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全部话题 - 话题: polymerize
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s******y
发帖数: 28562
1
来自主题: Biology版 - Making blood vessels on 3D printers
http://www.digitaljournal.com/article/311591
Though 3D printing rapidly produces 3-dimensional objects, including
microstructures, from diverse materials by applying layers and bonding them
with UV radiation, this advanced technology is still too imprecise to
generate ultra-fine, complex capillary vessels, so the researchers developed
a two-photon polymerization process, where intense, brief, highly focused
laser pulses strike the material, stimulating a crosslinking between
molecules, and the m... 阅读全帖
h*****x
发帖数: 147
2
来自主题: Biology版 - paper help!
Anal Bioanal Chem. 2011 Dec 27.
Highly sensitive detection of microRNA by chemiluminescence based on
enzymatic polymerization.
Ma C, Yeung ES, Qi S, Han R.
http://www.springerlink.com/content/tk85jw36313231l4/
please send to: h************[email protected]
Thanks a lot!
l**********1
发帖数: 5204
3
GTP→GDP already
please go to new book
"New Insights into the Mechanisms
of Cytomotive Actin and Tubulin
Filaments" 2011 Elsevier Inc.
by Christopher H.S. Aylett, Jan Lo¨we, and Linda A. Amos
full text link:
//www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/AmosReview2011.pdf
its pp20
On phosphate release, Wang and Nogales proposed that the contact
between T7 and the nucleotide of the adjacent subunit within the E-site
interface becomes disrupted, leaving contacts only on the side of the
subunit–subunit ... 阅读全帖
l**********1
发帖数: 5204
4
Continue:
please look a lo below figure
cited from upper floor that E-Book.
s****n
发帖数: 234
5
Hope these will help:
http://mitchison.med.harvard.edu/protocols/poly.html
http://hymanlab.mpi-cbg.de/hyman_lab/methods/tubulin/tubulin_pr
http://www.mpi-cbg.de/nc/research/research-groups/joe-howard/pu (look for "Microtubule dynamics reconstituted in vitro and imaged by single-molecule fluorescence microscopy")
BTW: what TIPS proteins?

by
l**********1
发帖数: 5204
6
Are you sure you can use only fluorescence microscopy来研究tubulin dynamics
in vitro regulated by other tip protein?
http://www.mitbbs.com/article_t1/Biology/31629913_0_2.html
27th floor
If not,
please just go to
//cryoem.berkeley.edu/microtubules.html
//cryoem.berkeley.edu/cryoem
//cryoem.berkeley.edu/microscopes
more just click all icons you can open that web sites.
//mcb.berkeley.edu/index.php?option=com_mcbfaculty&name=nogalese
then rethink about which kind of instrument will be needed?

by
s****n
发帖数: 234
7
I think the in vitro tubulin dynamics experiments using TIRF microscopy will
be able to say whether the presence of tip protein(s) affects the tubulin
rescue and shrink rates. A complete understanding of the underlying
molecular mechanism may require structural studies using other methods
including EM.

dynamics
l**********1
发帖数: 5204
8
Exactly your opinion is right.
plus even without CryoEM below group still can do simulation with modeling for their most possbile
mechanism discovery
please refer the figure G its scale bar 200 nm it is below florescent microscopy resolution limits,
so they cannot resolve the fusion of the individual FIP3-endosomes.
cited from
//www.ncbi.nlm.nih.gov/pubmed/21486954
and
//www.ncbi.nlm.nih.gov/pubmed/16818889

