s**********8
发帖数: 25265 | 1 那是忽悠你 价钱高 trap的dymanic range 没有triple Q 的好 对定量是个死穴 |
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l*****f
发帖数: 13466 | 2 多谢哈。不过 4000 qtrap 我觉得还好？
我赶紧让他们给5000的quote吧。Thanks! |
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d******s
发帖数: 674 | 3 能展开说说为啥QTRAP没有3Q的定量好？俺觉得QTRAP 本质上就是3Q，只是最后一个Q加
了俩voltage barrier。当做3Q来用的话跟3Q没啥区别。
最近做了个project，小分子定量，在T家的QUANTUM跟在AB 家的QTRAP上做（当然是用
在3Q模式），dynanmic 都还好，比较来说都差不多。
俺倒是很同意5500不值得花那么多钱，不过应该比5000灵敏度会高一些（只是听说，没
自己做过比较，所以也不好下定论），加上EPI， MS3等等附加的trap功能，如果有钱
没地方花的话，俺也不介意弄一个在实验室玩玩，呵呵。 |
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f********e
发帖数: 2194 | 4 5500是qtrap，不是trap，比纯qqq好，灵敏度极高，U形CC，省体积
定量很好，不过由于灵敏度太高经常要稀释样品，hehe |
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s**********8
发帖数: 25265 | 6 说的就是这个 trap的dynamic range. |
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f********e
发帖数: 2194 | 7 我说错了，是U形collision cell。
QTrap的trap是可作Q，也可做trap，所以没有你说的问题 |
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s**********8
发帖数: 25265 | 8 qtrap 是U型的？ thermo varian啥的是吧 |
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f********e
发帖数: 2194 | 9 只有abi的5500的collision cell是U形的 |
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L****r
发帖数: 333 | 10 Applied Biosystems 4000 Q TRAP
The 4000 Q TRAP® combines triple quadrupole scan modes with the
advanced features of MDS SCIEX Linear ion trap scans in a single LC/MS/MS
run that identifies, characterizes, and confirms Phase I and II metabolites.
The system includes three sources—an ESI (IonSpray), APCI (Heated
Nebulizer), and NanoSpray—a vacuum pump, and a PC with Analyst 1.4.1
software.
Applied Biosystems 4000
The API 4000 combines a Liquid Chromatography (LC) Mass Spectrometer (MS)
with a... 阅读全帖 |
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i***l
发帖数: 9994 | 11 Sciex and superstic, 二位能不能给推荐一本书或者综述，讲解如何做标准曲线，QC
和Validation的？多谢。 |
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p*******8
发帖数: 158 | 12 Our lab plans to buy one, but not sure how much budget will need for regular
-use basis. So can you tell me approximately how much for a simple MALDI or
MALDI-MS/MS, based on your experience. I know I can quote with different
companies such like AB Sciex, Bruker, or Micromass. However, I won't be
bothered by their agents right now during this beginning step . By the way,
tell me instrument type and specifications as possible as you can. Thanks. |
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y*****s
发帖数: 1047 | 13 造质谱的？depends on the type
thermo, sciex, waters, agilent, bruker ... |
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S***x
发帖数: 2012 | 14 是波士顿附近的一个制药公司，需要找一个entry/mid level scientist. 属于
bioanalytical group,small molecule quantitation, using LC-MS/MS. 这个职位是
个lab position, 要求有sample preparation 和 method development的经验，如果有
discovery or in-vitro or instrument maintenance 的经验更好.
Prefer BS/MS level with 2-5 years industry experiences. AB Sciex Mass Spec,
Shimadzu and Waters UPLC experiences. Working at CRO and GLP experiences are
plus. 最好是local candidate.
