a***a
发帖数: 40617 | 1 我跳出来挑刺。。。sucrose不到70.。。应该是按照glucose算的 |
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z******7
发帖数: 104 | 2 果糖fructose是单糖...蔗糖sucrose分解成一分子葡萄糖glucose一分子果糖... |
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b****i
发帖数: 11322 | 3 again, from wiki (and we all know how evil fructose is):
Typical honey analysis:[45]
Fructose: 38.2%
Glucose: 31.3%
Maltose: 7.1%
Sucrose: 1.3%
Water: 17.2%
Higher sugars: 1.5%
Ash: 0.2%
Other/undetermined: 3.2% |
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b****i
发帖数: 11322 | 4 let me bring out wiki, yet, again:
fructose absorption:
The mechanism of fructose absorption in the small intestine is not
completely understood. Some evidence suggests active transport, because
fructose uptake has been shown to occur against a concentration gradient.[23
] However, the majority of research supports the claim that fructose
absorption occurs on the mucosal membrane via facilitated transport
involving GLUT5 transport proteins. Since the concentration of fructose is
higher in the lu... 阅读全帖 |
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b****i
发帖数: 11322 | 5 from wiki:
Typical honey analysis:[45]
Fructose: 38.2%
Glucose: 31.3%
Maltose: 7.1%
Sucrose: 1.3%
Water: 17.2%
Higher sugars: 1.5%
Ash: 0.2%
Other/undetermined: 3.2% |
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b*********t
发帖数: 877 | 7 glucose（葡萄糖，简称g）和fructose(果糖,简称f）都是单糖，但代谢上有着本质区
别。前者直接肠吸收,变成糖原，能量，用作消耗。消耗不够的才变脂肪。
f呢，肠吸收不行，只能肝降解，大部分不能变糖原，而是直接降解成脂肪元，用作合
成脂肪。更容易增肥，而且增大肝负担。而且f比g甜很多，容易上瘾。
淀粉都是多糖，也就是长链的g。而蔗糖sucrose是双糖，一半g一半f。所以同样质量下
哪个更有害更长胖，一目了然。 |
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z*h
发帖数: 773 | 8 In 2003, four United Nations agencies (including the World Health
Organization (WHO) and the Food and Agriculture Organization (FAO))
commissioned a panel report about the UN dietary recommendation. It state
that (i) carbohydrates in total should represent between 55% and 75% of the
calorie intake and (ii) the total of free sugars, including all
monosaccharides (e.g., glucose, fructose), disaccharides (e.g., sucrose,
lactose) in the processed food and natural food (e.g., honey, syrups, fruit
jui... 阅读全帖 |
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b***i
发帖数: 10018 | 9 水果里的fructose含量远远不如人工提炼出来的high fructose corn syrup和sucrose
，而且有fiber,vitamin, mineral，所以ok。HFCS因为便宜，被加到绝大多数的工业化
食品里，是导致metabolic syndrome(肥胖，心脏病，糖尿病，高血压等）的主要元凶。 |
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F***I
发帖数: 4170 | 10 Monosodium glutamate
From Wikipedia, the free encyclopedia
Jump to: navigation, search
This article is about the chemical compound. For its use in food, see
glutamic acid (flavor). For glutamic acid in general, see glutamic acid.
Monosodium glutamate
Chemical composition of monosodium glutamate
Structure of monosodium glutamate
Crystalline monosodium glutamate
IUPAC name[hide]
Sodium 2-Aminopentanedioate
Identifiers
CAS number 142-47-2 Yes
PubChem 85314
ChemSpider 76943 Yes
UNII ... 阅读全帖 |
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b*s
发帖数: 82482 | 11 来自主题: LeisureTime版 - 关于中餐 Umami has a mild but lasting aftertaste difficult to describe. It induces
salivation and a sensation of furriness on the tongue, stimulating the
throat, the roof and the back of the mouth (for review Yamaguchi, 1998).[20]
[21] By itself, umami is not palatable, but it makes a great variety of
foods pleasant especially in the presence of a matching aroma.[22] But like
other basic tastes, with the exception of sucrose, umami is pleasant only
within a relatively narrow concentration range.[20] The ... 阅读全帖 |
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l***y
发帖数: 4671 | 12 手头正在做 molecular reporter，天天摇菌呢。
e coli 在 exponential phase 大约 17 分钟一代。
http://textbookofbacteriology.net/growth_3.html
Table 2. Generation times for some common bacteria under optimal conditions
of growth.
