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w*******y
发帖数: 60932 | 2 Palit NE5X460SF1102 GeForce GTX 460 (Fermi) Sonic 1GB 256-bit GDDR5 PCI
Express 2.0 x16 HDCP Ready SLI Support Video Card w/ Tom Clancy's HAWX 2
for PC + G.SKILL Ripjaws Series 8GB (2 x 4GB) 240-Pin DDR3 SDRAM DDR3 1600 (
PC3 12800) Desktop Memory Model F:
http://www.newegg.com/Product/ComboDealDetails.aspx?ItemList=Combo.559989
Price break down as follows:
Palit Microsystems NE5X460SF1102: $189.99
2. G.SKILL F3-12800CL9D-8GBRL: $99.99
Combo Discount: -$35.00
Combo Price: $254.98
$35.00 Mail-... 阅读全帖 |
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w*******y
发帖数: 60932 | 7 Delta Sonic
FREE Super Kiss Car Wash - Worth 12 $$
FREE 10-Minute Interior Cleaning - worth $$ 8
If you sign up for their email (First time people only, they print the car
License Plate # on the coupon to be printed and coupon is valid for 2 weeks)
Link:
http://register.deltasoniccarwash.com/emailclub/
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w*******y
发帖数: 60932 | 8 Found the oral-B Sonic Complete set at Kmart today. Original price 126.00
now 32.00. These are going for around 100.00 at amazon.com Number on the
receipt is 06905583295
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w*******y
发帖数: 60932 | 9 Microcenter has the Palit NE5X460SF1102 GeForce GTX 460 Sonic 1024B GDDR5
PCIe 2.0 x16 Video Card for 59.99 AR + Tax.
Link:
http://www.microcenter.com/single_product_results.phtml?product
This card has mixed reviews due to fan noise and lower quality heat sync
than the Platinum edition. However, this is a great deal for the price.
You can actually put an 8800 cooler on it for better cooling performance.
Low stock item at my Dallas store so act fast!
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w*******y
发帖数: 60932 | 18 Newegg
Sonic GenerationsXbox 360
PS3
= $20 with free shipping
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g******y
发帖数: 2517 | 19 Oral B Pulsonic Sonic Electric Rechargeable Power Toothbrush, 29% Off+$20
Rebate, 原价$70, 现价$30
http://amzn.to/ypFn8M |
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w*******y
发帖数: 60932 | 22 Starts 05/26/12 @ AmazonMass Effect 3:
http://iway.org/9627321
- $29.99
Mass Effect 3 Digital Deluxe Version - $39.99
Syndicate - $17.99
Tera Online Collector's Edition - $29.99
Tera Online - $24.99
FINAL FANTASY XIV - $7.99
Final Fantasy XI: Ultimate Collection Abyssea Edition - $7.99
Square Enix Ultimate Collection - $7.49
Hitman Triple Threat Pack - $6.99
Just Cause - $7.49
The Last Remnant - $14.99
Order of War - $4.99
Scarygirl - $4.99
Battlestations Pacific - $4.99
Battlestations Midway ... 阅读全帖 |
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g*********5
发帖数: 2533 | 23 Isolation of nuclei for the co-IP and whole ChIP protocols is based on the
methods of Gendrel et al. (2002, 2005), Johnson et al. (2002), and Nelson et
al. (2006).
METHOD
For extraction and co-IP of nuclear proteins, see Steps 1-39 (Fig. 1). For
the ChIP procedure, see Steps 40-69 (Fig. 2).
Figure 1.
View larger version:
In this page
In a new window
Figure 1.
Flowchart for the timeline and organization for co-IP of nuclear proteins
from Arabidopsis seedlings.
Figure 2.
View larger versio... 阅读全帖 |
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t*******h
发帖数: 2744 | 24 Beatdom
ABOUTSUBMISSION GUIDELINESBUY
Home/Beatdom Content/Noise Poetry: An Interview with Thurston Moore
Thurston Moore reading his poetry at Naropa University's Summer Writing
Program, 2011.
