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全部话题 - 话题: variant
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G***G
发帖数: 16778
1
来自主题: Biology版 - variants
问个用genetic variant or mutants for biomarker的问题
假设,你发现了一个variant,它的高表达表示癌症,低表达表示正常
但是如果没有发现这个variant,则无法预测。
那么,你怎么能把这个biomarker用在blind testing set中呢?
因为testing set是blind,可能出现这个marker根本就不存在这个病人中
也就是无法预测。但是有时候,不存在和低表达之间很难区分,就是0和0.0005的关系。
而且不同的样本这个threshold很难一样。有的样本0.5才是低表达,有的样本0.00005
才是低表达。
请问这个问题如何解决?
b***k
发帖数: 2673
2
☆─────────────────────────────────────☆
iambear (iambear) 于 (Wed Sep 17 22:40:36 2008) 提到:
我在vb的marco里有个variant,要用这个variant,但vb function里的Input是range,一
样的数据,怎么把variant 转成range啊? 一切都不涉及excel spreadsheet. vb搞真麻
烦啊,一行能解决的东西要写好几行
☆─────────────────────────────────────☆
iambear (iambear) 于 (Thu Sep 18 02:54:29 2008) 提到:
BTW,vb想要表示所有行第一列怎么表示?
☆─────────────────────────────────────☆
alexx (panda in love~八胖~饲羊员~水木十年) 于 (Thu Sep 18 02:56:10 2008) 提到:
那不就是第一列么

☆────────────────────────────
r*********r
发帖数: 3195
3
来自主题: Programming版 - boost::variant 的问题
有什么好的文档解释variant 设计的吗? 直接看源码好累啊.
读了 andrei alexandrescu 的文章 "an implementation of discriminated unions
in c++"
还比较好懂. 但是 boost 的这个 variant 好像要复杂很多.
btw, 是一定要看懂 mpl 吗?
S******e
发帖数: 393
4
有一个目的我知道就是产生不同size的isoform,这个好理解。
但我在NCBI里查一个gene, 它有几个不同的transcript variant,但貌似只是
UTR长短不同,产生的蛋白是一样的(即同一个isoform),那要这种不同的transcript
variant什么用, 控制translation level,调节表达量? 还有其他作用吗?
M*4
发帖数: 2937
5
来自主题: ebiz版 - Galaxy S II 的variants (转载)
【 以下文字转载自 PDA 讨论区 】
发信人: MI4 (暂缺), 信区: PDA
标 题: Galaxy S II 的variants
发信站: BBS 未名空间站 (Tue Jan 31 13:39:07 2012, 美东)
T989, i777, i727, international...到底哪款更好一些?
j*******6
发帖数: 429
6
刚收到一封信,有关 my baby's newborn screening blood test results.
上面说:
Your baby has a variant hemoglobin trait.
this means that each red blood cell contains both normal hemoglobin and an
unusual hemoglobin.
大家有收到过这样的信么?是很严重的问题么?该怎么办?
r*********r
发帖数: 3195
7
来自主题: Programming版 - boost::variant 的问题
确实找不到文档. 而且 variant 和 mpl 的作者好像都是小本, 会写代码不会解释.
今天读了一篇 mpl 的文章, 看得火大.
R*s
发帖数: 2041
8

