W*******s 发帖数: 18705 | 1 Trolling Motor Mount Transducer每个厂家都有,买的是船尾,邮寄回去换一个就是
不要钱。7寸的声纳挺大的了,放卡娅或是小船上比较别扭。TM电瓶一个够了,买最大
容量的Deep Cycle,玩一天没问题,要注意的是起风了,你是顺流回来还是逆流回来,
这个能量消耗差别很大。 |
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u******w 发帖数: 1882 | 3 这样看来,船头trolling motor和船尾transom的transducer形状确实不一样。
另外我还想再问一下,fishfinder显示屏的大小除了耗电不同,有啥不一样?或者说我
买fishfinder最重要看哪些参数? |
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e***d 发帖数: 288 | 5
Hobie的话可以装在内部, 但是也是接触水的. |
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W**********d 发帖数: 94 | 6 一种装外面,一种装船内。
装船内的叫Shooting Though Hull。海船上是主流装法。
你卡娅里面可以隔水装船体内,有几个地方你要注意,第一,3D声纳,Side Image之类
的效果就没有了,只有2D声纳的效果。温度就是船壳内的温度,跟实际水温有点误差。
在装的时候要用粘料固定探头,不能有任何气泡,声纳的波段是穿不过空气的。通常用
橡皮泥先试试看哪个位置合适再用固定粘料。缺点是,装上之后就不能移动了,硬要移
动拆除会留有残渣印记。 |
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v*********w 发帖数: 1632 | 8 outboard在家里时可以放在架子上,在去钓鱼的路上还是得躺着放啊,有什么解决办法
吗?
另外,您的fish finder/transducer是怎么装的?有图可以借鉴一下吗?
谢谢 |
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P***5 发帖数: 1089 | 9 我用zip tie把transducer套在电马达桨叶前面一点, fish finder固定在木板上,木板
用松紧带套在座椅上。也可以试试粘在船底, 像kayak那样。
用的什么trailer?
: 我也是用一个小trailer拉着。porta bote一直放在上面。
: 你有装fish finder吗?
: 库,
|
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u******w 发帖数: 1882 | 10 短时间应该问题不大吧
: outboard在家里时可以放在架子上,在去钓鱼的路上还是得躺着放啊,有什么解
决办法
: 吗?
: 另外,您的fish finder/transducer是怎么装的?有图可以借鉴一下吗?
: 谢谢
|
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u******w 发帖数: 1882 | 11 那个fish finder我是用磁铁装上的,非常方便,几秒钟搞定。你可以搜一下
fishfindermounts,那个网站有很多照片和视频。
: outboard在家里时可以放在架子上,在去钓鱼的路上还是得躺着放啊,有什么解
决办法
: 吗?
: 另外,您的fish finder/transducer是怎么装的?有图可以借鉴一下吗?
: 谢谢
|
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m****s 发帖数: 10867 | 13 Commercial ammunition has a SAAMI/ANSI maximum pressure of 62,000PSI. While
not every manufacturer may load it to this level, this is the industry
established maximum. This is also measured using the piezo transducer method
. The proof cartridge pressure is 83,000 to 89,000 PSI. Note the differences
between the military test and operational standard vs the commercial one.
The worst case scenario for shooters of 7.62 NATO rifles is as follows:
Using a commercially made, maximum pressure cartridge |
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d******0 发帖数: 22800 | 14 【 以下文字转载自 HiFi 讨论区 】
发信人: dash2000 (DASH), 信区: HiFi
标 题: Phiaton PS200 Earphones - $94.49
发信站: BBS 未名空间站 (Tue Jul 31 00:40:33 2012, 美东)
on amazon.
http://www.amazon.com/Phiaton-PS-200-Transducers-andTweeter/dp/
I have and love it, and paid for $129 for it.
