b*****g 发帖数: 2727 | 1 等效焦距应该是小孔到胶片平面的距离。不过我也没量过。用单反有点坑爹,取景器里
极暗,基本等于盲拍。 |
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m*********g 发帖数: 10735 | 6 为什么突然就成前辈了
我就大学快毕业的时候玩过一小阵子
说真的以前大学论坛上的那些都好会拍
很多都玩胶片,自己洗自己扫,还有自制pinhole的呢
随便搬一个出来都能秒杀整个买买提 |
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G****1 发帖数: 6908 | 7 Sharan SW-35 DIY Make and Shoot Panoramic Pinhole 35mm Film Camera Kit |
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G*********2 发帖数: 164 | 9 What's the question? Or what kind of question is this?
Any / all / every camera has DOF. Or more precisely, any / all / every lens
has DOF, since DOF is a characteristics of lens optics. And since any / all
/ every modern camera has lens, except pinhole camera, so what's the
question?
Oh, wait, the title question is: 哪个小的机器有 DOF? Answer: 有镜头的照相机
小机器有 DOF. |
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J**********i 发帖数: 925 | 10 Analogies and Metaphors Found in High School Essays
Her face was a perfect oval, like a circle that had its two other Sides
gently compressed by a Thigh Master. -
Sue Lin Chong, Washington
His thoughts tumbled in his head, making and breaking alliances like
underpants in a dryer without Cling
Free. -Chuck Smith, Woodbridge
He spoke with the wisdom that can only come from experience, like a Guy who
went blind because he
looked at a solar eclipse without one of those boxes with a pinhole in it
and |
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s****r 发帖数: 2386 | 11 I am going to put a pinhole camera on zongxin, so next time you visit him,
we will got it, lol |
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s**********9 发帖数: 1238 | 12 其实这些ROUTER都可以PORT FORWARDING,有些显示不一样而已,找找有没有PINHOLE的
TAB,有些牌子是在那里的。
LZ应该是要从OFFICE REMOTE ACCESS家里电脑而已。家里电脑用REMOTE DESTOP或者VNC
SERVER都可以。关键是在ROUTER上开必要的PORT。VNC你装的时候有提示啥端口。至于
自带的REMOTE DESTOP嘛,TCP 3389,如果内网有多于一台PC,另一台的默认端口还可
以改的。 |
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s******e 发帖数: 370 | 13 UV我曾经试过,不成功。
因为紫外光的能量太高,confocal扫又要小pinhole,时间很长,对sample损伤太大
我亲眼见到immersion oil被烧沸腾了 |
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s********n 发帖数: 342 | 14 最近在赶一个实验,做了CD4在脑部切片的IS,
信号很好,只是pattern很奇怪,大多数人show的staining是环状的,cytoplasm里表达
罗。
可是我的结果总是整个细胞都有信号,我用了两个不同公司的抗体,包括DAKO,用
confocal时
pinhole也设得很低,应该比核小多了。希望板上高手达人指教。谢谢。
另外,如果做cytokine和receptor的costaining,是不是假设所有cytokine都会bind到
receptor上呀?那这样的结果就是cytokine的staining包括了所有receptor的staining
呀。
恳请指教。 |
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h*******v 发帖数: 27 | 15 To my understanding, pinhole is just before PMT detector, and can't
exclude anything from the laser. The laser beam will penetrate the whole
sample...Hope someone can clarify that. |
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s*****j 发帖数: 6435 | 16 就一个pinhole. 激光直接fiber couple进入scanhead.
这个不能看airy disk的图, 得看公司提供的scan-head的光路图. |
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Z****9 发帖数: 738 | 17 pinhole是PMT前面的一个小孔,用来屏蔽样本上out focus的光,与laser对样本的
bleach没有任何关系
laser在不同的位置的强度可以通过公式计算出来,bleach的速度与laser的强度相关,
理论上是可以计算的
但是由于不同的样本对光的穿透性不同,以及样本对光的散射,导致无法精确计算在样
本上不同位置的光强度
如果你的东西bleach特别快,用3d扫描不是一个很可行的方法
当你在扫描某一个layer的过程中,你同时bleach所有layer的样本 |
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a*****x 发帖数: 901 | 18 第一个问题,可以用ZEN或者LSM读原始数据加Scale bar。如果用其他软件,根据像素
数目和显微镜参数也可以做。
第二个问题,怀疑光路设置有问题。比如选反射镜的时候可以控制反射的波长范围的,
可以降低在其他channel的信号。和pinhole及gain的设置也有关系。 |
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Z****9 发帖数: 738 | 19 confocal用的是PMT detector (大部分),这个的对光的检测效率(quantum
efficiency)在30-40%左右,对red 或者far red的检测效率更低
普通的fluorescent microscopy 用的是CCD 或者EMCCCD,效率在60-70%
confocal由于pinhole的存在,不但屏蔽了out focus的光子,同时也屏蔽了很多有效的
光子。
这两个原因,造成了confocal比普通的fluoresent microscopy的bleaching严重
a
sample。
slide |
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E****a 发帖数: 118 | 20 如果大牛们的审稿够的话,请转让审稿机会给我吧(审稿邮箱:fawa5960@jila.
colorado.edu), 影响因子不限,国内外杂志都可以。
本人第二年博后了(一直在美国),我的方向是分子光谱(红外可见光)研究,但
审稿物理化学方向都可以,读博士之前也有一些催化方向的经验,以下是自己的简单介
绍:
I received my Bachelor’s of Science degree in Chemical Physics from
University of Science and Technology of China in 2008. During this I had two
year research experience on surface science and catalysis of model
catalytic systems. my undergraduate thesis was “Synthesis and Catalysis
study of N-doped TiO2 in nano-scale”. I completed my PhD de... 阅读全帖 |
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w*******y 发帖数: 60932 | 22 Features14 Megapixel: An amazing 14 million pixels in the high-resolution
CCD create superior image quality. Create poster-sized prints without losing
a single detail.
7x Wide-angle Zoom: Get more than twice the magnification of a 3x zoom
with this ultra-slim 7x optical zoom.
3" LCD: This huge 3" LCD features an extra-wide viewing angle and anti-
glare technology for easier composing and shooting even in direct, bright
sunlight
One-Touch HD Movie: With One-Touch HD Movie you can reco... 阅读全帖 |
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w*******y 发帖数: 60932 | 23 This 9v Car Charger comes with multiple adapters to fit your sized plug.
Most of the sellers indicate that you either need the Yellow tip charger or
the Black tip charger.......
This Car Charger:
http://www.mcbub.com/item/Car-Charger-For-Zenithink--ZT-180-Ser
gives you all options, plus the pinhole charger for the 2nd Gen ZT-180
tablets.....all for $4.99 shipped.
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c******s 发帖数: 5583 | 24
...他真是烧了一圈了. alpa似乎不大符合他一贯作风.估计要烧也是烧自制pinhole之
类吧... |
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