M****e
发帖数: 70 | 1 make sure that you are aware of the buffer effect:
It is estimated that DNA has 15% lower absorbance in TE
buffer, 23% lower in TE+saline compared to water.
1 O.D.260 are 38, 45 and 50 ug/ml for DNA in ddH2O,
TE and TES, respectively. I assume the same for RNA,
and 1 O.D.260 are 40 ug/ml for RNA in TE (from Ambion).
btw, A260/A280 is different for pure RNA
DEPC'd H2O (pH5-6) 1.60
Nuclease free H2O (pH6-7) 1.85
TE (pH8.0) 2.14 |
|
X****i
发帖数: 492 | 2 【 以下文字转载自 Olympics 讨论区 】
发信人: XianDi (先帝), 信区: Olympics
标 题: Re: nature的原文可以找到么
发信站: BBS 未名空间站 (Fri Aug 3 09:30:00 2012, 美东)
找到了：
Why Great Olympic Feats Raise Suspicions: Scientific American
“Performance profiling” could help catch athletes who use banned performan
ce-enhancing drugs
From Nature magazine
At the Olympics, how fast is too fast? That question has dogged Chinese swim
mer Ye Shiwen after the 16-year-old shattered the world record in the women'
s 400-metre individual medley (400 IM... 阅读全帖 |
|
k******0
发帖数: 1073 | 3 It's désolé without the accents.
It means sorry.
It's used with Être (To be) to express that you're sorry.
IE:
Je suis désolé (I am sorry)
Tu es désolé (You are sorry) - Informal
Elle/Il/On est désolé (She/He/One is sorry)
Nous sommes désolé (We are sorry)
Vous êtes désolé (You are sorry) Plural / Formal
Elles/Ils sont désolé (They are sorry) |
|
m******g
发帖数: 467 | 4 基本上就是题目+bulletin point的形式吧，不要纠结我对paper的品味，呵呵。新年快
乐！
PNAS 13.12.03
推荐：
1. Reintroducing domesticated wild mice to sociality induces adaptive
transgenerational effects on MUP expression
promiscuous / monogamous-line
higher MUP (pheromone) expression in pros-line
Male become more attractive
Difference in Mup promoter methylation level
2. High-throughput DNA sequencing errors are reduced by orders of magnitude
using circle sequencing
Previously ~0.1-1x10^-2
Unique library preparation strategy
Copie... 阅读全帖 |
|
m******g
发帖数: 467 | 5 基本上就是题目+bulletin point的形式吧，不要纠结我对paper的品味，呵呵。新年快
乐！
PNAS 13.12.03
推荐：
1. Reintroducing domesticated wild mice to sociality induces adaptive
transgenerational effects on MUP expression
promiscuous / monogamous-line
higher MUP (pheromone) expression in pros-line
Male become more attractive
Difference in Mup promoter methylation level
2. High-throughput DNA sequencing errors are reduced by orders of magnitude
using circle sequencing
Previously ~0.1-1x10^-2
Unique library preparation strategy
Copie... 阅读全帖 |
|
w*****s
发帖数: 230 | 6 附件是主治大夫的reasoning(email回复，红字)， 他的水平我们还是很佩服的，因为
他已开始猜了个病就和我们宝宝症状非常一致，可惜后来发现不是；而且他不是代谢病
专家。
问题是坚持宝宝不吃长链脂肪酸的奶粉后，他奇迹般地改善了；完全出乎医生们的预料
。我感觉医生列的是常见的代谢病，而我们感觉宝宝吃长链脂肪酸似乎是慢性中毒，大
概需要一个月左右才能体现出来。因为过程很慢，我们怀疑很多方法查不出来。
我们最大的问题是如果是这种病怎么查？不能确认的话他一辈子吃饭都受影响，基本也
就很难出门时间长一点了。尤其是他的肝已经在要换的边缘。医生们现在改口说他的病
程都是偶然，可我们不能同意。为什么每次都是长链吃一段时间就得病，尤其是这次停
了鸡蛋后马上改善。。
谢谢大家。 （下面是文字，可惜设置不成红色）
Q: Our biggest concern is the reason of his liver damage.
A: The biopsy shows severe fibrotic changes and tells us that this is not a
liver that ... 阅读全帖 |
|
|
s********n
发帖数: 2939 | 8 现在这种例子越来越多了
http://www.nytimes.com/2014/06/05/health/in-first-quick-dna-tes
In a First, Test of DNA Finds Root of Illness
Joshua Osborn, 14, lay in a coma at American Family Children’s Hospital in
Madison, Wis. For weeks his brain had been swelling with fluid, and a
battery of tests had failed to reveal the cause.
