m****d
发帖数: 9307 | 1 游泳是不错,我觉得是最佳运动,不过我还是prefer太极,简单,不用换游泳服,不用找swi
m pool |
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h**********8
发帖数: 650 | 2 More than acetylation.
EMBO J. 2010 Jan 28.
Crosstalk between C/EBPbeta phosphorylation, arginine methylation, and SWI/
SNF/Mediator implies an indexing transcription factor code.
Kowenz-Leutz E, Pless O, Dittmar G, Knoblich M, Leutz A. |
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c********b
发帖数: 363 | 3 楼上的思路不错。
其实方法很多，但是比较简单的还是先crosslink，然后再用 harsh buffer把核裂开，
然后复性，最后该干嘛干嘛。
这里有一篇你可以参考：Rapid and phosphoinositol-dependent binding of the SWI
/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling
我自己用的protocol是这样的：
如果只是检测蛋白表达:
用High SDS buffer:
15 mM Tris pH 8.8, 5% SDS
0.3 M DTT, 15% Glycerol
我的是植物细胞,一般是liquid nitrogen grind之后加buffer,boil.如果是cell line,
collect细胞之后加上buffer 然后直接煮.除非是非常不稳定的蛋白,不然连protease
inhibitor都可以不加.
看到蛋白之后如果是做IP:
先crosslonk(用什么chemical得自己琢磨),然后把ripa的SDS含量加到1% ,... 阅读全帖 |
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l**********1
发帖数: 5204 | 5 同意 买买提 actin No. one faculty sunny day's 回复
BTW sunnyday 对
SMARCA2 相关的up and down stream realrive control transcription factors 熟
悉不?
SWI/SNF related, matrix associated, actin dependent regulator of chromatin,
subfamily a, member 2
----
Re:
发信人: sunnyday (飞鱼. 发胖中.飞不动了), 信区: Biology
标 题: Re: 什么是membrane ruffle?
发信站: BBS 未名空间站 (Tue Jul 3 11:46:03 2012, 美东)
就是细胞膜底下堆着高高一堆actin 把膜都拱起来了。 |
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T******T
发帖数: 3066 | 6 Have seen many I2C issues over the years, like everyone is saying matching
voltage rail is important, and tweaking the pullup resistors with respect to
your bus capacitive load will also help.
1) Check you SDA/SCL waveform, do you have timing violation induced by
excessive loading (spiky looking edges) ? If so, reduce SCL speed, increase
pull-up strength (not too much as that'll raise your effective VOL).
2) Check your SDA/SCL output swing from the processor, and the response
traffic output swi |
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r****o
发帖数: 1950 | 7 多谢，
大侠能解释一下SWI和RDI啥意思吗?
另外，还有个on-chip breakpoint，这个和hard breakpoint啥区别? |
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n*******c
发帖数: 501 | 8 是cavernoma吗？SWI 有什么表现？
哎，老大解释了就涨知识了，道听途说，有时失之毫厘，谬以千里，以后类似讨论还是
不要随便掺和比较明智 |
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i*********5
发帖数: 19210 | 9 今年 Caroline Steffen （Kona第五名） 骑的 P4 带的那个水壶好像还挺合适的。不知道她去哪里搞的。（还是那压根就不是个水壶？）
1 08:55:08 Wellington, Chrissie Feltwell NOR GBR 1:01:03 4:56:53 2:52:41
2 08:57:57 Carfrae, Mirinda Boulder CO USA 57:17 5:04:17 2:52:09
3 09:03:29 Cave, Leanda Tucson AZ USA 53:54 4:58:41 3:06:36
4 09:06:57 Joyce, Rachel London GRE GBR 53:56 4:58:57 3:09:55