will
d******1
发帖数: 709
9
来自主题: Biology版 - 蛋白反复解冻会造成什么问题
A more recent, biophysical definition is broader, including any polypeptide
which polymerizes to form a cross-beta structure, in vivo, or in vitro. Some
of these, although demonstrably cross-beta sheet, do not show some classic
histopathological characteristics such as the Congo red birefringence.
http://en.wikipedia.org/wiki/Amyloid
t*********o
发帖数: 78
10
来自主题: Biology版 - Dr. Suo招破死刀,有wsn敢上吗?
Postdoctoral Position in Single Molecule Enzymology : Columbus, OH, United
States
One postdoctoral position is available immediately in an enzymology
laboratory to elucidate the kinetics and dynamics of polymerization
catalyzed by human and viral DNA/RNA polymerases using single molecule Prism
-based or Objective-based Total Internal Reflection Fluorescence (TIRF)
apparatus. Background in single molecule technologies, computer programming,
enzyme kinetics, biophysics, and protein purification is... 阅读全帖
s*******2
发帖数: 598
11
See below 1997 paper
Biochem J. 1997 Apr 1;323 ( Pt 1):233-7.
Actin is cleaved during constitutive apoptosis.
Brown SB, Bailey K, Savill J.
SourceDivision of Renal and Inflammatory Disease, Department of Medicine,
University Hospital, Nottingham NG7 2UH, UK.
Abstract
Proteases play an important role in the programme of cell death by apoptosis
but little is known of the substrates cleaved, particularly in constitutive
models of this type of cell death. Neutrophils spontaneously undergo
apoptosis ... 阅读全帖
x*******6
发帖数: 994
12
来自主题: Biology版 - 找文章reviewer
现手头有一篇文章,找reviewer,我不是此领域。关键词:In vitro cell culture,
platelet adhesion tests ,in vivo implant tests,plasma-polymerized para-
xylene films
请发简历回站内邮箱。
v***a
发帖数: 1242
13
最近一直做siRNA transfection做不出,找不出问题在哪。想换一个转染试剂。
没有用过主流的Lipofectamine系列,不知道哪一款比较适合primary mammalian Cells?
还看到Mirus Bio 的 TransIT-X2 广告,说“TransIT-X2 is an innovative non-
liposomal, polymeric system that has better transfection performance than
Lipofectamine 2000”,不知是真是假?
恳请高手推荐个好用的。
l****5
发帖数: 2968
14
来自主题: Biology版 - paper helps!
请发g***********[email protected]
包子酬谢,多谢大家!
1. Effective natural dye extraction from different plant materials using
ultrasound
2. Anthocyanins: natural colorants with health-promoting properties.
Radish Anthocyanin Extract as a Natural Red Colorant for Maraschino Cherries
3. Separation and recovery of food coloring dyes using aqueous biphasic
extraction chromatographic resins
4. Recovery of anthocyanins from grape pomace extracts (Vitis vinifera L. cv
. Cabernet Mitos) using a polymeric adsorber resin... 阅读全帖
O********4
发帖数: 113
15
来自主题: Biology版 - 能拿炸药奖吗?
没错,我在考虑这事情过去以后可以去趟一下这个浑水。类似的铁硫蛋白都可能有意想
不到的活力。
我和这哥们在波士顿的时候算牌友,他以前做integrin的,但我离开波士顿后很多年没
联系了。知道他回国并做得并不顺,几年没什么paper, 没想到憋大招呢! 不过被黑
的太厉害了,世道险恶啊。不过他应该能得到该得的credit.
既然是生物版,我们猜下这个protein 的机理吧,这么小的蛋白,我猜一定是
magnetic filed induces protein polymerization, assembling into a larger
complex that eventually depolarizes membrane. Other components may also be
recruited, so you should look at their interacting proteins.
O********4
发帖数: 113
16
来自主题: Biology版 - 能拿炸药奖吗?
没错,我在考虑这事情过去以后可以去趟一下这个浑水。类似的铁硫蛋白都可能有意想
不到的活力。
我和这哥们在波士顿的时候算牌友,他以前做integrin的,但我离开波士顿后很多年没
联系了。知道他回国并做得并不顺,几年没什么paper, 没想到憋大招呢! 不过被黑
的太厉害了,世道险恶啊。不过他应该能得到该得的credit.
既然是生物版,我们猜下这个protein 的机理吧,这么小的蛋白,我猜一定是
magnetic filed induces protein polymerization, assembling into a larger
complex that eventually depolarizes membrane. Other components may also be
recruited, so you should look at their interacting proteins.
c******g
发帖数: 49
17
来自主题: Biology版 - endocytosis 调控因子
Actin polymerization 不会影响1,2