Noise Poetry: An Interview with Thurston Moore
Share
0
0
0
As an MFA fiction student at the Jack Kerouac School of Disembodied Poetics
at Naropa University (one of the longest, most consistently-made-fun-of
school names ever), I was fortunate enough to be brought face-to-face with
some of the most legendar... 阅读全帖 |
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h***9
发帖数: 8948 | 25 Buy 1 Get 1 free on used games $14.99 and under will be starting up again on
Tuesday, 12/7!
Below is a list of $14.99 and under games at Blockbuster...
$6.99 or less
Arcade Unplugged
Armored Core 4
Beautiful Katamari
Blue Dragon
Bullet Witch
Club, The
Condemned 2
Dark Messiah of Might and Magic
Dark Sector
Dark Void
Dead Or Alive 4
Dirt ($4.99)
Earth Defense Force
Fantastic 4 ($4.99)
Frontlines Fuel of War
Golden Compass ($4.99)
Guitar Hero III
Guitar Hero Aerosmith
Guitar Hero World Tour
Harr... 阅读全帖 |
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Z*****o
发帖数: 4090 | 27 http://www.stereophile.com/features/368/index.html
By Robert Harley • Posted: Nov 1, 1993
Not that long ago, digital audio was considered perfect if all the bits
could be stored and retrieved without data errors. If the data coming off
the disc were the same as what went on the disc, how could there be a sound-
quality difference with the same digital/analog converter? This "bits is
bits" mentality scoffs at sonic differences between CD transports, digital
interfaces, and CD tweaks. Becaus... 阅读全帖 |
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l*******g
发帖数: 28502 | 28 ☆─────────────────────────────────────☆
ppang (胖胖) 于 (Mon Nov 1 11:48:37 2004) 提到:
很多不了解农村实际情况的发言，大家有时间不妨去发出自己的声音。
☆─────────────────────────────────────☆
ooo (KKK) 于 (Mon Nov 1 15:13:43 2004) 提到:
好，这就去看看
☆─────────────────────────────────────☆
oestrus () 于 (Mon Nov 1 22:40:03 2004) 提到:
那倒是麻烦ooo同学去chinanews 给介绍一下农村的各种负担。比如以你家为例（假设你
是从农村来），最好是一笔一笔有数字的。省的我们不了解情况的以后瞎扯。
谢谢了。嗯，对了，要是觉得有攻击的意思，那肯定不是本意。原谅先。
☆─────────────────────────────────────☆
ooo (KKK) 于 (Tue Nov 2 12:48:12 20... 阅读全帖 |
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g********d
发帖数: 19244 | 29 ☆─────────────────────────────────────☆
lxy52136 (sunny) 于 (Sun Nov 3 14:24:24 2013, 美东) 提到:
11月4日update:出事地点的卫星地图贴在了84楼
出事地点在INTERSTATE95 52至51这段路上（车已过52号出口准备从51下）
我家买的保险是全保，自己这边修车要掏500元
68楼我放了事故报告单，第4页67楼是我马赛克我家车牌后的3张事故后的照片
对方被撞情况，我问了一下老公，前边撞坏，侧身没看
老公前天晚上7：30左右自己一人驾车从高速开车回家，离出口还有1迈左右的距离后，
换lane到最右侧车道，当继续行使半迈左右后和一车发生碰撞，当时老公觉得车内有很
强的压迫感，自己有清醒的意识大概在出事5、6秒后，发现一车飞快的从他右侧飞奔儿
去，我老公觉得他车速度之快，可能要逃跑，下意识的开车去追，但那车在前方不远处
的紧急停车带上停下了，我老公把车停在他后面。这人下车后十分惊慌，在等警察的时
候问我老公是不... 阅读全帖 |
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c******r
发帖数: 3778 | 30 如果只是为了跑western，sonicate足够了。
你load了多少protein？sonicate多大的volume？在1.5ml管子里还是15ml管子？多长时
间？多少次？
要根据体积选用不同的头。如果体积太大，sonicate效果不好。如果体积太小，
sonicate很容易起泡泡。一旦起泡泡sonicate就没用了。
一般sonicate之后弹一下管子，以不粘稠可以自由流动为准。否则就再sonicate 20秒。
之后像ls说的，离心max speed 5min，取上清，95度10分钟，冷却，上样。
是很
protein |
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w*******y
发帖数: 60932 | 31 There is more to this and lasts to near end of the month..Thought people
would like to know this slick deal before it hits stores..
