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Z**R
发帖数: 1233 | 2 Nasa launches rocket successfully
The US space agency (Nasa) has launched a prototype rocket designed to
replace the ageing space shuttle.
The Ares I-X blasted off from Florida on a flight that will test technology
for the development of a future manned launch vehicle.
The 100m-tall, syringe-like rocket roared into the sky at 1530 GMT from Nasa
's Kennedy Space Center.
The stick-thin launcher is the first Nasa has built in more than three
decades, but its future is uncertain.
The $450m (300m eur |
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M****e
发帖数: 70 | 3 search pubmed for publications about diabetes research, tons
of them have done assays for serum insulin and ketone (butyrl
ketone). sera should be isolated using specific insulin syringe,
Sarstedt microvette to avoid lysis. VWR should have them.
the processing should be as smooth as possible. store sera
@ 4C O/N, spin @ 3000rpm 20min RT, transfer supernate. if red,
then byebye. the sample should be almost clear, a little yellow
or pink is fine. Crystal chem has ELISA kit. |
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p****e
发帖数: 105 | 4 发在这里，斑竹留情，材料都是生物版的同学们每天用的pipette, syringe filter,
tubes, O-ring。 |
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a**y
发帖数: 464 | 6 你这样作容易损坏pump。
虽然你是好心，不过效果很发指啊。
你先得用syringe排掉pump里面的气泡。不然pump很容易坏的。 |
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s******y
发帖数: 28562 | 7 $138 for 50 pieces is not expensive. What you can do is to borrow one from
some other lab.
They are NOT reusable.
For chloraform, you probably should try the nelon filter. However I suggest
you to call the technical support of that company or look up the product
datasheet to confirm.
The other thing you can try is pipet filter, but they are more expensive
than the syringe filter in my memory. |
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T**********t
发帖数: 1604 | 8 50ml的lysate直接过0.22um的filter的话，绝对是会堵的。我那时是用syringe拿手压
的，连换了三个filter才算搞定，累了个半死。如果用vacuum可能会容易一点吧。
centrifuge |
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c*****o
发帖数: 18 | 9 细菌的细胞培养液有抗菌活性（可能是一种细菌素），过了0.22um syringe filter灭
菌后，没活性了？百思不得其解，请教各位大侠 原因？小生谢谢先 |
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w******e
发帖数: 1187 | 10 我同学做过。直接打就行。需要点仪器（比如syringe pump之类）。
也可以考虑买点dharmacon/ambion的所谓self-delivering siRNA呵呵 |
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h**********r
发帖数: 671 | 11 同上另外一种方法。
Yeast “smash-and-grab” DNA prep:
• pipette 1-2 ml YPD onto transformation plate; scrape colonies off
with the end of a glass slide and pipette into a microfuge tube
• spin down 15 sec; pipette off sup; if cell pellet is more than 50-75
μl, remove excess and discard
• to cell pellet add 0.2 ml lysis buffer, 0.2 ml phenol/CHCl3 and 0.3
g 0.45-0.5 mm glass beads* (I use calibrated scoop made from cut microfuge
tube pierced with syringe needle) – seal carefully because be... 阅读全帖 |
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n***w
发帖数: 2405 | 12 嗯，有可能饱和了。
我昨天把1st binding后的supernatant又加beads了，看好没好些今天。
我觉得N-lau还是挺好使的。能solubilize大部分pellet。
我500ml的culture提了差不多160ug的蛋白。。。
我下次买点你说的B-Per试试好了～ （不过看成分也差不多）
我现在的protocol基本上是：(For 500ml culture)
1. thaw pellet on ice
2. add 18ml GST buffer (配方见上)，add 1% PI, add 2ml lysozyme (10mg/ml)，
mix on ice for 30 seconds
3. pass thru 20G syringe to reduce viscosity, also add DNAse 40ul (1mg/ml).
4. sonication (it will become clear)
5. 10000rpm, 40min，4deg
6. collect supernatant for GST beads binding
不过我这次还是将G... 阅读全帖 |
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L********g
发帖数: 4204 | 13 I am planning to do some protein purifications recently.
Is there any myc affinity column purchasable? I checked GE, there is no such
commercial column. Or people usually make their own myc column?
My lab has a FPLC system so that I really wish I can buy some column that I
can hook to the system (not a syringe of myc-matrix) .
I will giv Baozi to ppl who can help. Thanks. |
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b******r
发帖数: 111 | 14 Note: NHEs is membrane proteins that transmembrane 12 times. NHE5 is
localized to the plasma membrane;NHE6-9 reside on organellar membranes(
endosome,Golgi);
1. pcDNA transfects NHE5-9 through Fugene 6(ratio is 3 uL of Fugene/2 ug of
DNA) in 293 cells. After three days,collect cells.
2. Cold PBS washes cells. Add 150 uL of lysis buffer(final concentration:
50mM Tris pH7.4, 150mM NaCl, 1% triton, 0.1% sds, cocktail inhibitor)
into each well of 6-well plate. Scrape cells.
