J******s
发帖数: 7538 | 1 第三节 我和无我：Self and no-self（a paradox）.
“The true value of human being is determined primarily by the measure and
sense in which he has attained to liberation from the self.”
----Einstein
“The longer our lives, the more we will have a chance to see that there is
no self living in them.” ----James Hughs
(1) 非常有意思，如果你去查一本小说里出现频率最高的词是什么，答案往往是“我”。
西方心理学把“我”看作是mind的一种功能，通过这种认知我们在意识层面来组织并解
释自己的经历。“我”更多时候是一种transi... 阅读全帖 |
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T*******y
发帖数: 6523 | 2 Yes, thank you, I understand this, and I want to know why the fact that
things refresh at the smallest scale possible has effects on our usual
understanding of the world.
The frames are here, this notion sits well with me, and I fully accept that
everything is transient and ever changing.
My question is why the frames are so much in order, but not just random?
I guess that I want to say that the order of the frames is also very
meaningful, but the fact alone that there are refreshing frames does... 阅读全帖 |
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T*******y
发帖数: 6523 | 3 I think I know the difference in our opinions now. It really looks like two
sides of a story.
I agree that the feelings are transient and either they are here, or they
are gone. Mindfulness surely happens here and now.
However, the feelings are easily drawn out by certain thoughts. For example,
when one replays a certain scene in mind, that scene does not happen here
right now, but just a thought about it makes one feel in the same old way,
and similarly, another turn-around thought about it mak... 阅读全帖 |
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Y**u
发帖数: 5466 | 5 ☆─────────────────────────────────────☆
TrueStory (不是幸福的坑不挖) 于 (Fri Nov 11 13:43:09 2011, 美东) 提到:
http://evelynrodriguez.typepad.com/pointingtothemoon/2007/02/by
Above is a description on how she got her knowledge, and she experienced that no-me and no-name feeling.
I like that whole blog, and I will zz the full blog below.
The four questions are actually only one question "is it true?", as no thought is true. But for people not yet there, such as me, choosing the truer thought is a re-entr... 阅读全帖 |
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b**d
发帖数: 7644 | 6 没有你的世界，我的世界这个问题。 See where your problem is ? 你问的question,
从开始就是不存在的，是你自己制造的。 镜花水月，你不去看真实的世界，却不停的
研究幻象。
那，I can tell you. before you realize the thoughts and feelings are
transient, they comes and goes, and learn how to not stick to it. You will
always suffer. Like you are suffering now. |
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b**d
发帖数: 7644 | 7 你也是的。善不善都是transient的事情，跟人没有直接联系，跟具体的事情有关。 牵
涉具体利益，很少人会善。就这么个小版，就为灌个水，谁会没事干玩那个心计呀？
除了那种想自己当大师，诈骗的人。 我怎么瞅，怎么不觉得Yisu想自己开山当祖师。
。。。。。。
这不明白着的吗？ 这个就是灌水，聊天，吵架，瞎玩的地方。Clear? 你这人就是爱较
真。。。。
俺觉得只要不在班上搞捐款，不卖东西的都是好人。 |
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Y**u
发帖数: 5466 | 8 ☆─────────────────────────────────────☆
waterer (快义恩仇之灌水山寨王) 于 (Fri Dec 23 21:08:17 2011, 美东) 提到:
我靠，老子就走了那么几天，没法上网，
你们这帮猴子就闹成这样。。。
☆─────────────────────────────────────☆
robbin (robbin) 于 (Fri Dec 23 21:12:54 2011, 美东) 提到:
☆─────────────────────────────────────☆
waterer (快义恩仇之灌水山寨王) 于 (Fri Dec 23 21:36:34 2011, 美东) 提到:
你给个摘要吧，老子实在没心思去翻那十几页
的吵架贴。俺这才走了几天啊，就这样了。
☆─────────────────────────────────────☆
robbin (robbin) 于 (Fri Dec 23 21:48:16 2011, 美东) 提到:
☆──────────────────────... 阅读全帖 |
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c*******n
发帖数: 1648 | 9 现在我有个无限长TUBE，外面恒温盐浴T0加热，里面绝热空气。问里面什么时候能到一
定温度T。这是TRANSIENT HEAT CONDUCTION PROBLEM。目前只找到SLAB的解析解，
哪位能告诉圆筒坐标TUBE的解析解在哪找？有大尺寸的CURVE图，查表也都可以。最好
网上有，能让化工原理学过半册的看懂。。。公司里去图书馆不方便。谢谢！ |
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f*****r
发帖数: 21 | 11
感谢大家的踊跃发言!
