x*****d
发帖数: 704 | 1 Qiagen Fibrous Tissue kit自带一个小的DNase kit，要做DNase digest的。你的RIN
多少？ 也有可能是有降解。 |
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a*******g
发帖数: 33 | 2 最好的一个是8.4，很多都无法计算，只有一条带
RIN |
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x*****d
发帖数: 704 | 3
一般RIN 7以上就可以了. 你这个只有一条带也有可能是Agilent的Chip有问题. 要是怕
降解就用RNA Later ICE试试. |
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z**********8
发帖数: 766 | 4 从微量培养细胞中抽提RNA，没有问题，RIN大于9.2
从微量病人骨髓分选细胞中抽提，少量介于8-9，大多3-8，质量不足以继续测序（for
RNAseq）。
Nanodrop测的浓度都介于20-几百ng/ul，现在浓度不是我最大的问题，RNA降解造成的
质量问题无法解决。
尝试了qiagen的两个kit： Allprep DNA/RNA/Protein 80004；Allprep DNA/RNA 80204
，两个没有大的区别，都是从培养细胞中没问题，抽提病人样品就不行。在犹豫是不是
只能用RNAeasy kit？
问了好几个qiagen的tech support，也分别尝试了他们的方法，均不能提高RNA质量
请教哪位达人有私人珍藏经验可以分享一下吗，多谢了！ |
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a*******g
发帖数: 33 | 5 大家好，我最近在做RNA seq。样品来自于激光剪切老鼠组织，RIN只有6左右，量也不
多，用了150ng total RNA.True seq V3建的library，qPCR显示library量够多。用
highseq 2500测了，在highthrough output条件下，4个样品在一个lane里，结果每个
样品只得到了10-15M 2X100bp，远低于预计的30M。core facility说他们也不知道原因
。请问大家有什么经验可以分享吗？另外，有没好的测序服务中心推荐呢？ |
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a*******g
发帖数: 33 | 6 rna定量是用qubit，library是用qpcr，他们说只能这样定量分析了.激光剪切的老鼠冰
冻组织提取Rna，RIN值大家一般能得到多少?有没有好的protocol可以分享 |
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c*z
发帖数: 157 | 7 RIN有6已经不错了
fresh frozen LCM关键是切下来立马要进lysis buffer，不过你这个case这些都不是问题
如果core没做错什么的话。。
要不。。truseq。。难道3’没处理好或者adaptor没连好？ |
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c*******l
发帖数: 4801 | 8 这俩区别怎么想？
当然一个interverting,一个non-inverting
两个的gain好像基本差不多，Rout基本差不多
输入Rin呢，可能non-interverting小点？？
frequency response大概non-inverting好点因为没gate电容？
thanks
怎么来比较common source/common source degeneration的优缺点？
thanks thanks |
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n*****y
发帖数: 134 | 9 C-S C-G
voltage gain
Rin
Rout
max vin swing to keep device in sat.
easeness of DC biasing
frequency response
......
then why typical amplifier, especially last stage of amp, it is C-S,
not CG, could u explain? :-) |
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c*******l
发帖数: 4801 | 10 可能是因为虽然gain差不多，Rout也差不多，但是Rin的话，还是CS最大，所以好吧？？
卡卡 |
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c*******l
发帖数: 4801 | 11 gain差不多
rin是CS大
Rout也差不多
max vin的话，CS方便，因为S接地，但是如果CG的话，swing上线离gate至少要Vt，所
以不好
ease的话，还是CS方便些。要不G如果接低电压，S就得swing到negative去了
frequency的话，CG好，因为cap小，但是output stage估计多的是low freq了，
不要这个benefit?
嘿嘿 |
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C*******g
发帖数: 82 | 13 for RF applications Rin is not a problem since people can use matching
network or transformers to tune the input impedances so that optimal power
gain can be achieved. Besides sometimes people use the 1/gm property of CG
stage to form a wideband input match to 50Ω.
In low frequency designs when people just connect the input of the second
stage to the output of the first stage it is a problem if the input
impedance of the following stage is too low. |
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ET
发帖数: 10701 | 14 这里的一个重要区别是考虑high frequency, capacitive load
要看impedance,而不只是低频下的resistance.然后用 r*c来近似一个pole.
实际上，也可以假设output resistance 很大，无穷来简化。
你是从vin->vout ,在m3 的gate端的断开的吗？
比如你的boosting amplifier，有Gm, smallest Rout & infinite Rin , ideal opamp
.