:1. clathrin knockdown ---> clathrin dependent endocytosis
:2. caveolin knockdown ---> Caveolae-dependent Endocytosis
S*****n
发帖数: 6055
18
steam reforming to syngas
then to methanol
then MTP to propylene
then propylene polymerization
z****y
发帖数: 613
19
来自主题: ChemEng版 - Question: process for polymerization
z****y
发帖数: 613
20
来自主题: ChemEng版 - Question: process for polymerization
I think, for the irregular particles --- use mechanical grinding;
for the regular particles --- use suspension or emulsion
polymerisaion; dispersion or precipitation polymerisation.
f*******r
发帖数: 78
21
来自主题: ChemEng版 - 选择方向求助
本人生物本科,有一些polymeric drug delivery和nano-medicine方面的研究经历,目前
正考虑申请PH.D
目前感觉化工方向下的一些生物化工(drug delivery, biofuel), BME,还有
pharmaceutical science都适合生物专业申请. 不知各位对与生物结合的新型化工怎么
看? 如果从将来就业前景(而非研究前景)来看,以上哪个方向更适合我呢?
希望大家多多指教
c*********3
发帖数: 26
22
来自主题: ChemEng版 - 复合材料方面的博士后职位
替一国内的朋友,问问版上的朋友有没有 关于复合材料方面的博士后的职位招人的。
研究方向是:(我一点也不懂啦)
Nanostructured materials and their characterization
Polymeric Composite Self-repairing
Nanotructural & Organic Intelligent Materials
谢谢了。请回站内信箱。
G***6
发帖数: 1323
23
# Carbon nanotubes
# Macromolecules at the surface and interface
# Nanofiber/nanoparticle filled polymers
# Nanocomposites
# Optoelectronic polymers
# Polymeric biomaterials
# Polymer photonic and biomedical devices (sensors, actuators, light-
emitting diodes)
在以上这些领域做器件的 目前的工作前景如何呢? 这个领域的硕士和博士找工作有
什么区别啊? 请赐教!不吝感谢!
l***o
发帖数: 16
24
浙江大学化学工程与生物工程学系是由化工教育先驱李寿恒教授于1927年在中国创建的
第一个化工系,是化工领域高水平人才的重要培养基地。现有化学工程与工艺、生物工
程、制药工程、过程装备与控制工程4个本科专业,拥有化学工程与技术学科博士点和
博士后流动站,涵盖化学工程、化学工艺、生物化工、应用化学、工业催化、制药工程
、化工过程信息工程等专业,同时建有动力工程学科下的化工过程机械、动力装备信息
工程博士点,并共建动力工程及工程热物理博士后流动站。其中,化学工程、化工过程
机械为国家重点学科,生物化工为国家重点(培育)学科,生物化工和应用化学为浙江
省重点学科。
化工系现有教职工158人,博士后38人,其中,教授57人,包括1名长江特聘教授、2名
长江学者讲座教授、5名浙江大学“求是特聘”教授、3名国家杰出青年基金获得者、8
名教育部跨世纪优秀人才支持计划和新世纪优秀人才支持计划人才。2010年,我系科研
经费到款超过1亿,其中,国家自然科学基金项目共33项,获批率达46%,获批科技部“
973”项目2项,李伯耿、杨立荣为“973”首席专家。2010年还获国家、省部级科研奖
励9项。我系一贯... 阅读全帖
M*****6
发帖数: 14
25
【国内】500强前三美资石油巨头亚太研发中心招聘Research Scientist
Job Title:
Research Scientist-Vibrational Spectroscopy
Job Requirements:
• Ph.D. in physics, materials science or chemistry
• Strong theoretical and experimental skills in both FT-IR and Raman
spectroscopies, including
familiarity with a wide range of sampling approaches for different material
types
• Hands-on experience utilizing FT-IR interpretation for structural
elucidation
• Demonstrated ability to conduct independent and co... 阅读全帖
b*****r
发帖数: 29
26
来自主题: ChemEng版 - 招化学化工方向的学生啦
代朋友发帖
1. Research Assistantship (RA) for a PhD Student in Mechanical Engineering
An NSF-funded research assistant position is available for a new PhD student
in Mechanical Engineering at the State University of New York (SUNY) at
Binghamton. The research is focused on materials printing using a hybrid
inkjet-electrospray technique. The goal is to print highly-ordered
monolayers of functional nanoparticle inks at high throughput. The student’
s research will be experimental; however, close collab... 阅读全帖
b*****r
发帖数: 29
27
来自主题: ChemEng版 - 招化学化工方向的学生啦
代朋友发帖
1. Research Assistantship (RA) for a PhD Student in Mechanical Engineering
An NSF-funded research assistant position is available for a new PhD student
in Mechanical Engineering at the State University of New York (SUNY) at
Binghamton. The research is focused on materials printing using a hybrid
inkjet-electrospray technique. The goal is to print highly-ordered
monolayers of functional nanoparticle inks at high throughput. The student’
s research will be experimental; however, close collab... 阅读全帖
d***a
发帖数: 73
28
来自主题: ChemEng版 - job opening at Intel
See job description below. Recent college graduate only.
if interested, 请站内联系。
Sr. Materials Engineer
Description
Come join a creative team of Senior Materials Engineers dedicated to the
development of microelectronic packages. Our engineers provide project
management, package design/development, and sustaining support for
integrated circuit or semiconductor assemblies. Our engineers work in cross-
functional teams to define overall package performance and specification and
realize technology ce... 阅读全帖
d***a
发帖数: 73
29
来自主题: ChemEng版 - job opening at Intel
See job description below. Recent college graduate only.
if interested, 请站内联系。
Sr. Materials Engineer
Description
Come join a creative team of Senior Materials Engineers dedicated to the
development of microelectronic packages. Our engineers provide project
management, package design/development, and sustaining support for
integrated circuit or semiconductor assemblies. Our engineers work in cross-
functional teams to define overall package performance and specification and
realize technology ce... 阅读全帖

