q**s
发帖数: 285 | 1 try add 2-mercaptoethanol in medium if you didnot add
The role of 2-mercaptoethanol in the stimulation of spleen cell cultures:
increased uptake of cystine into the TCA-soluble pool
Immunol Lett. 1982 Apr;4(4):193-7. |
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D**p
发帖数: 185 | 2 之前在NIBS见过他，说过一句"we believe everything soluble can be crystallized
"，没想到经历这么传奇。还是很牛了，尽管是奴隶主吧，但一般人，包括班上绝大部
分人，估计也就在造纸厂呆一辈子了。优点和毅力还是很值得佩服的。 |
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f***x
发帖数: 1524 | 3 hybridomas筛的是🐭源抗体，需要人源化。前期需要免疫动物。immune
response 是个问题。优点是有in Vivo quality control,一般不再需要affinity
Maturation and solubility/stability optimization.
相比较，display 可以从人工库筛，库可以很大(10e7-10e11)，用时短，不用考虑免疫
问题。但是往往需要affinity maturation.
Phage display 筛fragment.
yeast display可以 筛full length antibody.
方法上，phage display 简单。主要是Elisa and affinity binding/washing.
yeast display 用到FACS,可以对筛选有很好的控制，同时筛Antibody expression/
stability 和antibody-antigen binding. |
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z*t
发帖数: 863 | 4 受教了！您怎么看alpaca里搞得nanobody?
：hybridomas筛的是🐭源抗体，需要人源化。前期需要免疫动物。immune
：response 是个问题。优点是有in Vivo quality control,一般不再需要affinity
：Maturation and solubility/stability optimization.
：相比较，display 可以从人工库筛，库可以很大(10e7-10e11)，用时短，不用考虑免
疫问题。但是往往需要affinity maturation.
：Phage display 筛fragment.
：yeast display可以 筛full length antibody.
：方法上，phage display 简单。主要是Elisa and affinity binding/washing.
：yeast display 用到FACS,可以对筛选有很好的控制，同时筛Antibody expression/
：stability 和antibody-antigen binding. |
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w***t
发帖数: 86 | 5 > 1）Chapoy, A.; Mokraoui, S.; Valtz, A.; Richon, D.; Mohammadi, A.
> H.; Tohidi, B. Solubility measurement and modeling for the system
> Propane-Water from 277.62 to 368.16 K. Fluid Phase Equilib. 2004,
226,213
>
>
> 2）Phase and critical behavior in type III phase diagrams
> Author: Quinones-Cisneros S.E.1
> Source: Fluid Phase Equilibria, Volume 134, Number 1, June 1997 , pp.
> 103-112(10)
> Publisher: Elsevier
>
学校图书馆放假，online的又没有，有点急着用，谢谢！附件请发至w***[email protected].
cn |
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w***t
发帖数: 86 | 6 1）Chapoy, A.; Mokraoui, S.; Valtz, A.; Richon, D.; Mohammadi, A.
> H.; Tohidi, B. Solubility measurement and modeling for the system
> Propane-Water from 277.62 to 368.16 K. Fluid Phase Equilib. 2004,
226,213
>
>
> 2）Phase and critical behavior in type III phase diagrams
> Author: Quinones-Cisneros S.E.1
> Source: Fluid Phase Equilibria, Volume 134, Number 1, June 1997 , pp.
> 103-112(10)
> Publisher: Elsevier
学校放假图书馆不开，online version 没有，急用， 谢谢！ 请发至w***[email protected]
.cn
> |
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c******e
发帖数: 737 | 7 实验想配Methane water solution, 急需看看这篇paper, 可学校没有。
哪位能够上ScienceDirect的可以帮忙down一下，send到：
j********[email protected]
谢谢
Gas solubility measurement and modeling for methane–water and methane–
ethane–n-butane–water systems at low temperature conditions
Fluid Phase Equilibria, Volume 220, Issue 1, 15 June 2004, Pages 111-119
Antonin Chapoy, Amir H. Mohammadi, Dominique Richon and Bahman Tohidi |
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b*****e
发帖数: 585 | 8 那个组是做这些的，帮忙看看有没有前途，thx～
Membrane Separation Process, Gas Solubility
Estimation, Ion Permeation Process, and Biological Separation Process
characterized by NMR |
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j***i
发帖数: 4975 | 9 单凭感觉
这东西应该是不溶于水的
不过长期和水放在一起
可能能搞出个水合物结晶水CU（OH）2什么的吧
GOOGLE一下好了
找SOLUBILITY或者是MSDS |
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c********r
发帖数: 2227 | 11 SOLUBLE POLY(ETHER KETONE)S AND POLY(ETHER SULFONE)S FROM PHENYL-SUBSTITUTED
HYDROQUINONES
JOURNAL OF MACROMOLECULAR SCIENCE-PURE AND APPLIED CHEMISTRY
Volume: A29 Issue: 12 Pages: 1141-1154 Published: 1992
我这里link 总是不能链接，麻烦哪位xdjm帮个忙
包子答谢 |
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w***u
发帖数: 116 | 12 PEGSH is soluble in toluene, no problem |
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d****i
发帖数: 360 | 13 I tried to dissolve this N-hydroxysuccinimide in DCM. It seems they are not
happy with each other. I always got solids in the liquid. Thanks! |
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n*****7
发帖数: 19 | 15 琥珀酰胺一般都是用DCM溶解，可能你浓度太大，或者没有干燥 |
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n*****7
发帖数: 19 | 16 琥珀酰胺一般都是用DCM溶解，可能你浓度太大，或者没有干燥 |
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w******n
发帖数: 13202 | 17 是不太好溶，我一般做它的活化脂的时候，都用DMF |
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B******1
发帖数: 9094 | 18 Another way is to add acetyl chloride slowly to a stirred, cold methanol
solution. You can calculate the amount of acetyl chloride needed before hand.
