i********n
发帖数: 171 | 1 有审稿意愿的请发送你的CV到[email protected]/* */并告知你希望审稿的文章编号
请有时间愿意多审几篇的也可以，只要你能保证质量，之前有些审稿人有些敷衍，希望
大家能认真对待。谢谢合作。
1. Comparative assessment of cytotoxic effect of Cuban and Brazilian
propolis in human laryngeal epidermoid carcinoma cells
2. Role of PI3K/AKT/mTOR signaling pathway in ostearthritis by regulating
articular chondrocytes autophagy in rats
3. CREB knockdown inhibits growth and induces apoptosis in human pre-B
acute lymphoblastic leukemia cells through inhibition of prosurvival sig... 阅读全帖 |
|
s****h
发帖数: 31 | 2 由于老婆工作变动，１０月分全家搬到RTP (孩子太小，全家得一块搬)。最近开始找工
作，不得要领。借贵宝地问一下，有熟悉情况的大牛给支招。
生物化工科班，在美国一中等规模制药厂呆了５年，主要从事 Analytical
biochemistry里的Protein Characterization (mAbs, vaccine, peptides) 工作。熟
悉peptide map, intact mass, LC-MS/MS, ICP等相关技能。站内回信，谢谢各位了。 |
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j*******s
发帖数: 155 | 3 Our company is hiring a biostatistician. Support H1-B visa and green card
application. Please see below job description. Contact me via xxie@caprion.
com if you are interested.
Caprion Proteomics US LLC is seeking a highly motivated individual with
experience in bioinformatics and statistical analysis in a collaborative,
proteomics focused scientific environment at its site in Menlo Park,
California. This position will interact remotely with the main
Bioinformatics group situated in Montreal, Ca... 阅读全帖 |
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d*****u
发帖数: 28 | 4 Interested in both academic and industry job.
Will graduate this December
Need a job close to NIH, (fc is working there)
Some experience:
ELISA
HPLC for peptide purification
Phage display (peptide libraries)
Western blot
two D
optical imaging-fluoresence imaging
nulear medicine( Indium labelling)
PCR
transformation
cell culture
cell binding assay
Background
molecular imaging in cancer
Thanks a lot if you can tell me some job opportunities. |
|
B******1
发帖数: 9094 | 5 发信人: cyberholic (cyberholic), 信区: Chemistry
标 题: Re: 我这五年的事
发信站: BBS 未名空间站 (Tue Dec 25 14:18:06 2012, 美东)
当时组里唯一一个亚洲人就是一个四年级的日本师兄，很典型的那种让你记不住的日文
音传写（不知道他的汉字名字）。我们接触的次数实在有限，我现在对他的印象就是长
头发，戴眼镜，然后就是他的抑郁与忧愁。我最后一次见到他，是在一天晚上，他在吃
饼干，心不在焉地翻着那本strategic application of named reactions的书。他和我
说，他已经决定quit phd回日本了。也许是见我当时面有惊恐之色，他接着说，Steve
还是一个不错的老板，我决定quit了，他也很尊重我的决定，还给我写了推荐信。。。
于是很快，他就消失了，我便继承了这个师兄的hood，bench，和desk。我曾经试图了
解到底发生了什么，结果发现，他在人们的记忆中消失的很快，正如他的名字在网页上
消失一样。我听说的是，他因为全组从B大学搬到了A大学，到了一个新地方不适应，所
以抑郁了。一个... 阅读全帖 |
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P*******e
发帖数: 39399 | 6 ☆─────────────────────────────────────☆
BigBlue (#15) 于 (Tue Feb 26 19:23:34 2013, 美东) 提到:
发信人: BigBlue (#15), 信区: USANews
标 题: 49ers和29ers: 奥巴马全民保险两个立竿见影的影响
发信站: BBS 未名空间站 (Tue Feb 26 19:14:22 2013, 美东)
法案其中有个规定, 凡是雇佣50个或更多全职员工的企业,
如果不给每一个人都提供医疗保险, 就从第30个开始, 每人每年罚$2000.
