l**********1
发帖数: 5204 | 1 pls try Multiplex PCR by A, B and C three primers set (below figure that
X, Y, Z as A, B, C primers)
PMID 19936220
Generation and characterization of mice carrying a conditional allele of the
Wwox tumor suppressor gene. (2009).
PLoS One. 4: e7775.
http://www.ncbi.nlm.nih.gov/pubmed/19936220
or refer
Biology版 - 有没有可能出现假KNOCKOUT的情况?
hashuoy
2010-09-09 13:52:31
来自: 192.168.
1从公司订制的KO老鼠,带CRE基因,大半年折腾下来去掉CRE并拿到大量杂合子,按照公司
给的DATA SHEET, GENOTYPING双引物鉴定老鼠各基因表型正常,符合孟德尔遗传,但在对
培养的野生型和纯合子MEF细胞鉴定时,我发现虽... 阅读全帖 |
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b******k
发帖数: 2321 | 2 Dopamine-mushroom body circuit regulates saliency-based decision-making in
Drosophila.
Zhang K, Guo JZ, Peng Y, Xi W, Guo A.
Science. 2007 Jun 29;316(5833):1901-4.
PMID: 17600217 [PubMed - indexed for MEDLINE]
Crossmodal interactions between olfactory and visual learning in Drosophila.
Guo J, Guo A.
Science. 2005 Jul 8;309(5732):307-10.
Choice behavior of Drosophila facing contradictory visual cues.
Tang S, Guo A.
Science. 2001 Nov 16;294(5546):1543-7.
三篇，挺不错了。。国内除了做结构的有几个发过三篇CNS的？ |
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l**********1
发帖数: 5204 | 3 E.Coli. or normal Cytoplasmic cells 可以试试降低表达温度 for expanding
target protein folding time
but Archaea Cells or Mitochondria cells might need to increase temperature
that can contribute to expand folding time.
Just think about before 2.6 Billion Ys ago low oxygen and without Ozone
layer the Archaea or Cyanobacteria living where (almost ocean or yellow
stone spring bottom) and there average temperature is how many degree?
more pls refer PMID 22754756
CRISPR/Cas systems in archaea: What array space... 阅读全帖 |
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s****9
发帖数: 932 | 4 我刚才吃饱了撑着pubmed了一下“NTCP and HepG2”，HepG2确实不表达NTCP。为了用
HepG2研究NTCP，都需要做stable transfection。
看这段：
“Human hepatoblastoma cells (HepG2), cells that have lost native Ntcp
expression, were stably transfected with the rat Ntcp gene.”
PMID: 14598021。
不知道你们实验室是不是做HBV的，还是刚刚半路出家。
HBV |
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l**********1
发帖数: 5204 | 5 1964 Tokyo Summer Olympic then 2012 Yamanaka S,
before then 1987 Tonegawa S also got nonel but TS did his noble paper at
Basel Institute for Immunology in Basel, Switzerland, so it was not
indepedent Japan's contribution
2008 Beijing Summer Olympic (original should be 2000Y) so 2048 or about
at least 10 Ys earlier around 2038 100% contributed CHINA's noble (biomedi)
that dream might come true.
ps: before than even from Taiwan Nobel (biomedi) might be more earlier than
mainland China... 阅读全帖 |
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l**********1
发帖数: 5204 | 8 莫不是用了张江同步上海光源的帝都 lab PI 又有了cns的last/corresponding
authorship 都能挂名南边的魔都的
中科院 评院士 阿
cited:
这项成果是由中国科学院与中国疾病预防控制中心合作完成，上海同步辐射设施提供了
重要的技术支撑。
Science：高福课题组破解H7N9禽流感病毒感染人奥秘
作者： 来源：北京生命科学研究院 发布者：亦云 日期：2013-09-06 今日/
总浏览：
继不久前在《柳叶刀》和《科学》杂志发表禽流感重要论文之后，中国科学院北京
生命科学研究院/微生物研究所高福 (George F Gao) 课题组在H7N9禽流感病毒感染人
的跨宿主传播机制
研究上又有新的突破，9月5日，《科学》杂志在线发表该课题组题为“人感染H7N9禽流
感病毒的受体结合特性与结构基础”的文章。
http://www.biotech.org.cn/information/111652
PMID 24009358
Shi Y, Zhang W, Wang F et al. Gao GF. (2013).
Structures... 阅读全帖 |
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c********n
发帖数: 67 | 9 Lipid oxidation and peroxidation in CNS health and disease: from molecular
mechanisms to therapeutic opportunities
PMID: 19624272
Thanks a lot for your kindly help.
h***[email protected] |
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w***a
发帖数: 432 | 10 这片文章里有讲过一点。和shRNA一样表达。
@Article{Chen2004,
Title = {MicroRNAs modulate hematopoietic lineage
differentiation.},
Author = {Chang-Zheng Chen and Ling Li and Harvey F
Lodish and David P Bartel},
Journal = {Science},
Year = {2004},
Month = {Jan},
Note = {mark},
Number = {5654},
Pages = {83--86},
Volume = {303},
Abst... 阅读全帖 |
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p******2
发帖数: 47 | 11 Conditional gene targeting in macrophages and granulocytes using LysMcre
mice.
