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发帖数: 156 | 3 那个TRC弄出的也称为一代shRNA (by Broad Institue). Hannon, Elledge作的是二代
，as 楼上mentioned, it is based on a natural miRNA structure. Both libraries
have been used extensively to generate lots of high-profile papers。
Generation II的library出的文章似乎要多一些，光 Elledge就好多篇 (such as
Synthetic lethality with RAS, cancer essential genes, tumor suppressor REST
and PTPN12, etc).
顺便问一下，shRNA导致的any phenotype是不是很难去rescue by introducing back
shRNA-resistant gene. 昨天还在看science那两篇cancer essential genes的文章，
似乎都没对任何hit试图rescue。那位大虾解... 阅读全帖 |
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