o****o
发帖数: 8077 | 1 taking . as 0, this is the SSCP matrix, and can easily be obtained via PROC
CORR
here is an example:
data x;
input x1-x5;
cards;
1 . 1 1 .
. 1 1 1 .
1 1 1 1 1
. . . . .
. 1 . . 1
. 1 . . .
1 . 1 . .
. . 1 . .
. . . 1 .
. . 1 . 1
;
run;
proc corr data=x
out=sscp(where=(_TYPE_="SSCP" & _NAME_^="Intercept") drop=Intercept)
sscp
noprint;
var x1-x5;
run; |
|
e******3
发帖数: 16 | 2 My company is currently seeking a full-time Network Security Specialist to
support one of our government projects.
Must be at least a green card holder
The ideal candidate thrives in a team atmosphere, takes pride in his/her
work, and is committed to a highly proactive, process-driven approach. They
must possess excellent programming skills and be customer oriented. Ideally,
a motivated, self-driven, and results oriented team player with the ability
to learn and adapt quickly to new environments... 阅读全帖 |
|
e******3
发帖数: 16 | 3 My company is currently seeking a full-time Network Security Specialist to
support one of our government projects.
Must be at least a green card holder
The ideal candidate thrives in a team atmosphere, takes pride in his/her
work, and is committed to a highly proactive, process-driven approach. They
must possess excellent programming skills and be customer oriented. Ideally,
a motivated, self-driven, and results oriented team player with the ability
to learn and adapt quickly to new environments... 阅读全帖 |
|
n**********9
发帖数: 2 | 4 40岁不到，好不容易拿到教授职称了，想去米国做做博士后，但是感觉这几年主要是发
了些SCI（10 papers写作能力大有长进）（主要是近5年全天阅读ENGLISH papers），
拿了些NSFC（more than one million），试验基本上是研究生，动手能力差些(只会一
些基本的，见下)，但又不甘心，还是想去米国，怎么办？
难道又要做试验，积累一些动手能力？？？？
专业是生物化学与分子生物学
主要技能：
1）饲料和食品营养成分分析（CP、CF、NDF、ADF等）和传统的消化代谢试验
2）primer design,PCR,RT,Real-time PCR,DNA and RNA extractions,gel
eletrophseis
3）sequences analysis,basic bioinformatics
4)RFLP,SSCP
5)Clone
40岁还算年轻，不去后悔，现在去嘛又怕自己实验室动手能力差呀？
欢迎拍砖 |
|
r*********r
发帖数: 6 | 5 “细菌变化”到底是指细菌种类的变化，还是数量的变化？
由于你的样品是许多混合的细菌，所以不能直接以SANGER法测序混杂的PCR产物。可以
用DGGE、SSCP、克隆、或pyrosequencing等方法，各有利弊，具体要看你想解决什么问
题。 |
|
c*******e
发帖数: 5818 | 6 先谢谢大家，我去研究研究你们的建议。
我的问题是PCR是mixture，估计也就<10%的 indel，所以要灵敏度高些的方法。
我现在想跑一个page gel，因为indel估计要induce size difference，这个要是行，
比较简单，直接对比WT。想过SSCP，但要麻烦些。 |
|