而"全职"的定义, 是一个人每周工作30个小时. 注意是30, 不是40.
更荒谬的是, 如果有两个人各工作15小时, 那也算一个"全职".
法案是2014年生效, 但是采集数据 --
公司总共有多少个员工, , 其中多少个算全职, 等等 -- 现在已经开始了.
所以, 那些为全民保险高声欢呼的自由派左棍们,
你们要是还有一点现实感的,
要还不是完全沉浸在理想化的自由主义世界里痴人梦呓的,
也应该猜得到在现实世界里, 正在发生什么事情了罢:... 阅读全帖 |
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r**z
发帖数: 1793 | 7 看到第三篇 又是不同结论
Diabetologia. 2011 Feb;54(2):320-8. Epub 2010 Nov 3.
Associations between the intake of caffeinated and decaffeinated coffee and
measures of insulin sensitivity and beta cell function.
Loopstra-Masters RC, Liese AD, Haffner SM, Wagenknecht LE, Hanley AJ.
SourceDepartment of Nutritional Sciences, Faculty of Medicine, University of
Toronto, FitzGerald Building, 150 College St, Toronto, ON, Canada.
Abstract
AIMS/HYPOTHESIS: Although protective relationships between coffee
consumption a... 阅读全帖 |
|
i*********5
发帖数: 19210 | 8 刚才听podcast听到的，挺好玩。
（下面的链接可以在线播放）
http://soundcloud.com/the-peptides/the-peptides-attack-of-the
SHOELACES ARE ALL TIED UP
HAIR COMBED COFFEE IN A CUP
BRIGHT & EARLY OUT OF LUCK
HURLY BURLY TOPSY TURVY
TOTEM MOTOR INN MOTEL
PURGATORY LIMBO HELL
TREADMILL RACE MARATHON PACE
UPHILL RUN DOWNHILL CHASE
BODY DON'T YOU LOSE THE WILL
DON'T YOU LOSE THE WILL TO LIVE
GRAVITY WILL ULTIMATELY
PERMANENTLY KILL ME
VELOCITY WILL ABSOLUTELY
POSITIVELY KILL ME
GRAVITY VELOCITY
WILL DEFINITELY OBVIOUSLY
EVIDENTLY ... 阅读全帖 |
|
r********m
发帖数: 593 | 9 这楼包括Kanebo, Kaplan MD, Kate Someville, Kiels
===Kanebo===
很不错的一个品牌, 她家的产品十分适合中国人的皮肤
Sensai 系列, 除非特别注明, 小样均为管装
Wrinkle Repair Cream
112G
Size 8mL
Value 48.00
Price 8.00
Wrinkle Repair Essence
12G
Size 7mL
Value 35.00
Price 5.00
Prime Solution
1XC
Size 10mL
Value 23.00
Price 4.00
Hydrachange Mask
113A3
Size 15mL
Value 24.00
Price 3.00
Silky Purifying Cleansing Oil
1XB
Size 30mL
Price 1.00
Silk Peeling Mask pack
Size 3mL
Qty 2
Get one free with... 阅读全帖 |
|
r********m
发帖数: 593 | 10 接着上一楼各种杂牌东东...
