。。。mapping，
作总结图。这个相信可以找bioinformatics的人搞定，肯定不... 阅读全帖 |
|
k****o
发帖数: 728 | 16 首先，ETD的optimized reaction time是proportional to 1/z2。ETD有利于high
charge state(CS>=+3)。不知道你的peptide大都是多少CS。对于tryptic peptides，
基本都是+2-+3，所以大多CID比ETD效果好。而现在越来越多地用到其他酶解的peptide
比如Lys-C, Glu-C等产生long peptide，那ETD的优势就很明显了。
Fusion用的软件比前几代仪器在data-dependent MS2的设计上有很多优化。你可以针对
不同的CS设定适合各自的CID或ETD（包括ETD的reaction time)。比如+2只用CID，+3和
+4的用both CID and ETD。高于+4的只用ETD。ETD对+3CS可以用60ms，+4就是60 * (3/
4)2= 34ms。其他对于>+3 CS的ETD time以此类推。
最后，不知道你对于MS2是用IT检测（低resolution)还是Orbitrap FT检测（high
resolution)。后者有利于看清long peptid... 阅读全帖 |
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k****o
发帖数: 728 | 17 看你怎么看了。ETD确实是charge assumption的技术。如果想把precursor全部与ETD
reagent anion反应完，则需要比理论optimized reaction更多的时间，而这也同时带
来使First-generation fragment ions继续与reagent ion react产生internal
fragments和更多的small N- and C-term fragment ions。对于不大的peptide（比如
3000 Da以下的），延长reaction time来提高fragment intensity没什么大问题，因为
靠+1, +2 fragments你就能ID或characterize peptide了。 而对于大的peptide，这
样做会产生internal fragmentation（这些internal fragment你是看不到的，他们“
沉淀”成为MS2 background），会让比较small的terminal fragment ions增多（
signal增强），你只能得到比较好的terminal seq... 阅读全帖 |
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R*******1
发帖数: 6 | 18 Ad Description for BS/MS Sr Associate Scientist
Responsibilities:
• Work within the Analytical Protein Chemistry area to provide
analytical support for novel peptide and protein therapeutics in pre-
clinical and clinical development.
• Primary responsibilities include the identification and
characterization of post-translational modifications in protein therapeutic
candidates, using state of the art separations technology and mass
spectrometry instrumentation, with an emphasis ... 阅读全帖 |
|
R*******1
发帖数: 6 | 19 Ad Description for BS/MS Sr Associate Scientist
Responsibilities:
• Work within the Analytical Protein Chemistry area to provide
analytical support for novel peptide and protein therapeutics in pre-
clinical and clinical development.
• Primary responsibilities include the identification and
characterization of post-translational modifications in protein therapeutic
candidates, using state of the art separations technology and mass
spectrometry instrumentation, with an emphasis ... 阅读全帖 |
|
b*****d
发帖数: 61690 | 20 【 以下文字转载自 Biology 讨论区 】
发信人: Dua (Dua), 信区: Biology
标 题: 这人比陆俊更牛了，把自己名字PS到Nature paper上去了
发信站: BBS 未名空间站 (Fri Apr 19 00:16:31 2013, 美东)
新乡医学院药学院院长涉嫌多件学术造假
http://news.sciencenet.cn/htmlnews/2013/4/276959.shtm
上图为陈宙峰在《自然》杂志上发表的论文，下图为疑似被杨俊添加了自己名字的论文。
■本报记者 冯丽妃 唐凤
已发表的论文竟然被“偷”了！近期，美国圣路易斯华盛顿大学麻醉系教授陈宙峰居然
碰到这么一件不可思议的事儿。
一开始收到匿名检举人发来的邮件，陈宙峰还觉得是天方夜谭。然而，事实却让他不得
不信。
匿名检举邮件称，河南新乡医学院药学院现任院长杨俊把陈宙峰曾经发表在《自然》杂
志上的一篇文章“PS”上自己的名字后，附在其简历中，牟取利益。且有确切材料为证。
真有如此剽窃之人？震惊之余，出于好奇，陈宙峰在接下来的一个多月中，利用自己的
空暇时间对杨俊的信息作了些搜集。
在此过程... 阅读全帖 |
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m**********t
发帖数: 385 | 21 以下为该公司最新的岗位需求，请发简历给钱小姐：q*****[email protected]欢迎诸位应聘或推荐身边的朋友，这家公司的待遇还不错，别忘了在邮件里说明信息来源：mitbbs.com或未名人才；
Position: Senior Scientist in New technology
Opening: 2
Location: Nanjing, China
Responsibilities:
1. Technically coordinate all the projects in R&D Dept. Provide valuable
technical input and directions for the department.
