g*********9
发帖数: 3528 | 1 It is clearly stated on its website......
See background:
"Stat3 isoform expression appears to reflect biological function as the
relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend
on cell type, ligand exposure, or cell maturation stage (10). It is notable
that Stat3β lacks the serine phosphorylation site within the carboxy-
terminal transcriptional activation domain (8)." |
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n***w
发帖数: 2405 | 2 Is this a pY705 STAT3?
It may be recognizing both serine and tyrosine phosphorylated stat3. |
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s**x
发帖数: 138 | 3 not stat3 luci
any1 has it? |
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m**********2
发帖数: 57 | 4 请问有什么通用或者常规的观察actived JAK/STAT3的functinal assay么?
比如说促进细胞分化之类的? |
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i*********0
发帖数: 915 | 5 cell signaling ab for p-stat3. two bands.
what is the meaning of the lower bands? isoforms? or modification? |
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i*********0
发帖数: 915 | 6 I am using Phospho-Stat3 (Tyr705) (#9145) from cell signaling.
I favor the answer of small isoforms. Maybe they have different functions.
Thanks a lot, guys, for the answer. |
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s********r
发帖数: 312 | 7 我用的BD的Y705，非常好，就一条带。你这个大小差别有10kd了吧，不像是stat3 beta |
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i*********0
发帖数: 915 | 8 Just got the reply from the CST. It seems the lower band is isoform beta.
Dear @@@@,
Thank you for your inquiry regarding our Phospho-Stat3 (Tyr705) (D3A7) XP&#
174; Rabbit mAb #9145. The top band, at 86kDa, represents Phospho-
Stat3alpha. The bottom band, at 79kDa, represents Phospho-Stat3beta isoform
. |
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L******r
发帖数: 141 | 9 All the materials were taken from Administrative Decisions (Aliens with Extraordinary Ability/ Outstanding Professors and Researchers). 所以这些话是权威!!!
I downloaded All the AAO decisions for the past three years. I read one by one for a total number of more than 200 decisions. I took good paragraphs from recommendation letters which IOs thought have met criterion of contribution. Because the works were from PDF files, I had to reformat them into word file.
It took me many hours to do the work and I... 阅读全帖 |
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d*******s
发帖数: 61 | 10 不想回国融入那种环境，就因为自己对科研还有真爱。
◇◇新语丝(www.xys.org)(newxys.com)(xys10.dxiong.com)◇◇
上海交通大学医学院附属仁济医院洪洁多篇论文图片造假
上海交通大学医学院附属仁济医院的洪洁多篇论文存在图片造假。
（1）洪洁以第一作者发表的Cancer Research论文(Bile Acid Reflux
Contributes to Development of Esophageal Adenocarcinoma via Activation
of Phosphatidylinositol-Specific Phospholipase Cγ2 and NADPH Oxidase
NOX5-S)，图5B和图4B使用了相同的凝胶电泳图片，是刻意修饰之后试图作为不
同实验结果。而图6A和图6B同样存在这样的问题。
（2）洪洁作为共同通讯作者发表的Journal of Pathology论文因图片造假
问题被撤稿：
http://onlinelibrary.wiley.com/doi/10.1002/path.4179/abstract
R... 阅读全帖 |
|
发帖数: 1 | 11 【 以下文字转载自 Biology 讨论区 】
发信人: dynabeads (dynabeads), 信区: Biology
标 题: 转XYS：上海交通大学医学院附属仁济医院洪洁多篇论文图片造假
发信站: BBS 未名空间站 (Sun Aug 5 12:00:39 2018, 美东)
上海交大，呵呵
◇◇新语丝(www.xys.org)(newxys.com)(xys10.dxiong.com)◇◇
上海交通大学医学院附属仁济医院洪洁多篇论文图片造假
上海交通大学医学院附属仁济医院的洪洁多篇论文存在图片造假。
（1）洪洁以第一作者发表的Cancer Research论文(Bile Acid Reflux
Contributes to Development of Esophageal Adenocarcinoma via Activation
of Phosphatidylinositol-Specific Phospholipase Cγ2 and NADPH Oxidase
NOX5-S)，图5B和图4B使用了相同的凝胶电泳图片，是刻意修饰之后试图作为不
同实验结果。而图6... 阅读全帖 |
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l*****7
发帖数: 8463 | 12 Cancer researcher retracts 19 studies at once
with 23 comments
jcheng
Jin Cheng
A former cancer biologist at the Moffitt Cancer Center in Tampa, Florida has
retracted 19 papers from a single journal.
Jin Cheng, who studies how ovarian cancer develops, withdrew 19 papers from
the Journal of Biological Chemistry originally published over the last 15
years, and corrected another. All of the retractions are for image
manipulation.
