t*****g
发帖数: 23 | 1 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
t*****g
发帖数: 23 | 2 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
f***i
发帖数: 31 | 3 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
z******o
发帖数: 52 | 4 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by August 10, 2012
$0 Balance Transfer Fee until 8/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
z******o
发帖数: 52 | 5 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by August 10, 2012
$0 Balance Transfer Fee until 8/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
f***i
发帖数: 31 | 6 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
s**u
发帖数: 9035 | 7 发信人: centrino1 (centrino), 信区: Immigration
标 题: J1申请NIW，自己的考古总结
发信站: BBS 未名空间站 (Sun Apr 8 22:14:18 2012, 美东)
我现在是J1签证，大陆PHD毕业，来美1年多，博后 DS2019 2012年10月到期。做了一些
办绿卡的功课，找了几个律师评估都说可以办NIW了，反正这个要等很久还是早办比较
好。EB1A 不够格，EB1B怕老板不会支持，而且打算近期换实验室。
因为我的case J1 涉及到WAIVE 换H1B 中间还要换实验室 ，NIW整个过程周期很长等等
问题，所以比较复杂，我考古了一周，消除了自己的一些疑问，同时也希望写下来帮助
大家。
首先我计划的步骤是：
1 申请140 等待140 approve
2 等待排期（几年） ，排期快到时先extension J1 再WAIVE J1，如果有条件就申请
H1B。
3 递交485
下面是一些问题的解答，如果有不正确的请指出：
1， 一定要H1B吗，涉及到是否需要自己老板支持自己的H1B.
一般交I140前最好续约J1，（不是... 阅读全帖 |
|
d*****u
发帖数: 20 | 8 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
k*******i
发帖数: 5 | 9 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
h********3
发帖数: 91 | 10 小弟现在OPT extension, 到年底结束。目前雇主帮我申请了一个20小时的PART-TIME
H1B，经过RFE和PP, 此PART-TIME H1B 刚刚通过,是10月1号生效。
8月底收到另一家公司的OFFER, 并且同意做H1B transfer.但公司一定要用自己的律师
，一个委内瑞拉人，通过几次电话，真心觉得水平非常一般，非常不放心让她HANDLE我
的CASE。非常想请有经验的中国移民律师，但公司不同意，说是怕RELEASE
information.郁闷死了。现在向大家确认几个问题：
1. 目前的H1B 刚通过，还没生效，要到10/01才生效。现在是否可以立即做TRANSFER?
2. 以现在雇主申请的H1B 的TITLE 与新公司的TITLE 并不特别MATCH.不知道这个在做
H1B transfer 的时候，会不会是个问题。其实新公司的工作和我的背景更MATCH。
3. 我也听说H1B transfer 其实就是重新申请一个H1B。唯一的不同就是不占名额了。
和老公司做什么，并没有太大的关系。是不是？还是说需要律师的好好包装，尤其是不
很MATCH 的前提下？... 阅读全帖 |
|
c*w
发帖数: 24 | 11 【 以下文字转载自 Visa 讨论区 】
发信人: chw (none), 信区: Visa
标 题: 急问： H4中信代签持H1B旧雇主的I797（配偶H1B 已经transfer到新雇主）
发信站: BBS 未名空间站 (Fri Feb 22 07:32:36 2013, 美东)
我大半年前H1B transfer从A公司到B公司，B公司的H1B已经approved，但老婆的H4没有
跟着一起transfer，上面显示的还是A公司的信息。现在她回国了需要代签H4签证（之
前一次H4签证成功过，但已经过期），中信银行的人说她需要新的显示有B公司信息的
H4 I797才能代签。我之前网上考古过都说H1B transfer，H4不一定要跟着transfer,只
要I797没有expire就可以，问了公司的律师也是这么说的，因为新公司B只给我办
transfer,不给H4一起办。
请问班上有类似经历的么，用旧公司时间办的H4 I797可以在配偶H1B transfer之后申
请签证么？还是中信银行的人不太懂这个细节？多谢！ |
|
g**u
发帖数: 583 | 12 感谢Yoyo版主的辛勤工作
关于transfer（relink), 希望确认一下步骤
（1）关于file的方法。
从置顶的link看到的是：http://www.mitbbs.com/article_t2/EB23/32138945.html
回答： The best practice is to submit an I-485 transfer request in an SRMT
through USCIS National Customer Service Center (NCSC) at 1-800-375-5283, or
online from USCIS website. SRMT is fastest method. An I-485 transfer
request may also be submitted in writing directly to NSC. 请保留SRMT case#，
或者
copy of written transfer request， 或者RFE response
现在的建议也有：一定要书面的，否则可能会受到rfe
所以到底是怎样... 阅读全帖 |
|
W********n
发帖数: 199 | 13 贡献一个，刚收到REF 体检通知
Time line:
PD: Nov. 1, 2013
Feb. 14, 2014: EB 2 140 approved
Aug 1, 2015: 485 submitted
Jan.-Feb. 2017: 准备EB3 140降级材料
March 1, 2017: 律师使用FedEx overnight 将140降级材料连同加急处理支票寄到
Texas center，确保USCIS在我的优先日current后收到材料
March 3, 2017: Texas center收到材料
March 7, 2017: 律师收到Receipt notice
March 13, 2017: EB 3 i-140 批准(USCIS 发给我律师电子邮件，降级成功，过后我才
从USCIS case status 那里刷出来状态)，一星期加急降级申请EB 3 140成功
March 19, 2017: 自己向USCIS要求把我家属的485从EB2 transfer到EB3，根据前辈的
经验，两条腿走路双保险：一是提交了Online SRMT，要求transfer 家属的E... 阅读全帖 |
|
r****z
发帖数: 30 | 14 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
j********i
发帖数: 27 | 15 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
t*****g
发帖数: 23 | 16 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
f***i
发帖数: 31 | 17 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
d*******u
发帖数: 10 | 18 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
z******o
发帖数: 52 | 19 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by August 10, 2012