从vin, 在m1的输出有 v2, v2=gm1*vin/sc1, 可以画一个current source:gm1*vin //
c1
v2控制boosting amplifier & cascode amplifier
boosting amplifier, 可以画成gm3*v2 //c3， 它有输出电压：v4=gm3*v2/sc3
再看cascode transistor, 它的current source是gm2*vgs , here vgs=v4-v2, 这个cu
rrent source // c3
所以，vout= |
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b********n
发帖数: 181 | 15 见图
答案我在图里抄了，从书后面的附录里抄的。无奈没找到分析过程
我直观的看，也就能看出来Rin是1/gm^2/rds
请问大牛，这个3.25是怎么算的 |
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s*****n
发帖数: 2 | 16 1) Without feedback, assume the impedance looking up at the source of M3 is
RD, then the impedance looking at the source of M3 is
Rin = (RD+Ro3)/(1+gm3*Ro3) = (RD+Ro3)/(gm3*Ro3)
2) M1 senses the Iout (Iout*RD) and feedback the current to input node gm1*
RD*Iout, loop gain T = gm1*RD
3) shunt feedback at the input --> Rin_eff = (RD+Ro3)/(gm3*Ro3*gm1*RD) (
ignore the 1+T factor)
4) Now look at the RD, 坑爹, depends on the load impedance:from the fig,
assume no loading,
RD = Rdsp || (gmn*Rdsn^2+Rds... 阅读全帖 |
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s**g
发帖数: 66 | 17 assume one port resistor network,
total input res Rin
one element res Rx
input current Iin
current flow in Rx branch Ix
It can be proved:
sensitivity S = dRin/dRx = Ix^2/Iin^2 >= 0
Vallese "Incremental versus adjoint models for network sensivity analysis"
TCAS 1974 |
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W******g
发帖数: 143 | 18
Please see this I got from MKSAP 15. BCG in Children seems very reasonable
in China and India.
The bacille Calmette-Guérin vaccine is derived from an attenuated strain of
M. bovis and is used worldwide except in the United States. The efficacy of
this vaccine for preventing serious forms of tuberculosis (tuberculous
meningitis and miliary tuberculosis) has been evaluated in recent meta-
analyses and has been shown to be high in children (>80%). However, this
protective effect for pulmonary tube... 阅读全帖 |
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f*****i
发帖数: 3558 | 20 木材
放假去看了几家买木材的地方.分享一下结果
EarthSource Forest Products
1810 S 7th St
San Jose, CA 95112
(408) 288-7257
www.earthsourcewood.com/
8:00 am to 4:30 pm Mon. - Fri.
一般用的plywood都有,一些on sale的hardwood 价格很好.
GLOBAL WOOD SOURCE, INC
1698 ROGERS AVENUE, SAN JOSE, CA 95112
MAIN NUMBER : 1-(408)-392-0573
FAX NUMBER : 1-(408)-392-0574
REGULAR STORE HOURS:
MONDAY TO FRIDAY : 8AM TO 5PM
SATURDAY : 8AM TO 2PM
SUNDAY : CLOSE
有很多各式各样的hardwood,非常多的品种,很开眼界.如果你找特殊的木材,可以去那里
试试.plywood没有什么选择.
Mac Beath Hardwood Co
2211 Rin |
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w*******y
发帖数: 60932 | 22 Work's on hold for an hour so I decided to look for cheap anime-related toys
on amazon that are eligible for Free Super Saver Shipping and free shipping
with Prime. Some of these items might be reposts, but ALL of them aren't.
Also, some of these toys are limited in stock so hurry. You might be able to
find some of this stuff for the same price on Rightstuf.com, however, you
don't get free 2-day shipping (assuming you have Prime). Feel free to add to
this post...or not...most of this stuff is cr... 阅读全帖 |
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w*******y
发帖数: 60932 | 23 Here is the link: Link:
http://www.walmart.com/browse/Movies/_/N-7316?path=0:4096:53059 &depts=Mo
Here is the list:
Going The Distance (Widescreen)
The Lord Of The Rings: The Return Of The King (Widescreen)
Fool's Gold (Widescreen)
Heat (Widescreen)
The Last Samurai (Widescreen)
He's Just Not That Into You (Widescreen, Full Frame)
The Bourne Ultimatum (Full Frame)
$2.50 List:
Adventure Collection: Aladdin / Jungle Book / White Fang / Pinocchio / Leo
The Lion: King Of The Jungle (Full Frame)
Viet... 阅读全帖 |
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m*********k
发帖数: 10521 | 24 本次统计截止时间为：2013-03-18 02:00:00 （美东时间）
成功奖励 20 伪币的用户: alassea, AngelCity, bufanle, CDSWJ, chitang, choming
, dgbsv, dopp, dorashat, fishing66, gamor, garphy, GiovannaX, guali,
happyboar, HQ, intercomp, laufine, ldy, luowei, metalmetal, MO2007, moha,
nolovefate, PatekPhilip, pigeona, pingpingpang, powerpower, qingshi601,
qiuXX, rainbowfairy, Regina, ricst1985, sammix, SpingRain, talkfact, tnc,
Trotsky, Visitation, wilfredwang, woshizhangci, xiaod2046, yellowapple,
zcjsword
奖励版面:(bagua)20伪币成功
奖励版面:(... 阅读全帖 |
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s*****t
发帖数: 1994 | 25 Enceladus Creates Saturn's E Ring
Credit: CICLOPS, JPL, ESA, NASA
Explanation: The active moon Enceladus appears to be making Saturn's E ring.
An amazing picture showing the moon at work was taken late last year by the
Saturn-orbiting Cassini spacecraft and is shown above. Enceladus is the
bright point near the center, right near the center of Saturn's E ring.
Streams of ice and water vapor can be seen pouring off Enceladus into the E
ring. The above bright image of the normally faint E-ring was |
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