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w******g
发帖数: 301 | 2 ttaccaccactc -> ttaccactc 的deletion用啥方法genotype比较好？
1. 已经尝试用mismatch primers做PCR，结果mutant primers放大wt， wt primers放
大mutant. Tandem repeat很让人头疼啊。
2. 正在考虑用 IDT 的 ZEN Black Hole quenchers做Taqman, 但是不知道quencher碰
上tandem repeat会出现啥情况。而且那个quencher好贵。
3. 测序就更加贵了，不在考虑中。
4. 片段无法酶切，木有位点。
请教一下高手们，还有其他快速便宜的方法来做么？还有啥我木有想到的么？谢谢 |
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y****6
发帖数: 196 | 5 Thanks for sinister and kaka1000's reply. I labeled the antibody with
fluorescent dyes and quenchers, and I want to digest the antibody to small
fragments to break the FRET between dyes and quenchers. It would be best if
I can digest the antibody into the smallest fragments. Papain may not be the
best choice as it only cut antibody into three pieces. Maybe I can use some
kind of protease mixture? Any suggestion is welcome. |
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l***o
发帖数: 7937 | 6 你是libido quencher。即使你渴望被强奸，但你的长相使你脱光了都让男人立马阳痿。 |
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k********n
发帖数: 18523 | 7 Eating Fruit - this opened my eyes.
Dr Stephen Mak
treats terminal ill cancer patients by
"un-orthodox" way and many patients recovered.
Before he used solar energy to clear the illnesses of his patients.
He believes on natural healing in the body against illnesses.
See his article below.
It is one of the strategies to heal cancer.
As of late, my success rate in curing cancer is about 80%.
Cancer patients shouldn't die. The cure for cancer is already found.
It is whether you believe it or not.
I... 阅读全帖 |
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P****S
发帖数: 2457 | 8 我其实也没说啥做啥，就是让他右脚先上一小步再出手，脚在手先，这样转腰的力可以
用来控制球，如此而已。
就是那个从Costco可以大箱大箱买的 Gatorade Performance G Series Thirst
Quencher。 |
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R****n
发帖数: 708 | 10 syber green原理是用allele specific PCR吧，一个primer 3'端用来区分phenotype.
我没做过不太清楚效果。还有什么和ligation结合的，HRM的。你说的太笼统了。
Taqman是用probe,两边一个dye一个quencher，不同的sequence dye不一样，一管PCR搞
定。这个基本上是业界的标准方法 |
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h*******o
发帖数: 4884 | 11 Taqman probe is fluorescent with a quencher. |
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C******d
发帖数: 362 | 12 reporter dye: 6-FAM
Quencher: TAMRA
I collect data at the end of annealing.
我设定的检测信号：ROX和FAM， ROX is reference
这些天，我就只准备了两个样品，一个NTC，一个添加了cDNA的，看是否能得到扩增曲
线，但结果都总是没有，我还改变了probe的浓度
仪器设置上，你觉得还有哪些应该注意的？谢谢 |
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N**C
发帖数: 74 | 13 title: Significant Expansion of Fluorescent Protein Sensing Ability through
the Genetic Incorporation of Superior Photo-Induced Electron-Transfer
Quenchers
link: http://pubs.acs.org/doi/abs/10.1021/ja505219r
Thank you very much~ |
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c*s
发帖数: 2145 | 14 99年或更早看过kamat的关于CdS/TiO2复合体系的电子转移的文章，法在jpc上，概念清
晰，表述清楚，堪称经典
关于你那个碳管增加一倍，电子密度没有相应增加，是不是因为tio2中可用于转移的电
子树木已定，随着贪官的增多， 也就是quencher增多，转移到每个贪官上的电子束下
降了？ |
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