如有意申请，请站内联系 或者 email to：p***[email protected]. 谢谢！ |
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z**4
发帖数: 37 | 15 Top 2 MS instrument company: Thermo Electron, AB Sciex
Agilent, Shimazu, Waters are especially good at chromatography. |
|
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c******m
发帖数: 884 | 17 Never heard about it. Is it made by PE or Sciex? single MS? |
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b*******g
发帖数: 1309 | 18 你这个应该叫transmission efficiency，QQQ,从形成离子进入Q1开始算，到Q3，效率
超过90%，好像在95%以上，所以要提高LOD必须在ionization source 跟Q0上下功夫。
举个例子AB Sciex 的6500跟5500 的区别就是source enhance 2倍LOD，Q0（Qjet)
enhance 2倍2LOD，共计4倍LOD
QTOF 的ion transmission主要是损失在 flight 过程中，另外TOF的pulse frequency
并不慢，大部分仪器都在 20kHz 左右，就是0.05ms一次。
速度慢在仪器跟电脑之间的传输，没想到吧，每个TOF的spectrum在被digitization过
后从instrument到电脑需要的时间在ms ，这段时间TOF只能idle等digitizer
clearance
所以QTOF效率取决于duty cycle time跟飞行过程中的离子损失。QQQ现在的duty cycle
一
般可以到2-5ms per MRM，但是QTOF最快能到10ms，一般只能到50ms。意味着同样的时... 阅读全帖 |
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l*****u
发帖数: 1788 | 19 CRO，wiki一下很难吗？
这都懒得动手真的不能抱怨工作不好找啊...
contract lab,美国很多，和外包有啥关系。
------
发信人: Sciex (LCMSMS), 信区: Chemistry
标 题: Re: 现在Fresh phD薪资行情坏到这个地步了
发信站: BBS 未名空间站 (Thu Feb 21 22:13:46 2013, 美东)
外包公司 |
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l*****u
发帖数: 1788 | 20 CRO，wiki一下很难吗？
这都懒得动手真的不能抱怨工作不好找啊...
contract lab,美国很多，和外包有啥关系。
------
发信人: Sciex (LCMSMS), 信区: Chemistry
标 题: Re: 现在Fresh phD薪资行情坏到这个地步了
发信站: BBS 未名空间站 (Thu Feb 21 22:13:46 2013, 美东)
外包公司 |
|
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u*********t
发帖数: 45 | 22 各位LC/MS/MS高手：
实验室准备买一台二手LC／MSMS。主要用于杀虫剂定量检测，有时也要用于寻找和签定
未知化学物质。
知道agilent的三级四杆质谱仪在定量方面做得很好，但和其它公司三级四杆质谱仪一
样，定性方面不强。也有推荐AB Sciex 的QTrap。还有WATERS的QTOF。
希望板上高手给予指点。谢谢了。 |
|
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s*******c
发帖数: 179 | 25 If quantitation is major task, go for Sciex Qtrap. If ID is major task, go
for Thermo QE. |
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g*****x
发帖数: 3283 | 26 做proteomics直接上qexactive
metabolomics上sciex的qqq吧 |
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x**********0
发帖数: 33 | 27 POSITION SUMMARY
A Senior Scientist who will play a key role in new drug characterization and
analysis using LC/MS in research and development. Major responsibilities
include bioanalytical assay development for drug DMPK studies and peptide/
protein and antibody analysis in support of drug candidate selection,
preclinical and IND-enabling studies
ESSENTIAL FUNCTIONS
- Peptide/protein and antibody analysis using Agilent QTOF
- Developing in vitro DMPK assays using SCIEX QTRAP including meta... 阅读全帖 |
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S***x
发帖数: 2012 | 28 【 以下文字转载自 Chemistry 讨论区 】
发信人: Sciex (LCMSMS), 信区: Chemistry
标 题: Used API 4000 Qtrap LC-MS/MS for sale
发信站: BBS 未名空间站 (Thu Jul 23 21:04:47 2009, 美东)
包括安装和第一年的保修
感兴趣的话，请发站内邮件给我 |
|
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c**********e
发帖数: 694 | 30
原来是Thermo啊，我一直以为他家的待遇是较高的。Thermo, AB Sciex, Agilent,
Waters,是不是就后两家比较厚道啊。 |
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x**********0
发帖数: 33 | 31 POSITION SUMMARY
A Senior Scientist who will play a key role in new drug characterization and
analysis using LC/MS in research and development. Major responsibilities
include bioanalytical assay development for drug DMPK studies and peptide/
protein and antibody analysis in support of drug candidate selection,
preclinical and IND-enabling studies
ESSENTIAL FUNCTIONS
- Peptide/protein and antibody analysis using Agilent QTOF
- Developing in vitro DMPK assays using SCIEX QTRAP including meta... 阅读全帖 |
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m*********k
发帖数: 10521 | 32 [LoveNLust] aihaha Jan 28 ● 1/28, 发包子，22个
10*22*1.1=242
成功奖励 10 伪币的用户: AnakinVader, beegeintar, bigdwarf365, Breuvial, churchance, curjasong, Fogell, ForzaRui, GUIHUA, heisejinyao, hinterlandy, loriblu, maomaopao, mj0, paomaomao, pH5, Sciex, subgirI, substitution, ttzzleg, withorchid, ZhangJiZhong
成功扣除 242 伪币的用户: aihaha |
|
m*********k
发帖数: 10521 | 33 [LoveNLust] ZhangJiZhong Feb 23 ● 我发些包子
10*20*1.1=220
成功奖励 10 伪币的用户: ADOMET8, AnakinVader, BG, churchance, curjasong, elvinGG, Fogell, glorias2009, GoBeijingGo, hangonthere, hinterlandy, kungfu08, lipoigm, maopaopao, NWWolf, oriolechao, ppestress, rednosewolf, Sciex, snowyrose, substitution, ttzzleg, upupupu
成功扣除 220 伪币的用户: ZhangJiZhong |
|
m*********k
发帖数: 10521 | 34 [LoveNLust] aihaha Mar 12. ● It is the time.