=== Lab series === 图1
Age rescue water-charged gel cream tube
A74
Size 15mL
Value 15.00
Price 1.00
Buy this, get all 4 samples free
*** Free samples ***
Lab series Age rescue face lotion
Size 2mL
Lab series Age rescue eye therapy
Size 1.5mL
Lab series Daily moisture defense lotion SPF 15
Size 2mL
Lab series Daily moisture defense eye balm
Size 2mL
**********************************************************
===Lancer=== ... 阅读全帖 |
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n*****w
发帖数: 1325 | 11 ☆─────────────────────────────────────☆
ilovesam (感谢CCTV, MTV... i love ya all) 于 (Thu Jul 11 13:19:12 2013, 美东) 提到:
暗算的三部，黎明之前，潜伏，这三部颠峰之作算第一天团，还有什么？
☆─────────────────────────────────────☆
maywind (五月风) 于 (Thu Jul 11 13:30:38 2013, 美东) 提到:
还有誓言无声和誓言永恒
☆─────────────────────────────────────☆
yueru (月如) 于 (Thu Jul 11 14:06:33 2013, 美东) 提到:
有没有高清的版本？
☆─────────────────────────────────────☆
maywind (五月风) 于 (Thu Jul 11 14:17:46 2013, 美东) 提到:
请txbb同学
或者去youku，sohutv 什么的找找
我看电视要求不... 阅读全帖 |
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t******k
发帖数: 553 | 12 我不发表对二人是否结婚事情的任何意见，也不想知道，这个世界为博出位出名，手段多了去了。
裴院士，以前听过报告，有所认识。对于谢欣，这位最年轻的973首席科学家，海龟博士。开红色Audi TT的973兽吸，我还是第一次听说，无聊间，google了一下。
以下是转帖
引用:
这里就是一个学术造诣颇深的院士和一个混迹学术界想要出头的高干千金的两厢情愿的合作。
我们先来看一下一个手里控制着上亿元科研经费的中国最年轻的973首席科学家，973胚
胎发育干细胞项目首席科学家的基本情况：
谢欣研究员， 现任中国财政部长谢旭人之女
现任 中国科学院上海药物所 研究员、 国家新药筛选中心模型建立 II 部主管、 课题
组长 。
下面是她所有的有名字的文章，还算诚实，没用同名同姓的凑数。
可是大家已经要问这样的条件，没有一篇CNS系列的文章，1st-authored paper只有3篇
，够中科院研究员、百人计划择优资助吗？
那些录用的评委和百人计划的专家组，都糊涂了吗？这么一反以“严格”著称之常态了
呢？
1.Chen D, Liao J, Li N, Zhou C, Liu Q, Wang G, Zh... 阅读全帖 |
|
a**********e
发帖数: 418 | 13 http://en.wikipedia.org/wiki/Peptide_bond
A peptide bond can be broken by amide hydrolysis (the adding of water). The
peptide bonds in proteins are metastable, meaning that in the presence of
water they will break spontaneously, releasing 2-4 kcal/mol [1] of free
energy, but this process is extremely slow.
consider the equilibrium reaction at rt: dipeptide + H2O ---2 amino acids
The free energy difference is 2-4 K/Cal, I am taking the average 3, which
correspond to a greater than 99% of the prod... 阅读全帖 |
|
l**********t
发帖数: 5754 | 14
And that energy barrier(to break a peptide) is lower than the one to
form a peptide. |
|
G****e
发帖数: 11198 | 15 introduction:
http://chem.berkeley.edu/people/faculty/xu/xu.html
group page:
http://www.mse.berkeley.edu/groups/xu/index.htm
Research Interests: Polymers, Biomaterials, Materials Chemistry
The key to future technology is the design and fabrication of functional materials having structures ordered down to the molecular level. This goal can't be reached by conventional "top-down" approaches and remains as the "holy grail" using synthetic materials such as block copolymers. In addition, there have ... 阅读全帖 |
|
B**T
发帖数: 294 | 16 祝贺！
materials having structures ordered down to the molecular level. This goal
can't be reached by conventional "top-down" approaches and remains as the "
holy grail" using synthetic materials such as block copolymers. In addition,
there have been limitations in the functionalization of these
nanostructured materials. On the other hand, nature has made a dizzying
array of materials using a few building blocks. Remarkable progre: ss has
been made in de novo protein design and various peptides ha... 阅读全帖 |
|
T****n
发帖数: 2195 | 17 06年出去那个MIT的不知道怎么样了。
materials having structures ordered down to the molecular level. This goal
can't be reached by conventional "top-down" approaches and remains as the "
holy grail" using synthetic materials such as block copolymers. In addition,
there have been limitations in the functionalization of these
