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l***p
发帖数: 177 | 2 鎴戝凡缁忕敤oil base鐨刾rimer鐩存帴娑備笂浜 |
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u*********e
发帖数: 9616 | 3 德科，巴拉克,贝莱蒂不都走了么，还买了Rimeres.还想怎么着？ |
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a****e
发帖数: 173 | 4 How can my Muse want subject to invent,
While thou dost breathe, that pour'st into my verse
Thine own sweet argument, too excellent
For every vulgar paper to rehearse?
O, give thyself the thanks, if aught in me
Worthy perusal stand against thy sight;
For who's so dumb that cannot write to thee,
When thou thyself dost give invention light?
Be thou the tenth Muse, ten times more in worth
Than those old nine which rimers invocate;
And he that calls on thee, let him bring forth
Eternal numbers to ou |
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a*****g
发帖数: 543 | 5 what kind of Cre did you use?
If you happened to have SOX2-Cre, you will be able to get good KO( of target
ed site).
I'm assuming you are doing appropriate control of the genotyping, with both
WT primers and recombinant primers...
How big was the targeted exon? where is your qRT-PCR targeting? Having one p
rimer locating in the exon may help to clarify whether deletion of the exon
occurred... |
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