2. Supervise the development of new products and technologies to improve the
product pipeline of R&D Dept.
3. Search patents and technical information.
4. Write paten... 阅读全帖 |
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x***a
发帖数: 19 | 22 Dr. Yan received her M.D. from Fujian Medical University in 1983 and
completed Postdoctoral training at the Physicians & Surgeons College of
Columbia University in New York in 1993.
ShiDu Yan was a postdoc at Columbia in 90s. She stated very clearly in her
webpage. She is a very talented and productive researcher. FU Jin was
lucky to have opportunities to work with her therefore get her name included
in those papers.
http://www.pharmtox.pharm.ku.edu/index.php?page=content:faculty
FuJin has ne... 阅读全帖 |
|
g********0
发帖数: 6201 | 23 【 以下文字转载自 Biology 讨论区 】
发信人: Dua (Dua), 信区: Biology
标 题: 这人比陆俊更牛了，把自己名字PS到Nature paper上去了
发信站: BBS 未名空间站 (Fri Apr 19 00:16:31 2013, 美东)
新乡医学院药学院院长涉嫌多件学术造假
http://news.sciencenet.cn/htmlnews/2013/4/276959.shtm
上图为陈宙峰在《自然》杂志上发表的论文，下图为疑似被杨俊添加了自己名字的论文。
■本报记者 冯丽妃 唐凤
已发表的论文竟然被“偷”了！近期，美国圣路易斯华盛顿大学麻醉系教授陈宙峰居然
碰到这么一件不可思议的事儿。
一开始收到匿名检举人发来的邮件，陈宙峰还觉得是天方夜谭。然而，事实却让他不得
不信。
匿名检举邮件称，河南新乡医学院药学院现任院长杨俊把陈宙峰曾经发表在《自然》杂
志上的一篇文章“PS”上自己的名字后，附在其简历中，牟取利益。且有确切材料为证。
真有如此剽窃之人？震惊之余，出于好奇，陈宙峰在接下来的一个多月中，利用自己的
空暇时间对杨俊的信息作了些搜集。
在此过程... 阅读全帖 |
|
w*****t
发帖数: 179 | 24 您好，不知道我的背景能否审到这片文章。我曾经审过一些小杂志，希望能够对我争取
这次机会有所帮助。详细的CV和研究背景如下。非常感谢您的考虑。我的工作邮箱：
z****[email protected]
好像CV在这里粘贴很难看，不好意思。前一段时我的CV，后一段我的较详细的研究经历
。如有需要我更详细的背景情况，请跟我发信。
1
CURRICULUM VITAE
Full Name: Zhimin Wang
Address: Allegheny-Singer Research Institute, West Penn Allegheny, Health
System, South Tower, 320 East North Avenue, Pittsburgh, PA 15212-4772
Phone: 412-359-6153 (Office)
412-737-3777 (Cell)
E-mail: z****[email protected] w************[email protected]
EDUCATION
September, 2001- May, 2005 Ph.D., Molecular M... 阅读全帖 |
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w*****t
发帖数: 179 | 25 hello, thank you for providing the information. Please find my CV below and
my research experience. I have been revewing for over 10 journals for 14
times. My majour of PH.D is microbiology from Wuhan university. I have
published several papers concering virology and bacterial infection. So far
my work also foucse on salivary gland biofilm formation under LL37 and K4s4
treatment. If you need further information, please let me know. My email
address: z****[email protected]
Thank you for your time and c... 阅读全帖 |
|
g*b
发帖数: 60 | 26 Web of Science 上查得 Fu J and Yan SD 所合作的文章。其中 Yan SD 是大多数文章
的通信作者。
要查真相，为何不向这个通信作者写 email 询问呢？
Title: An intracellular protein that binds amyloid-beta peptide and mediates
neurotoxicity in Alzheimer's disease
Author(s): Yan SD; Fu J; Soto C; et al.