10*40=400
成功奖励 10 伪币的用户: aihaha, bigdwarf365, Breuvial, cengceng, churchance, EternalLife, flowerimm, GoBeijingGo, hangonthere, haobahaoba, maopaopao, NWWolf, Sciex, Shenhu, snowyrose, solosoul, subgirI, substitution, ttzzleg, ZhangJiZhong
成功扣除 400 伪币的用户: aihaha |
|
m*********k
发帖数: 10521 | 35 [LoveNLust] makiyo Apr 13 ● 包子结束，大家等着吃，为橙子
10*30*1.1=330
成功奖励 10 伪币的用户: ADOMET8, alibaba0819, Breuvial, bulawann, cengceng, churchance, crashinfo, cymene, Fogell, gaginang, GoBeijingGo, hangonthere, haobahaoba, kungfu08, lipoigm, makiyo, maopaopao, melonbaby, newsun, NWWolf, Sciex, Shenhu, snowyrose, substitution, ttzzleg, uhopepod, wayelsha, WBIlove, WindyHan, xiaochuzi
成功扣除 330 伪币的用户: makiyo |
|
N****f
发帖数: 25759 | 36 注：1）版面活动：手续费无；2）代发包子：手续费10%:
2
代发版面/ID:
NWWolf
代发事由(主题标题或链接):
“多谢支持！”
奖励/代发包子数量:
以下ID每人10伪币：
churchance
Patchouli
churchance3
Shuaia
awaterlily
wstwst
Shenhu
AprilBreeze
Breuvial
parfume
substitution
aagent
withorchid
aihaha
ADOMET
cengceng
Grammostola
hangonthere
GoBeijingGo
ChenSheng
winging
ColinFirth
YeahPussy
monkeylady
cryingbeg
Sochi2014
ADOMET8
reikoreiko
miami911
xinggaochao
mitbbsFBI
Rio2016
Brazil2014
nycghost
UnderArmour
SST
USA2008
Zumba
beegeintar
HappyandBusy
airdragon77
TenderOnc... 阅读全帖 |
|
m*********k
发帖数: 10521 | 37 [WBCenter] NWWolf Nov 18 ● 申请代发包子
10*68=680
成功奖励 10 伪币的用户: churchance, Patchouli, churchance3, Shuaia, awaterlily, wstwst, Shenhu, AprilBreeze, Breuvial, parfume, substitution, aagent, withorchid, aihaha, ADOMET, cengceng, Grammostola, hangonthere, GoBeijingGo, ChenSheng, winging, ColinFirth, YeahPussy, monkeylady, cryingbeg, Sochi2014, ADOMET8, reikoreiko, miami911, xinggaochao, mitbbsFBI, Rio2016, Brazil2014, nycghost, UnderArmour, SST, USA2008, Zumba, beegeintar, HappyandBusy, air... 阅读全帖 |
|
N****f
发帖数: 25759 | 38 多谢！
P.S. 这个本该是私人发包子，不过没关系，再给版面补上就是。
awaterlily, wstwst, Shenhu, AprilBreeze,
Breuvial, parfume, substitution, aagent, withorchid, aihaha, ADOMET,
cengceng, Grammostola, hangonthere,
GoBeijingGo, ChenSheng, winging, ColinFirth, YeahPussy, monkeylady,
cryingbeg, Sochi2014, ADOMET8,
reikoreiko, miami911, xinggaochao, mitbbsFBI, Rio2016, Brazil2014, nycghost,
UnderArmour, SST, USA2008,
Zumba, beegeintar, HappyandBusy, airdragon77, TenderOnce, Claike, steeleye,
maomao: pao, sophiashs,
daxue, subgirl... 阅读全帖 |
|
N****f
发帖数: 25759 | 39 注：1）版面活动：手续费无；2）代发包子：手续费10%:
1）版面活动：手续费无
代发版面/ID:
LoveNLust
代发事由(主题标题或链接):
薄荷感恩节“说话”包子
伪币金额:
请给以下ID各发伪币十元：
Cengceng
AprilBreeze
Deuse
Wstwst
GoBeijingGo
Sophiashs
Awaterlily
Churchance
ADOMET8
Aagent
Substitution
LiWan
Fenghuolun
USSYorktown
Paomaomao
Zilda
Sciex
Skycaptain08
Yizhitumao