**Some deals are only for a LIMITED TIME (1 week only) and will not be on
sale for the whole duration. Which leads me to believe they could add more
games throughout the sale**
The Power Save Sale starts this Friday April 1, 2011 and ends at the close
of business on Sunday April 24, 2011
SPECIAL PROMOTIONS
Free Little Big Planet (PSP) with a purchase of a PSP 30... 阅读全帖 |
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l*******g
发帖数: 28502 | 32 ☆─────────────────────────────────────☆
sonic (sonic) 于 (Thu Feb 22 14:52:22 2007) 提到:
我小时候上学，要穿过一大片农田走着到另外一个单位的子弟小学。路上还经过三条小
河，夏天中午就跳进河中捉鱼摸螃蟹，玩得很是快活。秋高气爽的时候，我找一个小山
包，放一把火全烧光，躲在一旁看，那高兴劲，就别提了。春天山包上有不少草莓可摘
，走过稻田随手扯下几根嫩稻杆，一片清香。儿子现在整天就是电视与一堆玩具，我觉
得没劲得很。
十年前和少时伙伴回去过一次。站在一高处眺望四周，山川河流农田都仍是旧时风景，
就是农民的房子从茅草屋变成了二层或三层砖房。四下里有不少围墙围起来的效区单位
，这冰箱厂那电表厂，这边林业学校那边电力大学，散布其间，到是有些新房子，也不
太显眼。
这次回去，听说当地变成了一个庞大的开发区，完全变了样，什么大学城，又是什么新
农村，光听形容也想不明白。和几个春节回来的少时伙伴一合计，都很有兴趣。带上老
婆儿子，叫了出租车就奔了过去。
估摸着开到了地，就开始迷糊了。记得清清楚楚的小时候的山山水... 阅读全帖 |
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a******s
发帖数: 4052 | 33 from wiki:
DVD-Audio authoring software
Normal DVD(Video) authoring software usually does not support DVD-Audio
creation, so there is some special software:
Macintosh
Sonic Solutions DVD Creator AV – The first DVD-Audio authoring solution
available. A spin off of the popular high end DVD Video authoring package.
It allows DVD-Audio authoring at the command line level only. Still widely
used but no longer sold or supported by Sonic Solutions.
Sonic Studio SonicStudio HD – Macintosh based tool use... 阅读全帖 |
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w**z
发帖数: 8232 | 34 http://www.linkedin.com/today/post/article/20131217070749-52594
IVO, a programmer: Ivo Sega here.
CAROL, a company recruiter: Hi Ivo! It's Carol from Vector Industries.
Thanks again for coming out to meet everyone last week. Josh, our CTO, was
really happy to meet you.
IVO: No problem. I was happy to meet him too. You've got some interesting
projects going on. It could be a lot of fun to tackle one of them.
