k**********g
发帖数: 989 | 1
Yes, performance is the main factor.
OpenCV is performance intensive because it has to process a lot of data. A
single image may have tens or hundreds of millions of bytes. Whereas an HTML
file may only be 0.1 megabyte or so.
https://github.com/Itseez/opencv/blob/master/modules/imgproc/src/imgwarp.cpp
Java can always use OpenCV's Java bindings.
OpenCV has been ported to many different architectures. In most
architectures, CPU-specific (and GPU-specific) optimizations and new
instruction sets ar... 阅读全帖 |
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y*******g
发帖数: 6599 | 2 objective-c, 居然还有@synthesize image = _image; ，，，有些年头了 |
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w*****s
发帖数: 122 | 3 [April 10, 2000] FlowML: A Format for Virtual Orchestras. Bert Schiettecatte (Vrije Universiteit
Brussel) reports on the development of an XML-based format for musical notations - 'FlowML'.
"FlowML is a format for storing audio synthesis diagrams, as used in various software synthesizers. It
offers a way to store synthesis instruments as a graph of reusable synthesis blocks (defined in the format
specification). It differs from existing languages for audio synthesis like CSound, |
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x**7
发帖数: 239 | 4 One client ask me appropriate candidates or individuals in the industry for
the following position.
Please contact hiring manager Nate Bass(n***[email protected]),if you
were interested.
----------------
Position Title: Natural Catastrophe Modeler
Location: Miami, FL
Reports To: Regional Catastrophe Manager and AVP
Position Summary:
This position plays a major role in the support of SCOR underwriters
in the Americas through the provision of catastrophe risk analytics... 阅读全帖 |
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x**7
发帖数: 239 | 5 One client ask me appropriate candidates or individuals in the industry for
the following position.
Please contact hiring manager Nate Bass(n***[email protected]),if you
were interested.
----------------
Position Title: Natural Catastrophe Modeler
Location: Miami, FL
Reports To: Regional Catastrophe Manager and AVP
Position Summary:
This position plays a major role in the support of SCOR underwriters
in the Americas through the provision of catastrophe risk analytics... 阅读全帖 |
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s******o
发帖数: 298 | 6 Scientists create amino acids in simulated interstellar environments.
by Vanessa Thomas
Two teams of scientists have been able to synthesize life's basic building
blocks by simulating the environment of interstellar space. This not only
suggests that the seeds of life on Earth might have come from space, but that
prebiotic ingredients could be everywhere.
"This finding may shed light on the origin of life itself," states Max
Bernstein, a scientist at the SETI Institute and NASA's Ames Research C |
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m******n
发帖数: 194 | 7 ok, 简单说一下：
DNA:
cDNA: is a single-stranded DNA complementary to an RNA, synthesized from mRNA
by reverse transcriptase from mRNA.
genomic DNA: DNA of the genome
(big different between cDNA and genomic DNA is in eukaryotes, genomic DNA
has a lot of junk (non-coding seq, satalitte DNA, etc) DNA.
while cDNA only contains the coding region.)
cDNA is used extensively to make cDNA library.
RNA:
mRNA: message RNA, RNA that codes for protein sequence.
tRNA: transfer RNA, about 70-120base pairs, used in |
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n***h
发帖数: 11 | 8 No. It's like the other way around...
Affymetrix chips use synthesized 25-base long oligonucleotides as probes,
including perfect match and mis-match probes. A set (16-20 ) of probes are
designated to detect one transcript. So all the probes on the chip stand
for known genes, which is nice for reproducibility and further analysis of
your data, while could be a limitation too: you can only detect the expression
level of the probes they provide.
I haven't worked with the so-called "arrays", but as |
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t*********r
发帖数: 31 | 9 转载请注明出处 （Transmitter）
也谈大牛系列之，Roger Tsien （三）
---------- 天才是怎样炼成的
（“与‘金庸’容易混淆的武侠小说作家有谁?答：金庸新 金庸巨 全庸 全
康 金康 金唐 金庸著 金庸名 金庸原”----From 百度。谨于此句向“lanceandfei”
致歉并致谢。“半庸全庸，江湖依然是江湖”）
在Richard Keynes的实验室待了三年（1975-1978）Roger顺利得到了PhD。于
1978-1981在Timothy Rink（Cambridge, UK) 的实验室做了短暂的postdoc。在Roger将
开始了他在Berkeley独立的实验室的工作前，他已经开始了不平凡的revolutionary的
工作-----trying to synthesize voltage sensitive chemicals to imaging action
potential。各位看官可能会问--“不对呀，Roger不是开始合成 |
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l******n
发帖数: 6 | 10 During SfN I read their poster and talked with the leading post doc and
students. I heard they actually synthesized the compound. The behavioral
data are also interesting. I think they are planning to submit another major
one soon. |
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w******e
发帖数: 1187 | 11 I hope I knew this earlier...:)
I guess you are talking about endosomal escape? or chemical conjugation
simply
abrogate the drug property?