FSH
Winging
NWWolf
Hebrews
Wh
GUIHUA
奖励/代发包子数量:
共计250伪币，请从版面小金库扣除。
谢谢！ |
|
m*********k
发帖数: 10521 | 40 [WBCenter] NWWolf Dec 3. ● LoveNLust（文情影色）版申请代发包子
10*25=250
成功奖励 10 伪币的用户: cengceng, AprilBreeze, deuse, wstwst, GoBeijingGo, sophiashs, awaterlily, churchance, ADOMET8, aagent, substitution, LiWan, fenghuolun, USSYorktown, paomaomao, zilda, Sciex, skycaptain08, yizhitumao, FSH, winging, NWWolf, Hebrews, wh, GUIHUA
扣除版面:(LoveNLust)250伪币成功 |
|
N****f
发帖数: 25759 | 41 多谢！
sophiashs, awaterlily,
churchance, ADOMET8, aagent, substitution, LiWan, fenghuolun, USSYorktown,
paomaomao, zilda, Sciex,
skycaptain08, yizhitumao, FSH, winging, NWWolf, Hebrews, wh, GUIHUA |
|
m*********k
发帖数: 10521 | 42 [LoveNLust] Amiya Jan 17 ● 代瑟瑟发告别贴
10*42=420
成功奖励 10 伪币的用户: NWWolf, doublemilk, santorini, Songp, Aaliyah, hangonthere, ilyfcarrie, txkman, Pachelbel, makiyo, Shenhu, JJ99, Sciex, Fogell, ADOMET8, maomaopao, Meixing, Guyver, oengoeng, YeahPussy, LEAUPARKENZO, ChenSheng, vonphoebe, JackBlack, yizhitumao, VMET, laocat, RazorEdge, wdkong, zhouyu1016, rainbow26, donthurtme, sophiashs, Aplusplus, COSC, zhenghai82, subgirl, simple11, NWWolf, bigpigbig, ColinFirth, hateorlove
扣除版面:(LoveNLus... 阅读全帖 |
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N****f
发帖数: 25759 | 43 Thanks!
hangonthere, ilyfcarrie, txkman,
Pachelbel, makiyo, Shenhu, JJ99, Sciex, Fogell, ADOMET8, maomaopao, Meixing,
Guyver, oengoeng,
YeahPussy, LEAUPARKENZO, ChenSheng, vonphoebe, JackBlack, yizhitumao, VMET,
laocat, RazorEdge,
wdkong, zhouyu1016, rainbow26, donthurtme, sophiashs, Aplusplus, COSC,
zhenghai82, subgirl, simple11,
NWWolf, bigpigbig, ColinFirth, hateorlove |
|
m*********k
发帖数: 10521 | 44 [Music] kim Jan 26 ● 当当当，新版主板斧上任双黄包~~~
20*50*1.1=1100
成功奖励 20 伪币的用户: Feller, kangte, Vieux, lindaing, dusk, macrocat, letwave, ADOMET, linger0223, bluesky02, Rachelle, Sciex, PInce, cengceng, santorini, Guyver, xiaoputi, guoguo82, Teller, daisychan, moonpolar, Pachelbel, surelife, Raininglotus, Songp, skyblue0, sdfarmer, ilyfcarrie, foolsgold, SST, Carrpool, zilda, HifoCH, fengerjing, gnrhrules, suhang, churchance, lllyyyt, Shuaia, haohaidong, shashasha77, airdragon77, ocla2008, Febaug... 阅读全帖 |
|
k*m
发帖数: 1664 | 45 多谢
letwave, ADOMET, linger0223, bluesky02, Rachelle, Sciex, PInce, cengceng,
santorini, Guyver, xiaoputi, guoguo82, Teller, daisychan, moonpolar,
Pachelbel, surelife, Raininglotus, |
|
m*********k
发帖数: 10521 | 46 [Stock] kbrhouston Feb 14 ● 发400个包子，然后闭关去了。(包子数从300增加
10*400=4000