$95 is way too good to pass! esp in amazon, you could return if you don't
want, risk free. so... I ordered another 2 pairs. |
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f********g 发帖数: 706 | 15 I am experiencing an interesting thing. I went to bed around 11PM, but I
couldn't sleep. The reason is that there is an electronics movie flowing in
my minds. I don’t know why my neuron networks work in this way. Yes, on the
bed, I had an exciting mental journal about applied physics and I would
like to share this dream with you. There are two lines in my dream: the
dancing electrons and the quit information. Most of time, we are focusing on
building the tools which are derived from the dancing ... 阅读全帖 |
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z**x 发帖数: 3629 | 16 买这个吧,便宜量又足,还是USB 电容麦。
http://www.amazon.com/Blue-Microphones-Snowflake-USB-Microphone
Blue Microphones Snowflake USB Microphone
Price: $39.95 & FREE Shipping. Details
Amazon.com
Featuring Blue Microphones' renowned circuitry, the Snowflake is a
professional quality portable mic that combines a USB bus powered capsule
with a class compliant design to make the Snowflake truly plug and play on
both Mac and PC. Not just an eye-catching design, the Snowflake is highly
functional. The mic folds in... 阅读全帖 |
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f****p 发帖数: 18483 | 17
http://bbs.tianya.cn/post-funinfo-4023670-12.shtml
摘录如下。可惜了,好歹也算当过我徒弟了。。。
附录2:吐了大妈的简历
Haiying Zhou's Education
postdoc at University of California at Berkeley
San Francisco Bay Area
Research
Haiying Zhou's Education
The University of Texas Health Science Center at San Antonio
Ph.D., Molecular Medicine
2001 – 2008
Nankai University
B.S, Chemistry
1993 – 1997
Haiying Zhou's Publications
Dual functions of TAF7L in adipocyte differentiation
eLIFE
January 8, 2013
Authors: Haiying Zhou
The diverse tr... 阅读全帖 |
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i****l 发帖数: 579 | 18 发信人: slli (老良), 信区: SFworld
标 题: 终于完了
发信站: BBS 水木清华站 (Tue Dec 7 22:51:29 1999)
突然有些寂寞:以后聊天发呆的时候干什么呢?
----别和我说要我再打一本:)
作为基地系列,同时也是阿西莫夫整个银河系列的最后一部,这本
书指出了银河系的一种未来:Galaxia(银河体)。就是整个银河系,包括
人类、各种生物,还有无机物,直到每一个分子原子,都是一个共同意识----
Galaxia的一部分(看过〈基地的边缘〉这本书的应该对其中的一个共同意
识,Gaia有印象,在那里是整个星球作为一个共同意识存在。)。
如果有续集的话,我想至少有以下两个方面值得注意:
1。Galaxia会最终成立吗?事实上一直是机器人(Daneel为首)推
动着人类向着Galaxia成立。那么尽管Trevize作为能够由片面情况推出正确
结论的人,其认为Galaxia更好的想法是正确的吗?而Solaria人,作为另外
一种人,中性,有能量传导装置(Transduc |
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w**u 发帖数: 311 | 19 行禅在念住修行中有很重要的地位。跟大多数这儿的同学们一样,我没有福气有专门时
间在一个清静的地方做行禅。只能在上班路上做行禅,虽然效果不是很好,但千里之行
,始于脚下,希望为将来有机会密集禅修打下基础。
我曾经试着给每一步脚掌的移动分解标识“按,移,推,提”。开始感觉效果不错
,但缺点是标识经常跟不上所缘变化,导致标识变成了念口诀。后来请教卡玛兰蒂禅师
,她建议我只用察觉“左脚,右脚”。用这个方法我基本上能跟上所缘变化。但是很容
易走神被杂念打乱。到最后,我想了一个办法。这个办法跟我练习安般息念的经验有关
,我一般用数息(吸呼1-10)来使自己沉淀杂念。在行禅中,我也试着以“数步”让自
己能持续专注。我发现数七步是最好的:(一)容易持续专注;跟数息的道理一样。(
二)可进可退;如果环境吵杂,或专注力弱,可只觉知“一三五七”步。如果专注力强
,可以“一二三四五六七”左右脚全部觉知(三)易于平衡;因为是奇数,如果先标左
脚为一,那么下一循环右脚就标为一,就不会让觉知只偏向一侧。(四)从“七”到“
一”的变化点是打断妄念,觉知心念变化的最佳契点。
行禅是身念住的一种。身念住,用神经生物学的角... 阅读全帖 |
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r*******c 发帖数: 1060 | 20 她建议我只用察觉“左脚,右脚”。用这个方法我基本上能跟上所缘变化。但是很容
易走神被杂念打乱。到最后,我想了一个办法。这个办法跟我练习安般息念的经验有关
,我一般用数息(吸呼1-10)来使自己沉淀杂念。在行禅中,我也试着以“数步”让自
己能持续专注。我发现数七步是最好的
--------------我也无法坚持“左脚,右脚”,一会儿就走神了,等回过神来,发现已
经是““右脚,左脚”,囧。下次我也试着数七步,看是否适合我。谢谢啦!