nanostructured materials. On the other hand, nature has made a dizzying
array of materials using a few building blocks. Remarkable progre: ss has
been made in de novo protein design and var... 阅读全帖 |
|
m*********e
发帖数: 216 | 18 不是大工的，也来凑个热闹，祝贺一下。
再批评一句：不就是个伯克利的aP（小写的a，不是大写的A），至于吗？让这个藤那个
藤的AP， P们情何以堪？
materials having structures ordered down to the molecular level. This goal
can't be reached by conventional "top-down" approaches and remains as the "
holy grail" using synthetic materials such as block copolymers. In addition,
there have been limitations in the functionalization of these
nanostructured materials. On the other hand, nature has made a dizzying
array of materials using a few building blocks. Remarkable prog... 阅读全帖 |
|
c********n
发帖数: 1065 | 19 你们怎么不去看看这个姐姐的老公是谁
materials having structures ordered down to the molecular level. This goal
can't be reached by conventional "top-down" approaches and remains as the "
holy grail" using synthetic materials such as block copolymers. In addition,
there have been limitations in the functionalization of these
nanostructured materials. On the other hand, nature has made a dizzying
array of materials using a few building blocks. Remarkable progre: ss has
been made in de novo protein design and vario... 阅读全帖 |
|
m*********e
发帖数: 216 | 20 不是大工的，也来凑个热闹，祝贺一下。
再批评一句：不就是个伯克利的aP（小写的a，不是大写的A），至于吗？让这个藤那个
藤的AP， P们情何以堪？
materials having structures ordered down to the molecular level. This goal
can't be reached by conventional "top-down" approaches and remains as the "
holy grail" using synthetic materials such as block copolymers. In addition,
there have been limitations in the functionalization of these
nanostructured materials. On the other hand, nature has made a dizzying
array of materials using a few building blocks. Remarkable prog... 阅读全帖 |
|
c********n
发帖数: 1065 | 21 你们怎么不去看看这个姐姐的老公是谁
materials having structures ordered down to the molecular level. This goal
can't be reached by conventional "top-down" approaches and remains as the "
holy grail" using synthetic materials such as block copolymers. In addition,
there have been limitations in the functionalization of these
nanostructured materials. On the other hand, nature has made a dizzying
array of materials using a few building blocks. Remarkable progre: ss has
been made in de novo protein design and vario... 阅读全帖 |
|
R********a
发帖数: 467 | 22 这些peptide的东西，其实都可以靠recombinant DNA的办法来生产。就是本来２１世纪
的技术。问题是好象老美对生物改基因制造的东西都特别反感。所以这个凡是有天然提
取的，基本生物没有市场。参考humulin。
生物的这个rDNA，是无数生物学前人用泪水和血换来的。里面每一步都有诺贝尔奖。都
是人类对自然认识的一个突破。那些生物的创造，智慧，刻苦，真的非常让人尊敬。当
然，目前混普通学校的老中根本是两码事。
目前比较成功的是一个叫regn的公司。他们就更进一步了。是protein design，然后生
产。类似EE的芯片。人家公司的一个CXO，是美国科学院／医学院两院院士，哥大校友
。介绍起身平都是纽约州长大的。纽约州外号empire state。的确是出世界级人才的地
方。类似上海和周边地区。
然后，合成也是一种方法。上次那个来推销国内高中的，估计就是做合成peptide的。
问题是那是一条死路。目前基本没有工业化的实战案例。里面的技术问题成堆。每年
AICHE都有讨论，都有paper。你要是做OPRD的reviewer，那是老能受到。问题是那么多
年了，还是没有解决。 |
|
发帖数: 1 | 23 ◇◇新语丝(www.xys.org)(newxys.com)(xys10.dxiong.com)◇◇
举报南京大学生命学院院长华子春学术不端
方先生您好！
我们反映南京大学生命学院院长、长江、杰青、 国家科技进步奖获得者、
国重主任华子春长期存在学术不端问题。 附件是我们发现的第一批四组共9篇文
章。
1. 2000-C和2000-E两篇文章皆为英文稿， 分布发表于南京大学学报和
Protein Expression and Purification。华教授是通讯作者。 南京大学学报的
内容系于后者的文字上做了删掉，无文字改写。 例如：
前者的第一、二段：
Human cardiac-specific homeobox protein (hCsx2 or Nkx2.5) encodes
a homeobox transcription factor of 323 amino acids containing six
cysteines and is composed of three domains: the TN-domain, the
homeobox- domain, and t... 阅读全帖 |
|
A***l
发帖数: 302 | 24 CURRENT PROBLEMS AND THE APPROACHES TO RESOLVE IT
More than 37 years ago, protein fragments of tobacco mosaic virus were
proven to elicit antibodies that neutralized the whole virus (7). Today
there are still few, if any, synthetic peptide-based vaccines commercially