The doctors told his parents, Clark and Julie, that they wanted to run one
more test with an experimental new technology. Scientists would search
Joshua’s cerebrospinal fluid for piece... 阅读全帖 |
|
t*s
发帖数: 1504 | 9 【 以下文字转载自 Faculty 讨论区 】
发信人: tes (286), 信区: Faculty
标 题: !!! How to adjust precision in CPLEX !!!
发信站: BBS 未名空间站 (Tue Oct 16 14:16:19 2007), 转信
Urgent!!!
How to adjust CPLEX so that it produces a less precise result (for less time) |
|
h*****2
发帖数: 991 | 10 可以问哪两个执照马？
中文的是不是secondary education +chinese endorsement?
因为我在IL endorsement requirement 里面看到这么一条
“Persons who have less than the 20 semester hours of content in a language
but who hold an early childhood, elementary, special or secondary
certificate can obtain an endorsement by passing the content-area test in
the specific language, or, if there is no content-area test provided by ISBE
, the appropriate language proficiency test listed under transitional
bilingual education. If there is no tes |
|
a*****g
发帖数: 19398 | 11 By Eric Schulzke , Deseret News National Edition
Published: Thursday, Jan. 22 2015 7:00 a.m. MST
While research shows the benefits, in-school and after-school programs del
iver the goods. (DimaSobko, Getty Images/iStockphoto)
The first time children called her "The Chess Lady" in a Seattle school 10 y
ears ago, Wendi Fischer was caught off guard. As she walked in, they started
buzzing, and when she asked the teacher what they were saying, it turned ou
t she was a minor celebrity. The children ... 阅读全帖 |
|
d*******l
发帖数: 110 | 12 站内发简历，我会转给那个经理。
》》》》》》》》》》》》》》》
Located at the heart of Telecom Corridor in Richardson, Texas, a fast
growing wireless communication startup is looking for a hardware test intern.
· RESPONSIBILITIES:
· Assemble and test prototype electronic and rf assemblies
· Design wiring harnesses and system cabling
· Check schematic netlists and BOMs
· Coordinate fabrication and turn-key assembly of PCBs with vendors
· Set up and maintain electronics lab
· Design and build test fixtures
· Define tes |
|
l**********i
发帖数: 7 | 13 公司在上海徐汇区，标准美资企业，大型Fabless公司,去年效益非常好，共招4位（1+2
+1），
本来很多职位，都快招完了：）。薪水不错。而且公司今年配股了。来试试吧！！
请发送简历到l************[email protected]
因为申请人数会比较多，所以漫天要价不现实。合理的薪水，适当的涨幅能提高录用率
（跟自
己水平也相关），漫天要价则hiring manager直接忽略。
具体的岗位要求没有太多特殊的东西，但是需要英语好，因为要经常跟老美沟通，写
email，开
电话会议。
Test Engineer(2)
- To support prototype and production releases of new products. Manage debug
of test
program and hardware and meet product release schedules.
- Support test program development for implementing changes in new test
methodologies
for tes |
|
g*********i
发帖数: 281 | 14 【 以下文字转载自 ChinaNews 讨论区 】
发信人: DarthSidious (Sith Lord), 信区: ChinaNews
标 题: Re: 就最近的验血的争论，说两句
发信站: BBS 未名空间站 (Mon Dec 3 22:14:43 2007)
The statutory provisions you mentioned are all about drug or alcohol test. I
seriously doubt that any state government could discriminate against AIDS
patients, or any kind of patient.
Government drawing a line based on health condition is very unlikely to
prevail against a constitutional challenge.