$0 Balance Transfer Fee until 8/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
d*****u
发帖数: 20 | 20 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
j********i
发帖数: 27 | 21 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
f***i
发帖数: 31 | 22 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
t*****g
发帖数: 23 | 23 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
r****z
发帖数: 30 | 24 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
t*****g
发帖数: 23 | 25 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
r****z
发帖数: 30 | 26 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
f***i
发帖数: 31 | 27 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
d*******u
发帖数: 10 | 28 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
d*****u
发帖数: 20 | 29 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
f***i
发帖数: 31 | 30 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
z*****i
发帖数: 4 | 31 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
t*****g
发帖数: 23 | 32 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
r****z
发帖数: 30 | 33 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
z******o
发帖数: 52 | 34 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by August 10, 2012
$0 Balance Transfer Fee until 8/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
z******o
发帖数: 52 | 35 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by August 10, 2012
$0 Balance Transfer Fee until 8/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
j********i
发帖数: 27 | 36 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
t*****g
发帖数: 23 | 37 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
z******o
发帖数: 52 | 38 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by August 10, 2012
$0 Balance Transfer Fee until 8/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
j********i
发帖数: 27 | 39 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
z******o
发帖数: 52 | 40 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
r****z
发帖数: 30 | 41 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
d*****u
发帖数: 20 | 42 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
r****z
发帖数: 30 | 43 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
j********i
发帖数: 27 | 44 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
z******o
发帖数: 52 | 45 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by August 10, 2012
$0 Balance Transfer Fee until 8/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
f***i
发帖数: 31 | 46 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
r****z
发帖数: 30 | 47 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by July 10, 2012
$0 Balance Transfer Fee until 7/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
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n********e
发帖数: 1630 | 48 大家好，
有个western transfer的technical的问题。我们lab用的是一个很笨重的biorad的semi
-dry transfer 仪器。我刚开始做western，transfer的效率总是很差。
protein ladder用的是5ul，
manual上推荐mini gel，8.5cm x 5.5 cm，用10v-15v，30min
我开始用过25v，总是看到有的ladder 到了下面的filter paper上面，同时还有ladder
在gel上面，如果时间长点，gel上就看不到ladder。
后来我用15v，推荐的，40min-60min，还是看到有ladder在gel上面，但是没有在
filter paper上面。说明这个voltage很合适的，不会over transfer。但是高分子量的
ladder， 50kd以上的，总是转不完全。
我的protein只有20kd，理论上根据ladder来看，应该都转过去了。可是我的western跟
同样的sample，在其他lab做的，结果就不一样。抗体都是按照他们的catalog number
买的。但是dete... 阅读全帖 |
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z******o
发帖数: 52 | 49 Discover More Card released a new offer: No Balance Transfer Fee
http://goo.gl/6gb2D
• Limited Time Offer - $0 Balance Transfer Fee!*
0% intro APR on purchases for 12 months.
0% intro APR on balance transfers for 12 months from date of first transfer,
for transfers under this offer that post to your account by August 10, 2012
$0 Balance Transfer Fee until 8/10/2012. After that, either $10 or 5% of the
amount of each transfer, whichever is greater. |
|