Source: NATURE Volume: 389 Issue: 6652 Pages: 689-695 Published: OCT
16 1997
Times Cited: 246 (from All Databases)
[ View abstract ] [ Hide abstract ]
2. Title: Amyloid-beta peptide-receptor for advanced glycation endproduct
interaction elicit... 阅读全帖 |
|
K******S
发帖数: 10109 | 27 as long as the quality of MS2 is high, a peptide match is a peptide match.
But 1 peptide could be matched to different proteins.
if you have 2 or more high quality peptide match for that protein, it's
pretty confident. For those 1 peptide match proteins, you need blast search. |
|
b******y
发帖数: 627 | 28 Short peptides may have low immunogenecity. To increase the chance of
getting Ab, you can synthesize a peptide dendrimer from C TERMINUS to N
terminus (as all peptide synthesis is from C to N): as following:
EK(K(K(linker-your peptide)2)2)2.
In this way, you will have a dendrimer that have 2^n of your peptides
presented outwards. This kind of dendrimer has increased immunogenecity and
therefore higher chance to generate Ab.
Anaspec should have this service although a bit expensive.
good luck. |
|
s******y
发帖数: 28562 | 29 是的，是会有这个问题，不过我们本来是打算用一个空的GST-6Xhis 来预
处理得到的抗血清，把那些和那个peptide 没有关系的东西都清理掉。
不过我就怕万一运气不好，发现把血清处理完之后彻底就没有抗性了
（就是说根本没有抗peptide 的信号）
我们这里还不能直接用(-peptide-)n, 因为我们要求抗体能够识别peptide
N-terminus. 如果用(-peptide-)n 就没有这个性质了。
但是你说的Dentrimer 说不定可以用，因为末端都是暴露的，所以我说不定
会先试试这个。希望不要太贵就好了。呵呵。
lower |
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b******y
发帖数: 627 | 30 It will be expensive from Anaspec. should try luck with smaller company for
this.
Do you have friends who can help you with this? It is not different from
normal peptide synthesis except that the protection on the two -NH2 on the
first Lysines are the same probably fmoc. You also don't mind a mixture.
There is one more strategy if your peptide doesn't have cys in it.
Synthesize your peptide with an extra cys at the C-terminus. Buy tri-
maleimide from somewhere. Mix your peptide with tri-maleimid... 阅读全帖 |
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D*a
发帖数: 6830 | 31 新乡医学院药学院院长涉嫌多件学术造假
http://news.sciencenet.cn/htmlnews/2013/4/276959.shtm
上图为陈宙峰在《自然》杂志上发表的论文，下图为疑似被杨俊添加了自己名字的论文。
■本报记者 冯丽妃 唐凤
已发表的论文竟然被“偷”了！近期，美国圣路易斯华盛顿大学麻醉系教授陈宙峰居然
碰到这么一件不可思议的事儿。
一开始收到匿名检举人发来的邮件，陈宙峰还觉得是天方夜谭。然而，事实却让他不得
不信。
匿名检举邮件称，河南新乡医学院药学院现任院长杨俊把陈宙峰曾经发表在《自然》杂
志上的一篇文章“PS”上自己的名字后，附在其简历中，牟取利益。且有确切材料为证。
真有如此剽窃之人？震惊之余，出于好奇，陈宙峰在接下来的一个多月中，利用自己的
空暇时间对杨俊的信息作了些搜集。
在此过程中，他发现，这位“杨博士”所涉嫌的学术不端行为并非仅此一件……
“真是想人之不敢想，
做前人未做之大事！”
“美国圣路易斯华盛顿大学教授陈宙峰有个有趣的发现。”3月4日，北京大学生命科学
院院长饶毅在发给《中国科学报》记者的一封邮件中称。
这封邮件同时记录了此前饶毅和陈宙峰... 阅读全帖 |
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K******S
发帖数: 10109 | 32 let me put it this way
1st experiment, total ID 5,000, FDR 50% but I got all my lead peptides
verified with either standard peptides or biochemistry/cell biology
experiments. total number of lead peptides ~20
2nd experiment, total ID 5,000 FDR 1% but there's no follow up validation on
lead peptides, total number of lead peptides ~ 200.
If you are about to develop expensive antibody or other assays to translate
the findings into clinic, which experiments you prefer to trust more? You
see my point... 阅读全帖 |
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K******S
发帖数: 10109 | 33 let me put it this way
1st experiment, total ID 5,000, FDR 50% but I got all my lead peptides
verified with either standard peptides or biochemistry/cell biology
experiments. total number of lead peptides ~20
2nd experiment, total ID 5,000 FDR 1% but there's no follow up validation on
lead peptides, total number of lead peptides ~ 200.