3. The lysate gets st... 阅读全帖 |
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r******y
发帖数: 21907 | 15 要订一些 syringe filters 有这么几种 不知道那种比较好？就是filter一些
solution而已，没有什么会造成腐蚀 |
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N2
发帖数: 81 | 16 you can use fast flow also, that will be fast.
use their pre-packed clumon and use a syringe if you only have small scale
sample. otherwise buy the 25 or 100ml resin and pack a column by yourself.
this resin has very good capacity. |
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b******y
发帖数: 627 | 17 For LZ,
sunnyday's desalting method is the best to try. I will be surprised if it
doesn't work. I guess you have to use a syringe because nobody likes to
inject 32P into their FPLC. In that case, you should be careful of the
fraction size. Don't mix two well separated peaks during fractionation.
kaka1000's vivapure spin column离子交换柱 is a cation/anion exchange column,
to which unlimited volume can be loaded. In the end, elute with B buffer.
The peak is sharp because the salt concentration is high ... 阅读全帖 |
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b******s
发帖数: 1089 | 19 非常感谢。我在公司主页看不到价格，能否告知Glass Syringe filters的价格？
Fi |
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r***e
发帖数: 2539 | 20 pellet里当然有病毒颗粒了，但是绝大多数是死细胞碎片，不知道你有没有离心两次，
第二次用20%蔗糖cushion，能改善一些，但是不多。
两次离心完了，我是用HBSS重悬后，用枪头吹100次，然后EP管10000rpm离心15秒，取上
清，把大多数碎片去掉。一般的hamilton syringe不会堵。
吗？ |
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l******n
发帖数: 143 | 21 提供点俺的意见供你参考哈,希望不要介意中英文掺杂,有的东西英文描述简单些习惯些
(1)很多物理过程和化学过程都有热效应,比如说protein oligomerization including
protein aggregation.简单说,如果有检测到的热效应,不代表一定来源于你希望研究的
那个特异性反应.需要先排除其他情况才能做出推断.在ITC实验里,有的蛋白或者其他分
子很不适应被高速搅拌,所以有可能产生沉淀,这个过程混合在其他过程中,比如有热效
应的特意反应中,可能造成没有明显规律的信号.简单的方法是ITC titration后取出
cell里的溶液看有没有明显沉淀,跑个UV-Vis spec,如果明显340-320 nm的吸收值比以
前高,有可能有大分子形成.具体一些的操作,可以先把两个反应中的分子以及其混合物(
两种分子比例尽量保证反应饱和)进行简单分析,跑柱子/胶/高速分析离心;在ITC实验后
,在cell里的混合物(应该主要是两种分子的聚合物,如果确定反应达到饱和的话)取出进
行同样手段分析,跑柱子/胶/高速分析离心.SEC解析度比较低,但有大分子形成也能很快
检测出.... 阅读全帖 |
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g*********5
发帖数: 2533 | 24 Isolation of nuclei for the co-IP and whole ChIP protocols is based on the
methods of Gendrel et al. (2002, 2005), Johnson et al. (2002), and Nelson et
al. (2006).
METHOD
For extraction and co-IP of nuclear proteins, see Steps 1-39 (Fig. 1). For
the ChIP procedure, see Steps 40-69 (Fig. 2).
Figure 1.
View larger version:
In this page
In a new window
Figure 1.
Flowchart for the timeline and organization for co-IP of nuclear proteins
from Arabidopsis seedlings.
Figure 2.
View larger versio... 阅读全帖 |
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h*j
发帖数: 77 | 25 needles BD 27G1/2
syringe 1ml |
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C****3
发帖数: 162 | 26 Head of Formulation development of Antibody drugs， if interested, please
contact
Vic Li, Cellphone: +86 151 2101 2330 eMail: [email protected]
/* */
-Preformulation, formulation development, and process development
activitiesthat supports the drug development and technical transfer
of clinical andcommercial antibody products.
-Development, transfer, process characterization, and validation of aseptic
vial,syringe and drug delivery devices.
