n********k
发帖数: 2818 | 1 As you folks are discussing Cori, I happened to read his autobiography and
find it both interesting and enlightening:)))...BTW, it sounds Richard Axel
is a lot nicer than what I/we have heard...or in another word, his cruelty
is rooted in his obsession with science and indifference to humanity:))); As
well, it is pretty...he noted Cori but left his ex out...pretty human or
not human?
Autobiography
Richard AxelNew York City is my world. I was born in Brooklyn, the first
child of immigrant parents... 阅读全帖 |
|
E**********1
发帖数: 73 | 2 做蛋白蛋白interaction.蛋白A MW 255KD, 蛋白B MW 58KD，蛋白C MW 229KD.每个蛋白
有GFP, Flag等tag.Overexpression 做Co-IP.
先做A和B的相互作用，用protien A beads，IP后boil.先IP B, WB A.对照有（1）non-
transfected, （2）only transfected with A, 试验组（3）transfected with A and
B. IP后发现（1）没有A,（2）中有weak band of A,（3）中有strong band of A.
然后怀疑A蛋白太大，可能和beads或者antibody for IP有non-specific结合，所以（2
）中有weak band of A.之后change strategy: IP A, WB B.后来发现有同样情况。
请问下大家有没有发现蛋白和protein A beads non-specific结合的情况？
然后又换flag-crosslinked beads 做，IP B, WB A. 用Flag-pept... 阅读全帖 |
|
y**u
发帖数: 7459 | 3 他们用subtractive immunization作抗体，然后有一些candidate peptide(已经有了，
以前的积累，来源保密），研究对beta cell的作用，糖尿病方面的。计划是一年后交
前期结果，再要1－2m, 若有好的candiddate ab 或peptide 有作用，1-2年后5－10m.
只做到pre-clinical或phase1, 之后给大药厂。另外也会接一些赛选抗体的活儿自己挣
点钱。大老板作抗体出身的，资金的一半也是来自一个较大的抗体公司。 |
|
w******e
发帖数: 1187 | 4 http://www.nature.com/nbt/journal/v29/n6/full/nbt.1856.html
phage display是好东西。。。and又见next-gen seq in directed
evolution。。。well只做一个round的话不知还该不该叫evolution lol
不过这篇paper的pos control够单薄的。
Immune responses targeting self-proteins (autoantigens) can lead to a
variety
of autoimmune diseases. Identification of these antigens is important for
both diagnostic and therapeutic reasons. However, current approaches to
characterize autoantigens have, in most cases, met only with limited success
. Here we pres... 阅读全帖 |
|
b*****o
发帖数: 150 | 5 Sequence-specific DNA binding by covalently constrained peptide dimers of
the basic leucine zipper protein GCN4.
Bioorg Med Chem. 1995 Jun;3(6):777-84.
High Affinity, Sequence Specific DNA Binding by Synthetic Tripyrrole–Peptide Conjugates
b**********[email protected]
Thank you so much for your help! |
|
s****9
发帖数: 932 | 6 For an 50KD protein, there is no reason to keep GST. For some peptide
antigen (e.g. 50 peptides), you might consider keep GST in order to amply
the antibody responses.
For removing 20KD protein from 50KD proteins, the first portion I would
consider is 25KD-30KD dialysis.
和其他蛋白分开，进一步纯化？ |
|
j****x
发帖数: 1704 | 7 有没有人在做最针对这些个疑似receptor的药物，我也不是很清楚，只知道现在号称的
那些个receptor，没有一个是真正HBV specific甚至hepatocyte specific的，所以我
猜测这些不会是很好的target。PreS1 derived peptide作为entry inhibitor目前据说
效果已经非常好了，另有其他一些entry inhibitory peptide/molecule在pre-
clinical阶段的效果也不错，所以我估计眼下针对“疑似”receptor的rational
design不会有太大的吸引力。
chronic HBV治疗，要说最终解决还是得靠免疫学的方法，传统的NRTI也好，entry
inhibitor也罢，治标不治本，解决不了问题。强效Interferon以及前几天热炒的
Adoptive T cell therapy，我觉得应该是目前看来最有前景的方向吧，这玩意在国内
时市场太大了。
是了
用的 |
|
p*****m
发帖数: 7030 | 8 我理解你的意思 不过国内靠大分子药我觉得还是不靠谱 太贵了 用起来又不方便 1亿携
带者呢。。。国内还是要靠疫苗外加小分子药物。而小分子药物最直接的就是block病毒
entry.