CAROL: I'm glad to hear it. Can you come back and meet more of our team next
Tuesday at t... 阅读全帖 |
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c****7
发帖数: 125 | 35 大家都入了什么颜色的？
发现颜色不同功用描述上有些差异。
比如
The Clarisonic Mia Sonic Cleansing System – Pink is a travel friendly
device that comes with an international charger for easy use when away from
home. Great skin can now be accomplished every day while on the go. The
Clarisonic Mia Sonic Cleansing System – Pink works great at the sink or on
the counter. Use it with your favorite cleanser for great results. With
regular use of the The Clarisonic Mia Sonic Cleansing System – Pink, the
skin will see cumulative resul... 阅读全帖 |
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C******g
发帖数: 2930 | 36 Sales
* Sonic & Sega All Stars Racing Add On Bundle – Sale (now $6.49,
original price $12.99)
* Sonic & Sega All-Stars Racing DLC 2 – Metal Sonic & Death Egg Zone
Track – Sale (now $5.49, original price $7.99)
* Sonic & SEGA All-Stars Racing – Ryo with Forklift Character – Sale (
now $3.49, original price $4.99)
* Sonic The Hedgehog Very Hard Shadow – Sale (now $1.49, original price
$2.49)
* Sonic The Hedgehog Very Hard Silver – Sale (now $1.49, original price
$2.49)
* So |
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g********d
发帖数: 19244 | 37 ☆─────────────────────────────────────☆
USAst1 (千寻) 于 提到:
今天要把新买的一辆二手车保险给买了，人生的第一辆车啊！
网上quote了geico，state farm，跟agent要了liberty mutual的，最后一个最便宜，
选的都是最低保额的，collision和comprehensive的自付都是1000.
但是听说至少liability要选100k的，对于新手要也做全保。
大家觉得呢，全保都包括什么，我很想买的liberty mutual，它给我的报价包括
liability, uninsured motorist coverage, other than collision, collision,
towing and labor, waiver of collision.这是不是全保？
谢谢先！！
☆─────────────────────────────────────☆
time5 (time5) 于 (Thu Oct 31 14:22:43 2013, 美东) 提到:
可以考虑：
B... 阅读全帖 |
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f******u
发帖数: 250 | 38 Just 提醒一下老哥:
Rovi Corporation said today it has agreed to acquire Sonic Solutions in a
cash and stock deal with an enterprise value of about $720 million. Both
companies provide technology for the digital video and entertainment sector.
The deal values Sonic at $14.17 per share, a 38% premium over its 30-day
average closing price as of Tuesday. Rovi, which provides next-generation
guidance solutions including TotalGuide, discovery, metadata advertising and
networking technologies, said Sonics di... 阅读全帖 |
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g****r
发帖数: 88 | 39 用Cup-horn sonicator, I mean placing the microcentrifuge tube containing
the DNA sample into an ice-water bath in the cup-horn sonicator.
这样，超声波直接作用的是tube外的冰水，间接sonicate your sample DNA.You only
need order a Cup-horn which matches your sonicator.用的时候，将microprobe
旋下， 将Cup装上，倒入冰水，就成了。用这个，做小体积的sonication效果非常好。 |
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c********b
发帖数: 363 | 40 每个步骤都是别人都知道的,我只是组装了一下. 最大不同是别人是先Urea变性,提出来
再复性.我是利用Triton X-100可以洗脱SDS的特点利用SDS变性,然后Triton X100洗脱
复性,然后过柱. 肯定不会对所有蛋白适用,但是起码我遇到的都work (包括一个>100
Kd的酶).
我是用的sonication water bath, 也可以用probe sonication再加SDS,都OK,但是注意
dilution的比例和体积.
Good luck. 用的高兴分我几个包子.