成功奖励 10 伪币的用户: DreamingBird, JianlianYi, hjxds, yyman, electronspin, uscpa, upload, wgaggassi34, JSIS, watermoon, Jingqg1979, AST, qiuyueshifu, PCC, carhunter, linger0223, phila, increasing, JN123, cplus2009, zlzlzl, zkjeffrey, ADOMET8, focus8, Bearhead, bia, lonelypotato, taroman, boshihou2, hellod, myricky, piscesstar, HeeHaw, srtp, ifu06, qingge, mikeandlily1, TEXANS, luanyiking, lillblues, CottonnCandy, zgl, doit, hlwn, fkj... 阅读全帖 |
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m*********k
发帖数: 10521 | 47 [LoveNLust] makiyo Mar 13 ● 发1000伪币，为灾民们祈福
10*93*1.1=1023
成功奖励 10 伪币的用户: ucsb, maopaomao, deuse, ADOMET8, Belichick, waitfortaxi, reikoreiko, RedShark, pestee, skycaptain08, SimoneVerdi, ganoderma, lillblues, Shenhu, JianlianYi, eddyjp, meflying, hangonthere, eddyqj, Mutombo, wstwst, makiyo, LALKobe, BearGrylls, penhold, oengoeng, sophiashs, linger0223, GoBeijingGo, bigday, donthurtme, Rachelle, Shuaia, wonderfully, Meixing, ghrelin, zhetengren, libo, FSH, MC4, zkjeffrey, callalilly, ... 阅读全帖 |
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m*********k
发帖数: 10521 | 48 "[Boston]
sonatina99 Apr 24 ● 发包子(截止啦"
成功扣除 1100 伪币的用户: sonatina99
成功奖励 10 伪币的用户: alive, newprinter, tyning, Seashores, Belichick,
milanese, pfinder, creek6, lemonde, kc, MYFISH, shaoshuminzu, shallow,
patrickcp, bIackwhite, wennyzhang49, azure4068, wokaowokao, gufengc, shark,
hotiz, Jijiwai, gooddeals, liebedich, masterwu, edge, bostontennis, zhnfs,
howdoyoudo, flyingice, feifeifanfan, TeamCancer, GIDP, yqwen, antena,
canhelp2007, gocanucks, dragonren, paopaodou, am1, Julliana, likeme, Kaira... 阅读全帖 |
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N****f
发帖数: 25759 | 49 注：1）版面活动：手续费无；2）个人申请：手续费10%:
1
代发版面/ID:
文情影色（LoveNLust）
代发事由(主题标题或链接):
http://www.mitbbs.com/article_t/LoveNLust/454773.html
赞助（有：附链接，没有：无）:
无
楼主是否计入统计:
是
奖励金额:
所有回帖ID各发10伪币（重复回帖不计；至51楼为止），并另外奖励本版
历代水枪如下：
aagent 40
ADOMET8 60
aihaha 60
aihuahua 20
airdragon77 40
Appreciating 20
bigbendan 20
bigdwarf365 20
biliantian 40
bmwcar 20
Breuvial 20
Buhuai 60
bullpop 20
cengceng 20
ChenSheng 20
childheart 60
choo 60
churchance 40
Claike 20
dahuzi22 20
daisychan 20
deuse 80
DingXiaoLiu 40
draemdraem 20
eu... 阅读全帖 |
|
m*********k
发帖数: 10521 | 50 "[LoveNLust]
NWWolf Dec 9. ● 震天兄提前摆下节日盛宴，有请各位旧雨新知入席！"
成功奖励 20 伪币的用户: aihuahua, Appreciating, bigbendan, bigdwarf365,
bmwcar, bullpop, cengceng, ChenSheng, Claike, dahuzi22, daisychan,
draemdraem, fenghuolun, Fogell, Forever27, FourthMRR, GoBeijingGo,
hangonthere, haobahaoba, Healy, hocc, huhuzhuwang, hwfuture, isimifi,
jadefox, linger0223, loveshopping, lovewind, maomaopao, maopaomao, marywawa,
Meditation, Mright, muxurou, mykua, nhnonentity, nn2, nogard, nudu, NWWoIf,
onearcat, Pachelbel... 阅读全帖 |
|
