P*****l 发帖数: 438 | 1 How many people in this article are in trouble now ?
Tiny transistor breaks new limits
Thursday, 18 October, 2001, 14:03 GMT 15:03 UK
Bell Labs also built the first ever transistor in 1947
Scientists in the US may have paved the way towards working molecular
computers.
A group of researchers at Bell Labs have made tiny functioning transistors a
million times smaller than a grain of sand.
Making large quantities of the tiny components should be straightforward
because they self-assemble.
But rese... 阅读全帖 |
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l**i 发帖数: 416 | 2 T. Ito "Ion-Channel-Mimetic Sensor for Trivalent Cations Based on Self-
Assembled Monolayers of Thiol-Derivatized 4-Acyl-5-Pyrazolones on Gold" J.
Electroanal. Chem. 2001, 495, 87-97 |
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k*****m 发帖数: 5 | 3 Postdoctoral opportunity at UCSD:
I am helping my former mentor look for a postdoc. Please send your application directly to Professor Naviaux.
Here's the job posting:
The Naviaux Lab at the University of California, San Diego has an immediate
opening for a postdoc (MD, PhD, or both) with broad experience in the mass
spectrometry of small molecules and metabolomics. Specific experience with
the quantitative analysis of organic acids, amino acids, nucleotides, thiol
compounds, neurotransmitters, ... 阅读全帖 |
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p*****r 发帖数: 805 | 4 就象3-Aminopropyltriethoxysilane可以修饰氧化物表面然后同时另一段是amine
group一样。有没有用于修饰金的表面的类似的分子?希望在金表面利用NH-来接COO-的大分子。在哪里可以买到?谢谢~ |
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f*******e 发帖数: 628 | 5 看看那种卖 crosslinker 的公司,比如 Pierce 之类。
的大分子。在哪里可以买到?谢谢~ |
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f*****n 发帖数: 93 | 6 小分子 cysteamine, 高分子 HS-PEG-NH2 都可以吧, 但是这两种分子都会在某种程
度上导致颗粒聚集。
你要是用 -COOH 和 -NH2 的coupling反应,只能是在颗粒上修饰 -COOH, 然后再连 -
NH2 group 的分子, 研究一下反应机理你就知道怎么回事了。
的大分子。在哪里可以买到?谢谢~ |
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l**j 发帖数: 651 | 7 HS-(PEG)n-NH2 我曾经找过 似乎没有commercially available的
不知道是不是分子本身不稳定
- |
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f*****n 发帖数: 93 | 9 关键词 PEG products, google |
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l**j 发帖数: 651 | 11 我笨 当年Google了没Google到 刚刚又Google了 还是没Google到
楼上给的那个 是CH2 也不是peg |
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C*******b 发帖数: 174 | 14 一个非端基双键,两边连的都是烷基,其中一个双键氢被氯原子取代了。请问有没有
什么化合物或者反应能够在室温下高效与这样的双键发生反应的?谢谢了。注:thiol-
ene不行,效率很低。 |
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p**********g 发帖数: 378 | 16 Heck coupling....
thiol- |
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s*****0 发帖数: 244 | 17 Thiol–ene “click” reactions and recent applications in polymer and
materials synthesis: a first update
Andrew B. Lowe
Polym. Chem., 2014,5, 4820-4870
DOI: 10.1039/C4PY00339J
http://pubs.rsc.org/en/content/articlelanding/2014/py/c4py00339
email: [email protected]
(function(){try{var s,a,i,j,r,c,l,b=document.getElementsByTagName("script");l=b[b.length-1].previousSibling;a=l.getAttribute('data-cfemail');if(a){s='';r=parseInt(a.substr(0,2),16);for(j=2;a.length-j;j+=2){c=parseInt(a.substr(j,2),16)^r... 阅读全帖 |
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y****6 发帖数: 196 | 18 我需要标记一段50 nucleotide 的oligo 到抗体上,不知道什么方法比较合适?一个方
法是用pierce 的SMCC, 一端连抗体的amino group, 一端连thiol modified oligo;另
外一个方法是click reaction。用DBCO NHS 连上抗体,和Azimde modified oligo 反
应。不知道哪种方法效率高些?