身念住用神经生物学的角度来看,就
是对自己身体外周感觉系统和运动系统的变化进行持续觉知。
-----------------哦,这样子。说得好。赞!请问您能用类似这样子的表述解释一下
止禅吗?
我现在对大脑的attention system and rewarding system有蛮多的兴趣,另外,还对
“衰老”感兴趣,为什么会生“老”病死,成住“坏”空呢? (并因此对“熵/
entropy”这个概念产生了好奇心,不知道是不是可以对应佛教里的“劫”。哎,好好
奇。)
在组织学终末结构中,不同离子通道将不同的能量转化成细胞的电位变化,激发突触释
放神
经递质,... 阅读全帖 |
|
w**u 发帖数: 311 | 21 行禅在念住修行中有很重要的地位。跟大多数这儿的同学们一样,我没有福气有专门时
间在一个清静的地方做行禅。只能在上班路上做行禅,虽然效果不是很好,但千里之行
,始于脚下,希望为将来有机会密集禅修打下基础。
我曾经试着给每一步脚掌的移动分解标识“按,移,推,提”。开始感觉效果不错
,但缺点是标识经常跟不上所缘变化,导致标识变成了念口诀。后来请教卡玛兰蒂禅师
,她建议我只用察觉“左脚,右脚”。用这个方法我基本上能跟上所缘变化。但是很容
易走神被杂念打乱。到最后,我想了一个办法。这个办法跟我练习安般息念的经验有关
,我一般用数息(吸呼1-10)来使自己沉淀杂念。在行禅中,我也试着以“数步”让自
己能持续专注。我发现数七步是最好的:(一)容易持续专注;跟数息的道理一样。(
二)可进可退;如果环境嘈杂,或专注力弱,可只觉知“一三五七”步。如果专注力强
,可以“一二三四五六七”左右脚全部觉知(三)易于平衡;因为是奇数,如果先标左
脚为一,那么下一循环右脚就标为一,就不会让觉知只偏向一侧。(四)从“七”到“
一”的变化点是打断妄念,觉知心念变化的最佳契点。
我在上班常速走路练行禅时用这种7步法,每一步... 阅读全帖 |
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r*******c 发帖数: 1060 | 22 她建议我只用察觉“左脚,右脚”。用这个方法我基本上能跟上所缘变化。但是很容
易走神被杂念打乱。到最后,我想了一个办法。这个办法跟我练习安般息念的经验有关
,我一般用数息(吸呼1-10)来使自己沉淀杂念。在行禅中,我也试着以“数步”让自
己能持续专注。我发现数七步是最好的
--------------我也无法坚持“左脚,右脚”,一会儿就走神了,等回过神来,发现已
经是““右脚,左脚”,囧。下次我也试着数七步,看是否适合我。谢谢啦!
身念住用神经生物学的角度来看,就
是对自己身体外周感觉系统和运动系统的变化进行持续觉知。
-----------------哦,这样子。说得好。赞!请问您能用类似这样子的表述解释一下
止禅吗?
我现在对大脑的attention system and rewarding system有蛮多的兴趣,另外,还对
“衰老”感兴趣,为什么会生“老”病死,成住“坏”空呢? (并因此对“熵/
entropy”这个概念产生了好奇心,不知道是不是可以对应佛教里的“劫”。哎,好好
奇。)
在组织学终末结构中,不同离子通道将不同的能量转化成细胞的电位变化,激发突触释
放神
经递质,... 阅读全帖 |
|
w**u 发帖数: 311 | 23 【 以下文字转载自 ariya_sacca 俱乐部 】
发信人: wuyu (wuyu), 信区: ariya_sacca
标 题: 未完成的小愿
发信站: BBS 未名空间站 (Tue Jun 12 16:09:28 2012, 美东)
呈经雄心勃勃跟SeeU说要写一篇关与现代神经神经生物学对禅修机制研究的综述。但发
现现代的科学研究总体还是在验证阶段,再加上自己也没时间完备的学好佛法,就觉得
写机制综述还是太早了。特向SeeU道个歉。
我把绌文的全稿贴在这儿了。也不想去投稿了。等到自己有新体会,再修改。
其中有很多部分是来自于SeeU,JeanIris,和 CPath 法友的意见。在此谢过。
日常生活的止观修行--一个神经生物学者的叩道之路
关键词:行禅,安般息念,慈心禅,神经生物学.