5 09:07:32 Steffen, Caroline Spiez BE SWI 57:15 4:50:26 3:15:17 |
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i*********5
发帖数: 19210 | 10 1 09:15:54 103 Cave, Leanda 34 TUCSON AZ USA 56:03 2:09 5:12:06 2:25 3:03:13
2 09:16:58 101 Steffen, Caroline 34 SPIEZ SWI 57:37 1:48 5:06:49 2:37 3:08:
09
3 09:21:41 102 Carfrae, Mirinda 31 BOULDER CO USA 1:00:06 1:56 5:12:18 2:19
3:05:04
4 09:22:45 120 Tajsich, Sonja 37 SINZING GER 1:10:36 2:03 5:07:58 2:43 2:59:
27
5 09:22:57 105 Ellis, Mary Beth 35 SUPERIOR CO USA 56:06 1:59 5:08:06 6:18 3
:10:30 |
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d******0
发帖数: 22800 | 12 啊 我以为wow 和ewatches都是在fl的 那个主人叫啥lori的 楼主问的那个是南加的
可靠是可靠的 没啥问题 但是不知道是wow一家，你确认吗？
Wow的owner也是swi的owner |
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x*****n
发帖数: 3422 | 13
这个有东西可以搞定，大概$50~60预算，SWI-JACK
嗯，这就是我现在考虑的，crutchfield给的面板就是把剩下地方都封起来，连个
storage bin都没有，太浪费空间的，考虑自己diy一下，留个位置给GPS。 |
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S******t
发帖数: 8388 | 14 FA没问题的话我觉得可以。但要有耐心才行。
我当初如swi也是刚入市买，可惜没捂住，要不也差不多翻被了。 |
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g*****u
发帖数: 14294 | 15 CDNS
COOL
DELL
HRL
KRA
MTSN
OSUR
OXGN
PH
RBCN
STKL
SWI
VIVO
现在这个环境做多困难。
看看无妨。 |
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N***i
发帖数: 2063 | 17 Best-Rated Technology Stocks Updated June 23, 2010
http://www.nasd100.com/2010/06/bestrated-technology-stocks-updated-june-23-2010.html
Below are the best-rated Technology stocks based on average rating by Wall
Street analysts. Each analyst rating is assigned a number ranging from 1 to
5, with 1 being the highest rating (STRONG BUY) and 5 being the lowest
rating (STRONG SELL). Then the average is calculated to represent the
overall rating of a stock.
Ranking | Company (Ticker) | Average Rati... 阅读全帖 |
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y*****l
发帖数: 5997 | 18 整理一下，分成三类，大家看看想怎么筛选：
Best-Rated Technology Stocks
Strong Buy <1.5 Total: 80
http://finviz.com/screener.ashx?v=211&t=CTFO,JNGW,TSTC,CKSW,GILT,RTLX,CAJ,LPL,ACFN,ACTL,ADPT,AFOP,AGYS,ASYS,AXTI,CAMP,CPWR,DDIC,DSTI,EBIX,EFJI,EVOL,ICGE,LOCM,MIPS,MOSY,NVEC,OCNW,PKT,PLAB,PLXT,PRFT,PVSW,RADS,SABA,SCON,SPRT,STRM,TACT,VHC,VTRO,WYY,ARTG,LPSN,
Strong Buy 1.5-1.8 Total: 97
http://finviz.com/screener.ashx?v=211&t=NVMI,RDWR,ACCL,ACOM,ACTI,AMSWA,CBR,COHU,CRAY,DTLK,ESIO,GSIT,ININ,MENT,MSCC,NTWK,PKE,PLT,PSEM,QTM,RI... 阅读全帖 |
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P*****s
发帖数: 2619 | 19 我有！给个大包子吧 哈哈
ALXN
GNC
LULU
LQDT
KORS
QCOR
AAPL
ULTA
SWI
EC
INVN
MA
TDG
V
TSCO
CLR
FIRE
CELG
CMG
MNST
PCLN
MELI
HLF
ROST
VAL
NUS
TDC
TAL
FOSL
FAST
DLTR
SHOO
FFIV
SWKS
CVLT
CMI
GWW
PII
AGO
GOLD
CF
CXO
HMSY
CERN
ALGN
NVO
TJX
TCBI
EBIX
UBNT |
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