If you are about to develop expensive antibody or other assays to translate
the findings into clinic, which experiments you prefer to trust more? You
see my point... 阅读全帖 |
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k****o
发帖数: 728 | 34 不要追求多MS2。如果你只ID到+2,+3的peptide，那原因就在于MS2的resolution低了。
大的peptide（大多+4以上）需要用15,000 resolution Orbitrap扫MS2。
尤其是如果你的酶是Lys-C一类single amino acid residue cleavage的，那主要就
digest出large peptide了。而large peptide对于protein ID的命中率也高于small
peptide。 |
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f*******K
发帖数: 34 | 35 简单解释如下：
做过磷酸化蛋白质组学的应该都知道，TiO2针对磷酸化基团具有很高的亲和性和特异性
，因此TiO2被广泛应用于富集磷酸化肽段的磷酸化蛋白质组研究，以分析磷酸化介导的
信号通路.TiO2富集磷酸化peptide的方法由我博后老板Martin R. Larsen 2005年发表
于MCP,引用有大约1500多次，相关专利被Thermo收购。
在本工作中，我们通过化学合成一种phosphanate tag,该tag可以特异性与Cys的free
thiol group反应，从而用于标记Cys peptide,由于tag含有phosphanate group, 从而
标记的Cys peptide可以用TiO2富集，原理类似于富集磷酸化peptide。更进一步，我们
的方法可以从同一样品同时富集cys peptides和Phosphopeptides, 从而可用于研究两
种PTM的相互作用the cross-talk between Cys PTMs and phosphorylation。 |
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t***n
发帖数: 121 | 36 1. Curr Pharm Biotechnol. 2015;16(11):966-82.
Title : Process Development Strategies in Plant Molecular Farming.
http://www.ncbi.nlm.nih.gov/pubmed/26343135
3. Curr Protein Pept Sci. 2013 Nov;14(7):568-79.
Title : Heterologous production of peptides in plants: fusion proteins and
beyond.
http://www.ncbi.nlm.nih.gov/pubmed/23968345
4. Expert Opin Drug Deliv. 2015 Apr;12(4):653-67. doi: 10.1517/17425247.2015
.974546. Epub 2014 Oct 28.
Title: Elastin-like polypeptide for improved drug delivery for... 阅读全帖 |
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y***l
发帖数: 1095 | 37 嗯，是的只一个三小时的SESSION的话，做PROTEIN 不合适，而且我自己也没有经验，
所以不做那个好了。要不就买A, B, C, D, E五种PEPTIDE，告诉他们是哪五种，一人给
一个未知PEPTIDE MIXTURE，大概有两三种PEPTIDE，然后每人做一个MALDI，用M/Z判断
自己有哪两三种PEPTIDE，不知道这样会不会太简单。。。
还是说不告诉他们A,B,C,D,E是什么，每一个再做MS/MS，然后根据MS/MS自己推是什么
PEPTIDE？或者有什么网上的数据库可以用的？ |
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b*******g
发帖数: 1309 | 38 Carbohydrate-based vaccines are widely used to prevent infectious diseases,
but their use to fight cancer has so far proved problematic. To increase the
chance of success, researchers are pursuing a number of avenues that have a
common goal: enhance carbohydrates’ ability to induce the human immune
system to mount an effective fight against cancer.
Carbohydrates are studded all over the surfaces of human cells, where they
help with essential functions ranging from protein transport and cell-cell... 阅读全帖 |
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v******0
发帖数: 265 | 39 大家好，上次我询问过关于HILIC column的一些问题，得到了不少帮助，但最近我又遇
到了一些问题，实在想不通，只好再来请教。
我打算分离鉴定一些phosphopeptide standard， 我先用C18做了LC-MS,大部分
phosphopeptide都可以看到明显的peak. 一些比较Hydrophilic的似乎peak很小，然后
我就用HILIC（Amide 80 HILIC) 去做,目的是检测这些Hydrophilic，浓度我调的和C18
的那个一样，不同的是C18就是water with 0.1% formic acid, HILIC用的是90%ACN，
10%water,0.1%formic acid. 我是先加了非常少的水（4ul)在sample里，然后又加了
36ul ACN。我sample量不多，所以不敢一上来加很多水去溶解。
我的结果就是用HILIC，几乎看不到什么peak, 理论上HILIC应该比C18要更有利于
phosphopeptide separation,特别是Hydrophilic的，但我却只能看到很小的几个peak,
大部分都消失了，而且也... 阅读全帖 |
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i**********d
发帖数: 853 | 40 谢谢回复，我之所以问这个问题是因为：从外来抗原被注射入体内，到APC细胞把它和
MHC II的complex表达在细胞膜上之间有一些复杂的步骤，我个人认为这中间的过程是
解释这个题的关键所在。
基本步骤：
1. Exogenous antigens, such as viruses, are engulfed and placed in a
phagosome.