I'm hooked with the simplicity of conjugating aptamer with siRNA. both can
achieve certain level of specificity; both can be synthesized in a single
run; the toxicity profile of ONs are widely available due to the gold
rush in anti-sense field... Yet it's a shame that you only have that few
aptamers to choose from (well, only 1 or 2, according to my knowledge |
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p*******r
发帖数: 4048 | 12 I actually had an idea. I wanted to reconstruct the ancient genome from
extent species, then I want to synthesize a new animal which should be
similar to ancient species. |
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f**u
发帖数: 346 | 13 嗬嗬，你说直接synthesize一个genome，我的理解是design一个新的物种，
个人认为应当从简单的细菌甚至病毒开始，直接作animal难度太大了。 |
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p*******r
发帖数: 4048 | 14 Of course, long term dream...
Really long term dream is to synthesize next generation human. :)
I want to figure out a kind of human that could live forever. |
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s******y
发帖数: 28562 | 15
定了，信号/
I guess in the first case you pulldown your protein and then analyze the
pulldown result by WB? In such case, it would look decent.
In the second case were you trying to pulldown in vivo synthesized protein
with isotope? Indeed, the signal/noise will look awful, because many
endogenous protein also have the radioactive label.
Well, if that's what you want, you probably will need to try the anti-6XHis
antibody. Unfortunately I don't know which antibody works for IP. You should
check this |
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a****o
发帖数: 1786 | 16 since mRNA level does not change, it is unlikely the stability of mRNA is
the cause. it is more likely to be translation efficiency or protein
stability changed. I suggest you to check the association of ribosome with
this mRNA first, then check if protein level is higher in a protease
deficient strain, another thing you can check is the de novo synthesis of
mRNA by ChIP. maybe only newly synthesized mRNA can be translated. Good
luck! |
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l*********s
发帖数: 5409 | 17 Nay, the 5-prime end of synthesized oligos is normally hydroxy group, you
need to order additional modification.
For annealing, water is also OK.Just throw oligo solutions in boiled water
and let it cool naturally.
|
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b******k
发帖数: 1874 | 18 so, if i am going to ligate two tandem short fragments (say, 50bp each):
synthesize 50nt oligo of Sense and antisense (with 5' phosphate group modifi
cation),
anneal them by boiling and cooling naturally,
then ligate the annealed fragments (ideally, with synthesizd 3' overhang fro
m first one, and 5' overhang from 2nd)by conventional T4 ligase system?
thanks. |
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k****l
发帖数: 279 | 19 For 1.3kb, it's cheap to synthesize. But it shouldn't be very difficult to
amplify. 24bp primer is too long, 18 is enough.
You can try epicentre fail-safe PCR kit. There are 12 buffers with different
Mg2+ and betaine combinations. Usually some of them should work. |
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a*****5
发帖数: 168 | 20 这个作者名单太威武了！
Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Daniel G. Gibson,1 John I. Glass,1 Carole Lartigue,1 Vladimir N. Noskov,1
Ray-Yuan Chuang,1 Mikkel A. Algire,1 Gwynedd A. Benders,2 Michael G.
Montague,1 Li Ma,1 Monzia M. Moodie,1 Chuck Merryman,1 Sanjay Vashee,1 Radha
Krishnakumar,1 Nacyra Assad-Garcia,1 Cynthia Andrews-Pfannkoch,1 Evgeniya A
. Denisova,1 Lei Young,1 Zhi-Qing Qi,1 Thomas H. Segall-Shapiro,1
Christopher H. Calvey,1 Prashanth P. Parmar,1 Clyde |
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a****d
发帖数: 1919 | 21 帮着贴一个过来，呵呵，前几天lab里面还在讨论这个呢。
First Self-Replicating Synthetic Bacterial Cell
First Self-Replicating, Synthetic Bacterial Cell Constructed by J. Craig
Venter Institute Researchers
ROCKVILLE, MD and San Diego, CA (May 20, 2010)— Researchers at the J. Craig
Venter Institute (JCVI), a not-for-profit genomic research organization,
published results today describing the successful construction of the first
self-replicating, synthetic bacterial cell. The team synthesized the 1.08
million base pair chrom |
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y******8
发帖数: 1764 | 22 The protein G B1 domain is about 8kd, and would be hard to synthesize.