For example, here’s the notice for “Activation of phosphatidylinosito... 阅读全帖 |
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y***u
发帖数: 1467 | 13 title: STAT3 gene polymorphisms and susceptibility to non–small cell lung
cancer
Pls send me your CV to y*****[email protected] if you are interested in reviewing
it. |
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c**********c
发帖数: 81 | 14 来美国好多年了，一作发了三篇paper, 最早的10年发的，到现在也没攒几个citation
，只有靠review来加分了。发了很多邮件给editor，都没有回复的。跪求前辈们能给我
一些审稿机会，十分感谢。（之前就review过两次，都是别人给的机会）
研究方向：neurodegenerative disease
1. HIV 引起的神经性疾病 病理研究（Molecular mechanism of HIV-associated
neurodegeneratiove disease)
2. 神经干细胞发育和分化 （neuron stem cell development and differentiation）
3. 信号转导在神经分化中的作用，例如：Notch, Stat3, Wnt, mTOR, MAPK 等等（
Signaling transduction and its role in neuron stem cell development and
differentication)
4. 大脑衰老 （brain aging）
5. 星型细胞瘤和星型细胞... 阅读全帖 |
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p********3
发帖数: 5750 | 15 再让你长长见识：大家看和文献3-6相关的那句话，专门提出了中药的肾脏毒性问题，表明实际上的肾脏毒性比原先预期想象的还要普遍。
大家没有忘记在对Herb Medicine管制更严格的欧盟对中药 Ban了吧。 我上篇文献里面说了欧盟对Herb的管理对美国严格， 谈得比较细，有兴趣的可以仔细阅读。
Author
Marc E De Broe, MD, PhD Section Editor
Gary C Curhan, MD, ScD Deputy Editor
John P Forman, MD, MSc
Last literature review version 19.2: May 2011 | This topic last updated:
June 15, 2010
INTRODUCTION — In 1991, physicians in Belgium noted an increasing number of
women who presented with acute, often near end-stage renal disease
following expo... 阅读全帖 |
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t********e
发帖数: 1169 | 16 lenovo outlet又有新货， 看到一个z580, i7-3612qm, 8G, 1TB硬盘才三百多， 立马
硬了，精虫上脑立马下单买了
结果发现z580真心屌丝啊， 没有gigabit网卡，没有stat-iii，本来还想接个intel
ssd上去，咳哟，只能local卖了， nnd |
|
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t********e
发帖数: 1169 | 18 本来想自用的， 但看来只能local出了， 争取能赚个250把
那我的intel 330要用在哪？ |
|
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s****c
发帖数: 11300 | 20 其实outlet卖的电脑自带的ssd硬盘 比如180G这种型号就是intel的330 现在新的主流
硬盘除了intel没有其他有这种奇葩容量
挑这种的买没错 |
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t**t
发帖数: 27760 | 21 T400显然不支持STAT 3
你就是买了STAT3的SSD，也只能当SATA2用。 |
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x*****g
发帖数: 42 | 22 是这片吧？
Inhibition of JAK2/STAT3-Mediated VEGF Upregulation under High Glucose
Conditions by PEDF through a Mitochondrial ROS Pathway In Vitro
Zhi Zheng,1,2 Haibing Chen,2,3 Hui Zhao,1 Kun Liu,1 Dawei Luo,1 Yongdong
Chen,1 Yihui Chen,1 Xiaolu Yang,1 Qing Gu,1 and Xun Xu1
From the 1Department of Ophthalmology, Shanghai First People's Hospital
Affiliated Shanghai Jiao Tong University, Shanghai, China; 3Shanghai
Clinical Center for Diabetes, Shanghai Diabetes Institute, Department of
Endocrinology an |
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n***w
发帖数: 2405 | 23 嗯。我觉得你说得对。
你们看这样写怎么样？我觉得说得是不是有点刻薄。。。
Hi, Dr. Xu,
Today I read through your paper titled "Inhibition of JAK2/STAT3-mediated
VEGF Upregulation under High Glucose Conditions by PEDF through a
Mitochondrial ROS Pathway In Vitro" which has been published in
Investigative Ophthalmology & Visual Science, January 2010.
I found the two figures from Fig 7, which are from ELISA and RT-PCR,
respectively, are exactly the same, except for the replacement of names of x
- and y- axes.
I have no offense to you t |
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Z**********g
发帖数: 222 | 24 替同学急求paper一篇:
PMID: 21056449
Virology. 2010 Nov 4. [Epub ahead of print]
Shp2 suppresses PyMT-induced transformation in mouse fibroblasts by
inhibiting Stat3 activity.