The supreme court will probably invoke the strict scrutiny tes |
|
L*****r
发帖数: 722 | 15 不客气。如果您也在考版，就更有共同语言了。
术后半年，降钙素翻番，而且绝对值高于100 pg/ml，我有点不放心。查了些资料，关
于降钙素绝对值的意义的，只找到两篇：
An Italian study of 5817 patients with thyroid nodules found nine patients
with calcitonin >100 pg/ml, all with medullary cancer (0.15 percent); eight
patients with calcitonin between 50 to 100 pg/ml (100 ng/L), four of whom
had positive pentagastrin tests, and two of whom had medullary cancer (0.03
percent); 49 patients with calcitonin between 20 to 50 pg/ml (20-50 ng/L),
12 of whom had positive pentagastrin tes |
|
c*******s
发帖数: 399 | 16 they are very professional, and very into the role playing. Somethose are
warm,some are flat,and the others are cool just like people you will
encounter in the real world. I did not have any angry sp but the other row
had one.
not too many drugs mentioned, about two, can't disclose the names, but, yes
, i knew the name they talked, they are common drugs.
my guess is practice, practice, and practice. Kaplan 5 days course might be
a good try, I have no experience though.
good luck with your tes |
|
l*y
发帖数: 21010 | 17 而智商几乎全部取决于遗传。建议尽早去约心理医生测个IQ，测完了，心里有数，也就
踏实了，不瞎折腾了。
我可不是信口胡诌，我有参考文献！
Linda Gottfredson, “Logical Fallacies Used to Dismiss the Evidence on Intel
ligence Testing,” in The True Measure of Educational and Psychological Tes
t s: Correcting Fallacies about the Science of Testing, edited by R. Phelps
(W ashington, D.C.: American Psychological Association, in press).
认知测试学界公认的是，Despite widespread condemnation of standardized tests,
the data are unambiguous that they are the best available predictor of a... 阅读全帖 |
|
l*y
发帖数: 21010 | 18 大家帮我看看我引的这个文献是否准确
Intel
Tes
Phelps
tests,
acad |
|
l**********o
发帖数: 2758 | 19 有道理。
Intel
Tes
Phelps
tests,
acad |
|
y**s
发帖数: 563 | 20 情绪智商不只是关系到一个人的身心健康，而且对事业的成功亦至关重要。最近
研究统计，一个人的成功只有２０％归诸于ＩＱ（智商）的高低，８０％取决于ＥＱ
（情商）。ＥＱ高的人生活比较快乐，能维持积极的人生观，不管做什么事成功的机会
都比较大。情商（ＥｍｏｔｉｏｎａｌＱｕｏｔｉｅｎｔ简称情感智商）是由美国心理
学家丹尼尔·戈尔曼提出的，他认为：利用智力测验或标准化的成就测验来衡量一个人
的智力，并预测其未来的成败，实际上比不上利用情绪的特质来衡量它更具有意义。
Intel
Tes
Phelps
tests,
acad |
|
t**********d
发帖数: 638 | 21 基本同意。娃生下来，体质，智力，容貌等等，八九成就定了。
问题是百分之九十plus 的人群都是均数范围内。
Outliers: The Story of Success， 作者Malcolm_Gladwell在
书里反复说的10 thousand hours rule，就是＂推＂的指导思想吧。
Intel
Tes
Phelps
tests,
acad |
|
l*y
发帖数: 21010 | 22 而智商几乎全部取决于遗传。建议尽早去约心理医生测个IQ，测完了，心里有数，也就
踏实了，不瞎折腾了。
我可不是信口胡诌，我有参考文献！
Linda Gottfredson, “Logical Fallacies Used to Dismiss the Evidence on Intel
ligence Testing,” in The True Measure of Educational and Psychological Tes
t s: Correcting Fallacies about the Science of Testing, edited by R. Phelps
(W ashington, D.C.: American Psychological Association, in press).
