a****o
发帖数: 2 | 1 忘了补充两点
the PCR products for making qPCR standard also had smeary bands. I had tried
my best to minimized it by nested PCR with low concentration of DNA
template. But I assume certain unspecific amplifications still remained in
the qPCR standard, which could lead to smeary bands for the samples of qPCR
standard. It however does not explain the existence of smeary bands for the
tested samples.
Another thing it the Ct value is smaller than usual. |
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l******r
发帖数: 544 | 2 http://www.kenrockwell.com/canon/5d-mk-iii.htm
As expected, stupid-high ISOs like ISO 25,600 work, but look crummy. As expected, they're soft and smeary. They look exactly like ISO 3,200 from point-and-shoots: usable, but soft and smeared in firmware to hide the noise. In other words, with the right post-processing software, you'll get the same results and can get the same stupid-high ISOs from the older cameras by push-processing.
Real photographers don't need ISO 6,400, much less ISO 12,800. P... 阅读全帖 |
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x5
发帖数: 27871 | 3 这个又是个不靠谱的，5DIII的AF和7D的差的大了去了
expected, they're soft and smeary. They look exactly like ISO 3,200 from
point-and-shoots: usable, but soft and smeared in firmware to hide the noise
. In other words, with the right post-processing software, you'll get the
same results and can get the same stupid-high ISOs from the older cameras by
push-processing.
means drawing with light, not darkness.
anyway.
! The 50/1.8 is a much faster lens than the 24-70/2.8, and sharper, too —
for just $120. f/2.8 is slow, ... 阅读全帖 |
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n**b
发帖数: 13203 | 4 黑的我都看不下去了。。。
expected, they're soft and smeary. They look exactly like ISO 3,200 from
point-and-shoots: usable, but soft and smeared in firmware to hide the noise
. In other words, with the right post-processing software, you'll get the
same results and can get the same stupid-high ISOs from the older cameras by
push-processing.
means drawing with light, not darkness.
anyway.
! The 50/1.8 is a much faster lens than the 24-70/2.8, and sharper, too —
for just $120. f/2.8 is slow, for daylight use... 阅读全帖 |
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a****o
发帖数: 2 | 5 各位大侠帮帮忙。
我们小组是做土壤微生物的。最近我们组的qPCR 和PCR 的质量都不理想，两个assays
跑出来的gel都很模糊。Both assays give smeary bands in agarose gel, and it
sometimes happens to have double bands for qPCR.
This problem used to occurred quite a long time ago. And some of the assays
are quite routine in the lab. Our group discuss this problem before, and
cannot solve it. Anyone can give recommendations? Many thanks.
Very urgent. |
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