发至: c************[email protected],包子谢之！ |
|
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W****C
发帖数: 1937 | 26 我估计你是要ｐｒｏｍｏｔｅｒ上有ｓｔａｔ３ ｃｏｎｃｅｎｓｕｓ ｂｉｎｄｉｎ
ｇ ｓｉｔｅｓ的质粒， addgene里没查到， 我只有ｍｉｒ２１的， 文献上说有
一个ｓｔａｔ３ ｓｉｔｅ |
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m***f
发帖数: 1622 | 27
扯淡呢，看看高最近两年发表的文章吧
CELL STEM CELL, JBC, STEM CELL, PNAS，都是很不错的
就这样还不能PROMOTION? 在美国都可以tennure了
1. Wu T., Wang H., He J., Kang L., Jiang Y., Liu J., Zhang Y., Kou
Z., Liu L., Zhang X., Gao S. (2011) Reprogramming of trophoblast stem
cells into pluripotent stem cells by Oct4. Stem Cells 2011 Feb 8.
[Epub ahead of print].
2. Kang L., Wu T., Tao Y., Yuan Y., He J., Zhang Y., Luo T., Kou Z.,
Gao S. (2010) Viable mice produced from 3-factor induced pluripotent
stem (iPS) cells through tetrapl... 阅读全帖 |
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n***w
发帖数: 2405 | 28 没看懂。。。
JAK/STAT activation就是phosphorylation什么的吧。。。
至于体现到具体什么phenotype，看你想研究什么了。。。 |
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m**********2
发帖数: 57 | 29 我的意思是Active JAK/STAT pathway出现某种phenotype，
而这种phenotype出现表明是激活JAK/STAT pathway 导致的。
Active JAK/STAT能够导致某种function assay or phenotype.
我现在是想加入某种drug出现某种function assay or phenotype.
说明drug是active JAK/STAT,
当然WB观察phosphorylation stat也是一种方法，但是只是从protein level。
我现在是想从功能level观察。想问问是不是有实践证明的Active JAK/STAT 导致的某
种function。
比如说某种细胞的分化，这样我加入drug，细胞出现分化，说明可能是active JAK/
STAT。 然后我再测量phosphorylation stat level increase，这样就比较完整了 |
|
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c****l
发帖数: 1086 | 31 WT stat3 can transform a cellline? do you have reference? |
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n***w
发帖数: 2405 | 32 STAT1 and STAT3 phosphorylation by porins are independent of JAKs but are
dependent on MAPK pathway and
plays a role in U937 cells production of interleukin-6.
PMID: 17258468
Please email to n***[email protected]
I appreciate your help. |
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d*******s
发帖数: 61 | 33 看过该网站回复人的帖子后，发现原来造假问题是异常严重的：WB里面，GAPDH的一张
图片被反复用在多个图中；而且在他们后来发表的其它 8 篇论文中，还存在相同的问
题！这是很明显的蓄意作假了。这些论文在下面用黑体标明：
7 Responses:
Well that was easy….In the v282#17p12363 paper, look at the GAPDH bands in
Figure 3A. Now go back to Figure 2C and look at the GAPDH bands. Lanes 4-5-6
are the same. They duplicated the blot.In the v283#29p20045 paper, Fig 3A
right panel GAPDH blot contains the same 2 bands as Fig. 5D.So, the “
mistake” appears to involve using the same GAPDH blot for different
experiments. ... 阅读全帖 |
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n***w
发帖数: 2405 | 34 I am not sure about nuclear factor.
I guess transcription factor and nuclear transcription factor are
interchangeable in most cases.
They can be expressed and latent in the cytoplasm and translocate to the
nucleus upon activation, for example, STAT3, NF-kB.
Also they can be nuclear encoded but transported to mitochondria to direct
transcription like Tfam. |
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s**x
发帖数: 138 | 35 哪位知道哪里可以要到pLucTKS3？ 文过几个人，没会音。多谢了 |
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p*****u
发帖数: 191 | 36 问一下James Turkson或者Richard Jove |
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D******9
发帖数: 2665 | 37 could be a small isoform. there are many reports about small isoforms of
Stat family. however, some researchers think they are caused by protein
degradation during cell lysate preparation. |
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l*******i
发帖数: 153 | 38 首先有一个概念要明确：目前说的ES细胞，都是in vitro culture的，与in vivo的不
一样。
ES细胞分为naive和prime两个stage
1)naive stage：ES colony在形态上看起来更“致密”，呈三维结构，对胰酶不敏感（
即胰酶消化不会诱导其凋亡）。对小鼠ES而言，naive的ES来源于inner cell mass(ICM
)。此stage的ES self-renewal更迅速，population double大概只需要12-14小时。对
于其self-renewal的维持需要LIF，stat3,BMP4等。雌性小鼠naive ES的另一个显著特
点是X chromosome reactivation,而在其他类型的细胞中有一条X chromosome是失活的
。从全能性角度来讲，naive ES是真正具有全能性的，能形成完整的个体。
2）prime stage：对小鼠而言，其来源于postimplantation epiblast,是naive stage
的下一个阶段，其形态看起来与naive ES明显不一样，呈“二维”结构，即colony... 阅读全帖 |
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S**********e
发帖数: 1789 | 39 严重不同意。近些年来美国的中国博士很多水平很高， 英文也不差。
Shideng Bao, Ph.D. 厦门大学博士， 他差不多是cleveland clinic最有潜力的了
Associate Staff
Guryanova OA, Wu Q, Cheng L, Lathia JD, Huang Z, Yang J, MacSwords J, Eyler
CE, McLendon RE, Heddleston JM, Shou W, Hambardzumyan D, Lee J, Hjelmeland
AB, Sloan AE, Bredel M, Stark GR, Rich JN, Bao S. Non-receptor tyrosine
kinase BMX maintains self-renewal and tumorigenic potential of glioblastoma
stem cells by activating STAT3. Cancer Cell 2011; 19:498-511.