Bacterium Medium Generation Time (minutes)
Escherichia coli Glucose-salts 17
Bacillus megaterium Sucrose-salts 25
Streptococcus lactis Milk 26
Streptococcus lactis Lactose broth 48
Staphylococcus aureus Heart infusion broth 27-30
Lactobacillus acidophilus ... 阅读全帖 |
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j******n
发帖数: 21641 | 13 荠菜根白色。茎直立，单一或基部分枝。基生叶丛生，埃地，莲座状、叶羽状分裂，不
整齐，顶片特大，叶片有毛，叶耙有翼。茎生叶狭披针形或披针形，基部箭形，抱茎，
边缘有缺刻或锯齿。全株含草酸（oxalic acid），酒石酸（tartaric acid），苹果酸
（malic acid），丙酮酸（pyruvic acid），对氨基苯磺酸（sulfanilic acid），精
氨酸（arginine），天冬氨酸（aspartic acid），脯氨酸（proline），蛋氨酸（
methionine），亮氨酸（leucine），谷氨酸（glutamic acid），甘氨酸（glycine）
，丙氨酸（alanine）），胱氨酸（cystine），半脱氨酸（cysteine），蔗糖（
sucrose），山梨糖（sorbose），乳糖（lactose），葡萄糖胺（glucosamine），山梨
糖醇（sorbitol），甘露醇（man－nitol），侧金盏花醇（adonitol）以及钾、钙、钠
、铁、氯、磷、锰。还含二氢非瑟素（dihydrofisetin），山奈酚-4’-甲醚（
kaempfero... 阅读全帖 |
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m***j
发帖数: 9290 | 14 再来一种，就更便宜了。
3。Beef Hearts, Beef Kidneys, Beef Lungs, Whole Wheat Flour, Brown Rice,
Wheat Bran, Beef Fat (preserved with Mixed Tocopherols and Citric Acid),
Sucrose, Flaxseed Meal, Calcium Carbonate, Sodium Chloride, Modified Food
Starch, Sodium Phosphates, Water, Brewer's Yeast, Dried Kelp Meal, Lecithin,
Natural Smoke Flavor, Sorbic Acid, Glycerin, Rosemary Extract, Canola Oil,
Choline Chloride Supplement, Ferrous Sulfate, Riboflaven Supplement, Vitamin
B-12 Supplement, Vitamin A Acetate, Foli |
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l*****f
发帖数: 13466 | 16 来自主题: ChineseClassics版 - 新春庙会 wiki:
sucrose
C12H22O11 |
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d**i
发帖数: 1606 | 17 来自主题: ChineseClassics版 - 新春庙会 sucrose又是啥？糖的学名么？ |
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f*******c
发帖数: 94 | 18 you are right, DOE is not idiots, but this pathway can only work if
follwoing problem can be fixed:
1) a energy efficient pretreatment method to handle >30% biomass and convert
all cellulose + hemicellulose to simple sugars (glucose, fructose, sucrose
and xylose), those methods include solid acid catalysis, hydrothermo
hydrolysis or other advanced tech.
2) fast and energy saving separation method to remove lignin from biomass
hydrolysate
3) energy saving separation method for ethanol purificatio |
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d********a
发帖数: 3698 | 19 chen贴了可不是把你引来了么?
抛砖引玉么。
未必没用，我看玉米转化糖浆就不错了。
convert
sucrose |
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c*******n
发帖数: 1648 | 20 呵呵，不争了，这个我承认是外行。所以无法评价啊。
convert
sucrose
biomass
make |
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t******n
发帖数: 6861 | 22 酒精也就是个近期目标，从第一代转第二代很多infrastructure可以用现成的，关键是
还是有incentive和政府补贴，否则赚不了钱
长期目标应该是hydrocarbon
convert
sucrose |
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s**l
发帖数: 11983 | 23 strongly recommend this project and its website:
http://www.nongmoproject.org/
GMOs, or “genetically modified organisms,” are plants or animals created
through the gene splicing techniques of biotechnology (also called genetic
engineering, or GE). This experimental technology merges DNA from different
species, creating unstable combinations of plant, animal, bacterial and
viral genes that cannot occur in nature or in traditional crossbreeding.