Normally it takes 3-5 hours after transfection to express enough GFP protein
in cells to get detectable fluorescence. Another trick is that it will take
about 3 hours for the fluorophore on GFP to be oxidized so that it can be
fluorescent. As far as I know, the quickest detection of GFP (or other
living fluorescent variants from Clontech) is around 8 hours after
introduction of DNA. The earliest time I ever tried is 10 hours after
transfection, but it is weak.
However, if you really want to see
h******y
发帖数: 1374
9
请教如何在PCR的引物的设计中能够规避splice variants的问题?
h**********r
发帖数: 671
10
Title:
Functional characterization of eight human cytochrome P450 1A2 gene variants
by recombinant protein expression
The Pharmacogenomics Journal 10, 478-488 (December 2010) | doi:10.1038/tpj.
2010.2
My email is m******[email protected]. Thank you so much!
y*******t
发帖数: 91
11
来自主题: Biology版 - How to find variants
想知道某段序列是否有不同的variants, 或者说有没有SNP存在?怎么查啊?
Thanks a lot
t*********1
发帖数: 418
12
来自主题: Biology版 - ChR2 variant 求助
有没有那位同学知道 "ChR2 variant with prolonged inactivation kinetics"?
Is ChR2(H134R)a good example?
Thanks!
Z******5
发帖数: 435
13
问题带有误导性,基因不是“主动”去产生多个transcript variants,应该是一些偶
然的因素导致基因扩增,没有导致什么严重的后果,甚至可能有优势,就保存下来了。
s**e
发帖数: 1523
14
老板让我找一个基因所有的RNA splicing variants...我一头雾水。请大家帮忙!!!
o*****a
发帖数: 2335
15
UCSC Genome Browser就行,主页上选gnomes,找你要的动物,搜索基因,在browser中
把UCSC Genes菜单选成full,缺省设置就会显示所有splicing variants
s*****3
发帖数: 20
16
My 2 cents:
Try RT-PCR, easy to do and easy to get answers. You can use the 7 predicted
variants in Ensembl as guidance to design your two primers. The handbook
in the link below is a good reference.
http://www.qiagen.com/Products/Catalog/Assay-Technologies/End-P
D***0
发帖数: 5214
a********2
发帖数: 2561
18
来自主题: _Auto_Fans版 - VW出了个柴油Golf Variant(wagon)
今天新闻说汽油版variant在日本上市,280马力(法规限制),起价550多万日元,还
不算optional呢。我擦,照这个价格,就算来了美国也根本卖不动嘛。
欧版的是300马力,售价未知。
s******9
发帖数: 283
19
NCBI protein database一般都有标注吧。
FEATURES Location/Qualifiers
source 1..503
/organism="Homo sapiens"
/db_xref="taxon:9606"
gene 1..503
/gene="TGFBR1"
/gene_synonym="ALK5"
/gene_synonym="SKR4"
Protein 1..503
/gene="TGFBR1"
/gene_synonym="ALK5"
/gene_synonym="SKR4"
... 阅读全帖
w*******y
发帖数: 60932
20
Tillys.com has a code for an extra 50% off sale: SALE50
This means some great deals on men's Levi's, including:
LEVI'S 514 Slim Straight Mens Jeans:
http://www.tillys.com/tillys/variants.aspx?prod=172300821&ctlg=010_Guys&cid=999&source=8&size=&s=
- $15
LEVI'S 511 Skinny Extra Slim Mens Cords in black:
http://www.tillys.com/tillys/variants.aspx?prod=170231100&ctlg=010_Guys&cid=999&source=8&size=&s=
and charcoal:
http://www.tillys.com/tillys/variants.aspx?prod=169294110&ctlg=010_Guys&cid=999&s... 阅读全帖
c****x
发帖数: 1270
21
来自主题: GunsAndGears版 - USP到FFL了,交半个作业

USP Variants:
Variant 1:
 Double action/single action with "SAFE” position. Control
lever (manual safety/decoking lever) on left side of frame.
Variant 2: 
Double action/single action with "SAFE” position. Control
lever (manual safety/decocking lever) on right side of frame.
Variant 3: 
Double action/single action without "SAFE” position.
Control lever (decocking lever) on left side of frame.
Variant 4: 
Double action/single action without ”SAFE” position.
Control lever ... 阅读全帖
w*******y
发帖数: 60932
22
Tilly's is offering an additional 30% discount off sale items:
http://www.tillys.com/tillys/sale.aspx
with code: SALE30
Shipping is free TODAY ONLY
Link to sale items:
http://www.tillys.com/tillys/sale.aspx
Link to sale items:
http://www.tillys.com/tillys/sale.aspx
Link to sale items:
http://www.tillys.com/tillys/sale.aspx
Link to sale items:
http://www.tillys.com/tillys/sale.aspx
Clothing, shoes, and accessories are included - sizes are limited. Brands
include Adidas, Billabong, Converse, DC... 阅读全帖
H****g
发帖数: 14447
23
第一组数据的原始数据如下
Brazil 1975-1980 Estimate variant 61.5
Brazil 1970-1975 Estimate variant 59.5
Brazil 1950-1955 Estimate variant 50.9
China 1975-1980 Estimate variant 65.3
China 1970-1975 Estimate variant 63.2
China 1950-1955 Estimate variant 40.8
India 1975-1980 Estimate variant 53.9
India 1970-1975 Estimate variant 50.4
India 1950-1955 Estimate variant 37.9
第二组数据的原始数据:
1980 2007
China 66.0 72.9
India 55.1 63.4
Brazil 62.5 72.2
l**********1
发帖数: 5204
24
来自主题: Biology版 - NGS数据分析的流程
Plus
To LZ:
just check,
>http://bcbio.wordpress.com/tag/ngs/
cited:
>Access VCF variant information
>In addition to extending the GATK through walkers and annotations you can
also utilize the extensive API directly, taking advantage of parsers and
data structures to handle common file formats. Using Clojure’s Java
interoperability, the variantcontext module provides a high level API to
parse and extract information from VCF files. To loop through a VCF file and
print the location, reference alle... 阅读全帖