Huang Z, Wu Q, Guryanova OA, Cheng... 阅读全帖 |
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g**********y
发帖数: 423 | 40 need the pdf of this paper. Thanks much!
http://www.thelancet.com/journals/lancet/article/PIIS0140-6736(
A strong correlation between expression of Wntless and of human epidermal
growth factor receptor 2 in gastric, ovarian, and breast cancers suggests a
novel-signalling pathway involving NFκB and STAT3 |
|
w*********n
发帖数: 439 | 41 Leptin increases HER2 protein levels through a STAT3-mediated up-regulation
of Hsp90 in breast cancer cells
Molecular Oncology
Volume 7, Issue 3, June 2013, Pages 379–391 |
|
D*****y
发帖数: 95 | 42 同意midwestPstD前辈的观点，但我想做一些补充。
除了High Throughput Screening之外，其实还有两种drug design的方法， ligand-
based 和structure-based。前者是在你知道一个靶标有很多结合的小分子时，可以通
过一些软件（例如CoMFA）计算得到一个pharmacophore，然后去搜索具有这个
pharmacophore的其它小分子，再通过实验的screening assay，以期找到活性更好的
leads。
structure-based是指基于靶标的空间三维结构（真实的或homology modeling）进行
rational design，主要包括Virtual Screening，即使用一些软件针对蛋白的binding
pocket （active or allosteric site）进行商业化成百万上千万小分子的docking，
然后通过score和经验挑选排名靠前的candidates，买来做screening assay，筛选到
hit之后，再进行optimization。structure-bas... 阅读全帖 |
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o******u
发帖数: 117 | 43 Would some one help me download this paper?
Journal: Diabetes. 2014 Dec 31. pii: DB_141225. [Epub ahead of print]
Paper title: Metformin inhibits monocyte-to-macrophage differentiation via
AMPK mediated inhibition of STAT3 activation: Potential role in
atherosclerosis
Email: [email protected]
/* */
Thanks a lot! |
|
发帖数: 1 | 44 最近查询文章，发现跟帕金森病相关的LRRK2研究一个有趣现象，其中有一篇是现任北
理工的庆宏教授发表的LRRK2能够直接结合a-Synuclein并且磷酸化Serine-129位点。
但是后续大量的综述讨论的时候提到他这篇文章时说this is the only one paper
show LRRK2 can phosphorylate S129-synuclein。我看他发表的这篇BBRC已经被引用
快超100次了，如果文章的结论有问题，那岂不是严重阻碍了关于LRRK2-G2019S的研究
？大伙有兴趣的可以来共同探讨，或者找庆教授讨论。电子邮件：[email protected]/* */
个人简历
教育背景：
1993.9-1996.7：博士，广州中山医科大学，神经生物学
工作经历：
2006.2- 至今：北京理工大学生命科学与技术学院，教授
2001.9 – 2006.2：英属哥伦比亚大学精神病学系，脑研究中心，研究员
2000.7 – 2001.8：加拿大萨斯喀彻温大学，神经精神病学系，博士后
1997.7- 2000.6：美国埃默里大学医学院，放射肿瘤系与细胞... 阅读全帖 |
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w******y
发帖数: 4871 | 45 这位同学，Hyper IgE syndrome (Job's syndrome)是俺们科看的病，它的临床表现不
是这样的。IgE一般超过2000单位。
最常见的是STAT3的基因变异。 |
|
C******r
发帖数: 790 | 46 那么多hormone和molecule 都对food intake有影响
leptin, insulin, ghrelin, GLP1, NPY, CART...
PIP3, AMPK, STAT3, CaMKK2, ......
这些家伙还有协同或抑制效应，无法无天无边无际的一堆乱麻。。。 |
|