当然了，你可以说乙肝携带者其实大多数的肝细胞都已经被侵染了 阻止entry用处不大
。但是现在乙肝在肝细胞之间怎么transfer的似乎了解的不是很清楚，也许阻止entry会
有意想不到的效果？（比如说，virus transfer对维持病毒的存在是关键的）
有没有人在做最针对这些个疑似receptor的药物，我也不是很清楚，只知道现在号称的
那些个receptor，没有一个是真正HBV specific甚至hepatocyte specific的，所以我
猜测这些不会是很好的target。PreS1 derived peptide作为entry inhibitor目前据说
效果已经非常好了，另有其他一些entry inhibitory peptide/molecule在pre-
clinical阶段的效果也不错，所以我估计眼下针对“疑似”receptor的rational
design不会有太大的吸引力... 阅读全帖 |
|
j****x
发帖数: 1704 | 9 有没有人在做最针对这些个疑似receptor的药物，我也不是很清楚，只知道现在号称的
那些个receptor，没有一个是真正HBV specific甚至hepatocyte specific的，所以我
猜测这些不会是很好的target。PreS1 derived peptide作为entry inhibitor目前据说
效果已经非常好了，另有其他一些entry inhibitory peptide/molecule在pre-
clinical阶段的效果也不错，所以我估计眼下针对“疑似”receptor的rational
design不会有太大的吸引力。
chronic HBV治疗，要说最终解决还是得靠免疫学的方法，传统的NRTI也好，entry
inhibitor也罢，治标不治本，解决不了问题。强效Interferon以及前几天热炒的
Adoptive T cell therapy，我觉得应该是目前看来最有前景的方向吧，这玩意在国内
时市场太大了。
是了
用的 |
|
p*****m
发帖数: 7030 | 10 我理解你的意思 不过国内靠大分子药我觉得还是不靠谱 太贵了 用起来又不方便 1亿携
带者呢。。。国内还是要靠疫苗外加小分子药物。而小分子药物最直接的就是block病毒
entry.
当然了，你可以说乙肝携带者其实大多数的肝细胞都已经被侵染了 阻止entry用处不大
。但是现在乙肝在肝细胞之间怎么transfer的似乎了解的不是很清楚，也许阻止entry会
有意想不到的效果？（比如说，virus transfer对维持病毒的存在是关键的）
有没有人在做最针对这些个疑似receptor的药物，我也不是很清楚，只知道现在号称的
那些个receptor，没有一个是真正HBV specific甚至hepatocyte specific的，所以我
猜测这些不会是很好的target。PreS1 derived peptide作为entry inhibitor目前据说
效果已经非常好了，另有其他一些entry inhibitory peptide/molecule在pre-
clinical阶段的效果也不错，所以我估计眼下针对“疑似”receptor的rational
design不会有太大的吸引力... 阅读全帖 |
|
K******S
发帖数: 10109 | 11 It depends on your protein's amino acid sequence and what kind of enzyme you
use. For example, trypsin cleaves c-terminus of K and R (not if the
following amino acid is P) so you could get peptides with various length.
Each mass spectrometer has its own mass range that will determine how big/
small it can detect. And if you use LC-MS/MS instrument, the analytical
column also contributes to the detection. C18 columns can't retain
hydrophilic peptides very well. |
|
K******S
发帖数: 10109 | 12 Just set up a search against human(or whatever species) database with no-
enzyme, the disadvantage is you can only find out "normal" peptides, if
there's a mutation or SNP, you can't get those information. Someone from
Vanderbilt just published a paper talking about their mutation/SNP database
searching.
peptide |
|
y*****x
发帖数: 14 | 13 MRM is mutiple reaction monitoring, is arguably most sensitive mass spec
analysis to monitor a known target through triple quadropole type mass
analizer
MS/MS usually means product ion scan, in protein/peptide mass spec, MS/MS
spectra were acquired to figure out the amino acid sequence in peptides,
that in most cases means to identify proteins in the sample |
|
r*********y
发帖数: 124 | 14 第一步IP用3XFLAG peptides洗脱之后，要不要先除去FLAG peptide，再做第二步IP？
谢谢 |
|
l**********1
发帖数: 5204 | 15 De rien.
plus
please go to
//www.ncbi.nlm.nih.gov/pubmed/22198840
for
The planarity of peptide bonds is an assumption that underlies decades of theoretical modeling of proteins
PNAS 2012 paper
but
Nonplanar peptide bonds in proteins are common and conserved but not biased toward active sites.
by Berkholz DS, Driggers CM, Shapovalov MV, Dunbrack RL Jr, Karplus PA.