Clausen BE, Burkhardt C, Reith W, Renkawitz R, F爀猀琀攀爀 I.
Source
Institute for Genetics, University of Cologne, Germany
PMID: 10621974
请发到i************[email protected]
新人没有包子，先行跪谢了！ |
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m**********3
发帖数: 706 | 15 今天查文献，关键词搜索" survival，retinal neurons". 有一篇文章让我很迷惑。仔
细一看名字和地址，莫非是气功大师的杰作。求鉴定。如果真是，那严大师应该是所有
这些大师，教主里头科研素质最高的了。放在国内都可以PHD毕业了。甚至秒杀大部分
博导.
Int J Neurosci. 2002 Oct;112(10):1209-27.
XY99-5038 promotes long-term survival of cultured retinal neurons.
Yan X, Fong YT, Wolf D, Shen H, Zaharia M, Wang J, Wolf G, Li F, Lee GD, Cao
W.
Source
Institute of Chongqing Traditional Chinese Medicine, Chongqing, Sichuang, P.
R. of China.
Abstract
We have previously reported that XY99-5038, a preparation from a spec... 阅读全帖 |
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d****d
发帖数: 214 | 16 可以试一下PMID: 21970951吗？谢谢！ |
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H*Q
发帖数: 725 | 17 email, h********[email protected]
thanks a million
Nat Med. 2011 Nov 7;17(11):1359-70. doi: 10.1038/nm.2537.
Tumor angiogenesis: molecular pathways and therapeutic targets.
Weis SM, Cheresh DA.
Source
Department of Pathology and Moores University of California-San Diego Cancer
Center, University of California-San Diego, La Jolla, California, USA.
Abstract
As angiogenesis is essential for tumor growth and metastasis, controlling
tumor-associated angiogenesis is a promising tactic in limiting cancer
pro... 阅读全帖 |
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w******h
发帖数: 47 | 20 Extracellular matrix and growth factors in salivary gland development.
Sequeira SJ, Larsen M, DeVine T.
Front Oral Biol. 2010;14:48-77. doi: 10.1159/000313707.
PMID: 20428011
请发至w*******[email protected]
不甚感激！ |
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c********n
发帖数: 67 | 21 Inside-Out Red Cell Membrane Vesicles: Preparation and Puirification
PMID: 5418644
Thanks very much for you kindly help.
h***[email protected] |
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m*n
发帖数: 695 | 22 学校没有全文，如果哪位能帮助，一枚包子致谢!
a******[email protected]
Curr Alzheimer Res. 2013 Aug 6. [Epub ahead of print]
Resilient Brain Aging: Characterization of Discordance between Alzheimer's
Disease Pathology and Cognition.
PMID: 23919768 |
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m***o
发帖数: 272 | 23 Curr Diabetes Rev. 2012 Jul 1;8(4):285-93.
FGF21: The center of a transcriptional nexus in metabolic regulation.
Adams AC, Kharitonenkov A.
PMID: 22587513
谢谢! |
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a****d
发帖数: 1919 | 24 J Theor Biol. 2009 Jan 21;256(2):164-79. doi: 10.1016/j.jtbi.2008.09.034.
Epub 2008 Oct 15.
Cell lineage tree models of neurogenesis.
PMID: 18977364
please send to g********[email protected], thanks! |
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l**********1
发帖数: 5204 | 25 if relevent to quantivie analysis of TF binding sites and chromatin
accessibility,
first microarray then NGS might be a sutiable choice.
pls refer
PMID 23739687
Shu H, et al., (2013).
Measuring Arabidopsis chromatin accessibility using DNase I-polymerase chain
reaction and DNase I-chip assays.
Plant Physiol. 162: 1794-801.
Abstract
DNA accessibility is an important layer of regulation of DNA-dependent
processes. Methods that measure DNA accessibility at local and genome-wide
scales have faci... 阅读全帖 |
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a****d
发帖数: 1919 | 26 Cold Spring Harb Perspect Biol. 2012 Oct 1;4(10). pii: a008359. doi: 10.1101
/cshperspect.a008359.
Molecular control of neurogenesis: a view from the mammalian cerebral cortex.
PMID: 23028117
Please send the PDF file to g********[email protected], thanks! |
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l**********1
发帖数: 5204 | 27 非植物 或哺乳的数据库区别也 植物的也 一样在完善
关键是楼主有无NGS and HMM （Hidden Markov Models 的背景或能找到那种背景的
合作人
pls refer
PMID 23435661
by Van der Does D et al., (2013).