available. There are a number of contributing reasons for this, among
which are the facts that: (1) the peptide sequences used to represent
epitopes are generally short and as a consequence contain insufficient
information to fold i |
|
A***l
发帖数: 302 | 25 2. Adjuvant approach
Since a single synthetic peptide is usually not enough to induce an
immunogenic response, they often require an adjuvant. Adjuvants are
certain substances which have the ability to selectively modulate the immune
response to elicit humoral/cellular immune responses (18). An MHC class I
response usually appears in response to intracellular pathogens such as
virus, leading to CTLs (cytotoxic T lymphocytes). Such response is usually
not observed with single peptide antigens. Ad |
|
A***l
发帖数: 302 | 26 3. Linear or branched covalently attached T- and B-cell epitopes (Gamete
antigens).
As we have mentioned, short synthetic peptides representing B-cell
epitopes are incapable of inducing B-cells to differentiate and produce
antibody unless associated with epitopes that are recognized by helper
T-cells. The early study of the immune response to synthetic antigens has
shown that uncoupled peptides can realize their potential as vaccines if
they contain domains that react with helper T-cell receptor |
|
A***l
发帖数: 302 | 27 . Discussion and conclusion
These four approaches are among the most popular approaches in the design of
synthetic peptide vaccine. Of course, they are not incompatible with each
other. For example, synthetic peptide combinatorial libraries offer a
convenient way to find epitopes, which are basic components of Gamete
antigens and MAPs. The two-epitope MAP could be looked as highly branched
covalently attached T- and B-cell epitopes. Adjuvants have been connected to
MAP to make more powerful mala |
|
y***n
发帖数: 99 | 28
================
this is not right.
pI determined by the 2D gel system, the time you cut out the spot.
quatuple mass spec can actually sequence some partial sequence,
which is easy part. Hard part is that you cann't get enough protein
when go to the quatuple, Electrospray requires ten teims more protein
than MALDI.
In-gel digestion coupled by MALDI can give the masses of digested peptide.
use those peptide mass, there are websites you can search the potential
protein candidate with predited m |
|
g***m
发帖数: 465 | 29 hehe, it is a basic strategy via accurate MS for proteomic level's screening
and identifying.
The precision is really dependant on how you construct the peptide map
database. Generally, they take cDNA translated amino acid sequence as actual
proteins, then do a in silica proteolysis with trypsin, input the results into
the database.
This approach is really database dominating. Not mention possible
post-translational modification and signal peptides and so on that could bring
a big trouble. |
|
r****o
发帖数: 105 | 30 你给的信息不够，至少得说说这两个蛋白的标签是什么吧，比如
Flag, HA, 或者myc什么的。既然你用proteinA/G，你应该还加了一种
抗体可以把两个蛋白中的一个拽下来吧？
背景高倒是很常见，因为非特异性的结合会比较严重。要做得好，
被首先拽下来的蛋白的tag要比较好才行，而且如果用多次串联的TAG
会比较好，比如6XFLAG。也有人用两种不同的TAG串联在一起，通过
两步纯化来增强特异性。另外就是最好直接用抗体共价耦联的琼脂凝胶(
比较贵，1ML可能要五百多刀)。
还有就是，一般的做法是直接把洗过的beads 直接用sample buffer
煮。这样的样品非特异性的成分会不少，但如果用TAG peptide的溶液
冲洗蛋白(比如anti-flag matrix 用flag peptide 来冲洗)，效果会好很多。
没信号也许是因为缓冲液太强，或者是因为两个蛋白结合太弱，
冲洗过程中脱落了。用crosslinker可能会有所帮助。 |
|
s*******e
发帖数: 740 | 31 If you clone a 30 bp DNA into the 5' of the filamentus phage
gene III or VIII, it will express a 10 Aa peptide on the surface
of the phage partical. So if you have a libray of random sequence
30bp DNA, you're able to get a libray of phage with each of
them express a different random 10 Aa peptide on the surface.