2. Lysosomes fuse with the phagosome forming a phagolysosome.
3. Protein antigens are degraded into a series of peptides.
4. MHC-II molecules are randomly synthesized in the endoplasmic reticulum;
since MHC is very complicated system and can form numerous different MHC II
molecules, in most time at least one MHC II ... 阅读全帖 |
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k****z
发帖数: 1863 | 41 Yan, SD, Yan, S-F., Chen, X., Fu, J., Chen, M., Kuppusamy, P., Smith, M.A.,
Perry., G., Godman,G.C., Nawroth, P., Zweier, J.L., and Stern, D. Non-
enzymatically glycated tau in Alzheimer’s disease induces neuronal oxidant
stress resulting in cytokine gene expression and release of amyloid ß-
peptide. Nature Medicine, 1(7):693-699, 1995
Yan SD, Chen X, fu J, Chen M, Zhu H, Roher A, Slattery T, Nagashima M,
Morser J, Migheli A, Nawroth P, Goodmand G, Stern D, and Schmidt AM. RAGE in
Alzheimer... 阅读全帖 |
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k****z
发帖数: 1863 | 42 他妈怎么把它生出来的？
Yan, SD, Yan, S-F., Chen, X., Fu, J., Chen, M., Kuppusamy, P., Smith, M.A.,
Perry., G., Godman,G.C., Nawroth, P., Zweier, J.L., and Stern, D. Non-
enzymatically glycated tau in Alzheimer’s disease induces neuronal oxidant
stress resulting in cytokine gene expression and release of amyloid ß-
peptide. Nature Medicine, 1(7):693-699, 1995
Yan SD, Chen X, fu J, Chen M, Zhu H, Roher A, Slattery T, Nagashima M,
Morser J, Migheli A, Nawroth P, Goodmand G, Stern D, and Schmidt AM. RAGE ... 阅读全帖 |
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k****z
发帖数: 1863 | 43 发信人: pangduer (白飞飞), 信区: WaterWorld
标 题: Re: 傅瑾教授：已经把在哥大的学习经历说明和学位证书复印件提
发信站: BBS 未名空间站 (Mon Jul 23 14:03:45 2012, 美东)
对，这篇1997年的nature paper，列在第一位的yan shidu其实是唯一的通讯作者，列
在最后一位的本该是通讯作者的David Stern其实就是当时部门里的大牛。估计情况就
是David Stern是大老板，但是对这个project其实没啥贡献，然后是作为通讯作者的
Yan shidu是小老板，带领Fu Jin等一干人做出来的，才会有这样奇怪的authorship。
这样看来，Fu JIn实质上是真正干活的第一作者，凭这样一篇nature paper获得博士
也就没啥稀奇的了。Fu Jin是1999年拿的博士，1997年的paper正好对景。
凭什么方肘子要看学位证明人家就亮给这个傻逼看啊。他家菊花不是都拒绝出示学位证
明吗？人家Fu Jin教授把学位亮给学校看就好了，气死方教主及其教徒们！
Yan, SD, Yan, S-F., C... 阅读全帖 |
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k****z
发帖数: 1863 | 44 Yan, SD, Yan, S-F., Chen, X., Fu, J., Chen, M., Kuppusamy, P., Smith, M.A.,
Perry., G., Godman,G.C., Nawroth, P., Zweier, J.L., and Stern, D. Non-
enzymatically glycated tau in Alzheimer’s disease induces neuronal oxidant
stress resulting in cytokine gene expression and release of amyloid ß-
peptide. Nature Medicine, 1(7):693-699, 1995
Yan SD, Chen X, fu J, Chen M, Zhu H, Roher A, Slattery T, Nagashima M,
Morser J, Migheli A, Nawroth P, Goodmand G, Stern D, and Schmidt AM. RAGE in
Alzheimer... 阅读全帖 |
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a*******s
发帖数: 117 | 45 以下为部分论文。 若有错误，欢迎指正。
夏宁邵所在领域还是偏应用，非基础研究。 亮点是2010年的一篇柳叶刀。
2012年度：
Quantitative hepatitis B core antibody level may help predict treatment
response in chronic hepatitis B patients.