Proc Natl Acad Sci U S A. 2012 Jan 10;109(2):449-
and above paper 1st author his PubMed total records:
//www.ncbi.nlm.nih.gov/pubmed?... 阅读全帖 |
|
g*********5
发帖数: 2533 | 16 thanks.
what the antibody name?
I want buy alas2.
the santa cruz and sigma one not work...
and Novus one's work in a paper, but its peptide include mitochondria
leading peptides... |
|
n******e
发帖数: 1246 | 17 Silica-Precipitating Peptides Isolated from a Combinatorial Phage Display
Peptide Library
Authors: Rajesh R. Naik1; Lawrence L. Brott1; Stephen J. Clarson2; Morley O.
Stone1
Source: Journal of Nanoscience and Nanotechnology, Volume 2, Number 1,
February 2002 , pp. 95-100(6)
发c*******[email protected]
谢谢啦 |
|
g*********5
发帖数: 2533 | 18 if I have a peptides sequence,
you help me synthesize the peptide and link it to KLH, inject rabbits and
10week service. how much you charge?
thanks |
|
J******9
发帖数: 736 | 19 谢谢。
另外老板还提到一个阻断这个抗原（比如A）的表达，是用A peptide来检测吗？该如何
处理？跟一般的IHC步骤一样吗？仅仅是将peptide替换一抗？
我完全没有概念，谢谢赐教~！ |
|
h********n
发帖数: 4079 | 20 blocking peptide, which can bind with the antibody. You can add the
peptide with the antibody to stain a positive sample. If the signal
disappears, it means the antibody has good specificity. My phd boss think
this is very important. |
|
F*K
发帖数: 608 | 21 用2步TAP然后打质谱找互作蛋白，除掉常见的contaminants，找到的大部分蛋白
peptide数都在1-2. 请问这个还有必要验证吗？一般经验peptide数要多大才可能是真
的互作蛋白？谢谢 |
|
R*****o
发帖数: 12 | 22 Our mAb libraries are made with 6 or more diverse peptide/soluble-domain
antigens, and recognize 4 - 12 epitopes on your target protein. These
libraries will multiply your chances of getting successful antibodies,
propelling your research to its ideal goal.
For only USD 1,499, you will receive a library
- consists of 6 monoclonal antibodies,
- made with 6 DIVERSE peptide antigens,
- within 4 months.
All you need to provide is your protein sequence.
Become one of our more than 1,200 customers wor... 阅读全帖 |
|
l**********1
发帖数: 5204 | 23 En
温习一下 其十一年前的豪言 啊
中国科学：显著的发展和严峻的挑战
——历史演变和现状比较
于2001年12月4日 1st version
饶毅
本文在简要回顾中国科学史的基础上，介绍一些近年研究的内容，肯定中国
科学令人乐观的进步，并讨论可能的意义。同时也指出，中国优秀论文总量仍不
到世界的百分之一，低于中国经济在世界所占的百分比、也不能适应中国持续发
展的要求。中国科学的规模需要相当程度的扩大、质量有待进一步提高。中国科
技还存在面临许多问题和挑战。
中国科学历史上的优秀例子
一个国家科学研究状况可以近似地由发表论文的情况所反映。以下，本文主
要从生命科学的研究来讨论中国科学的情况，一方面这是我有一定判断力的领域，
另一方面生命科学是科学技术最重要的组成部分之一，可以反映科学主流。讨论
中国论文发表情况前，先谈两个背景：中国科学的历史情况，优秀科学和著名杂
志的关系。
奠定中国生命科学研究是二、三十年代协和医学院生理系林可胜和生化系吴
宪。他们不仅自己研究出色，而且培养和带领了其他研究者。林可胜在胃肠道生
理和神经生理有优秀工作。1942年，他在中国当选为美国科学院外籍院士，是... 阅读全帖 |
|
l**********1
发帖数: 5204 | 24 sunny day
是专利的话 有基金可以负担没?
pls refer:
Spacer: As used herein, the term "spacer" (also referred to as "linker")
refers to a peptide sequence between two protein moieties in a fusion
protein. A spacer is generally designed to be flexible or to interpose a
structure, such as an alpha-helix, between the two protein moieties. A
spacer can be relatively short, such as the sequence Gly-Ala-Pro (SEQ ID NO:
4) or Gly-Gly-Gly-Gly-Gly-Pro (SEQ ID NO: 5), or can be longer, such as,
for example, 10-25 amino a... 阅读全帖 |
|
b******y
发帖数: 627 | 25 Not complicated for the last strategy. free thiol from cys reacts with
maleimide very efficiently. tri-maleimide is cheap. you have to synthesize
peptide anyway.