That is a 10Aa phage display library.
If you have a ligand and you're able to immoblize it on the coloumn,
you can screen the libray to see which of them bind.Sequence the binding
phage |
|
h******n
发帖数: 493 | 32 If you take a pY peptide and try this, you will not be able to
see the loss of phosphate group easily. The phosphate group on
the Tyr residue is really stable. However, this phenomenon is
true for pT and pS peptides because they are much less stable.
Mass Spec is really powerful, but it can't do everything, even
though I am a MS person and I really hope it is that powerful.:) |
|
G***G
发帖数: 16778 | 33 thanks. I used OMSSA before. did you use it?
do you know how to get peptide quantition from OMSSA?
is it easy to get peptide/protein quantition from x!tandem? |
|
p*****r
发帖数: 64 | 34 最近想做果蝇的抗体，antigen是不是用peptide也可以，还是在果蝇里peptide效果不
好，要用蛋白的？ 多谢了 |
|
g*****y
发帖数: 6325 | 35 不要只看你的insert 的基因序列，仔细看看你insert的基因前面有没有ATG. 如果有，
可能翻译起始位点都不一样。 我有一次从别人那里拿了一个vector. 把我的基因插到
mcs里，后来发现表达不对，再来查原基因，原来mcs之前还有一个signal peptide. 而
且不在一个frame里，于是又作克隆去除那个signal peptide才搞对。 |
|
s******y
发帖数: 28562 | 36 那么目前用多肽做出来的aptamer, 最高结合力有多少？
能不能给我推荐几个peptide aptamer and protein target pair? 最好是结合力比较
高一点的，而且靶蛋白本身是可溶的（最好不要是膜蛋白）。
我对这个一直有兴趣，很想知道有什么已知的 peptide aptamer kd <10E-8 的？
先谢谢了！
receptors
each
interaction |
|
s******y
发帖数: 28562 | 37 Thanks!
The reason people are interested in RGD peptide is because it mimics the
extracellular matrix and binds to the integrins, I believe.
I actually don't really care what kind of peptide it is. However I am kind
of planning to do some pilot experiments by myself and I prefer to use
something that can be handled easily. Therefore, I would like to have
something that is sovlable and cytoplasmic (which will be easy for
expression and require minimal posttranslational modification). While the
ex |
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w******e
发帖数: 1187 | 38 so you mean the streptavidin beads only bind to 80% of the biotinylated
peptide you put in? that's actually lower than I thought hehe. How
did you get the result? measure the peptide concentration in supernatant
b4 & after incubation? could u pls let me know which kit you use?
Thanks for the advice. I'll probably try the kit for measuring labeling
efficiency:)
streptavidin |
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s*********y
发帖数: 387 | 39 Thanks for the infor.
Results as below from Google Scholar
the one you mentioned for methodology is 4k citation comparable to
XiaoDong's case.
"
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and
analysis by gel electrophoresis.
jbc.org [PDF]… , SG Fischer, MW Kirschner, UK Laemmli - Journal of
Biological …, 1977 - ASBMB
THE JOURNAL OF Bm.oorc~~ CHEMISTRY Vol. 252, No. 3, Issue of February
10, pp.