But I don't know the solubility of HCl in MeOH. |
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h******g
发帖数: 600 | 19 my product is not soluble in water.
lots
the |
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B******1
发帖数: 9094 | 20 MeOH and Pd/c is a dangerous combination. Better avoid that combo all
together. Switch the solvent to EtOH would reduce the "fire/combustion"
risk dramatically. When doing filtration to remove the used catalyst, make
sure you do NOT make the catalyst super dry and exposed to air.
I agree EtOAc is a much benign solvent for hydrogenation in this respect.
But the speed of hydrogenation therein is very slow compared to using EtOH.
For solubility concerns, a mixture of EtOH/EtOAc seems to work fo |
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d********O
发帖数: 1196 | 21 Title:Soluble High-Temperature Polyterephthalamides
M
acromolecules, 1998, 31 (1), pp 208–209
DOI: 10.1021/ma971194e
Please send to D********[email protected], if this is more convenient for you.
Thanks again! |
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d*******7
发帖数: 188 | 22 Title : Measurement of permeability, diffusion and solubility
coefficients. A testing method
Author: C.Rodrigo Correaa and A. Kleina
Journal: Polymer Testing Volume 9, Issue 4, 1990, Pages 271-277
thanks for the help!
l***********[email protected] |
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c******h
发帖数: 13 | 23 Chemistry - A European Journal
Volume 9 Issue 6, Pages 1416 - 1425
Published Online: 11 Mar 2003
Homogeneous Catalysts Supported on Soluble Polymers: Biphasic Sonogashira
Coupling of Aryl Halides and Acetylenes Using MeOPEG-Bound Phosphine-
Palladium Catalysts for Efficient Catalyst Recycling
Axel Köllhofer, Dipl.-Chem., Herbert Plenio, Prof. Dr *
thanks |
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y*****6
发帖数: 47 | 31 求博士论文下载，多谢多谢
title： The influence of feeding diets containing corn dried distillers
grains with
solubles (DDGS) to grower-finisher pigs on growth performance, carcass and
pork
quality
author: Guowu Xu, from university of minnesota.
请发至信箱：j*****[email protected]
多谢。 |
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b**********e
发帖数: 531 | 33 有谁用过zonyl BA 7-8 个CF2末端一个乙醇，这个东西除了MEK Acetone 醇， 还有什
么熔剂能容？acetonitrile 能容马？
试过DMF, DMAc, THF 都不容 |
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l********e
发帖数: 41 | 34 Can anyone be so nice to provide the electronic copy of the following books?
Thank you very much!!!
Book 1:
Supercritical Carbon Dioxide: in Polymer Reaction Engineering
Publisher: Wiley-VCH Verlag GmbH & Co. KGaA
Editor(s): Maartje F. Kemmere, Thierry Meyer
Print ISBN: 9783527310920
Online ISBN: 9783527606726
Book 2:
Natural Extracts Using Supercritical Carbon Dioxide [Hardcover]
Edited by Mamata Mukhopadhyay
Publisher: CRC press
Book 3:
Solubility in Supercritical Carbon Dioxide
Edited by: Ra |
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b*******r
发帖数: 1073 | 36
One can "tune" the paramagnetic effects on T1 by changing the paramagnetic
agents (some agents give stronger transverse PRE while some others give
smaller T1 PRE but stronger other effects). Which agent to use I guess
depends on your goals and other practical considerations like availability,
solubility, toxicity, or whatever.
1. Repeatable sample preparation
(also, increase the difference in spin concentrations to magnify the effect
?)