So let me make a summary:
享乐主义 can be put into English in the following way:
hedonism or Epicureanism.
And it would be rather easy for general people to understand
if you use: transient sensualism. I seem to prefer this guy :)
BTW. 刚才有位同学提到 gastronomy , 我象他多半是用金山词霸查的.
金山词霸可以用来快速检索,但是如要准确掌握词义,最好使用权威的
英文字典. |
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j****e
发帖数: 1192 | 12 POSTDOCTORAL POSITION IN LASER SPECTROSCOPY AND PHYSICAL CHEMISTRY
>
>A postdoctoral position is available in the laboratory of Dong-Sheng Yang
>at the University of Kentucky. The project involves the synthesis,
>spectroscopy, and structures of transient metal complexes and clusters in
>the gas phase. The metal-containing species are produced by laser
>vaporization, identified by photoionization time-of-flight mass
>spectrometry, and studied by pulsed-field ionization ZEKE photoelectron,
>mass |
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w****p
发帖数: 6 | 13 比较两个教授的publication，大家觉得哪个好？
#######################################
教授A
Selected Publications:
1. Xin M. Liang, Weiping Ding, et al., Microfabricated thermal conductivity
sensor: a high resolution tool for quantitative thermal property measurement
of biomaterials and solutions, Biomed Microdevices, (2011)13: 923–928. (
SCI&EI, IF:3.386, JCR:2区)
2. XM Zhou, ZQ Shu, WP Ding, et al., Heat transfer analysis for the design
and application of the passive cooling rate controlled device-Box-in-box,
Int ... 阅读全帖 |
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J******s
发帖数: 7538 | 14 【 以下文字转载自 Wisdom 讨论区 】
发信人: JeanIris (Iris), 信区: Wisdom
标 题: 【五月的鲜花】「我」「无我」和「正念」（3）
发信站: BBS 未名空间站 (Tue May 31 13:00:00 2011, 美东)
第三节 我和无我：Self and no-self（a paradox）.
“The true value of human being is determined primarily by the measure and
sense in which he has attained to liberation from the self.”
----Einstein
“The longer our lives, the more we will have a chance to see that there is
no self living in them.” ... 阅读全帖 |
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l******k
发帖数: 27533 | 15 【 以下文字转载自 Pharmacy 讨论区 】
发信人: lilybank (炎炎夏日何处去，采得青莲一茎香), 信区: Pharmacy
标 题: Re: 急问，妈妈睡眠不好，请问有什么好的保健药么？
发信站: BBS 未名空间站 (Tue Mar 13 21:51:34 2012, 美东)
nice search from Beer's list.
I don't look at it, but I believe the first generation antihistamine is not
contraindicated in the elderly. Reference is black and white, but the
patient is specific, the appropriate recommendation should be made based on
the patient's situation. Antihistamine is not the best drug for the elderly
insomnia, but for th... 阅读全帖 |
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c***k
发帖数: 1589 | 16 wifi is meant to be shut down to save the power.
so it is perfectly normal to see 3g transiently
siri |
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w*r
发帖数: 2421 | 17 remote table 加一个column -- last update time stamp, 然后local写一个程序吧
remote的data在凌晨给拖过来不就完了？
不过问题为什么你的application不能访问remote的database呢？总是一个麻烦的事情
。当然oracle/db2等平台都有duplication service. 还有第三方的vendor提供比如说
golden-gate之类的，主要的思想是一台数据库上有insert/update/delete的时候吧
undo/redo log或者transient journal给同步到另外一台机器上去。
local |
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m******t
发帖数: 2416 | 18
Did you try just JMS without MDB, also making the queue transient? |
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r*****l
发帖数: 2859 | 19
没有那样复杂，只是简单的数组。里面的元素是链表。但是这个数组是transient。所以才有第二个问题。 |
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r*****s
发帖数: 985 | 20 i think this is what goodbug means:
@Lob
@Column(name = "SOMETHING")
private byte[] serializedSomething;
@Transient
private Something something;
public setSomething(Something details) {
serializedSomething = serializeSomething(details);
}
public Something getSomething() {
if (something != null) {
something = deserializeSomething(serializedSomething);
}
return something;
} |
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g*****g
发帖数: 34805 | 21 Transient data, data that can be rebuilt, data that can afford
a little inconsistency, cache replacement etc.