发帖数: 1
25
回徐州已经好几天了,薇茵一直住在宾馆里,因为薇茵的父亲前几天说过,如果他见到
薇茵,一定会打断薇茵的一条腿……薇茵做错了什么事情,能让她的父亲说出这种绝情
的话?
只因薇茵改变了对公公的称呼,不叫他爸了,但孩子们仍然叫他爷爷……
1
贪杯的丈夫车祸去世,我满是绝望
我和丈夫槚秋是同乡,在外打工时相爱,就在我们确定结婚日期的第二个月,槚秋和几
个同乡在外吃饭时喝多了酒,回家的路上与路人因为擦碰这点小事而大打出手,结果造
成对方重伤,脾脏摘除,赔偿之后槚秋又被判了一年。
人进了监狱,准备结婚的钱也赔偿了,亲友全都劝我和槚秋分手,说槚秋喜欢喝酒,真
要和他结婚了,将来因为喝酒,还会生出别的事来。我父母不但让我和槚秋分手,还打
算去槚秋父母那里索要赔偿,被我拦住了。我父母问我是什么打算,分还是不分。我说
不分,给槚秋一次机会,相信他回来后会记住这一次教训的,而且我也会监督他。
槚秋出来后,我们没过多久就举行了婚礼。为了给槚秋找一条挣钱的路子,公公贷款买
了辆工程车,槚秋在外到处接活。我生下一对双胞胎,留在家里带孩子,日子过得辛苦
但甜甜蜜蜜的。公公是家里的顶梁柱,对槚秋的要求也很严格,带槚秋在... 阅读全帖

发帖数: 1
26
来自主题: Biology版 - project求建议
是的。最重要的是5,如果结果这个基因knockout的确改变了疾病的phenotype,这一个
结果就可以引起领域里的关注了。但是:
第一,这个基因其实出现一段时间了,我和老板都很惊讶一直没有这个基因knockout
mice的结果报道,所以我强烈怀疑其他group也做了这个基因,但是结果不如意,所以
就一直没消息。
第二,这个noncoding variants虽然在这个基因内部,但是要知道不是说variant is
within the gene就说明这个variant一定调控这个基因本身。横跨几个megabase的long
-range调控其他基因的比比皆是。当然了,调控所在基因是所有正常人的第一反应,
是最大可能性的hypothesis
所以我个人是觉得首先要搞清楚noncoding variants到底在调控什么,所以我们去切了
大脑样本做eqtl,测量variants所在基因的表达量,但效果不太好,所以我现在对“这
个variant调控所在基因”这个结论不是非常自信和相信。而且eqtl本来就是个非常非
常noisy的实验,而且你都不知自己切的brain区域是否跟疾病有关系(... 阅读全帖

发帖数: 1
27
VTOL项目总师Kevin Renshaw; F35垂直起降技术(3BSD)来源于通用的Model 200,与
雅克141无关
F-35B Lightning II Three-Bearing Swivel Nozzle
By Kevin Renshaw Posted 12 August 2014
The Convair Model 200 was proposed in June 1972 to respond to the US Navy
request for designs for a fighter/attack aircraft for the sea control ships.
The VTOL aircraft would have used a PW401 engine with an afterburning 3BSD
plus twin Allison XJ99 lift engines located behind the cockpit for added
vertical lift forward of the center of gravity to b... 阅读全帖
b******o
发帖数: 5644
28
来自主题: GunsAndGears版 - P226?