For consumers, it can be difficult to stay up-to-dat... 阅读全帖 |
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发帖数: 1 | 24 HOW IDO WORKS
https://www.youtube.com/watch?v=BTVtaB2nU8w&list=
PL0eUjNQs8Qf9SK214nZuK41ffkxvKeA0A
http://idolinguo.org.uk/works.htm
INTERNATIONAL UNDERSTANDING
The idea of an international language is simply to enable people who have no
other language in common to understand one another. Ido (pronounced "ee-doh
") was developed by linguists and scientists over a number of years. It is
the most practical language to be invented, and its usability has been
demonstrated many times, both in writing... 阅读全帖 |
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x***g
发帖数: 923 | 25 问题很多，
1, sucrose脱水过夜是不够的，我记得我是10%，20%，30％梯度脱的
好像有点多余，不过直接用30%的脱，中间也要换液。
2, 冷冻很讲究，乙醇干冰最好，实在不行液氮也凑合了，冻得不好就碎了
3, 起皱是贴片的功夫，这个得练，熟了就不起了
4, 本底高看你的操作可能是wash的不够，应该是摇床5min，三次
5, 掉片子是载片处理的不好。先泡酸，然后泡乙醇，最后泡明胶或防脱片剂 |
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s******y
发帖数: 28562 | 26
Because radioactive detection is very sensitive.
If you use cell-free in vitro translation kit, you will stick a lot of
ribosomes on the NTA-resin, and the unfinished peptides will show up as
smear on the gel.
What you need to do, is to use high speed centrifugation (at least 144,000
xg) on a 20% sucrose cushion to separate the ribosome fraction (go to bottom
) from the complete proteins (remain on the supernatant).
Then, you can use the NTA resin on the supernatant. |
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e****s
发帖数: 1125 | 27 I see.
I cleaned up the samples by ultracentrifuge at 100,000rpm for 30 mins (
without sucrose) following by gel filtration to remove free labeled AAs.
the problem is the pulldown assay signal is very low. I may try both NTA-Ni
pulldown and IP to see which condition give me a better result.
bottom |
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h****g
发帖数: 439 | 28 不同组织处理和固定方式的对FITC-Dextran的影响：
1，tumor tissue-OCT-section-acetone: fail
2, tumor tissue-OCT-section-PFA:fail
3, tumor tissue-PFA-sucrose-OCT-section: work!
看来FITC-dextran 和YFP一样，要用PFA固定，然后再OCT |
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h*****d
发帖数: 210 | 29 加蛋白probe是不一样的， it is smear, but there is not a single distinct
probe. Could have some reasons for this, first of all, probe denaturing and
annealing is not good, I bought oligonucleotide, because it is highly
purified. the free probe is also smear which is about half length of the
smear with protein.
secondly, when I extract nuclear protein, I just use 250M sucrose in the
extraction buffer for nuclei and no further gradient centrifuge thereafter.
High/low salt method for getting nuclear protein( |
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w*****n
发帖数: 107 | 30 why don't you perform polysome analysis using sucrose gradients? |
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n********k
发帖数: 2818 | 31 Could any friends recommend nice and simply ways to look at mature neuron
dendritic morphology in hippocampus (CA regions and DG as well). my Cre is
so bright and GFP is so weak, hard to tell...not sure whether my sections
still can go for Golgi staining since we perfused with PFA and then sucrose
and then section at 30um...I am thinking to go with calbindin... |
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s******y
发帖数: 28562 | 33 Depnding on really what do you need to do.
If you want to simply seperate the agregate from soluble protein,
you can buy those 10 cm disposable columns and pack the resin by yourself.
It is not too difficult.
Or, you can just do a ultracentrifugation with a sucrose cushion to seperate
the aggregate. |
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s******y
发帖数: 28562 | 34 1. memrane filter? I would recommend using the inverted filter set in order
to protect the activities of the proteins.