Yuan Q, Song LW, Liu CJ, Li Z, Liu PG, Huang CH, Yan Y, Ge SX, Wang YB, Peng
CY, Zhang J, Kao JH, Chen DS, Chen PJ, Xia NS.
Gut. 2012 Jun 14. [Epub ahead of print] No abstract available.
影响因子： 10.614
Hepatitis E virus: neutralizing sites, diagnosis, and protective immunity.
Zhang J, Li SW, Wu T, Zhao Q, Ng MH, Xia NS.
... 阅读全帖 |
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j*****y
发帖数: 121 | 46 Novo Nordisk R&D Center China
Mini-pilot – Senior/Principal Scientist in DSP
Zhongguancun Life Science Park, Beijing
Novo Nordisk is a focused healthcare company and a world leader in diabetes
care. Novo Nordisk China R&D (NNRCC) is an integrated part of Novo Nordisk R
&D organization. Novo Nordisk wants to conduct pharmaceutical research
activities in China and expand its network to the scientific community. For
this purpose a new position is open for a dedicated and motivated Senior
Scientist/... 阅读全帖 |
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x**********7
发帖数: 973 | 47 【 以下文字转载自 Pharmaceutical 讨论区 】
发信人: xiaojiang007 (夜叉巡海), 信区: Pharmaceutical
标 题: 我们公司招一个初级职位，做PD /formulation
发信站: BBS 未名空间站 (Fri Aug 26 21:53:45 2016, 美东)
我们组招一个本科硕士级别的 PD associate scientist
限定本科硕士学位 （这是老板的决定）
有同胞愿意来，请email 我你的简历和coverletter （下面可能要正式贴广告）
先说一下这个职位优缺点让大家有个准备：
缺点： 公司小，原本是CMO，低级职位，工资不高，所在小城市偏僻 （GA一个极小城
市）
优点： 做的方向不错，兼做formulation和proccess development，了解的自然知道
PD比RD好得多。公司可以支持H-1B（自己抽签），公司正在赚钱扩张
Job description
Responsibilities:
This entry level position will focus on process and fo... 阅读全帖 |
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j*****y
发帖数: 121 | 48 Novo Nordisk R&D Center China
Mini-pilot – Senior/Principal Scientist in DSP
Zhongguancun Life Science Park, Beijing
Novo Nordisk is a focused healthcare company and a world leader in diabetes
care. Novo Nordisk China R&D (NNRCC) is an integrated part of Novo Nordisk R
&D organization. Novo Nordisk wants to conduct pharmaceutical research
activities in China and expand its network to the scientific community. For
this purpose a new position is open for a dedicated and motivated Senior
Scientist/... 阅读全帖 |
|
发帖数: 1 | 49 As a thriving pharmaceutical company, Protheragen Inc. ("Protheragen")
focuses on acquiring, developing and commercializing novel pharmaceutical
and biotechnology products for both therapeutic purposes and diagnostic
applications. Guided by our advanced business strategy – the “VIC R&D
model”: V (risk investment), I (IP right) and C (CRO), we are dedicated to
making drug discovery and development activities the most effective via a
collection of efforts and advantages from all agencies.
We are p... 阅读全帖 |
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x***a
发帖数: 19 | 50 Check this website:
http://www.pharmtox.pharm.ku.edu/index.php?page=content:faculty
Shirley ShiDu Yan
Howard Mossberg
Distinguished Professor
(785) 864-3637
s******[email protected]
Find out more about the Yan Lab.
Dr. Yan received her M.D. from Fujian Medical University in 1983 and
completed Postdoctoral training at the Physicians & Surgeons College of
Columbia University in New York in 1993.
ChSelected Recent Publications
Yan, SD, Yan, S-F., Chen, X., Fu, J., Chen, M., Kuppusamy, P., Smith, M.A.,
Perry... 阅读全帖 |
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