认知测试学界公认的是，Despite widespread condemnation of standardized tests,
the data are unambiguous that they are the best available predictor of a... 阅读全帖 |
|
l*y
发帖数: 21010 | 23 大家帮我看看我引的这个文献是否准确
Intel
Tes
Phelps
tests,
acad |
|
l**********o
发帖数: 2758 | 24 有道理。
Intel
Tes
Phelps
tests,
acad |
|
y**s
发帖数: 563 | 25 情绪智商不只是关系到一个人的身心健康，而且对事业的成功亦至关重要。最近
研究统计，一个人的成功只有２０％归诸于ＩＱ（智商）的高低，８０％取决于ＥＱ
（情商）。ＥＱ高的人生活比较快乐，能维持积极的人生观，不管做什么事成功的机会
都比较大。情商（ＥｍｏｔｉｏｎａｌＱｕｏｔｉｅｎｔ简称情感智商）是由美国心理
学家丹尼尔·戈尔曼提出的，他认为：利用智力测验或标准化的成就测验来衡量一个人
的智力，并预测其未来的成败，实际上比不上利用情绪的特质来衡量它更具有意义。
Intel
Tes
Phelps
tests,
acad |
|
t**********d
发帖数: 638 | 26 基本同意。娃生下来，体质，智力，容貌等等，八九成就定了。
问题是百分之九十plus 的人群都是均数范围内。
Outliers: The Story of Success， 作者Malcolm_Gladwell在
书里反复说的10 thousand hours rule，就是＂推＂的指导思想吧。
Intel
Tes
Phelps
tests,
acad |
|
p********y
发帖数: 146 | 27 听上去很有道理，不过类似的“公认”不可通过研究来重复。你总得不到你期望得到的。
Intel
Tes
Phelps
tests,
acad |
|
B*D
发帖数: 5016 | 28 http://goo.gl/TXhT6E
New Yorker的专栏
HOW DO HEDGE FUNDS GET AWAY WITH IT? EIGHT THEORIES
The other day, I asked how hedge funds manage to bestow such great riches on
their managers despite the fact that, in many cases, their performance seem
s pretty ordinary. That got quite a reaction. The responses ranged from clai
ms that hedgies are remunerated perfectly appropriately to charges that they
are outright crooks who prey on gullible and greedy investors. Because the
industry has grown enormously i... 阅读全帖 |
|
p********a
发帖数: 5352 | 29 ☆─────────────────────────────────────☆
mrdi (不穿鞋的刀客终日乾乾，夕惕若，厉无咎。) 于 (Fri Jul 22 18:21:49 2005) 提到:
why do you still care about grades.
you do want to learn something, right?
btw, it is not true that all american students are bad,
some of them are super cow too
☆─────────────────────────────────────☆
tes (286) 于 (Fri Jul 22 19:13:10 2005) 提到:
我身边的美国同学天才的或许很少，但一般都非常努力，比我努力多了
经常晚上11点还看到他们在系里讨论题目。。。
☆─────────────────────────────────────☆
sampdoria (samp) 于 (Fri Jul 22 19:25:14 2005) |
|
q**j
发帖数: 10612 | 30 just as i said. put ?? in front of the informat for the variable that genera
tes the error message. |
|
p********s
发帖数: 36 | 31 最近在找工作，和recruiter talk了以后，他给我发了一个在proveit.com上的SAS
test, 有没有童鞋take过kenexa "prove it" 上的SAS test。想问一下这种test难不难
， 都会问些啥。 说是不需要用sas 做这个test. 是不是和base 和advanced
certification 差不多呀。 先拜谢了！！！ |
|
p********s
发帖数: 36 | 32 最近在找工作，和recruiter talk了以后，他给我发了一个在proveit.com上的SAS
test, 有没有童鞋take过kenexa "prove it" 上的SAS test。想问一下这种test难不难
， 都会问些啥。 说是不需要用sas 做这个test. 是不是和base 和advanced
certification 差不多呀。 先拜谢了！！！ |
|
|
d*********g
发帖数: 349 | 34 我刚好也被发了个这个考试
请问有没有大侠们做过？说下经验贝。 比sas adv考试怎样，难很多么？
谢谢！！！ |
|
t********y
发帖数: 469 | 35 申请人数已经满了20了
申请李宇春俱乐部
希望各位支持，英文：ChrisLee
希望站方和广大id支持，谢谢。
截至目前为止，已经有
1. lingling7
2. mushroom
3. oldragon
4. poonie
5. derek
6. telen
7. frona
8. winterflower
9. ppaitt
10.shuanna
11.happykitty
12.zhizunbao
13.magnolia
14.tes
15.yumiyumi
16.lhmaymay
17.xiaoenda
18.xiongxiongmao