Just a reminder: the N-termini of proteins in vivo (bacteria or mammalian
cell) are complicated. The majority of them have the met cleaved upon
translation. Also the majority of them are N-terminally acetylated
cotranslationally or post-translationally.
Be careful! Just want to remind you not to use wrong peptides for Ab
generation. |
|
o****e
发帖数: 1011 | 26 多长的peptide？（多少amino acid residue？）
仅是peptide合成的话，所谓"N-Terminal Acetylation"不过是多了一小步简单的
合成而已，还不至于跟“敢不敢”什么的联系起来吧。
mammalian |
|
o****e
发帖数: 1011 | 27 乙酰化“很不麻烦”；
我们只买原料，一般自己合成peptide。还没买过外面合成的peptide。
所以没法推荐了。 |
|
R*Q
发帖数: 179 | 28 http://chou.med.harvard.edu/jameschou_CV.htm
Dr. James Chou
Education
1993 B.S. in Physics, University of Michigan, Ann Arbor
1994-1999 Ph.D. in Biophysics, Harvard University, Boston
Advisor: Professor Gerhard Wagner
46.
A formulation for correlating properties of peptides and its application to
predicting human immunodeficiency virus protease-cleavable sites in proteins.
Chou JJ.
Biopolymers. 1993 Sep;33(9):1405-14.
PMID: 8400033 [PubMed - indexed for MEDLINE]
Related citations
47.
The quin... 阅读全帖 |
|
R*Q
发帖数: 179 | 29 You know the trick if you compare his father's publications to his.
Chou KC's
Graphic analysis of codon usage strategy in 1490 human proteins.
Zhang CT, Chou KC.
J Protein Chem. 1993 Jun;12(3):329-35.
PMID: 8397791 [PubMed - indexed for MEDLINE]
A vector projection approach to predicting HIV protease cleavage sites in
proteins.
Chou KC, Zhang CT, Kézdy FJ.
Proteins. 1993 Jun;16(2):195-204.
PMID: 8332607 [PubMed - indexed for MEDLINE]
A new approach to predicting protein folding types.
Chou KC, Z... 阅读全帖 |
|
l**********1
发帖数: 5204 | 30 Bingo
suppl:
Boehm AM et al. (2007)
Precise protein quantification based on peptide quantification using
iTRAQ^@TM
BMC Bioinformatics. 8:214.
its Fig. 2
The normal-probability-plot shows that a lognormal distribution
fits the peptide ratio data. The transformed experimental
data is plotted and lies on a line, so the data is nearly
normally distributed. The x-axis denotes the inverse function
of the normality and the y-axis represents the sorted logtransformed
values.
//www.ncbi.nlm.nih.gov/pub... 阅读全帖 |
|
l*****n
发帖数: 24 | 31 马维骏
博士，研究员，博士生导师，教授
药物基因组学研究组组长
研究方向
从基因表达调控角度进行疾病的致病机理和药物防治研究，重点研究疾病发生
和药物作用下表达调控改变的早期应答基因。在生物体主要生理、病理、药理过程的早
期阶段，早期应答基因(ERG)的表达对其发展起关键作用。这些基因的快速表达具有即
早期急性动力学特征。不仅是转录调控，转录后水平调控同样对基因快速、瞬时表达起
重要作用，这是许多即早应答基因(IRG)的特征。大多数即早基因依赖其mRNA非编码区
的顺式作用元件实现转录后水平的调控。为了深入了解其复杂机制，有必要鉴别表达调
控异常的早期应答基因，系统分析验证各种顺式作用RNA调控元件、结合蛋白及其相互
作用。本实验室重点关注免疫应答及癌症发生相关早期应答基因的转录后调控在疾病发
生和药物防治过程中的影响和作用规律，探索它们在疾病诊治和药物研发方面的应用。
主要包括：
1. 免疫应答或癌症发生相关基因非编码区RNA顺式作用元件的鉴别与特性研究；
2. 即早基因在药物应答过程中的转录后水平调控；
3. 受信号转... 阅读全帖 |
|
s******9
发帖数: 283 | 32 "20到50之间的主流多肽要远贵于小分子，也比蛋白药物贵，因为蛋白药物可以用发酵
生产"
Any comment on engineering biological production platforms for longer
peptides? Some strategies such as fusing peptides to a protein carrier,
expressing them into inclusion body... |
|
|
A******y
发帖数: 2041 | 34 Sorry, my technical Chinese is not as good. You mean polypeptide?