文摘:两千五百年以前,佛陀的证悟为人类身心的完全自由指明了一条实修实证的道路
。其内容主要包括在“四圣谛,八正道,十二缘起的”的教诲中。佛法修行方法以戒清
静为基础,通过止观修行以增强“心”的定力和念力,最终培养其对世间万物本质的洞
察力,即“慧”力。时迁世移,... 阅读全帖 |
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d********m 发帖数: 102 | 24 http://sigmod07.riit.tsinghua.edu.cn/acceptedPaperForPODS.shtml
PODS 2007 conference has accepted 28 papers out of 187 submitted. They are
listed below in a random order.
Linfeng Zhang and Yong Guan.
Variance Estimation over Sliding Windows
Georg Gottlob, Reinhard Pichler and Fang Wei.
Monadic Datalog over Finite Structures with Bounded Treewidth
Joost Engelfriet, Hendrik Jan Hoogeboom and Bart Samwel.
XML Transformation by Tree-Walking Transducers with Invisible Pebbles
Bogdan Cautis, Serge Abi |
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H****S 发帖数: 1359 | 25 你太out了,打个:k 在Scala console下看看。scalaz是production ready library,
加上Scala特有的implicit type system,甚至比Haskell还要更加强大。Haskell现在
的潮流就是向scalaz export以争取更广泛的用户。Haskell machines / stream
transducer很牛吧,原作者自己export成scalaz stream,比rxjava理念先进不止一倍,
颦。 |
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h*i 发帖数: 3446 | 26 mainline进展就是transducer,可以比reducer更近一步提速。
但我觉得更主要的进展在于ClojureScript。
Facebook的react.js出来以后,让ClojureScript一下子变得非常有吸引力了,因为
ClojureScript的immutable data structure可以让react.js这种类似3D图形渲染的办
法变得更快。因为在ClojureScript里面,diffing几乎是免费的,只用检查reference
equality, 这就让基于react.js的ClojureScript库比native的react.js还要快,比如
om, reagent等等。
现在纯ClojureScript的库也出来了,甚至比reagent还要快,这样发展下去,用
ClojureScript搞Web UI可能会有大的突破。 |
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h*i 发帖数: 3446 | 27 没说mutable state不需要,这不是还用atom的么, 还是需要mutable state的。
component好像就是用atom实现的。
只是大家都没想到需要的地方这么少。Rich Hickey没想到,其他的Clojure early
adopters也没想到。但Clojure应用这么几年下来,就是发现了这么一个现象。
和atom比起来,ref, agent这些的确更难用一些,但几乎没有人用。而Clojure其他难
用的,甚至都很难搞懂的东东,比如core.async, reducers, transducers这些玩意儿
,一出来就有很多人用,说明难易不是主要问题。 |
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h*i 发帖数: 3446 | 28 听说过APL/J,以前IBM很多人都会。不过这种语言怪符太多了吧?point-free, 函数
的参数都是藏起来的,这个也太抽象了。
Clojure发展了好几年才发展到这个阶段,这就是Clojure 1.7引入的所谓transducer,
也就是0参数的函数组合。但这已经有人认为太过了,全是point-free的语言,还不得
让人发疯了。
我觉得语言设计掌握一个度很重要。计算机这个行当出新东西不容易,所谓The
sciences of the artificial, 都是人想出来的东西,大部分东东别人都已经想过了。
所以比的不该是谁“最“怎么怎么,比得是应该是谁把已有的东西组合得更好用。 |
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h*i 发帖数: 3446 | 29 没细看还支持?
Java好不好不是这个文章的问题,Java大多数人都很熟悉,有啥优点缺点大多数人都很
清楚,他讨论Java技术是不能起到哗众取宠的作用的。为了达到效果,他就没有根据的
对别的一堆语言胡喷瞎编一气,这就是这个文章的问题。
他喷的别的语言我不熟,Clojure我很熟悉。他小王对Clojure技术上一句实在的话也喷
不出来,只好说人Rich Rickey没CS学位,这也喷得太搞了,他小王不是一贯说啥学位
,名校,教授,文章等等都不重要么,他不是自己打自己的脸么?还有就是说Clojure
抄别的语言。说得好像计算机语言的所有特性都必须是原创才可以似的。那还能有任何
新语言么?语言设计的难点不正是如何选择组合已有语言特性么?
要说原创,Clojure倒还真有点原创的特性:
1. immutable data structure,这是受Phil Bagwell的几片数据结构学术文章的启发
得来的,但把这些作为一个语言的基础,Clojure是第一个。Clojure的实现和Bagwell
的文章描述也不太一样。
2. transducer,这完全是原创,其他语言都没有的。有很多人试图在... 阅读全帖 |
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n*****3 发帖数: 1584 | 30 transducer is cool. very
Immutable Data structure is good ,but other languages like scala , react.js
has that too..
