k*******n 发帖数: 190 | 1 看这个:
October 18: Body Ordered; Lens Research Begins
After trying out an A33 at my local Sony Style store and finding the
electronic viewfinder more than acceptable, I decided to order an SLT-A55V (
body only) from Crutchfield.com. Normally, I would have never thought of
Crutchfield as a place to shop for a DSLR camera, so I was surprised to find
out that it was not just the only retailer with A55 bodies in stock, but
also an authorized Sony distributor—perhaps as a result of all the Sony
audio g... 阅读全帖 |
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Z******5 发帖数: 435 | 2 检索ReChIP,一大堆文章。 不过这个应该不好做。 |
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D******9 发帖数: 2665 | 3 想验证一下两个转录因子是否相互作用后结合到同一段DNA上, 谢谢。 |
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D******9 发帖数: 2665 | 5 想验证一下两个转录因子是否相互作用后结合到同一段DNA上, 谢谢。 |
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g*******f 发帖数: 427 | 7 我也觉得这个re ChIP听起来容易,实际上太难,我也试过基本上不可能得到signal。
但是看到Christopher K. Glass 和Michael G. Rosenfeld 这种大牛的paper上那么好
的结果我都不知道他们是怎么做出来的,他们的所有的paper中 ChIP 的结果也都是那
么漂亮,可是我用的同样的抗体,同样的方法,根本重复不出来,总不成写EMAIL 去质
问他们,你们他妈的是怎么弄出来的。
这有两篇文章里的方法给你做个参考
2009-Cooperative NCoR SMRT interactions establish a corepressor-based
strategy for integration of inflammatory
2003-Estrogen Receptor-α Directs Ordered, Cyclical, and Combinatorial
Recruitment of Cofactors on a Natural Target Promoter |
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D******9 发帖数: 2665 | 8 谢谢, REVIEWER要这个东东, 没有办法, 只好试试了。 |
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y*********1 发帖数: 46 | 9 Active Motif has a re-ChIP kit you can try. |
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h****n 发帖数: 2552 | 10 我做出来过,很多细节需要你用心去优化,最重要的当然是抗体,就算同一公司同一货
号的抗体也未必一样。 |
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