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c****1
发帖数: 457 | 2 why not reraise the flop and take control of the betting sequece? I am ready
to go go all in with that monster flop |
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p*******r
发帖数: 4048 | 3 I will supply you with among the best deep sequecing, epigenomics, and in
vivo superresolution imaging techniques in the world. |
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a****o
发帖数: 1786 | 4 Helicossingle 是single molecule sequencing， 是NGS，还没挣钱
PacBio应该算三代， single enzyme, single molecule sequecing |
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a****o
发帖数: 1786 | 5 Greg Hannon shared the story in HMS on Monday. This array-based capture
method is so useful for sequecing ancient DNA. |
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C*******h
发帖数: 75 | 6 1) RT-PCR not quantitative
2) for q-PCR, you would need to have several proper controls at least two
genes as your reference
3) I would suggest you sequece the whole region since you have approximately
30 kb.
4) For certain region with low GC content, you can amplify 5-6 kb fragments
(it is should be easy in most cases) to overcome the PCR obstacles and then
design primers for sequencing.
Good luck |
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f*********e
发帖数: 1144 | 7 if it is a purified protein, try N-terminal sequecing if possible |
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d****7
发帖数: 109 | 8 Glycogen绝对没问题，不论是sequecing还是microarray都不影响
pyrsequencing
DNA |
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m*********D
发帖数: 1727 | 9 哈哈，是啊，sequencing gel跑了多年，压那些X-光片，都是美好的回忆。
读博士的时候，在国内给Bio-rad纯化一种高温DNA polymerase，能解决sequecing反应
时不少二级结构的问题，还挣过几千外快呢。S-35是后来的吧，记得前面用p-32，后来
是P-33，再到S-35。
NEB的catalog确实是一本分子克隆的好书。在国内虽然是作分子生物学出生，克隆没少
作，但来美国作博士后，要作knockout construct时，才真正变成cloning expert：
pUC18里要塞进一个20kb的片断，找合适的restriction enzyme都困难呀。记得当时就
一个Not I是识别八个bp的酶，很有用，另一个是Sal I。Sal I是识别六个bp,但在老鼠
genome里，不知什么原因，位点很少，几个constructs的最后一步，三片断连接都是两
边用Sal I和中间另一个酶。有一个cloning strategy, 整整两天都没有想出来，只好
周末放弃了，去钓鱼，突然想出来的。那时候NEB catalog是比Molecular Cloning还... 阅读全帖 |
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m*********D
发帖数: 1727 | 10 哈哈，是啊，sequencing gel跑了多年，压那些X-光片，都是美好的回忆。
读博士的时候，在国内给Bio-rad纯化一种高温DNA polymerase，能解决sequecing反应
时不少二级结构的问题，还挣过几千外快呢。S-35是后来的吧，记得前面用p-32，后来
是P-33，再到S-35。
NEB的catalog确实是一本分子克隆的好书。在国内虽然是作分子生物学出生，克隆没少
作，但来美国作博士后，要作knockout construct时，才真正变成cloning expert：
pUC18里要塞进一个20kb的片断，找合适的restriction enzyme都困难呀。记得当时就
一个Not I是识别八个bp的酶，很有用，另一个是Sal I。Sal I是识别六个bp,但在老鼠
genome里，不知什么原因，位点很少，几个constructs的最后一步，三片断连接都是两
边用Sal I和中间另一个酶。有一个cloning strategy, 整整两天都没有想出来，只好
周末放弃了，去钓鱼，突然想出来的。那时候NEB catalog是比Molecular Cloning还... 阅读全帖 |
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t******q
发帖数: 117 | 11 who want to follow up.
Find a optimal way to detect the I and P frame using any kind of algorithm.
any idea? |
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t******q
发帖数: 117 | 12 I frame and P frame are the baseline frame type in most of the video
compression scheme.
choose a I and P frame properly will improve the encoding speed, reduce the
bit rate, enhance the SNR.
To detect the significant secence change in the frames is the key
feature in I/P predication.
some just use a pattern like IppppIpppp................
that is not effiecent if the secene changes less, most of the headshoulder
serial is this case.
what feature is used as the key feature?
how much change of th |
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