1102-1106, 1971 Prrnfed m USA. ... Peptide Mapping by Limited
Proteolysis in Sodi |
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c******r
发帖数: 3778 | 40 有几种情况：
1. 上面sunny同学说的很对。有alternative splicing等其他可能。
2. ko一般都是ko一两个exons，一般assume不完整的RNA或者peptide不稳定，会很快被
代谢掉。但是事实上经常不是这样。有很多蛋白即使ko掉几个exons后还有detectable
的peptide，虽然size shift了，但是确实western可以detect。
3. 如果是conditional ko就有ko效率，和部位，细胞种类等其他原因导致在一定程度
上可以detectable。
不论怎样，要证明ko都要从几个方面来证明：
1. 从genomic上，要跑southern，看到size减小as expected。
2. 从RNA上，northern，和western证明没有detectable的stable的蛋白。如果有，要
证明这个蛋白的功能被comprise了。
3. 从功能上证明这个蛋白的功能没有了。
4. 从phenotype上证明这个功能和这个phenotype直接的联系。 |
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n***3
发帖数: 663 | 41 Immunol Rev. 1995 Jun;145:5-31.
Liposomes as carriers of peptide antigens: induction of antibodies and
cytotoxic T lymphocytes to conjugated and unconjugated peptides.
Alving CR, Koulchin V, Glenn GM, Rao M.
please send the paper to e********[email protected] |
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n***3
发帖数: 663 | 42 Immunol Rev. 1995 Jun;145:5-31.
Liposomes as carriers of peptide antigens: induction of antibodies and
cytotoxic T lymphocytes to conjugated and unconjugated peptides.
Alving CR, Koulchin V, Glenn GM, Rao M.
please send the paper to e********[email protected] |
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w******e
发帖数: 1187 | 43 这点还挺counter intuitive的。不知道他们用什么formulation。如果是NP，liver应该
是个主要的目的地（所以人们才质疑RNAi在肝脏以外能否work）。如果只是peptide，
renal clearance应该很厉害吧。and居然能过BBB，不知道有没有加其他料。印象中
有某peptide能过BBB但不记得是什么了，也不记得有没有paper跟进。
我个人理解medical imaging是否成功，一个是signaling motif要NB：无毒，yield高，
不受autofluorescence影响，穿透性好，etc；一个是targeting motif要NB：足够特异，
target level够高，最好affinity还能恰到好处，half life能控制好。这两方面我觉得
都不容易解决吧。fluorescence在background、穿透性等方面有局限吧？当然这方面如果
tsien组估计最有实力解决。target方面validated biomarker忒少了，
用于imaging，只有一层specificity check也有点安全隐患。
最后... 阅读全帖 |
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l***m
发帖数: 26 | 44 没peptide么？这两年投资这么猛。那个staple peptide的公司不是1b被收购了么么？ |
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f**u
发帖数: 346 | 45 现在通常是两种做法，一是你自己表达蛋白，纯化出来寄给公司让他们给你打。
二是你让公司合成一段很短得peptide然后用他帮你免疫兔子。
好像你可以把你的蛋白全长寄给他们然后他们会分析一下然后给你推荐一段作为抗原。
当然你也可以自己分析，或者自己指定peptide的序列。
了？ |
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c********d
发帖数: 118 | 47 我用了 light, medium, heavy三种 K/R标记细胞中的蛋白
药物处理以后检测磷酸化蛋白的变化水平
有一些磷酸化peptide的ratio比如heavy:light=15
但是，当仔细看这个peptide的mass spectrum时，他们的峰面积or峰高的差别只有4,5
倍的样子。 |
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K******S
发帖数: 10109 | 48 of course it can with labeling, label free is also possible for same peptide
across samples. you can't compare different peptides though. |
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X******n
发帖数: 914 | 49 Protein precipitation is basically the removal of proteins from solution.
This can be achieved in many ways including the addition salt, which takes
away the water molecules surrounding proteins in solution (as the salt
concentration increases, more water molecules associate with the salt ions
instead of the proteins) and as a result the protein molecules come together
since less water is present between them. From this simple definition you
can generalize that protein precipitation does not imp... 阅读全帖 |
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