从这个坛子来的,原贴也是位叫大口径的高手。
http://www.hk-usa.com/phpBB3/viewtopic.php?f=5&t=8646
除了扳机的磅数以外,行程也差别不少。双动/LEM很长。因为我拿它打单动,玩靶场。
所以无所谓。如果你还有别的用处,用LEM V1好像比较。重,长的扳机容易打偏,尽管
组很紧。
They do not do custom trigger jobs, but they can convert to many standard
trigger variants.
The USP/USPC as well as the HK45/C can be converted to any one of the 9
trigger variants as well as any of the 3 LEM trigger variants, 1,2, or 4.
The P30 and P2K/SK in V3 are not convertible by HK. They are what they are:
SA/DA, decock only.
T... 阅读全帖
l****h
发帖数: 28
29
http://cellularinsights.com/iphone7/
iPhone 7 Plus: A Tale of Two Personalities
Like clockwork, every September the entire tech world gets excited for the
newest Apple device. The combination of a premium build, unmatched system
performance, and tightly integrated software and services delivers what’s
considered to be the gold standard in smartphone user experience.
Over the past five years, Apple’s modem supplier was Qualcomm, but this
year, Apple has taken a different approach with the decisio... 阅读全帖
I********l
发帖数: 8702
30
在1950-1980年期间,阿尔及利亚的人均预期寿命增长了14.9岁,增幅为34.6%。
中国同期增长了24.6岁,增幅为60%。
Algeria 1975-1980 Medium variant 58.0
Algeria 1970-1975 Medium variant 54.5
Algeria 1965-1970 Medium variant 51.4
Algeria 1960-1965 Medium variant 48.3
Algeria 1955-1960 Medium variant 45.7
Algeria 1950-1955 Medium variant 43.1
数据来源:data.un.org
i*****s
发帖数: 4596
31
来自主题: Military版 - 东亚女胸小的原因找到了
http://www.nytimes.com/2013/02/15/science/studying-recent-human

East Asian Physical Traits Linked to 35,000-Year-Old Mutation
By NICHOLAS WADE
Published: February 14, 2013
Gaining a deep insight into human evolution, researchers have identified a
mutation in a critical human gene as the source of several distinctive
traits that make East Asians different from other races.
The traits — thicker hair shafts, more sweat glands, characteristically
identified teeth and smaller breasts — are the re... 阅读全帖
I********l
发帖数: 8702
32
邓产党捅鸡菊的数据又被造谣蛋当真理了?8年里把人均寿命提高22岁?出处也就是这
一处。要不请你提供邓产党捅鸡菊发布的各年份数据?如此荒谬的数据,很可能就是一
个笔误,却被你深信不疑,你说你不是弱智有人信没有啊?
最后还是看看联合国的数据吧,
China 1975-1980 Medium variant 66.3
China 1970-1975 Medium variant 64.6
China 1965-1970 Medium variant 59.4
China 1960-1965 Medium variant 44.0
China 1955-1960 Medium variant 45.0
China 1950-1955 Medium variant 44.6
I********l
发帖数: 8702
33
来自主题: Military版 - 看看国家统计局如何抽毛轮的
好蛋自己抽自己脸,所以没人理他。
发信人: Irrational (Herzog), 信区: Military
标 题: Re: 诺奖得主父亲在河南被饿死 旧居竟变爱国教育基地
发信站: BBS 未名空间站 (Sat Jun 15 13:16:16 2013, 美东)
邓产党捅鸡菊的数据又被造谣蛋当真理了?8年里把人均寿命提高22岁?出处也就是这
一处。要不请你提供邓产党捅鸡菊发布的各年份数据?如此荒谬的数据,很可能就是一
个笔误,却被你深信不疑,你说你不是弱智有人信没有啊?
最后还是看看联合国的数据吧,
China 1975-1980 Medium variant 66.3
China 1970-1975 Medium variant 64.6
China 1965-1970 Medium variant 59.4
China 1960-1965 Medium variant 44.0
China 1955-1960 Medium variant 45.0
China 1950-1955... 阅读全帖
j****i
发帖数: 68152
34
来自主题: Military版 - 在爷爷奶奶家,11岁的小朋友
大年初一(2月16日),在爷爷奶奶家,11岁的小朋友,本是吃糖领红包的年纪,却在不
经意间阻止了一场山林大火的发生。
600) this.width=600" alt="11岁男孩只身扑山火 爸爸为他点赞但也告诫先报警" src
="http://web.popo8.com/201802/22/8/303432ccd2.jpg" width="600" height="787" style="max-width: 600px; color: rgb(0, 0, 0); font-family: 宋体; font-size: 14.6667px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: ... 阅读全帖
r********n
发帖数: 7441
35
来自主题: Military2版 - Iran Missile Milestones zt
Iran Missile Milestones
http://www.iranwatch.org/wmd/wponac-missilemilestones.htm
Updated July 2011
1985: Then-speaker of the Iranian Majlis Ali Akbar Hashemi Rafsanjani leads
a high-level delegation to Libya, Syria, North Korea and China, reportedly
to acquire missiles.
1985: Iran receives its first Scud-Bs from Libya.
1987: China sells Iran "Silkworm" anti-ship cruise missiles.
1987: Iran reportedly receives approximately 100 Scud B missiles from North
Korea. Iran had allegedly agreed to finan... 阅读全帖
t**x
发帖数: 20965
36
我可以跟你说这个写这个文章的医生是一个十恶不赦的王八蛋!因为这个德国医生就是
跟西雅图儿童医院勾结的个婊子养的畜生。
这是我的信:
Von: Andrew Xie [mailto:[email protected]/* */]
Gesendet: Montag, 19. September 2016 13:24
An: Wieser, Dr. Thomas, Chefarzt
Betreff: RE: Question on a benign mutation / NM_000098.2(CPT2):c.1102G>A (p.
Val368Ile) Simple - Variation Report - ClinVar – NCBI
Dear Dr. Wieser,
Thanks for your reply.
In the link it says 3 sources reporting ‘benign’ :
1. GeneDX on May 29, 2013
2. Emory Genetics Laboratory on Apr 16, 2014... 阅读全帖
p**********u
发帖数: 15479
37
来自主题: Missouri版 - 东亚女胸小的原因找到了 (转载)
【 以下文字转载自 WaterWorld 讨论区 】
发信人: ldy (现在我很害怕,可能时间会炸), 信区: WaterWorld
标 题: 东亚女胸小的原因找到了
发信站: BBS 未名空间站 (Fri Feb 15 11:11:04 2013, 美东)
发信人: inergas (大坏猪), 信区: Military
标 题: 东亚女胸小的原因找到了
发信站: BBS 未名空间站 (Fri Feb 15 09:21:19 2013, 美东)
http://www.nytimes.com/2013/02/15/science/studying-recent-human