Polypeptide is cheap once you industrialize the synthesis. even for 20-50 a
.a. you can just use Schultz technique and get grams of them (I know a lab
do that all the time). I'm suppose to be a classically trained peptide
chemist, but I am doing small molecule right now, Btw, for less than 20
residues, solution phase polypeptide could be even cheaper if you know how
to do it. My academic grandfather have a 3 million dollar N... 阅读全帖 |
|
K******S
发帖数: 10109 | 35 4倍的变化用LabelFree没有问题，我说的Subtle变化是指50%以下。
关于Ms定量的问题，不同的Peptide是不能比较的，因为序列不同会造成离子化的不同
，但是同样的Peptide在不同样品里是可以比较的。
现在Label Free有2个大的方向，1是Spectral Counting （MS2 Based），2是MS1
Based的那HPLC的峰面积来定量，我个人倾向于第2种，因为更准确。一般的HPLC定量用
Uv做Detector，LCMSMS只不过是用Ms做Detector。
当然这些都基于最近的仪器发展，现在Lc和Ms都是High Resolution了，所以一般的
Label Free没有问题。 |
|
a*******a
发帖数: 4233 | 36 peptide blocking撒。。
你chipseq下来总要找几个target做chip-realtime验证吧。
side by side用对应的peptide block掉抗体，再realtime检测target。
比较快，大家也认可。
组蛋白修饰的抗体非特异的很多，前阵子还专门有人发了个nature structure xxx验证
了几十种抗体，哪些特异哪些不特异。
An assessment of histone-modification antibody quality
http://t.cn/zWeHjmj
Antibody validation database
http://t.cn/zWeHjmW |
|
A****w
发帖数: 244 | 37 不是高手，不觉得有specifi interaction，很小的热量估计来自dilution。
到6.5 molar ratio还是有一点点热。。。
蛋白浓度是多少啊？peptide的浓度呢？
另外不知道你所谓的本底做了那些，有buffer to buffer, peptide to buffer, and
buffer to protein三组，你都比较过吗？
其他的就是你用的buffer是不是prefectly matched（同一batch buffer，蛋白透析好
。。。） |
|
C*********m
发帖数: 213 | 38 嗯，市面上能找到最小mwco的透析袋好像是1kDa, 估计我的peptide透析要损失60%以上
。这两个peptide表现不同可能是合成过程中的盐之类造成的。多谢。
dialysis |
|
G***G
发帖数: 16778 | 39 我有一个raw文件，这个文件有一个总图（图中xaxis是time，yaxis是relative
abundance）图中每一个x点对应一个scan。
每一个scan对应一个图（xasix是m/z，yaxis是relative abundance）
另外，经过蛋白质identification后，每个识别出的peptide还有一个图
（xasis是m/z, yaxis 是relative abundance）
有很多scan，很多识别出的peptide，
请问，这个reviewer的意思是让我添加什么高分率原图？ |
|
f******g
发帖数: 1003 | 40 http://www.merckmanuals.com/vet/pharmacology/anti-inflammatory_
&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&
Biochemical mediators released during inflammation intensify and propagate
the inflammatory response (see Anti-Inflammatory Agents: Actions of
Inflammatory MediatorsTables). These mediators are soluble, diffusible
molecules that can act locally and systemically. Mediators derived from
plasma include complement and complement-derived peptides and kinins.
Released via the classic or alte... 阅读全帖 |
|
s*********y
发帖数: 387 | 41 thanks. no DMSO is used in our system.
All in PBS or water buffer. the solubility of the peptide is very good.