Using it for financial transaction is calling trouble.
A hybrid SQL/NoSQL application is very common. You have to be
very careful on what you are doing.
If you have an existing application, be conservative and try
table by table. |
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z*******3
发帖数: 13709 | 22 之前说过我对abstract class的看法，倒是引来不少非议
尤其是有些人居然举出了例子，好，我们就从这个例子开始
有人说在这种情况下要使用abstract class
比如一个animal，有walk和sing方法
那么代码就是
public abstract class Animal{
public void walk(){System.out.println("walk")};
public abstract void sing();
}
然后对于具体的实现类，比如Cat，有如下实现
public class Cat extends Animal{
public void sing(){
System.out.println("cat sing");
}
}
这样一个类，好处就是“便于扩展”等等
OK，那么我们就从这个例子出发，说说为什么在j2ee环境中，我们不这么做
然后说说会怎么做
首先，在j2ee的环境中，关于animal这种实体
我们会在各个层面建立entity
比如在db层面建立一个表，叫做animal，然后有一个cat记录
然后通过orm，建立起一个dto之类的玩... 阅读全帖 |
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z*******3
发帖数: 13709 | 23 之前说过我对abstract class的看法，倒是引来不少非议
尤其是有些人居然举出了例子，好，我们就从这个例子开始
有人说在这种情况下要使用abstract class
比如一个animal，有walk和sing方法
那么代码就是
public abstract class Animal{
public void walk(){System.out.println("walk")};
public abstract void sing();
}
然后对于具体的实现类，比如Cat，有如下实现
public class Cat extends Animal{
public void sing(){
System.out.println("cat sing");
}
}
这样一个类，好处就是“便于扩展”等等
OK，那么我们就从这个例子出发，说说为什么在j2ee环境中，我们不这么做
然后说说会怎么做
首先，在j2ee的环境中，关于animal这种实体
我们会在各个层面建立entity
比如在db层面建立一个表，叫做animal，然后有一个cat记录
然后通过orm，建立起一个dto之类的玩... 阅读全帖 |
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a******s
发帖数: 7 | 24 Test Question [1]
Given:
5. class NoGo implements Runnable {
6. private static int i;
7. public synchronized void run() {
8. if (i%10 != 0) { i++; }
9. for(int x=0; x<10; x++, i++)
10. { if (x == 4) Thread.yield(); }
11. }
12. public static void main(String [] args) {
13. NoGo n = new NoGo();
14. for(int x=0; x<101; x++) {
15. new Thread(n).start();
16. System.out.print(i + " ");
17. } } }
Which is true?
A. The output can never contain the value 1... 阅读全帖 |
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z****e
发帖数: 54598 | 25 E
CD
A-是否Serializable，只需要本类继承接口就行了，跟父类无关
B-理由同上，这个是markup interface，看到接口，就可以序列化
E-volatile和transient故意混淆 |
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b***y
发帖数: 2799 | 26 ☆─────────────────────────────────────☆
ilvch (From here to eternity) 于 (Sat Jul 12 23:45:03 2008) 提到:
一个code snippet:
while (a!=1){
if even(a) a=a/2;
else a=3*a+1;
}
问这个程序会不会在某种输入(a>0)时陷入死循环？
☆─────────────────────────────────────☆
repast (xebec) 于 (Sun Jul 13 00:11:47 2008) 提到:
这个是一 countable markov chain, but definitive,
u need to judge if it is recurrent/transient for every initial state.