发帖数: 1
30
诚心求审稿机会,
本人在日本取得硕博学位并有一年半博后经历,目前在美国继续博后工作,已有多次审稿
经验,最近在准备申请美国的绿卡,想继续积攒文章审稿数目,特诚求审稿机会。
主要研究方向有:
Biologically inspired materials;
Polymer biomaterials;
Surface modification;
Living radical polymerization (ATRP/RAFT);
Polymer nanoparticles and hydrogels;
Polymer therapeutics/Gene (RNA/protein) delivery systems;
Cell and material interactions;
Biosensor/biofuel cell;
Nanomedicine / Cancer therapy;
Bacterial, mammal cells and stem cells;
Laser scanning confocal microscopy;
Single cell analysis;
PDMS-... 阅读全帖
i********t
发帖数: 1148
31
来自主题: Chemistry版 - paper help,plz
one more plz
Title: Study of emulsion polymerization of acrylates with a hyperbranched
polymer terminated by unsaturated double bonds
Author(s): Tang MF, Fan XD, Si QF, et al.
Source: ACTA POLYMERICA SINICA Issue: 7 Pages: 680-684 Published: JUL
2007
h**j
发帖数: 24
32
The main responsibilities include:
The successful candidate will assist in the design, development and transfer
of new chromatographic materials into manufacturing. In this hands-on wet-
chemistry position, you will be responsible for preparing new polymeric and
inorganic particles. You will work closely with the Senior Scientists and
interface with a variety of members of the project team.