还有就是纯化,dialysis 好像找不到合适的MWCO。有什么好建议吗?oligo 大约15kD
,抗体150kD。
★ 发自iPhone App: ChineseWeb 1.0.1 |
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q****i 发帖数: 6923 | 19 Microporous and Mesoporous Materials
Volume 115, Issue 3, 1 November 2008, Pages 383–388
Synthesis and characterization of hollow zeolite microspheres with a
mesoporous shell by O/W/O emulsion and vapor-phase transport method
http://www.sciencedirect.com/science/article/pii/S1387181108000
Concentric Hollow Nanospheres of Mesoporous Silica Shell-Titania Core from
Combined Inorganic and Polymer Syntheses
Guoliang Li, E. T. Kang, K. G. Neoh and Xinlin Yang
Langmuir, 2009, 25 (8), pp 4361–4364
Publi... 阅读全帖 |
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i*n 发帖数: 9793 | 21 A fluorescent probe capable of discriminately and simultaneously detecting
dl-dithiothreitol and single sulfhydryl-containing thiols
Sihua Guo 1, Fangyuan Zheng 1, Fang Zeng, Shuizhu Wu
Sensors and Actuators B: Chemical
Volume 224, 1 March 2016, Pages 88-94
https://doi.org/10.1016/j.snb.2015.10.036
Thanks! |
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i*n 发帖数: 9793 | 22 A fluorescent probe capable of discriminately and simultaneously detecting
dl-dithiothreitol and single sulfhydryl-containing thiols
Sihua Guo 1, Fangyuan Zheng 1, Fang Zeng, Shuizhu Wu
Sensors and Actuators B: Chemical
Volume 224, 1 March 2016, Pages 88-94
https://doi.org/10.1016/j.snb.2015.10.036
Thanks! |
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s**********e 发帖数: 2888 | 23 关于Allium类植物(葱,蒜)等等的营养学,有超级多的文献,包括非常多的流行病学
,统计学数据,算是研究比较充分的。
但是,所有的统计学数据都会告诉你,只有长期的大剂量的食用allium类植物,比如每
天或者每周都吃好几次,连续很多年,才会观察到对于某些疾病的效果。
我自己不太相信single dose的食用,会对疾病有效果,否则,如果有这么强的活性,
我们吃这些蔬菜岂不是胆战心惊?也许对于某些疾病,比如anti-bacterial, 我相信也
许会。
Allium类植物的活性主要是来自于其中的硫化物,thiosulfinates,他们的半衰期非常
的快,也许吃下去几分钟,绝大部分都会被代谢掉,转化为呼吸里面的thiol
compounds, 这就是为啥他们气味很大的原因。 |
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x*o 发帖数: 1037 | 24 Self-Assembly and Mineralization of Peptide-Amphiphile Nanofibers
西北的Stupp
Science, November 2001, 294, 1684
他们所合成的PA有三点features:
一, 为了增强fiber的强度,在peptide-amphiphile(PA)中引进了四个cysteine aa.在氧
化态下cysteine的thiol在fiber的表面形成双硫键,从而增强fiber的强度
二, 在PA中引进phosphoserine residue, 使hydroxyapatite(HA)更容易在fiber上成核。
三, 在PA中引入Arg-Gly-Asp以提高表面对细胞的附着和生长。
感觉有两个interesting的地方:
1, 可以用pH值控制PA可逆地self-assemble形成fiber
2, 在self-assembly以后,进行mineralize, 得到的HA的c轴与fiber的长轴方向相同。此
性质与“is the same as that observed between collage |
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c*s 发帖数: 2145 | 25 作 者 分 别 是 H. Weller 和 A.P. Alivisatos, 这 两 位 就 不 多 说 了
都 是 运 用 了 量 子 点 的 发 光 性 质 , 把 它 用 作 MARKER,
一 个 是 把 它 ENCAPSULATE进 MICROSPHERE里 , 这 里 面 用 了 POLYELECTROLYTE
和 量 子 点 的 静 电 吸 引 作 用 , 把 量 子 点 固 定 在 microshere里 。 有
点 意 思 的 是 两 点 , 1。 量 子 点 的 表 面 电 性 可 以 用 不 同 的
STABILIZER控 制 , 也 就 是 不 同 的 THIOL分 子 控 制 2。 通 过 控 制 量 子
点 的 大 小 ,控 制 其 发 光 位 置 , 由 于 不 同 颜 色 的 量 子 点 可 以 由
单 一 激 发 波 长 激 发 , 所 以 用 CONFOCAL显 微 镜 可 以 看 到 不 同 颜 色
的 MICROSPHERES, 这 个 过 程 就 是 LABEL的 过 程 , 可 以 用 于 昨 个 什 么
LIBRAR |
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c*s 发帖数: 2145 | 26 it depends on what nano you want and their quality.