☆─────────────────────────────────────☆
goodbug (好虫) 于 (Sun Jul 13 00:14:22 2008) 提到:
我猜 |
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r**u
发帖数: 1567 | 27 http://www.agner.org/random/
We used this library to simulate the appearance of transient fault on the
processor, which follows poisson distribution. |
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c*********e
发帖数: 16335 | 28 en，cookie有很多种。我说的session cookie只是存在内存里，不是一个存在hard
drive的physical file.这个区别很大。一个在内存，一个是文件。
A session cookie, also known as an in-memory cookie or transient cookie,
exists only in temporary memory while the user navigates the website. Web
browsers normally delete session cookies when the user closes the browser. |
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s*****t
发帖数: 1994 | 30 日出”望远镜上的IMaX仪器不仅描绘了太阳表面特征，而且还使太阳磁场变成有形之物
，易于人们看到。太阳磁场在偏振光里呈现出黑色或白色结构。
“日出”望远镜拍摄的图片，显示了近紫外线中4种不同波长范围里所谓的颗粒状结构
。这种光结构是太阳磁场的基本元素。
新浪科技讯 北京时间11月18日消息据美国宇航局太空网报道，用气球搭载的“日出(
Sunrise)”望远镜拍到了有史以来最为详细的太阳表面翻涌沸腾的景象，图片展示了太
阳表面看起来像颗粒状的东西和移动的气泡等瞬变暗斑(transient dark spot)，凸显
了太阳表面的工作过程。
6月8日，“日出”望远镜从瑞典北部基律纳雅斯兰吉(Esrange)航天中心发射升空
，它是迄今为止离开地球的最大的太阳望远镜。这个6吨重的望远镜悬吊在一个直径是
427英尺(130米)的巨大氦气球上。发射升空以后，“日出”升到地球上空37公里处的高
度。处于这个高度，该望远镜所在的地球大气层(同温层)的观测条件，跟从太空进行观
测的条件基本类似。空气湍流对“日出”产生的影响，并不像对地球上的望远镜产生的
影响，“日出”望远镜可以观测到太阳发出的紫外线， |
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R*s
发帖数: 2041 | 31 for stable transfection: u include a selective marker (such as G418,
puromycin) in the DNA construct will transfect cells with. Then by
including the antibiotics (G18, puro...) in the media, you will be
able to seletively grow up the cells trasfected with your construct, while
killing the untrasfected ones.
For transient transfection: you don't have such selective markers in
the constracuts. You will always grow cells in regular media. Since it
is impossible to achieve 100% transfection, there i |
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r**r
发帖数: 171 | 32 A good CSF extract should be arrested @ metaphase.
After the addition of calcium or fertilization which causes a transient
increase in cytoplasmic calcium conc., the CSF will be inactivated, following
by APC activation, cyclin B destruction and mitotic exit. And this process is
roughly 30 min. It is easy to collect phosphorylated separase before the
addition of calcium. To collect the unphosphorylated one, like you say, you
can throw sperm chromatin into extract and monitor the progresstion by D |
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m**o
发帖数: 13 | 33 The time for assay your transient expression depends on your host cell, transcrioption
effeciency,posttranslation mechanism etc. In one words, there are many may have
effect on your expression assay. So people used to do a progressive assay to
determine the best time point for your assay.
According to different transfection protocol, there is difference of incubation
time before conducting the assay.
The mechanism of transfection between Lipo and Calcium is diferent. For the
different cases, the |
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e****a
发帖数: 17 | 34 Maybe you can try different protocals by using only EGFP, and check the cells
at differnt time points ,you will be able to see the EGFP expression by
noticing the green cells under fluorenscent microscope, so that you are able
to tell the time course of plasmid expression in different protocals.