Pratically, this is the only reason you need to ask for the material and
permission to start. Otherwise, you could just do it and wait for response.
Usually, people don't pay attention to nonprofit stuff. |
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M*****e
发帖数: 279 | 23 Can anyone elaborate on Xiaodong's SREBP?
SREBP transcription factors: master regulators of lipid homeostasis.
Is Xiaodong the first person who found some of transcription factors (like
SREBP) that can be synthesized as transmembrane (or membrane bound)
precursors, but become active transcription factors by intramembraneous
proteolysis?
It is now well-known that intramembraneous proteolysis plays an important
role in some signaling pathways, such as WNT (beta-catenin) and Notch.
If Xiaodong is t |
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s******y
发帖数: 28562 | 24 Int J Biol Sci 2009; 5:706-726 ©Ivyspring International Publisher
Research Paper
A Comparison of the Effects of Three GM Corn Varieties on Mammalian Health
Joël Spiroux de Vendômois1, François Roullier1, Dominique
Cellier1,2, Gilles-Eric Séralini1,3
We present for the first time a comparative analysis of blood and organ
system data from trials with rats fed three main commercialized genetically
modified (GM) maize (NK 603, MON 810, MON 863), which are present in food
and fee... 阅读全帖 |
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h****6
发帖数: 229 | 25 Can you synthesize the first 60 bp and make changes to the sequence to
change the GC/AT ratio for PCR? |
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c*****e
发帖数: 436 | 26 it seems the Chemist synthesized this peptide told me that in this peptide
valine or some thing? can be oxidized? (don't know what does this mean).
And I saw from papers other people make similar sample with very complicated
steps with:
"Samples were prepared for the NMR experiments in 6-mm tubes by dissolving 0
.3g of lyophilized material in 1.5ml of distilled H2O. ..The tubes were
placed in a bath at 20C, and argon gas was passed through the samples for 6h
by means of long-tipped pipets placed... 阅读全帖 |
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w******e
发帖数: 1187 | 27 想试试用aminoallyl-GMP在synthesize RNA的同时在5'加amine group，方便
下面做各种conjugation(with NHS ester of fluorophore/biotin)，不知有
什么注意事项没有？amine group应该很active 吧？用urea PAGE这种harsh的
条件purify会不会有问题？
多谢！ |
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a****o
发帖数: 1786 | 28 Is it possible to synthesize a short RNA oligo with NH2 at 5', anneal this
oligo to your single strand DNA template, add T7 RNA polymerase to
transcribe. The yield may be low, but the incorporation efficiency should be
really high. :))) |
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w******e
发帖数: 1187 | 29 I didn't know you can do that... so let me confirm: you mean, by anneal
a RNA oligo to a ssDNA template to make partial strand, the T7 polymerase
actually synthesize RNA by adding NTP to the existing RNA oligo, kinda
like a primer?
be |
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x********u
发帖数: 430 | 30 You can try alternative heating and cooling for breaking down the cell. 3X[
95C/1min and -80C/5min].
Try to use GoTaq Hot Start DNA polymerase.
If you still cannot get the desired band, try growing the cell in liquid
culture, and make genomic DNA by PureLink gDNA kit.
Since your fragment is around 200bp, why not just synthesize the sigle
strand oligoes and reconstitute your molecule by annealing on a PCR machine.
Good luck! |
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g*********d
发帖数: 233 | 31 Here is some literature I found....
I'm interested in this gene
Thanks!
2'-5'-oligoadenylate synthetase 1 is an enzyme that in humans is encoded
by the OAS1 gene.[1][2]
This gene encodes a member of the 2-5A synthetase family, essential
proteins involved in the innate immune response to viral infection. The
encoded protein is induced by interferons and uses adenosine triphosphate
in 2'-specific nucleotidyl transfer reactions to synthesize 2',5'-
oligoadenylates (2-5As). These molecules activate ... 阅读全帖 |
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j********r
发帖数: 156 | 32 Is there any disadvantage of using siRNA transfection for some functional
assay, such as cell viability or cell death? Can anyone recommend a vendor
for
synthesize dsRNA (if the target sequence is already known for efficient
knockdown, based on the use of shRNA)? Many thanks,
|
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w******e
发帖数: 1187 | 33 it's very easy to couple aptamers to sensor. in fact sensor field
is dominated by aptamer as affinity agent as the stability &
reusability is a big deal for sensors.
back to your questions, you can do thiol-gold, EDC-NHS, biotin-SA...
all you need is to synthesize the aptamer w/ desired functional group.
so are you doing arrays? or beads, like luminex? you may want to check
out larry gold's recent publication
I'd certainly like to, if it doesn't take too much time. any suggestions?