The successful candidate is likely to have the following required
competencies:
• BS Chemi
w********h
发帖数: 12367
33
来自主题: Chemistry版 - 华尔街日报上面有预测阿。。。
http://online.wsj.com/article/SB10001424052970204683204574358170813148330.html
KRZYSZTOF MATYJASZEWSKI, Professor, Department of Chemistry, Carnegie Mellon
University
Dr. Matyjaszewski developed atom transfer radical polymerization, or ATRP, a
way of combining small molecules called monomers into blocks of polymers,
which are plastics made up of long molecules. This method allows for the
production of a combination of materials with multiple properties that can
be controlled in ways that traditi
x**********m
发帖数: 44
34
《Polymeric Preprints (American Chemical Society Division of Polymer
Chemistry)》
2005, 46(2), 1038
请帮忙发到m*********[email protected].非常感谢
d********O
发帖数: 1196
35
Title: Grafting Reactions of (Hydroxyethyl)-cellulose During Emulsion
Polymerization of Vinyl Monomers
Chapter 18, pp 351–367
Chapter DOI: 10.1021/ba-1986-0213.ch018
Advances in Chemistry, Vol. 213
Thank you very much in advance!
l********7
发帖数: 110
36
来自主题: Chemistry版 - Paper help. Thanks.
Diacetylene polymerization in self-assembled monolayers: influence of the
odd/even nature of the methylene spacer
Polyer, 2000, 41, 8113-8119
q****i
发帖数: 6923
37
来自主题: Chemistry版 - paper help!
1 SILPHENYLENE AND SILPHENYLENE-SILOXANE OLIGOMERS - STRUCTURE-PROPERTY RELATIONSHIPS

more options

Author(s): WERLANG MM, YOSHIDA IVP, DEARAUJO MA
Source: JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS Volume: 5 Issue: 1 Pages: 75-85 Published: MAR 1995
Times Cited: 8 References: 25 Citation Map
2.Organomagnesium compounds in diene polymerization

more options

Author(s): Monakov YB, Marina NG, Duvakina NV, Zolotarev VL
Source: VYSOKOMOLEKULY
d*****3
发帖数: 3702
38
来自主题: Chemistry版 - 这个是大牛么?
Currently, there is one postdoctoral position open in the Xu group.
Specifically, we are looking for an excellent polymer chemist with expertise
in living polymerizations. Experience in protein chemistry is a plus.
Please send your CV to txuucbgmail.com. (6/26/2009)
r******0
发帖数: 2753
39
Normal. My first company was like that. No presentation, only SIMPLE questions.
The hiring manager asked me,"You are a polymer chemist?"
"Yes."
"If I ask you to polymerize MMA, what initiator you would use?"
"!!!!!????? AIBN?"
"what solvent?"
"THF?"
"Good, when can you start?"
"???!!!......."
Basically they only needed a cheap labor. But they hoped they could have a
cheap labor with a brain, just in case they might want a brain sometime.
d****i
发帖数: 360
40
来自主题: Chemistry版 - Please help me with this paper! Thanks!
Cyclodextrin-Based Polymeric Materials: Synthesis, Properties, and
Pharmaceutical/Biomedical Applications
Frank van de Manakker, Tina Vermonden, Cornelus F. van Nostrum and Wim E.
Hennink*
Biomacromolecules, Article ASAP
Thanks a lot!
d****i
发帖数: 360
41
Cyclodextrin-Based Polymeric Materials: Synthesis, Properties, and
Pharmaceutical/Biomedical Applications
Frank van de Manakker, Tina Vermonden, Cornelus F. van Nostrum and Wim E.
Hennink*
Biomacromolecules, Article ASAP
Thanks a lot!
e-mail: x**********[email protected]
x********e
发帖数: 1064
42
来自主题: Chemistry版 - Paper Help (1 baozi)
Optimization of Atom Transfer Radical Polymerization Using Cu(I)/Tris(2-(
dimethylamino)ethyl)amine as a Catalyst