Clojure |
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n*****3 发帖数: 1584 | 31 transducer is cool. very
Immutable Data structure is good ,but other languages like scala , react.js
has that too..
Clojure |
|
n*****3 发帖数: 1584 | 32 transducer is cool. very
Immutable Data structure is good ,but other languages like scala , react.js
has that too..
Clojure |
|
|
h*i 发帖数: 3446 | 34 immutable是会有存储和运行时间上的overhead,但这些都会被硬件进步和优化技术解
决。研究各种优化技术是计算机科学应该搞的正事,也是计算机科学进步的动力。
目前来说,对绝大多数应用,现有的immutable data structure的效率足够用了。以
Clojure为例,我还从来没听说过有人抱怨过Clojure运行慢的(抱怨启动速度慢的很多
,那是Clojure编译器在JVM上实现的问题,在Javascript上就没有这么明显的问题)。
存储空间的话,我的经验是Clojure程序和一般的Java程序内存消耗差不多。其实如果
仔细研究一下immutable的工作原理,就会发现, 其实它并不会占用更多内存,因为用
不到的数据都会被垃圾回收,而用到的数据immutable和mutable没有太多区别。当然
immutable对垃圾回收器的压力要大些。Clojure有transducer这类技术,可以减少计算
中间结果数据的产生。
除了函数可以被传来传去,还有就是函数都是带closure的,我觉得这很重要。 |
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M****e 发帖数: 70 | 35 talking about de novo peptide-derived drug design, i have an
idea using biosensor for such investigation. basically, i
would like to assess and evaluate the binding affinity of the
peptide to the known receptor as well as its downstream signaling
transductivity. so i may design two chips (biomembrane-based),
both approapriately covered by the receptors, with one linking
to some biosensor that basically detect the affinity, while the
other coupled to the primary downstream signal transducer while |
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d***e 发帖数: 243 | 36 我来补充一下,不是查google的,是三年前在北医帮导师写教材时我的一些理解.
Transfect一般是指各种artificial的手段给动物细胞内导入外源的基因片段.
Transduction ,conjugation涉及的是细菌间的基因转移,不关真核细胞的事.
1. 转化(Transformation).受体菌直接摄取供体菌游离DNA片段,转化的DNA片段可以是细
菌溶解 释放,也可以是人工提取. 摄取能力称为感受态 competence.应用吗,可以讲是最
早证实了DNA是遗传物质的Griffith的肺炎球菌实验.
2.转导 (Transduction). 温和噬菌体介导的遗传物质从供体菌到受体菌的转移.这里强调
的是temperate phage, 不是指溶原性噬菌体lysogenic phage.作用是细菌自发获得一些
新的protein,eg. toxin or antigen.
3.接合(Conjugation). 是plasmids介导的细胞间接触,是供体菌遗传物质进入受体菌.供
体菌遗传物质不一定就非是供体菌的gene,也可能只是供体菌的质粒transfer到rece |
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t*********r 发帖数: 31 | 37 For people to appreciate the work of Linda Buck, this is an overview by Shatz.
Linda Buck: 2004 Nobel Prize Co-Recipient
A Journey into the World of Sensory Perception
By Carla J. Shatz, PhD
Chair, Department of Neurobiology
Harvard Medical School
One of the most important areas of investigation in the neurosciences is the
mechanism of sensory perception. How do we transduce environmental stimuli
such as light, sound, and smells, into a neural signal, and then how is this
signal processed by the |
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a*********7 发帖数: 342 | 38 if you use lentivirus to transduce the cells and you see GFP expression,
your target gene should get integrated in the cellular genome. As others
said, maybe the promotor gets modified in the cell so that the target gene
can not be expressed well. |
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p****l 发帖数: 291 | 39 别叫俺前辈,haha
我没做过病毒载体感染DCs,但是做BMDCs非常多
实验室有人用病毒载体转染HSC,各个阶段前体细胞,还有pDC的
我能想到的建议就是
首先设置严格对照
1.bone marrow cells cultured with GMCSF for 4 to 5 days, facs CD11c vs.
MHCII
2.bone marrow cells transduced with empty retrovirus at day0, facs CD11c vs.