East Asian Physical Traits Linked to 35,000-Year-Old Mutation
By NICHOLAS WADE
Published: February 14, 2013
Gaining a deep insight into human evolution, researchers have identified a
mutation in a critic... 阅读全帖
h*********2
发帖数: 2508
38
来自主题: GunsAndGears版 - Gun Board Classic
有点长,但是挺好玩
After some years now of reading internet bulletin boards, I think I’ve got
the pros and cons of possible SHTF rifle choices figured out. Correct me if
I’m wrong, but the following is my analysis based upon the wisdom of
numerous gun board gurus (you know them, they’re always the first ones to
tell you a particular model gun is “junk” and enlighten you as to why they
have made the only logical purchases)…
The AR 15:
Great, awesome, unbelievable rifle(when it works). Can hit a fly in the... 阅读全帖
l*y
发帖数: 21010
39
来自主题: Fashion版 - 东亚女胸小的原因找到了
【 以下文字转载自 Military 讨论区 】
发信人: inergas (大坏猪), 信区: Military
标 题: 东亚女胸小的原因找到了
发信站: BBS 未名空间站 (Fri Feb 15 09:21:19 2013, 美东)
http://www.nytimes.com/2013/02/15/science/studying-recent-human

East Asian Physical Traits Linked to 35,000-Year-Old Mutation
By NICHOLAS WADE
Published: February 14, 2013
Gaining a deep insight into human evolution, researchers have identified a
mutation in a critical human gene as the source of several distinctive
traits that make East Asians different from other races.... 阅读全帖
l*y
发帖数: 21010
40
来自主题: WaterWorld版 - 东亚女胸小的原因找到了
【 以下文字转载自 Military 讨论区 】
发信人: inergas (大坏猪), 信区: Military
标 题: 东亚女胸小的原因找到了
发信站: BBS 未名空间站 (Fri Feb 15 09:21:19 2013, 美东)
http://www.nytimes.com/2013/02/15/science/studying-recent-human