Salicylic acid suppresses jasmonic acid signaling downstream of SCFCOI1-JAZ
by targeting GCC promoter motifs via transcription factor ORA59.
Plant Cell. 25: 744-61.
Abstract:
ignored
In silico promoter analysis of the SA/JA crosstalk transcriptome revealed
that the 1-kb promoter regions of JA-responsive genes that are suppressed by
SA are significantl... 阅读全帖 |
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l**********1
发帖数: 5204 | 28 RE LZ
Did you note that some organism, anti-sense RNA likes RNAi can assist target
protein trans-splicing.
if not , pls go to one
2011 PhD dissertation of Université de Montréal
by Yan YF
pdf web link:
HTTPS double dot //papyrus.bib.umontreal.ca/xmlui/bitstream/handle/1866/5423
/Yan_Yifei_2011_memoire.pdf?sequence=5
its pp24:
>Does anti-sense mRNA assist trans-splicing?
A mechanism was recently identified in the ciliate Oxytricha trifallax
where larger RNA molecules are used as templates to dir... 阅读全帖 |
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l**********1
发帖数: 5204 | 29 pRS416 excepts T7 also has Plac promoter.
pls refer
PMID 16820502
http://www.ncbi.nlm.nih.gov/pubmed/16820502
>pRS416 is a centromeric (CEN) plasmid and therefore is maintained at 1 to 2
copies per cell, while pRS426 is a 2μm plasmid, maintained at about 25
copies per cell (36). Both plasmids have the ColE1-derived pBluescript high-
copy origin of replication, a lacZα-associated multicloning site, an
ampicillin resistance gene (bla) for gram-negative bacteria, and a URA3
marker for yeast. These... 阅读全帖 |
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l**********1
发帖数: 5204 | 32 >发信人: sunnyday (胖头鱼。按斤卖就赚了), 信区: Biology
标 题: Re: sunnyday，你再讲讲BT转基因粮食的看法吧
发信站: BBS 未名空间站 (Sun Oct 20 19:48:54 2013, 美东)
ignored stuff
如果中国的转Bt蛋白水稻被批
准的话，将是世界上第一个被广泛食用转Bt蛋白的作物
-----
哪国人都能用Bt 水稻或小麦, 但以三聚氰氨为代表的 '一切向钱看, 摸着石头过河'
的土鳖用了的话
有再来一次 <<寂静的春天>> 的 可能 范围是横跨整个欧亚大陆
http://zh.wikipedia.org/wiki/寂静的春天
n.b.
Cry1F Bt 不是对脊椎动物的 而是对非对象的有益无脊椎动物 比如蜜蜂等
土鳖可以做到致死率 不是以下论文的No. 4 的<1% 而是 No.3 >50% 吧
pls refer
3. The greatest source of variability in estimated mortality was species
sensitivity. Before... 阅读全帖 |
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h**********n
发帖数: 617 | 34 需要一片paper讲journal club,是science的。
PMID: 24051248
Science. 2013 Sep 19. [Epub ahead of print]
Circadian Clock NAD+ Cycle Drives Mitochondrial Oxidative Metabolism in Mice.
谢谢！
我的邮箱：
h*******[email protected] |
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l**********1
发帖数: 5204 | 35 >有可能在不同的条件下可以变换被ubiquitination的位点(从一个lysine换到另
一个lysine)。
Yes, pls check PMID 22588506,
HTTP double dot//www.weizmann.ac.il/sb/faculty_pages/Levy/sites/weizmann.ac.
il.sb.faculty_pages.Levy/files/c2mb25052g.pdf
or
Hagai T et al., (2012).
The origins and evolution of ubiquitination sites.
Mol Biosyst. 8: 1865-77.
http://www.ncbi.nlm.nih.gov/pubmed/22588506
its Fig. 3 attached here. |
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l**********1
发帖数: 5204 | 37 So,
better is to perform multiple GSA running,
pls refer attached figure
cited from
PMID 23444143
Väremo L et al., (2013).
Enriching the gene set analysis of genome-wide data by incorporating
directionality of gene expression and combining statistical hypotheses and
methods.
Nucleic Acids Res. 41: 4378-91.
>http://www.ncbi.nlm.nih.gov/pubmed/23444143 |
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l**********1
发帖数: 5204 | 38 >只有千万分之三?
are they sure?
