d******s
发帖数: 674 | 1 First of all, wrong approach: bare silica column in HILIC mode uses silanol
group for retention, which is also the main cause of peak tailing. Adding
TFA which is the common practise in RPC to suppress silanol interference
will not work here.
Why use this column in HILIC mode? Please don't say that you only have this
column.
Reverse phase for peptide/protein digest is the golden standard, and you
will have much better luck to start here. Or, if you are trying to do something
different, you can t... 阅读全帖 |
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v******0
发帖数: 265 | 2 Ok, but the peaks I got from bare silica on small molecules are also ugly.
But the molecules I tested until now are something people use 80%ACN as
isocratic to elute, such as uracil, cytosine, etc.
Is that because these are not retained well with my column, so I don't have
good peaks. If I goes for some other more hydrophilic small molecules, could
it be better?
The other question is since Silanol makes the peptide peak tailing, if I use
UV as detector, which doesn't require acid, could the resu... 阅读全帖 |
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m*****e
发帖数: 1506 | 4 Synthesis and properties of waterborne self-crosslinkable sulfo-urethane
silanol dispersions
Journal of Polymer Science Part A: Polymer Chemistry
Volume 40, Issue 17, pages 3037–3045, 1 September 2002
please send to j*[email protected]. Thanks a lot |
|
m**n
发帖数: 206 | 5 加酸在溶剂中试试，应该可以减少silanol的吸附，比如0.1% tfa or 0.5%formic acid
gradient |
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v******0
发帖数: 265 | 6 Thanks a lot.
Currently, I am only having this bare silica HILIC column, and the reason I
am still doing that is because I need test whether they are working properly
. My purpose is not to find a way separating some peptides, since I already
got good one with C18 column.
I might get some Amide 80 one, but it will take a while. I have little
experience on HILIC, so based on your opinion, I won't have good results (I
mean nice peak) using bare silica, no matter how I optimize the condition,
right... 阅读全帖 |
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v******0
发帖数: 265 | 7 If I am not using peptide standards, do you know whether there are some
other standards could give nice peak in bare Silica HILIC using nanoflow?
silanol
this
something
much easier |
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d********y
发帖数: 176 | 8 http://onlinelibrary.wiley.com/doi/10.1002/masy.19900320118/pdf
On the nature of active species in cationic ring-opening polymerization of
cyclodimethylsiloxanes
Authors
Pierre Sigwalt,
Vivian Stannett
First published: February 1990Full publication history
DOI: 10.1002/masy.19900320118View/save citation
Cited by: 5 articlesCitation tools
Abstract
Contrarily to cationic ring-opening polymerization of cyclic ethers and of
some other cyclic monomers, for which direct identification of the various
t... 阅读全帖 |
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d********y
发帖数: 176 | 9 http://onlinelibrary.wiley.com/doi/10.1002/masy.19910420119/pdf
Article
Inhibiting or cocatalytic effect of water and other additives on cationic
polymerization of cyclodimethylsiloxanes
Authors
P. Sigwalt,
C. Gobin,
P. Nicol,
M. Moreau,
M. Masure
First published: March 1991Full publication history
DOI: 10.1002/masy.19910420119View/save citation
Cited by: 14 articlesCitation tools
Funding Information
Abstract
Cyclodimethylsiloxanes such as hexamethylcyclotrisiloxane (D3) and
octamethyl-cyclotetr... 阅读全帖 |
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l*****g
发帖数: 556 | 10 add ethanol to help solution to spread/evaporate. And modify the glass
surface to increase silanol density if necessary. Or even silane treat the
glass. |
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m*****e
发帖数: 1506 | 11 【 以下文字转载自 Chemistry 讨论区 】
发信人: matrice (matrice), 信区: Chemistry
标 题: reference help, Thanks a lot
发信站: BBS 未名空间站 (Fri Jan 21 22:03:30 2011, 美东)
Synthesis and properties of waterborne self-crosslinkable sulfo-urethane
silanol dispersions
Journal of Polymer Science Part A: Polymer Chemistry
Volume 40, Issue 17, pages 3037–3045, 1 September 2002
please send to j*[email protected]. Thanks a lot |
|