2. NMR measurement
(D1 should be long enough, delays s |
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o******r
发帖数: 57 | 37 The Chemistry of Silica: Solubility, Polymerization, Colloid and Surface
Properties and Biochemistry of Silica by Ralph K. Iler
MANY THANKS! |
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s****2
发帖数: 4569 | 38 if it forms TEA salt, say orotic acid.2(3)TEA salts, it readily soluble in
DMF/ACN, even DCM. |
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s*******e
发帖数: 5 | 40 Title: Reinforced Films Based on Cross-Linked Water-Soluble Sulfonated
Carbon Nanotubes with Sulfonated Polystyrene
Author(s): Ying, D; Hong, HP; Guiver, M, et al.
Source: JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY Volume: 9 Issue: 9
Pages: 5150-5156 Published: 2009
pls sent to : s***********[email protected]
Thanks in advance! |
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s**********r
发帖数: 67 | 41 1. Girardot, C., G. Lemercier, et al. (2007). "Novel ruthenium(II) and zinc(
II) complexes for two-photon absorption related applications." Dalton
Transactions(31): 3421-3426.
2. Lemercier, G., C. Martineau, et al. (2006). "Analogs of Michler's ketone
for two-photon absorption initiation of polymerization in the near infrared:
synthesis and photophysical properties." New Journal of Chemistry 30(11):
1606-1613.
3. Hayek, A., F. Bolze, et al. (2006). "Synthesis and characterization of
water-solubl... 阅读全帖 |
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d****i
发帖数: 360 | 42 1. Enzymatic cleavage of side chains of synthetic water-soluble polymers
(pages 2833–2848)
J. Drobník, J. Kopeček, J. Labský, P. Rejmanová, J. Exner, V.
Saudek and J. Kálal
Macromolecular Chemistry and Physics
Thanks a lot! |
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d****i
发帖数: 360 | 43 Enzymatic cleavage of side chains of synthetic water-soluble polymers
(pages 2833–2848) 1976
J. Drobník, J. Kopeček, J. Labský, P. Rejmanová, J. Exner, V.
Saudek and J. Kálal
Macromolecular Chemistry and Physics
Thanks a lot! |
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x*******i
发帖数: 32 | 44 Hello There,
We have a protein (pI = 8.9, 10 mg/mL, MW 100 K) precipitated in 10 mM
phosphate buffer (pH around 7) after overnight storage at 4 degree. The
precipitate will be soluble again when warming up solution to room
temperature. Addition of NaCl will prevent precipitation. Other common
buffers are OK not causing precipitate.
Hypothesis is ionic cross-linking. Phosphate ion has intense negative charge
density and working as the cross-linking agent, its multiple negative
charges interact wi... 阅读全帖 |
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b*******g
发帖数: 1309 | 45 not soluble in in ACN?
negtive ion mode generally is not sensitive, and you can detect phosphorylated peptides in positive mode. |
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d****i
发帖数: 360 | 46 Journal of Materials Science: Materials in Medicine
Volume 22, Number 3, 469-479, DOI: 10.1007/s10856-011-4243-5
Synthesis and characterization of ionic charged water soluble arginine-based
poly(ester amide)
Jun Wu, Martha A. Mutschler and Chih-Chang Chu
Thanks a lot! |
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k****o
发帖数: 728 | 47 问个溶解性的问题，一个反应要把O-phosphorylethanolamine跟一个几千Da的
biomacromolecule连起来，要在organic solvent里进行。但查了半天O-
phosphorylethanolamine只溶于水，而micro soluble 于methanol和ethanol（算下来
最多几个mM）。有什么办法解决这个问题吗? 至少要能达到50 mM。 |
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J**2
发帖数: 30 | 48 回国以后的经历很牛，不过文章一般，应该很能忽悠
葛子义，博士，研究员，博士生导师。
1999年毕业于兰州大学，获有机化学专业学士学位；
2004年毕业于中科院化学所，获理学博士学位；
2004-2005年，中科院化学所任助理研究员，主要从事高温高分子材料研究；
2005年-2007年，日本东京工业大学博士研究员，主要从事有机发光材料（
OLED）与器件研究；
2007年-2008年，日本神奈川大学博士后，主要从事导电高分子可控合成研究；
2008年-2009年，澳大利亚新南维尔市大学博士后，主要从事柔性显示技术和
有机太阳电池研究；
2009年3月，任华东理工大学化工学院教授，主要从事有机光电材料研究；
2010年4月以“团队行动”引进，任新能源所研究员、博士生导师。
目前主要从事有机太阳能电池、有机发光材料（OLED）和导电功能高分子合成
方面的研究，在Advanced Functional Materials, Chemistry of M... 阅读全帖 |
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d***e
发帖数: 1215 | 49 很多时候并不一定需要太多的solubility，只要有一小点点就足够做分析了。 |
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m**n
发帖数: 206 | 50 peptides sure will have different solubility in different solvent. If you
prepare sample in ACN, diluted sample (10time diluted than RPLC) and
increasing injection volume (10times injection of RPLC) will probably make
those peaks visible. |
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