I'm confiden... 阅读全帖 |
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s********n
发帖数: 2939 | 34 If you know the sequences of them, just synthesize them, :) |
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M*****n
发帖数: 16729 | 35 even if they are 300bp 400bp it won't cost that much to have them
synthesized. |
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s********n
发帖数: 2939 | 36 Do you know where I could order these 300-400 bp oligos? I only find IDT
could synthesize <=200 bp. |
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a*****a
发帖数: 32 | 37 As you said, synthesize them, and mix together... |
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s********n
发帖数: 2939 | 38 I am not clear why you want to separate them since you already have the
synthesized ones, just curious, :) |
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a********k
发帖数: 2273 | 39 genscript can do that, less than $0.5 per bp for large gene synthesis. We
had a 6kb gene synthesized by them, correct clone. |
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m*p
发帖数: 226 | 40 Fibroblast are everywhere or in every organ, skin, heart, liver, etc.
Fibroblast are a type of mesenchymal cell, which are usually derived from
the epithelial-mesenchymal transition process. It seems fibroblast can be
derived from ectoderm, mesoderm, and endoderm. Fibroblast synthesize ECM and
collagen, and are important for organ structure (my personal two cents).
Myo-blast are myocyte progenitors. |
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a***e
发帖数: 1010 | 41 computer mutagenize, then chemical synthesize. |
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z*****6
发帖数: 1486 | 42 advantages of aptamer: longer shelf-life, higher stability, easy to
synthesize....
The most amazing fact of aptamer to me is, one can select aptamer against
almost any target! |
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w*****n
发帖数: 107 | 43 1. a) It is not surprising that a gene has multiple transcription start
sites.
b) You can use the Kozak sequence to PREDICT which ATG is likely to be
used as translational start site.
2). This looks odd.Are you sure the direction of the gene you look at is
correct? Because in the kit, there use random oligo to synthesize cDNA,
followed by gene-specific primer for PCR.
Good luck! |
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f****h
发帖数: 41 | 44 Dear all,
I would like to synthesize a bacteria gene in a codon-optimized version for
the expression in mouse neurons.
Can anyone share some experience regarding this service from different
companies, like IDT, DNA2.0,
GenScript? Thank you! |
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n********k
发帖数: 2818 | 45 As you folks are discussing Cori, I happened to read his autobiography and
find it both interesting and enlightening:)))...BTW, it sounds Richard Axel
is a lot nicer than what I/we have heard...or in another word, his cruelty
is rooted in his obsession with science and indifference to humanity:))); As
well, it is pretty...he noted Cori but left his ex out...pretty human or
not human?
Autobiography
Richard AxelNew York City is my world. I was born in Brooklyn, the first
child of immigrant parents... 阅读全帖 |
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a*****y
发帖数: 277 | 46 PCR should work but your primers will be expensive enough that you'd better
to get the gene chemically synthesized. 720 bp = 300 USD? |
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w******e
发帖数: 1187 | 47 请教：用cell-free expression synthesize peptide/protein, 要求newly synthe-
sized peptide/protein自动被immobilize到beads上，并达到尽量高的copy number。
最直接的方法大概是在gene里加个FLAG/HA的sequence，beads上coat FLAG/HA
antibody。但Ab个头太大担心copy number上不去。
请教有什么alternative method？比如his6-peptide的coverage会不会更高？
比如有没有site-specific biotinylation的reagent可以跟cell-free expression
合用？
非常感谢！ |
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n******u
发帖数: 418 | 48 i think it reads from 3-5
so the synthesized rna is 5-3 |
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l*****a
发帖数: 38403 | 49 【 以下文字转载自 TrustInJesus 讨论区 】
发信人: ainosunshine (mecca), 信区: TrustInJesus
标 题: Re: 再聊进化论：热运动组装第一个蛋白质
发信站: BBS 未名空间站 (Sat Sep 10 22:03:42 2011, 美东)
查了一下这个journal的impact factor只有2.3 http://www.springer.com/life+sciences/journal/11084
一般来讲生物学的普通质量的文章impact factor也是大于8的。像science,nature,都
是30以上的。如果只有这么低的impact factor，我觉得，这样的文章是不会被well
recognized.
从有机化学来讲我谈谈对作者所设计实验的看法。
"Here we show that RNA-like polymers can be synthesized non-
enzymatically from mononucleotides in lipid environments."
这个又说明什么问题呢？首先... 阅读全帖 |
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