For metal NPs, I think it is pretty cheap.
For II-VI semiconductors, it depends on the synthesis method.
For example, by thiol molecules as stibilizer for CdTe, it cost only ~1/10 as
the cost by TOPO/metal organic precursors.
I would say, you can make a big money if you can produce inductrial scale,
high quality semiconductors. |
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c*s 发帖数: 2145 | 27 I see what you meant.
I suggest you read Warren chan's paper in science 1998
the basic structure is NP was capped by amine thiol, which is futher linked to
carboxyl modified protein
there are two relatively new papers below:
especially in one paper, the QDs were used "in vitro" and in the other paper,
different colored QDs were selectively labelled in the core and surface of
cancer cell.
or you can refer to Mirkin;s papers, even though he is not in the area of
bio-dot
on this topic:
1、Immunofluo |
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t***u 发帖数: 20182 | 28 click chemistry.看看人家是怎么做的,azide alkye或者最近的thiol-ene |
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t***u 发帖数: 20182 | 29 click chemistry-thiol ene |
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m*****e 发帖数: 1506 | 30 chain-end thiol-ene就是一个joke,连trithiolcarbonate的aminolysis都是半个joke
,看着这类文章像泡一样冒个不停真觉得可笑啊。。。
ATRP chain-end functionalization绝对不比RAFT好多少,我觉得除了阴聚活性端功能
化有>95%其他都很难上90%。 |
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D********g 发帖数: 533 | 31 如果你功能团无所谓,那么就有很多反应效率很高的组合,比如
1. epoxy / amine
2. thiol / alkene
3. -COCl / -OH
4. Azide / Alkyne
等等 |
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c***r 发帖数: 4631 | 32 先谢谢各位师傅师哥师姐师弟师妹。
polymer source有几个,但是分子量都太小,最大的才16k,而且太贵。谁知道在哪儿
能买到Mn>100k,而且比较便宜的啊?
散尽家财发包子。 |
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I*****8 发帖数: 42 | 33 不能自己做吗?RAFT聚合后端基还原一下就行了 |
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a*i 发帖数: 1652 | 35 分子量越大,端基功能化就越不容易。自己做最靠谱。 |
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m******l 发帖数: 31 | 37 为什么thiol(-SH)很reactive,可以修饰 金,银,各种硫化物等
而同族的hydroxyl(-OH)却不可以? |
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C********5 发帖数: 194 | 38 Where did the H go when a thiol (-SH) reacts with gold? |
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f*********e 发帖数: 1144 | 39
drug
----Thanks a lot for the comments.
Right, Cys conjugation is one of the major types, either through partial
reduction or using an engineered Cys like you mentioned. If we really want
to pick some flaws, is there anything bad from the engineering? like
inconsistent scale-up? Also, how does the thiol group linked to melamide
behave in vivo? I once went to a siminar by someone from genentech talking about pk/pd, looks like there is still some amount of free drugs in serum. |
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E***n 发帖数: 308 | 40 Free drug may come from several sources, residues from incomplete
purification, or reversable linkage chemistry. There are always trace free
thiol. |
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c*s 发帖数: 2145 | 41 to seperate thiol capped nanoparticles, I think you can use
several methods.
1. add some solvents to make phase seperation, commonly used
solvents such as methanol, acetone or iso-propanol, this
method usually works, if th esolution is rotary evaporate
first, th eeffect is better
2. you can dialysis th esolution first to remove some
by-product, such as salts(supposed there exists some salts
in th esolution) after that, the particle became instable
and precipitate occurs, then you can use centrig |
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