Macromolecules, 2000, 33 (23), pp 8629–8639
please sent to x**********[email protected]
Thanks! bow
w**********g
发帖数: 687
43
来自主题: Chemistry版 - paper help, thank you
Please email to n***************[email protected]
thank you.
Effect of surface treatment of a polymeric filler on its interaction with a
plasticizer
Journal of Applied Polymer Science
Volume 34 Issue 2, Pages 507 - 516, 2003
http://www3.interscience.wiley.com/journal/104028668/abstract?CRETRY=1&SRETRY=0
q****i
发帖数: 6923
44
来自主题: Chemistry版 - 黄宪。。。
了结不了了,i don't think he can make it.我这次acs的presentation要因为没有
nmr的数据丢脸了。表征一个polymerization,只有红外和GPC,叫我怎么讲啊。每次指
导他做实验都是打一个多小时的电话,什么细节都讲到了,还是没有搞出来。我都想干
脆我回去一个礼拜帮他做了算了。
q****i
发帖数: 6923
45
有一个monomer,当然是小分子了,可以spin coat在salt plate 上,也可以夹在salt
plates之间成膜。但是不能spin coat在si wafer上,会dewet。所以没有办法用
ellipsometry 来测厚度。吸收紫外光,并发生化学反应polymerization成oligomer。
据我所知是可以测厚度的,曾经有人告诉过我怎么测,现在忘掉了,一点都想不起来了
。大家帮我想想把。中选了的方法有包子答谢。
能不能用这个吸收紫外的性质来测?用极弱的紫外光?
我必须测膜的厚度来计算它到底吸收了多少光,来算这个紫外光聚合的UV efficiency.
s**m
发帖数: 4340
46
来自主题: Chemistry版 - 10个包子求三篇PMSE Preprints
每篇给3个包子,如果全都找全就一共给10个包子。
谢谢您的帮助!
我的email: c*************[email protected]
Li, K. et al. Dual modification of M13 bacteriophage and its application for
cell imaging. PMSE Preprints (2009), 101 1198-1199.
Li, T. et al. Synthesis of bionanoparticle-polymer conjugates via in situ
atom transfer radical polymerization (ATRP) reaction. PMSE Preprints (2007)
, 97 621-622.
Li, S. et al. Preparation and characterization of polypyrrole nanodisc
templated by tobacco mosaic virus. PMSE Preprints (2007), 96
p*i
发帖数: 1481
47
来自主题: Chemistry版 - Paper help, Thanks!
Polymerization of 3TUTS SAM Formed on ITO Substrates
Molecular Crystals and Liquid Crystals, Volume 471, January 2007 , pages 163
- 171
http://www.informaworld.com/smpp/content~content=a782584607&db=all
Please send to h********[email protected]
thanks a lot!
h*******o
发帖数: 1114
48
来自主题: Chemistry版 - Paper help! Thank you so much!
“Emulsion polymerization of Acrylic Monomers” Bull. CM-104 A/cf Rohm and
Haas Company, Philadelphia, PA
s**c
发帖数: 34339
49
来自主题: Chemistry版 - 求文献
Hybrid polymer latexes acrylics–polyurethane from miniemulsion
polymerization properties of hybrid latexes versus blends
http://www.sendspace.com/file/rq5s7f
d********O
发帖数: 1196
50
来自主题: Chemistry版 - Paper help, Thanks! {Biomacromolecules }
Title:Versatile Grafting of Polysaccharides in Homogeneous Mild Conditions
by Using Atom Transfer Radical Polymerization
Biomacromolecules, 2006, 7 (7), pp 2154–2161
DOI: 10.1021/bm0601373
Please send to D********[email protected] , if this is more convenient for you.
Thanks a lot!
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