Also, the Biotin-conjugated IgG has the same phenotype, but we can see an
obvious bell shaped curve, for the peptide it is a exponential growth curve,
making the interpretion intriguing... |
|
A******y
发帖数: 2041 | 42 Clearly you are not a peptide chemist. Synthetic peptide limits are around
50 amino acid residues if you are lucky. Expression system is a lot better. |
|
f******g
发帖数: 1003 | 43 A vaccine targeting mutant IDH1 induces antitumour immunity
Monoallelic point mutations of isocitrate dehydrogenase type 1 (IDH1) are an
early and defining event in the development of a subgroup of gliomas1, 2, 3
and other types of tumour4, 5, 6. They almost uniformly occur in the
critical arginine residue (Arg 132) in the catalytic pocket, resulting
in a neomorphic enzymatic function, production of the oncometabolite 2-
hydroxyglutarate (2-HG)7, 8, genomic hypermethylation9, 10, 11, genet... 阅读全帖 |
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f****n
发帖数: 723 | 44 Liu L., Li J., Tian X., Ren D. and Lin J. (2005) Information theory in
prediction of cleavage sites of signal peptides. Protein and Peptide Letters
, 12, 339-342
10年前的, 不知道谁能下载到?现在网上要价70, 不是盖的... |
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L****r
发帖数: 333 | 45 如果你想知道这些碎片是什么 （通常我们说的定性），你得用UPLC/HPLC-TOFMS或者
UPLC/HPLC-Orbitrap，如果有FTICR更好，这样你就能得到他们的isotopic mass （通
常我们称作精确分子量）, 注意，离子峰里面有很多是multiple-charge,你得去分析他
们， 为什么用柱子，前面的已经提到过，防止离子抑制，就是说，很多不同种类的中
性分子一道出来，在离子化的时候，有些离子被抑制掉了，这样你就得不到他们的峰，
用HPLC，他们就会一个一个地离子化，大大降低他们被抑制的可能。
然后定量，使用UPLC/HPLC-TRIPLEQUAD MS， 使用MRM，即用他们的碎片定量，一旦确
定是什么PEPTIDE，碎片就很容易得出，即使两个PEPTIDES有相同的分子量，不同结构
和相同的RETENTION TIME， 该仪器也能准确地定量他们。 |
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K******S
发帖数: 10109 | 46 ETD sensitivity/efficiency比CID低是肯定的
CID在ION TRAP里还是最快的
FUSION叫TRIBRID是你可以同时做3件事情.我觉得如何利用这个优势更重要.比如在
ORBITRAP做MS1或者HCD的时候别让ION TRAP闲着.
CID FRAGMENT的时候是M/Z FOCUS的,意味着如果PEPTIDE上的修饰容易掉的话,就不会有
很好的BACKBONE FRAGMENTS (因为修饰掉了M/Z就变了), ETD可以解决这个. 但现在有
HCD了,是QUADRUPOLE FRAGMENTATION (修饰掉了还可以继续FRAGMENTATION),对修饰的
PEPTIDE效果也不错.
尤其FUSION的速度太快了,15HZ ORBITRAP, 20HZ ION TRAP. 那这个当QE使唤有些大材
小用了. |
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a******g
发帖数: 71 | 47 peptide bond是amide bond，理论上是非常稳定的，只有强酸或者强碱催化下加热才能
水解，某些特定的序列，比如Asp-Pro这种，可以由于侧链之间的反应导致peptide
bond cleavage，但是即使是这样的反应在室温和偏中性pH下也是很缓慢的。如果SDS样
品煮完之后在一天之内有显著降解那要怀疑一下是不是有什么残存的蛋白酶活性。 |
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b******y
发帖数: 627 | 48 About UV crosslinking, many nucleic acid can be radicalized upon UV
treatment. Such radicals is highly reactive to active residuals in the
vicinity. Afterwards, digestion for MS. Abnormal peptides upon crosslinking
in comparison with those without crosslinking can tell you which peptide(s)
are close to the ligand. |
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a**y
发帖数: 464 | 49 Several postdoc positions are available in the group of Dr. Huiwang Ai at
the University of California, Riverside http://ailab.ucr.edu. The expertise of our lab is on protein engineering and fluorescent redox probes. Recently, our lab has explored new areas, including the biology and toxicology of oxidative stress, imaging of brain activities, and protein/peptide enzyme inhibitors.
We are looking for postdoctoral fellows with the following
expertise:
1. LC-MS/MS based proteomi... 阅读全帖 |
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l**********e
发帖数: 146 | 50 前一段时间把我的蛋白打了个mass spec，合作的人把测出的peptide序列用excel文档
发给了我。为了更好的分析各个区域的覆盖度，我想把这些peptide做在一个图里（像
下面链接中的那样），不知道用什么软件可以。多谢！ |
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