19.cozy
20.EverestInn
21. redfisher
22.bobococo
23.varlet
24.Firstep
网友支持。呵呵 |
|
t********y
发帖数: 469 | 36 1. lingling7
2. mushroom
3. oldragon
4. poonie
5. derek
6. telen
7. frona
8. winterflower
9. ppaitt
10.shuanna
11.happykitty
12.zhizunbao
13.magnolia
14.tes
15.yumiyumi
16.lhmaymay
17.xiaoenda
18.xiongxiongmao
19.cozy
20.EverestInn
21. redfisher
22.bobococo
23.varlet
24.Firstep
25.givemefive
26.bim
27.sai
28.turnanyway
29.happypanda
30.north
31.ChinaGo
32.scarletleaf
33.feir
34. wenny2010
35。fyne
36.betta
37.smaragdos
38.Gypsygirl
39.wakeupgogo
40。fatblackliar
41。ikea
42.mudd
43.skyskylei
4 |
|
a********s
发帖数: 557 | 37 大家可以看看it封国几个菌斑满嘴喷粪的小将。
-----------------------------
版内文章搜索列表
查找讨论区'Notice'内， 标题含：'Military'和'roseriver',不含'解封' 作者为：'所
有者'，'7111'天以内的文章
序号 标记 作者 日期 标题
1 * deliver Oct 16 roseriver 封 goodegg02 在 Military 版
2 * deliver Oct 16 roseriver 封 weiqigogo 在 Military 版
3 * deliver Oct 16 roseriver 封 thorton 在 Military 版
4 * deliver Oct 17 roseriver 封 fordate 在 Military 版
5 * deliver Oct 17 r... 阅读全帖 |
|
w*****s
发帖数: 230 | 38 【 以下文字转载自 Biology 讨论区 】
发信人: wwwjobs (minimouse), 信区: Biology
标 题: 请看看附件图片文字，建议我们能做什么呢？
发信站: BBS 未名空间站 (Thu Jan 16 12:24:03 2014, 美东)
附件是主治大夫的reasoning(email回复，红字)， 他的水平我们还是很佩服的，因为
他已开始猜了个病就和我们宝宝症状非常一致，可惜后来发现不是；而且他不是代谢病
专家。
问题是坚持宝宝不吃长链脂肪酸的奶粉后，他奇迹般地改善了；完全出乎医生们的预料
。我感觉医生列的是常见的代谢病，而我们感觉宝宝吃长链脂肪酸似乎是慢性中毒，大
概需要一个月左右才能体现出来。因为过程很慢，我们怀疑很多方法查不出来。
我们最大的问题是如果是这种病怎么查？不能确认的话他一辈子吃饭都受影响，基本也
就很难出门时间长一点了。尤其是他的肝已经在要换的边缘。医生们现在改口说他的病
程都是偶然，可我们不能同意。为什么每次都是长链吃一段时间就得病，尤其是这次停
了鸡蛋后马上改善。。
谢谢大家。 （下面是文字，可惜设置不成红色）
Q: Our big... 阅读全帖 |
|
|
f*********g
发帖数: 958 | 40 作者：魑魅魍魉姬 回复日期：2006-7-17 17:49:49
番茄小丸子~~~~
[雨停下]的介绍如下:
原唱下载: http://www.vscn.cn/0files/music/Cesse_la_pluie.mp3
歌词:
Là-haut sur un nuage
J’aimais sans me douter
Qu’éclaterait l’orage
Je n’ai pas vu le temps changer
Tes mots sur leur passage
Ont tout noyé, brisé
Tu es resté fermé
Je ne sais où aller...
Oublier cet orage éphémère
L’effacer et t’aimer comme hier
Je cherche et je cherche
Le remède pour qu’enfin
Cesse la pluie
Cesse la pluie
Oublier cet orage éphémère
L’effacer, r |
|
p*********e
发帖数: 32207 | 41 【 以下文字转载自 Automobile 讨论区 】
发信人: pandamalone (0000~史前圣灵猫熊), 信区: Automobile
标 题: zz:MIDSIZE LUXURY/NEAR LUXURY CARS FRONTAL OFFSET CRASH TEST DETAILS
发信站: BBS 未名空间站 (Thu Mar 22 18:25:55 2007)
ast. |
|
|
p**q
发帖数: 1130 | 43 check engine 灯亮，上周车检，emission test没过，catalytic converter寿终正寝了，换一套价格不菲，
想起以前在网上看到的小trick。
直接上图说话（光顾着干活了，没拍照，图片是网上找的）
1，在Oreilly买non-fouler，又叫anti-fouler，就是一小套筒，不知道中文名叫什么
2，将non-fouler扩孔，用的是1/2"的钻头
3，拆下downstream的o2 sensor，拧进non-fouler里
4，将套上non-fouler的o2 sensor装回原位
这样o2 sensor就不会直接暴露在气流中，不会检测到高浓度氧气。开着车跑了两天，
check engine 灯就自己灭了。再去修车铺，顺利通过smog test。 |
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h****y
发帖数: 9234 | 44 我就是这么应付我的引擎灯的,不过我们这没年检,把那灯弄灭纯粹是为了心里舒服以及
保证引擎灯能有警示效果.
寝了，换一套价格不菲， |
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