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全部话题 - 话题: rechip
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k*******n
发帖数: 190
1
看这个:
October 18: Body Ordered; Lens Research Begins
After trying out an A33 at my local Sony Style store and finding the
electronic viewfinder more than acceptable, I decided to order an SLT-A55V (
body only) from Crutchfield.com. Normally, I would have never thought of
Crutchfield as a place to shop for a DSLR camera, so I was surprised to find
out that it was not just the only retailer with A55 bodies in stock, but
also an authorized Sony distributor—perhaps as a result of all the Sony
audio g... 阅读全帖
Z******5
发帖数: 435
2
来自主题: Biology版 - 请教个关于CHIP的问题
检索ReChIP,一大堆文章。 不过这个应该不好做。
D******9
发帖数: 2665
3
想验证一下两个转录因子是否相互作用后结合到同一段DNA上, 谢谢。
k*****n
发帖数: 323
4
re-chip qpcr?没多少人信吧
D******9
发帖数: 2665
5
想验证一下两个转录因子是否相互作用后结合到同一段DNA上, 谢谢。
k*****n
发帖数: 323
6
re-chip qpcr?没多少人信吧
g*******f
发帖数: 427
7
我也觉得这个re ChIP听起来容易,实际上太难,我也试过基本上不可能得到signal。
但是看到Christopher K. Glass 和Michael G. Rosenfeld 这种大牛的paper上那么好
的结果我都不知道他们是怎么做出来的,他们的所有的paper中 ChIP 的结果也都是那
么漂亮,可是我用的同样的抗体,同样的方法,根本重复不出来,总不成写EMAIL 去质
问他们,你们他妈的是怎么弄出来的。
这有两篇文章里的方法给你做个参考
2009-Cooperative NCoR SMRT interactions establish a corepressor-based
strategy for integration of inflammatory
2003-Estrogen Receptor-α Directs Ordered, Cyclical, and Combinatorial
Recruitment of Cofactors on a Natural Target Promoter
D******9
发帖数: 2665
8
谢谢, REVIEWER要这个东东, 没有办法, 只好试试了。
y*********1
发帖数: 46
9
Active Motif has a re-ChIP kit you can try.
h****n
发帖数: 2552
10
我做出来过,很多细节需要你用心去优化,最重要的当然是抗体,就算同一公司同一货
号的抗体也未必一样。
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