Key, keep a fraction from every step for analyses and trouble shooting
- Culture the E. coli overnight
- Induce it at a proper concentration of IPTG, room temperature for ~
4hrs
- Pellet the E. coli at 6000 rpm at 4 degree
- R... 阅读全帖 |
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l****r
发帖数: 21884 | 41 关于这次音乐节的通稿, 提到专辑10月初发行, 9月底10月初差不多啦
http://ent.qq.com/a/20110815/000149.htm
近日，华谊兄弟音乐公司旗下当红歌手，实力派唱作人陈楚生(微博)应邀前往日本，在
亚洲最大音乐节之一“Summer sonic”舞台上，激情献唱一曲其原创作品《奇幻之旅》
，这也是国内音乐人首度受邀登陆该音乐节。陈楚生以其摇滚霸气的台风，扎实卓越的
唱功，几度引爆歌迷情绪，现场气氛一度High到顶点。
顶级音乐节聚世界当红歌手 陈楚生认真排练不敢怠慢
“Summer sonic”始办于2000年，是亚洲最大音乐节之一，也是日本目前最为当红的音
乐节。每年献唱的艺人都在国际上享有盛誉。往年登台的不但有英伦大牌coldplay、朋
克乐队Sex Pistols、电音鬼才The prodigy、殿堂级迷幻乐团the verve、也有嘻哈音
乐教皇Jay Z、格莱美乡村民谣歌手Taylor Swift，美国教父级创作歌手Stevie Wonder
以及嘻哈组合A Tribe Called Quest等。今年也是“Summer sonic”首度引进... 阅读全帖 |
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w*******y
发帖数: 60932 | 42 This is my first deal posting, so please be gentle...
To go along with the Capcom, EA, and Gameloft app sales going on currently,
Sega also has many of their iPhone & iPad games on sale!
Appstore average ratings are in parentheses. All apps listed are $0.99
except for the Sonic the Hedgehog games as noted below:
Virtua Fighter 2 (3.5)
Altered Beast (4.0)
Sonic Spinball (4.0)
Gunstar Heroes (3.5)
ChuChu Rocket (4.5)
ChuChu Rocket HD [iPad] (4.0)
Super Monkey Ball 2 (3.5)
Super Monkey Ball 2: Sak... 阅读全帖 |
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w*******y
发帖数: 60932 | 43 Disney
.99
iPhone
Alice In Wonderland
Disney Fairies Fly
Phineas and Ferb Arcade
Split/Second
TRON: Legacy
Toy Story Mania
Universal
The Incredible Machine
iPad
JellyCar 2 on iPad
Phineas and Ferb Arcade
Split/Second
Disney Fairies Fly
TellTale
.99
iPhone
Monkey Island Tales 1
Puzzle Agent
Puzzle Agent 2
HECTOR: Ep1- We Negotiate with Terrorists
HECTOR: Ep2- Senseless Acts of Justice
HECTOR: Ep3- Beyond Reasonable Doom
2.99
iPad
Sam & Max Episode 1: The Penal Zone for iPad
Puzzle Agent HD
Puzzle... 阅读全帖 |
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w*******y
发帖数: 60932 | 44 New games added since yesterday.
Disney
.99
iPhone
Alice In Wonderland
Disney Fairies Fly
Phineas and Ferb Arcade
Split/Second
TRON: Legacy
Toy Story Mania
Universal
The Incredible Machine
iPad
JellyCar 2 on iPad
Phineas and Ferb Arcade
Split/Second
Disney Fairies Fly
TellTale
.99
iPhone
Monkey Island Tales 1
Puzzle Agent
Puzzle Agent 2
HECTOR: Ep1- We Negotiate with Terrorists
HECTOR: Ep2- Senseless Acts of Justice
HECTOR: Ep3- Beyond Reasonable Doom
2.99
iPad
Sam & Max Episode 1: The Penal Zon... 阅读全帖 |
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w*******y
发帖数: 60932 | 45 Some of these may have already appeared in other posts.
Got my information from here:
http://www.ign.com/wikis/app-store/Holiday_2011_Sale
.