-Lead and train the team to acc... 阅读全帖 |
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b**********s
发帖数: 410 | 27 感谢大家的input，非常有帮助。
能否再请教一下peristaltic pump, flow pump, syringe pump （这个就是“麻醉泵”
吗）的区别？
我们的实验只需要让液体从一侧流入，另一侧流出即可，所以实验探索阶段用注射器或
输液器注入确实也是一个可行办法。
我们准备测量液体注入之前和流出之后的电化学性质，是不是只能注入之前测量一次，
流出之后收集再测量一次？有没有更好的办法？
谢谢！ |
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d******e
发帖数: 58 | 28 俺们用的哈佛的
syringe pump, sci fisher is one choice |
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S*****n
发帖数: 6055 | 29 syringe pump一般承受压力不超过200psi吧？我看fisher上面是这样的。。。 |
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d******e
发帖数: 58 | 30 什么？我们最近order了一个好像是phd4400，绝对比你说的这个压力大多了
syringe pump一般承受压力不超过200psi吧？我看fisher上面是这样的。。。 |
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d******e
发帖数: 58 | 31 可能觉得耶鲁钱多，lol
不记得了，反正挺贵
找找Flow Rate Minimum 0.0001 l/hr
Average Linear Force 200 lbs (fluid pressure > 1,800 psi with an 8 ml
stainless steel syringe
比这个稍微差点的。另外要考虑syrange以后可能要更换，也是一笔钱。
这家就是很贵，但是真的需要也只能花这些钱了，反正我们是买了。
你那个link点进去是$4805.00。。。 |
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S*****n
发帖数: 6055 | 32 哦
那syringe pump不用controller吧？ |
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m****e
发帖数: 255 | 34 Just re-read op's post. It sounds like you are using syringe to inject. |
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x****o
发帖数: 60 | 35 Maybe you need a new syringe or plunger. The plunger is too loose, |
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a******3
发帖数: 159 | 36 想order那种很长的needle，可以连在syringe上，transfer liquid的。以前用过，记
得蓝色长盒子装的，但忘了是从哪个公司order的。现在的实验室在engineering,也没
人知道。请教各位chemists!
谢谢！！！ |
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j******n
发帖数: 1727 | 37 小弟正在做水氧化，不过遇到了一个GC进样的问题
我用的是号称gas tight syringe，结果每次进样总会见到氧气和氮气的峰
所以没法定氧气的量
觉得应该是手动进样的时候，针头里面有空气
有没有什么办法可以解决？
谢谢 |
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s****y
发帖数: 62 | 38 实验一直出不来稳定的结果，所以想在这里看看有没有人做同样的方向，能够给我一些
意见。
实验室里有一个5.5立方米的气袋，我的实验是：每次用syringe 吸取一定量的a-
pinene 或者是
limonene，大概是8uL或者是16uL，然后让干净的流动空气带着挥发的有机物进入气袋
。每次我大概让空
气充气袋40分钟-1个小时左右。（当然，提前先抽空一部分气袋，大概抽空20%左右）
。 这样气袋里面就充
满了带有样品的气体。当然这个时候，气袋是密封住的。所有的开口都关上。
然后我用tube连上泵，泵就以5L/min的速度从气袋里面抽空气流过tube。我就把SPME
fiber放在tube的
中间，然后让流过的样品空气，这样SPME就把样品吸附上去。
我的采样时间是2分钟。然后立刻用GCMS去分析样品。
采样条件
Experiment condition :
Temperature : room temperature
Flow rate : 5L/min
Sampling time : 2min
GC desorption time 5min @ 300℃
但是每次我的GC出来的峰面... 阅读全帖 |
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G****e
发帖数: 11198 | 39 Sangji was transferring up to two ounces of t-butyl lithium from one sealed
container to another when a plastic syringe came apart in her hands, spewing
a chemical compound that ignites when exposed to air.
竟然用塑料注射器！一个没有经过非常熟练培训过的学生，是不能用这种偷工减料的方
法来转移叔丁基锂。正丁基锂还ok，叔丁基锂是相当地危险。 |
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e*l
发帖数: 585 | 40 t-buli 用plastic syringe应该没问题吧，以前实验室一哥们，整瓶整瓶地用，不过这
小姑娘太胆大了，当时也没穿实验服。
sealed
spewing |
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l*****7
发帖数: 2844 | 41 塑料syringe不是都是一次性的吗？
from |
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q****i
发帖数: 6923 | 42 有比FIsher和VWR便宜的地方吗？能给给cataloguenumber吗？包子答谢。 |
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d********w
发帖数: 1110 | 43 前两天刚从系里的fisher store领了一个，看了一眼价钱，好像也就三四十块吧。这东
西再便宜能便宜到哪去？ |
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m********8
发帖数: 314 | 45 请教一般医用的聚合物注射器能耐多大压力？我想用气泵加压注射器进料，但不知可行
否。我尝试用力压一个出口被堵塞的35ml注射器（Kendall monoject Luer lock
syringe），注射器活塞倒是能承受得了。但我不知道我用的力量有多大。 |
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L****r
发帖数: 333 | 46 嘎tech化学 got Younan Xia, in 2011 he publsihed 11 Angewandte Chemie
International Edition.
(1) Silver nanocrystals with concave surfaces and their optical and surface-
enhanced Raman scattering properties
Xia, X.; Zeng, J.; McDearmon, B.; Zheng, Y.; Li, Q. and Xia, Y. Angewandte
Chemie International Edition 2011, 50, 12542-12546 (VIP article, highlighted
on the inside cover).
(2) Shape-controlled synthesis of copper nanocrystals in an aqueous solution
with glucose as a reducing agent and hexadecyl... 阅读全帖 |
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q****i
发帖数: 6923 | 49 ０。４５um的syringe filter过滤nanoparticle和polymer的混合物5ml。溶剂是水和乙
醇。现在在用PTFE的filter觉得太贵了没必要，想换个便宜的。求推荐。 |
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