And different plasmids have differnt expression patterns, I would try to
harvest transfected cells at different time points and measure the protein
level.
usually people harvest transient transfected cel |
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t****p
发帖数: 1504 | 35 由于kinase和substrate的binding是transient and weak 的，所以这一直是个难题。
1. 直接binding，yeast-two hybrid，或通过complex中找到。这些报道很少，一般
是找到后用来确证的确直接binding。
2. 根据已知的substrate中磷酸化位点序列的保守性来scan，推测，猜。
3. 猜：两个蛋白功能很接近，看是否直接binding，是否磷酸化。
4. 用peptide array：体外纯化的kinase，看对哪些peptide的磷酸化活性高，根据
peptide的序列scan，再体内验证。
5. 2-D胶。敲除某个kinase，看哪些蛋白的磷酸化发生变化。再质谱鉴定。
6. inhibitor array：现在有很多专门针对某个kinase的小分子inhibitor，把这些
inhibitor收齐全了，如果有某个substrate的磷酸化抗体，就用inhibitor筛一遍，看
哪个抑制强。
7. ……看我的第二篇文章：鼓吹phosphoproteomics的文章……
欢迎补 |
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O******e
发帖数: 4845 | 36 ☆─────────────────────────────────────☆
lanceandfei (La Jolla的云彩) 于 (Wed May 23 18:58:37 2007) 提到:
Journal Club 挽救老年痴呆症的希望 1
Recovery of learning and memory is associated with chromatin remodelling.
Nature. 2007 May 10;447(7141):178-82.
前期工作： Opposing roles of transient and prolonged expression of p25 in
synaptic plasticity and
hippocampus-dependent memory.
Neuron. 2005 Dec 8;48(5):825-38.
Aberrant Cdk5 activation by p25 triggers pathological events leading to
neurodegeneration and
neurofib |
|
t****p
发帖数: 1504 | 37 最近想在一个比较原始的细胞上建个大的突变库，发现在这个物种上转质粒随机
integrate到genome上的效率大概为1/2000。按这个效率，我需要转上千次才能达到我
要求的库容量。
我怎么记得人的细胞转病毒的话转化率可以高到90%以上？如果是质粒transient
tranfection的话，效率有多高呢？我在想这个原始细胞又没有细胞壁，大小也跟人的
培养细胞差不多，转化效率的上限是否能跟人的细胞差不多。哪怕是转化效率提高几倍
，也能大大减少我的工作量。
OK，问题是：
１：人的培养细胞的转化效率有多高？
２：一般情况下，提高转化效率可以从改变哪几个方面的参数入手？（我用电转，不排
斥其他方法）
３：有一点不太明白，比如人的细胞共同转几个质粒（比如产生ｉｐｓ的时候就共同转
了２４个病毒质粒），为什么转化效率不会大大下降？有人提出说质粒可以缠绕在一起
，一起转化，我很难接受这样的理论。
哪位达人解惑一下。 |
|
n********k
发帖数: 2818 | 38 Could you please do a transient transfection for me to the target cellls for
me and also do western to check
whether the protein is expressed in 293 cells(just collect the cells after
your collect the supertant for virus? I
suspect two things: either promoter silencing in ur target cells or ur
construct is bad...or it could be ur protein
is very tightly regulated posttranscriptionally, anyway, there could be many
possibility for this to happen... |
|
s********s
发帖数: 84 | 39 谢谢。。western blot，免疫荧光都作过了。。
只要是transient transfection 不管是WB还是免疫荧光都可以出来。。
不过我倒是真的没有检测过用来packing的293里面有没有蛋白。。我可以试试，但是我
觉得应该是有的
就是从用293细胞produce virus开始，往后就不行了。。
如果是promoter silencing的话 是不是就没别的办法了。。
不知道为啥以前是怎么做出来一次的。。要是从来没做出来过，也就死心了 没啥好说的
曾经做出来过可以用的virus，出了一些结果，然后把那批virus用完了。。之后就再也
做不出来能用的了。。plasmid都是同一管啊。。
好吧。。其实我也知道这问题只有上帝才能回答。。要那么容易回答，我老板早就回答
我了，他也说有些问题就算是作virus很多年的人也不一定能搞明白啥问题，但是问题
在于老天，能不能让我突然很好运的把这个virus毫无理由的再做出来一次么。。
不管能不能解决问题，我谢谢回答了我问题的人。。至少我抱怨了。。= =
for
many |
|
n********k
发帖数: 2818 | 40 BTW, the way u are doing is likely fine...within several passage is fine or
as long as they are well-chaterized, it is fine...but is it that hard to go
retroviral and treat them as transient approach...I rarely keep any stable
cell lines now...And for many studies, it is impossible or not right to keep
stable cell lines because the manipulation cause them to change...for
example differentiation...so u are screening against the correct phenotypes.