2. high speed centrifugation with sucrose cushion?
3. a short, fat, gel filtration column? |
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s******y
发帖数: 28562 | 36 他这个是把中学生理卫生当作宝典用
人体对于单糖吸收效率最高，但是肯定是可以吸收低聚糖 (比方说lactose, maltose)
，甚至某些多糖的。
人体对于蔗糖（sucrose)的直接吸收效率倒是比较低，估计方舟子就是没有好好
上大学的代谢课，然后把这个蔗糖的例子误当作了所有二糖。 |
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s****a
发帖数: 454 | 37 大家说说heparin是不是必须的呢?有的人用没有的人不用,是跟细胞类型有关吗,
还有,我看的文献大多用sucrose gradient,今天跟一个老师请教,他没有做过polysome
的抽提,做过别的.他说是最好用iodixanol gradient.有经验的来说说? |
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w*****n
发帖数: 107 | 38 Heparin helps to protect RNA by keeping RNases busy
sucrose gradient is the standard to do polysome analysis. |
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h**********r
发帖数: 671 | 39 以前好好的。接着几次跑胶小片段都有点弥散，不知道原因。左图是筛克隆时的双酶切
，质粒是按《分子克隆》的碱裂解法提的。右图借用别的lab的qiagen的试剂盒提的然
后双酶切。问题也可能出在loading dye上。按《分子克隆》配的6X,40% sucrose加上
两种染料。EB是直接加在胶里的。大家看看吧，多谢！ |
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s******y
发帖数: 28562 | 40 Native gel and sucrose gradient centrifugation |
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s******y
发帖数: 28562 | 41 http://www.piercenet.com/browse.cfm?fldID=02020104
Substances compatible with the Thermo Scientific Coomassie Plus Protein
Assay. The following is a short list of compatible substances and their
concentrations that the BCA Protein Assay can tolerate. For a more complete
list please refer to the instruction booklet for the product or view our
Table of Compatible Substances for a comparison of substance compatibilities
among several different protein assay methods.
Substance Compatible Conc. ... 阅读全帖 |
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s*********t
发帖数: 600 | 42 用420mM KCl抽提的细胞nuclear extract。
用来做IP，IP条件是盐浓度150mM。
可以直接把nuclear extract稀释到150mM KCl，蛋白浓度稀释到1ug／ul做IP吗？
还是最好先透析过夜到150mM KCl (或者还同时+ 20% sucrose浓缩)？
透析会产生好多沉淀啊，但是直接稀释却不会。
所以，这两个有什么区别？
谢谢指点。 |
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s*********t
发帖数: 600 | 43 我还加了20％ sucrose在透析buffer，是不是这样更好？ |
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l*h
发帖数: 4124 | 44 If you calculate the centrifugal force (in g) and know the sedimentation
rate of your virion, you can have an educated guess about the conditions you
need.
the
). |
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c****g
发帖数: 93 | 45 我们用这个lysis buff：
10 mM HEPES/KOH, pH7.6
1.5mM MgCl 2
10 mM KCl
5 mM EDTA
5 mM EGTA
250 mM Sucrose
Adjust pH 7.6 with 10N KOH
然后过过15次#7 needle，低速离心后上清基本是cytosol，无nuclear protein。
10
H3 |
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i****l
发帖数: 579 | 47 多谢美女！我试过这种瓶子和瓶盖，确实不怎么漏，就是我想做sucrose preference,
我们现在的笼子放两个瓶子就不能放进笼架了，真伤脑筋。
吧。 |
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b******s
发帖数: 193 | 48 same here.
I am also testing sucrose intake. Have to use water bottle supplied by mice
cage company.
it leaks a little bit, but generally not too bad |
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D*a
发帖数: 6830 | 49 测试sucrose的就不懂了，是说一瓶水一瓶糖水？这个测出来是测什么的？ |
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Z**********g
发帖数: 222 | 50 我也问个SA-beta gel染色的组织切片问题:
1. 关于切片,与制备普通冰冻切片一样吗(fixation,sucrose infiltration,OCT
embedding,cutting)?还是直接OCT embedding?
2. 冰冻样品(未切)或切片能在-80冰箱保存多久不影响染色?
谢谢 |
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