East Asian Physical Traits Linked to 35,000-Year-Old Mutation
By NICHOLAS WADE
Published: February 14, 2013
Gaining a deep insight into human evolution, researchers have identified a
mutation in a critical human gene as the source of several distinctive
traits that make East Asians different from other races.... 阅读全帖
u*********1
发帖数: 2518
41
来自主题: Biology版 - 求教生物信息学问题
既然你有了全基因组的序列,就赶紧在这些SNP上下功夫;先不说association的问题,
如果你的hypothesis是rare/novel variants导致这个phenotype(也就是上面说的
effect size很大),那么你的1500个SNPs完全可以那去和数据库的common variants做
比对,找出罕见的同时改变protein序列的missense mutation或者影响splicing的,甚
至miRNA target啥的,也就是annotation。我不是做线虫的,但我估计也肯定是有相关
的database的吧。这一步大家一般用annovar这个软件
如果你的phenotype是个罕见的missense mutation导致的,那么你的9个样本中哪怕出
现了2个都是很有意义的。当然我的这套办法都是对于human genome的,如果你寻找的
是common variants或者更复杂的epistasis啥的就当我上面的没说。
association study往往是对于comman variants的,最经典的就是用plink。不过plink
也是好复杂... 阅读全帖

发帖数: 1
42
来自主题: Biology版 - project求建议
小弟做某神经疾病关联位点的分子机理,已经做了的东西有:
1.首次发现这个相关位点,这个variants在某个基因的intron区域 (所以难研究诶)
2.把这个variants克隆进luciferase系统研究对表达的影响,有明显影响
3.我们有疾病相关的病人大脑组织样本,直接拿来进行eQTL的研究,但病人样本太
noisy所以结果不够significant,但trend和luciferase系统是一致的
4. 因为大脑样本结果不够明显,我也在用neuroblastoma的cell line做crispr,正在
等待结果
5. 同时合作者弄到了variants所在基因的knockout mice,正在等待结果看这个基因的
敲除是否对老鼠有影响
同时可能打算要做的:
6. 我通过public data计算发现这个variants所在区域可能binding的protein,所以打
算做chip-seq?
7. 最终极的还是直接在ips/stem cell里做crispr产生这个variants,然后诱导成我们
要的neuron。但是我对ips技术实在不懂又要从头学起。
老板是MD,所以思维... 阅读全帖
l**i
发帖数: 8144
43
来自主题: Military版 - Anthropic principle
简单地讲 就是谋事在人 成事在天
认为社会发展沿着某个预定方向发展的理论 都是傻逼理论
--------------------------------------
Anthropic principle
From Wikipedia, the free encyclopedia
In astrophysics and cosmology, the anthropic principle is the philosophical
argument that observations of the physical Universe must be compatible with
the conscious life that observes it. Some proponents of the argument reason
that it explains why the Universe has the age and the fundamental physical
constants necessary to accommodate conscious life. As a ... 阅读全帖
B*********e
发帖数: 254
44
来自主题: TrustInJesus版 - The Johannine Comma
Bible Research > Textual Criticism > Johannine Comma
The Johannine Comma
(1 John 5:7-8)
The so-called Johannine Comma (also called the Comma Johanneum) is a sequenc
e of extra words which appear in 1 John 5:7-8 in some early printed editions
of the Greek New Testament. In these editions the verses appear thus (we pu
t backets around the extra words):
?τι τρε?? ε?σιν ο? μαρτυρο?ντε? [?ν τ? ο?ραν?, ? Π
ατ?ρ, ? Λ?γο?, κα? τ? ?γιον Πνε?μα? κα? ο?τοι ο? τ
ρε?? ?ν ε?σι. 8 κα? τρε?? ε?σιν ο? μαρτυρο?ντ... 阅读全帖
k*****d
发帖数: 12
45
参观回来汇报一下。
公司不大,一起去的同事数了一下椅子,有30把左右,还有空着的办公桌和椅子。
没有wet lab,一间大办公室加几个小会议室。
可以把你的DNA sample给他们,他们给你找测序公司去测序,我的印象是送到Illumina
还有BGI或者类似的地方。
Alignment和call variants这些步骤就是用open source的软件,比如BWA和GATK。他们
的主要工作是用他们的数据库帮你annotate你的sample的variants,比如是不是已知的
somatic mutation或某个GWAS study里发现的相关SNP,这部分叫knome variant。这个
数据库包括已经报道的variants, 比如cosmic,还有一些文献报道的数据,但他们没有
力量跟踪所有的pubmed的文章。这个数据库和它的客户端的程序都可以卖给你,数据库
是SQLite,就放在local hard disk上,你也可以自己写SQL statement。还有一部分叫
knome pathway,帮助分析你的那些variants有没有集中在某些pathway里。
l**********1
发帖数: 5204
46
来自主题: Biology版 - tools to open GFF3 or GVF files
GVF2VCF
GVF to VCF file converter (currently beta)
The Genome Variation Format (GVF) is a file format for describing sequence
variants at nucleotide resolution relative to a reference genome. GVF dumps
are available from the Ensembl FTP site. However, many tools prefer to use
the 1000 Genomes VCF format (e.g. Genome Analysis Toolkit), so a converter
is often needed. This converter currently only supports output in VCF v4.0.
Large, inexact structural variants will therefore be skipped (v4.1 of VC... 阅读全帖
T**7
发帖数: 264
47
来自主题: Biology版 - SPSS数据如何输入?
输入格式很像excel的格式
我有两组数字,比较显著性差异
比如,一组是5,7,6,8,9
另一组是11,13,25,19,20
请问如何输入?
横向是vairant 1,variant 2, variant 3。。。。。
纵向是1,2,3,4,5,6,。。。。
我把两组数字一组列在variant 1下方,纵向排列
另一组数字在variant 2下方,纵向排列
发现无法分析,means-anova-viriant 1, variant 2都add
请问如何输入两组数字呢?
谢谢了!
l******u
发帖数: 936
48
http://investor.illumina.com/phoenix.zhtml?c=121127&p=irol-news
Illumina’s MiSeqDxTM Receives FDA Premarket Clearance with Two Cystic
Fibrosis Assays and Universal Kit for Open Use
Premarket Clearance is an Industry First for a Next-Generation Sequencing
System
SAN DIEGO--(BUSINESS WIRE)--Nov. 19, 2013-- Illumina, Inc. (NASDAQ:ILMN)
today announced that it received premarket clearance from the U.S. Food and
Drug Administration (FDA) for the MiSeqDx system, the first high-throughput
DNA sequencin... 阅读全帖
c**i
发帖数: 6973
49
来自主题: Military版 - More on New Arms Package
Wendell Minnick, Taiwan Sends Mixed Message on Fighters. Defense News, May
14, 2010.
http://www.defensenews.com/story.php?i=4626494&c=ASI&s=AIR
Note:
(a) F-35 Lightning II
http://en.wikipedia.org/wiki/F-35_Lightning_II
F-35 has three variants:
"F-35A, conventional take off and landing (CTOL) variant.
F-35B, short-take off and vertical-landing (STOVL) variant.
F-35C, carrier-based CATOBAR (CV) variant.
(b) AV-8B Harrier II
http://en.wikipedia.org/wiki/AV-8B_Harrier_II
(in use since 1985)
* Harrie