..anyway, nothing is absolute and it all depends. |
|
p**i
发帖数: 3525 | 41 xiexie，很有道理。那如何做才是现代的最有说服力的细胞实验呢? 我们一般是加例如
GFP-tag，
然后用retroviruses转染建立几个细胞系，然后看接受信号后的蛋白移动。
inducible clones 是否比较不过时有说服力?
transient
many
one |
|
h**********8
发帖数: 650 | 42 Biomicro is right.
The purpose of Lentivirus is to get stable transfected cell line
Ordinary plasmid can get hight expression but is transient.
CMV promoter is virus promoter, cells can silcent it. I get this information
from my boss, but I have not found this paper. |
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s******y
发帖数: 28562 | 43
有蛋白产生？ 非常疑惑 。
Most plasmid for transient expression will carry their own promoter upstream
of the expression codon.
DNA可以吗？
single strand DNA is degraded easily and practically not ideal for
transfection.
本不整合到细菌基因组的吧。我们摇菌用的细菌自己有自己的质粒对不对？
Yes. Most of them have their own replication origin site.
If you look at the vector map you will notice things like "F1 Ori". |
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s******y
发帖数: 28562 | 44 You need to look at the sequence elements of the double strand DNA you are
using.
Most of them should carry a promoter. If not, then they won't get expression
in transient transfection. However, if they got integrated into chromosome,
they will.
I don't know how you get these questions, because they are not "real
questions".
It all depends on how you design the DNA for transfection therefore there is
NO real answers to it. |
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q*****n
发帖数: 331 | 45 Plasmid DNA does not replicate in mammalian cells.
The only thing they do is to provide a template for RNA transcription
because they all contain strong promoter such as CMV.
Becaue they don't replicate, their level will decrease and dispear after a
few days, that's why they are called "transient expression".
At low frequency, they may intergrate into genome, then they can replicate
along with the genome. You can use antibobitic to select this low frequent
clones, that is called "stable clone se |
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a****l
发帖数: 125 | 46 诸位大侠可知到用什麽方发transfect siRNA 到 MEFs cells 里面吗? 要 transient
的transfection 就可以. 貌似MEFs 不太好转染的说. Lipofectamin 2000 可以吗?
谢先 |
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a****l
发帖数: 125 | 47 我要knockdown 的蛋白对cell proliferation 很重要。害怕用lenti-shRNA 后细胞不
长了。所以想用transient 的siRNA. 但具体Lenti-shRNA 会不会造成这种后果也不清
楚。还请在坐赐教。 |
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D*a
发帖数: 6830 | 48 Cerebrovasc Dis. 2009;27(5):509-18. Epub 2009 Apr 16.
Rationale and design of a randomized, double-blind, parallel-group study of
terutroban 30 mg/day versus aspirin 100 mg/day in stroke patients: the
prevention of cerebrovascular and cardiovascular events of ischemic origin
with terutroban in patients with a history of ischemic stroke or transient
ischemic attack (PERFORM) study.
Bousser MG, Amarenco P, Chamorro A, Fisher M, Ford I, Fox K, Hennerici MG,
Mattle HP, Rothwell PM; PERFORM Study Inv |
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h****n
发帖数: 2552 | 49 Transient transfection后你的蛋白主要在胞质还是在核里面？有些蛋白over express
后会change localization |
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t****p
发帖数: 1504 | 50 说到肿瘤的heterogeneicity，我来说点外行的想法。
就是说stem cell的概念不是绝对的，孤立的。所以人们引入了全能、多能的概念，又
引入progenitor,transient amplification cell的概念，目的是把连续的事件人为划
分为可描述的几个阶段。
相似的，在我看来找绝对概念的CSC是有问题的。一个肿瘤里面不应该只有两个细胞群
体，能重新形成肿瘤的csc和不能形成肿瘤的一般细胞，而是说每个细胞能形成肿瘤的
能力有高有低而已。所以，人们设想可以通过消灭一个肿瘤细胞中的特定细胞群来消灭
肿瘤的想法是误入歧途的。 |
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