模板通过PCR获得 then TA sub cloning, then pick up clone do sequencing for
check the probe sequencing or not?
if not how to prove that PCR product as Dig probe without any mutation
if all above steps were done
then might need to PCR about 1kp product as probe
please refer
Primer design http://pga.mgh.harvard.edu/primerbank/
original hint was from http://www.mitbbs.com/article_t/Biology/31807477.html
19th floor post