发帖数: 1
50
来自主题: Military版 - 刘强东太搞笑了
明尼苏达州明尼亚波利斯警察局24日公布京东创始人兼首席执行官刘强东,涉嫌强暴明
尼苏达大学中国女留学生刘静尧( Jingyao Liu)案的所有证据及长达149页的调查报告
,其中包括在女方租住公寓楼、餐馆、明大商学院内录得的高清版监视影片,以及之前
未曝光的警方访问刘强东录音,刘强东在访问中表示女方主动邀请他到公寓,还帮他洗
澡,两人性交后均熟睡了好几个小时;而警方抵达后,女学生曾告诉他"这是一个可怕
的误会"。
明州警局向本报表示,这些影片、报案录音、警察报告均已依照法律规定,进行了
必要的剪辑审查,所有内容包括刘静尧男性友人8月30日拨打911报案的电话录音文字档
,刘强东去年9月10日接受警方问话的电话录音,刘强东进入女方公寓及在明大商学院
内遭逮捕的照片,女学生与当时人在中国的好友、男友等人的短讯对话,案发日餐馆、
公寓的高清版监视影片,以及警方的全案报告。
根据警方公佈去年9月10日的访问刘强东的约30分钟录音档显示(见文章后面的附
件),8月30日餐会中女方只喝了一点酒,之后在搭他的车子到预计展开第二摊聚会时
,女方主动邀请他去其新搬入的公寓看看,并给了司机地址,接着就到... 阅读全帖
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