即使覆盖了 除了港澳台的 CN 所有地域的 地下 和地上的基因测序
也不算完整
n.b. 秦末的 徐福东渡日本的 说法 还有三国归晋之前后 曹操家族 也可能东渡或东
跨 韩日
更不要说外逃南洋的客家 很大一部分是黄河以北 魏国之后裔呢
即使后面唐宋 的音声更接近客语而不是元和清之后的后清 普通话:
pls refer,
>唐宋漢語之入聲調(收-p,-t,-k塞音尾)，今之普通話已完全消失，客語卻完整保留，
如：「合喝核」「接節跡」「業熱>逆」…又如雙唇鼻音尾(閉口ｍ尾)的字，北京音系
消失，併入舌尖鼻音尾(ｎ尾)，如「新心」「因音」「干甘」「山>衫」「鄰林」「煎
尖」「攤貪貪」「真針」等原來韻尾有別的字(ｍ/ｎ不同)，北京話卻都混同了，這些
收ｍ尾的字不僅保留在客語中，也留存於韓語中，可說是「禮失而求諸野」。客語的鼻
韻尾-m,-n,-ng和入聲尾-p,-t,-k對應整齊(如:斬展整、敢揀哽；眨舌石、磕刻揢)，韓
語也一樣(韓語的輔韻尾「終聲」有-m,-n,-ng，-p,-t,-k,-l七個)，因此學會客語，... 阅读全帖 |
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m**********n
发帖数: 299 | 40 http://www.ncbi.nlm.nih.gov/pubmed/?term=24014010
1: Garvey WT. New tools for weight-loss therapy enable a more robust medical
model for obesity treatment: rationale for a complications-centric approach.
Endocr Pract. 2013 Sep-Oct;19(5):864-74. doi: 10.4158/EP13263.RA. PubMed
PMID:
24014010.
非常感谢。 |
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l**********1
发帖数: 5204 | 41 Plus PMID 23953117 Lim WA@UCSF new Cell paper:
Coyle SM, et al., (2013).
Exploitation of latent allostery enables the evolution of new modes of MAP
kinase regulation.
Cell. 154: 875-87.
n.b. MAPK 变构反应 原来是为了进化潜规则 中 起到调控另外的pathway/s 开关作用
的...
>Abstract
ignored stuff
>The magnitude of this latent allosteric potential drifts widely among pre-
Ste5 MAP kinases, providing a
pool of hidden phenotypic diversity that, when revealed by new activators,
could lead to functional divergence and to the evo... 阅读全帖 |
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l**********1
发帖数: 5204 | 42 Before NGS 2.0 or 3.0 Illumina platform can read enough longer different
isoforms within Exome sequencing and systems biology aspect,
it is still elusive to answer the linkage between SNP2.0 or 3.0 alternative
splicing with
syndromic disease...
Pls check,
>Although previously described in TS, no CACNA1C mutations have been
reported for
>non-syndromic LQTS. With our functional studies confirming Cav1.2 gain-of-
function as the cellular basis for the CACNA1C mutation-positive patient’s
LQTS pheno... 阅读全帖 |
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l**********1
发帖数: 5204 | 43 Plus PMID 23953117 Lim WA@UCSF new Cell paper:
Coyle SM, et al., (2013).
Exploitation of latent allostery enables the evolution of new modes of MAP
kinase regulation.
Cell. 154: 875-87.
n.b. MAPK 变构反应 原来是为了进化潜规则 中 起到调控另外的pathway/s 开关作用
的...
>Abstract
ignored stuff
>The magnitude of this latent allosteric potential drifts widely among pre-
Ste5 MAP kinases, providing a
pool of hidden phenotypic diversity that, when revealed by new activators,
could lead to functional divergence and to the evo... 阅读全帖 |
|
l**********1
发帖数: 5204 | 44 Before NGS 2.0 or 3.0 Illumina platform can read enough longer different
isoforms within Exome sequencing and systems biology aspect,
it is still elusive to answer the linkage between SNP2.0 or 3.0 alternative
splicing with
syndromic disease...
Pls check,
>Although previously described in TS, no CACNA1C mutations have been
reported for
>non-syndromic LQTS. With our functional studies confirming Cav1.2 gain-of-
function as the cellular basis for the CACNA1C mutation-positive patient’s
LQTS pheno... 阅读全帖 |
|
m**********n
发帖数: 299 | 45 包子求助文献一篇
http://www.ncbi.nlm.nih.gov/pubmed/?term=24014010
1: Garvey WT. New tools for weight-loss therapy enable a more robust medical
model for obesity treatment: rationale for a complications-centric approach.
Endocr Pract. 2013 Sep-Oct;19(5):864-74. doi: 10.4158/EP13263.RA. PubMed
PMID:
24014010.
非常感谢。 |
|
|
m**********n
发帖数: 299 | 47 包子求助文献一篇
http://www.ncbi.nlm.nih.gov/pubmed/?term=24014010
1: Garvey WT. New tools for weight-loss therapy enable a more robust medical
model for obesity treatment: rationale for a complications-centric approach.
Endocr Pract. 2013 Sep-Oct;19(5):864-74. doi: 10.4158/EP13263.RA. PubMed
PMID:
24014010.
非常感谢。 |
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