All are on sale for 0.99 (until the end of the list)
2k Games
NBA 2K12 for (iPad) ($4.99)
NBA 2K12 for (iPhone) ($2.99)
NHL 2K11 for (iPad) ($1.99)
NHL 2K11 for (iPhone) ($0.99)
Civilization Revolution for (iPad) ($1.99)
Civilization Revolution for (iPhone) ($0.99)
Sid Meier's Pirates! for (iPad) ($1.99)
Carnival Games ($0.99)
Carnival Games Vol. 2 ($0.99)... 阅读全帖 |
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w*******y
发帖数: 60932 | 46 Link:
http://www.amazon.com/gp/product/B006J97RSO
The SEGA 48 Game Mega Arcade Pack contains:
Golden Axe
Altered Beast
Comix Zone
Ecco the Dolphin
Gain Ground
Shinobi III: Return of the Ninja Master
VectorMan
Crack Down
Shadow Dancer: The Secret of Shinobi
Space Harrier II
Alex Kidd in the Enchanted Castle
Bonanza Bros.
Columns
Ecco Jr.
Eternal Champions
Fatal Labyrinth
Galaxy Force II
Kid Chameleon
Ristar
Super Thunder Blade
Alien Storm
Bio-Hazard Battle
Columns III
Sword of Vermilion
Virtua Fi... 阅读全帖 |
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h****o
发帖数: 2455 | 47 http://en.wikibooks.org/wiki/Acoustics/Sonic_Boom
A sonic boom or "tunnel boom" can also be caused by high-speed trains in
tunnels (e.g. the Japanese Shinkansen). In order to reduce the sonic boom
effect, a special shape of the train car and a widened opening of the tunnel
entrance is necessary. When a high speed train enters a tunnel, the sonic
boom effect occurs at the tunnel exit. In contrast to the (super)sonic boom
of an aircraft, this "tunnel boom" is caused by a rapid change of subsonic
f... 阅读全帖 |
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l*******g
发帖数: 28502 | 48 ☆─────────────────────────────────────☆
sonic (sonic) 于 (Mon Jun 6 21:38:04 2005) 提到:
Admire!
To me those photos are not a surprise as I ever saw 还要贫困一些的 people
in China. The living conditions of 贫困地区 have been improved steadily. But
the gap between rural and urban areas is becoming larger.
people
☆─────────────────────────────────────☆
hguo (Who+am+I?) 于 (Tue Jun 7 12:35:23 2005) 提到:
By searching for "OCEF" on this board, you'll be able to find quite some posts
about Overseas Chi... 阅读全帖 |
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l*******g
发帖数: 28502 | 49 ☆─────────────────────────────────────☆
sonic (sonic) 于 (Mon Feb 12 21:52:24 2007) 提到:
发信人: ardor (HOT), 信区: ChinaNews
标 题: 说说国内农村的变化和进步
发信站: BBS 未名空间站 (Sat Feb 10 17:44:09 2007)
仅就我自己的家乡说说，100%眼见的事实：
1：不缴农业税了。这个农民最有感触，说自从盘古开天地，不缴皇粮第一回。
2：九年义务教育落到实处。其实九年义务教育法早就颁布，在邓江时代就是没有真正
实行，胡把这个做好了。
3：开始实行合作医疗了。当初老邓废除老毛的农村赤脚医生制度，结果搞得农民看不
起病。现在，胡哥在农村医疗改善上有进步。
4：通了自来水。
5：农村修了水泥路。农民自筹50%，政府补贴50%。
6：电网改造，前几年就搞好了，几乎不拉闸停电了。
7：野生动物，如野兔，黄鼠狼，猫头鹰，喜鹊，各种各样的蛇，在村边田舍常见。说
明环境在改善。据说，开一枪，罚1000块。
8：私家小车我们村有好几辆了。
一句话，胡温政府... 阅读全帖 |
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d******g
发帖数: 6966 | 50 http://www.autoblog.com/2011/12/30/2012-
chevy-sonic-brake-pad-recall/
"It turns out that Chevrolet let a few Sonics built between June 2 and
November 21 leave its factory in Orion Township, Michigan minus a brake
pad.
General Motors claims that of the 4,873 Sonics built during that time,
some
20 to 30 may be missing either an inner or outer front brake pad. The
company says 4,296 of the potentially shoe-less Sonics were sent to the
U.S.
, while 577 migrated north to Canadian customers." |
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