MHCII at day4 to 5, and plus GFP signal in facs
3.same as 2, but retrovirus carrying shRNA
4.before transduction with lentivirus, check CD11c vs. MHCII
5.letivirus also empty virus control and experimental vector
我觉得问题在病毒本身的可能性更大,我不知道她用什么载体
理论上,如果对照组CD11c表达... 阅读全帖 |
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s****9 发帖数: 932 | 40 I use retrovirus (MSCV) to transduce BM-Macrophage. Start to infect cells
1day after BM culture. Never have had any problem. Not sure about BM-DC,
but it is fairly unlikely that virus can influence DC differentiation.
I would suggest to try another viral vector or a commercial one.
vs. |
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l*****m 发帖数: 122 | 41 I've talked to quite a few people who used retrovirus to transduce BMM. And
none of them had experienced any problem. Not sure if it is because of the
vector since I've seen papers in which they used the same vector for BMDCs..
.but it is a good idea to look into other vectors...Thank you! |
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j******i 发帖数: 939 | 42 Gamma-retrovirus and lenti are both efficiently transduced into cells, but
gamma-retro can not infect non-dividing cells. Both Gamma-retro and lenti
insert into genome and thus have insertional mutagenesis risk, however,
gamma-retro usually inserts into transcription start sites and more likely
to disrupt genome gene expression. Lenti virus, however, is sort of safer
virus with no preference to start point while inserting.
Adenovirus is episomal virus and can only infect dividing cells. This mea... 阅读全帖 |
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j******i 发帖数: 939 | 43 You got me! Adv can transduce nondividing cells for sure! A PI who I
respected most told me that Adv can not
infect non-dividing cells, so I never doubt about it!
Yes, uncertain insertional property with preference to genes is a major
limitation of using lentivirus. Although
its insertion characteristic is modified by combining with transposon and ZF
, these chimeras are less efficient
in titer and transduction compared to wide type.
Totally agree that site-directed lenti will be a great achieve... 阅读全帖 |
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b**j 发帖数: 415 | 44
To my knowledge, no. for example, human primary cells normally can only be
passaged limited times, because it is telomerase-deficient; after transduced
with telomerase, it can proliferate indefinitely-immortalized, but still
not cancer cell. cells that proliferated indefinitely due to certain kind of
oncogenic mutations are cancel cells. I might be wrong.
don't know. |
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z*******6 发帖数: 679 | 45 Curr Mol Med. 2008 Aug;8(5):384-92.
Interleukin-4, interleukin-13, signal transducer and activator of
transcription factor 6, and allergic asthma.
Kuperman DA, Schleimer RP.
n******[email protected]
Thank you so much. |
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t********8 发帖数: 24 | 46 I have been in this field for more than 10 years. They are using a strategy
called "genetically modification of T cells with chimeric antigen receptors
(CAR)". The strategy has been around for >20 years. There are many on-going
clinical trials. Carl June is using "T body" to modified T cells for tumor
treatment. T body is basically a tumor-targeting single chain antibody (scFv
) with transmembrane and signaling domains for activation of T cells. When
transduced into T cells, it allows T cells to... 阅读全帖 |
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h******y 发帖数: 351 | 47 You are absolutely right about the ground-breaking contributions of Yamanka'
s to the iPSC. Thomson deserves credit for the first to isolate human ESC,
but his contributions in making human iPSC was not as great as Yamanaka.
Shinya is humble and kept telling the audience the story that it was his
postdoc's (Kazutoshi Takahashi) original idea to transduce all 20 factors
together to see if his hypothesis would work, which anyone would think to be
a "stupid idea". Alas, it worked and they were the... 阅读全帖 |
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m*****z 发帖数: 1451 | 48 depends on the stem cell you want to transduce
as
OK |
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A********2 发帖数: 107 | 49 If I understood your question correctly, you have inserted a Ab resistance
gene (linked to the mutation) into the mutant chr and were trying to move it
into wild type strain. Why not just make a phage lysate of your mutant
strain and then transduce the wild type strain. |
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j*******g 发帖数: 10 | 50 please email to j*****[email protected], thanks a lot!
Growth Factors. 2004 Sep;22(3):151-5.
Thrombopoietin signalling in physiology and disease.
Fishley B, Alexander WS.
Source
The Walter and Eliza Hall Institute for Medical Research, IG Royal Parade,
Parkville, VIC. 3050, Australia.
Abstract
Thrombopoietin (TPO) is a key physiological regulator of steady-state
megakaryocytopoiesis, the process of megakaryocyte production and maturation
that ultimately results in the formation of platelets. Ten years ha... 阅读全帖 |
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