l*********1
发帖数: 351 | 1 structural diversity不如mAb问题不大。关键的是molecular weight不合适，再怎么
提高affinity，肿瘤部位的聚集效果不如mAb。直接的造成的问题就是biodistribution
比较烂，造成off-target effect，心脏肾脏尤为明显。Wittrup有两篇相关的文章，理
论上推到出这个结论。Darpin的实验结果也符合Wittrup的模型。所以现在Darpin也开
始做PEGylation了。。。 |
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l***d
发帖数: 1798 | 2 FOR IMMEDIATE RELEASE
National Academy of Engineering Elects 66 Members and 10 Foreign Associates
WASHINGTON — The National Academy of Engineering (NAE) has elected 66 new m
embers and 10 foreign associates, announced NAE President Charles M. Vest to
day. This brings the total U.S. membership to 2,254 and the number of forei
gn associates to 206.
Election to the National Academy of Engineering is among the highest profess
ional distinctions accorded to an engineer. Academy membership honors th... 阅读全帖 |
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w******e
发帖数: 1187 | 3 让我对droplet/IVC的饭度大减呵呵。跟phage比也有definitive的优势——
毕竟是HT screening而不是selection。我小见识浅薄一惊一乍大家别见怪呵呵。
ms就wittrup一枝独秀？国内有phage/yeast display的牛人吗？
内行指点一下？ |
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w******e
发帖数: 1187 | 4 I remember you are the expert on this. got a question for you: is yeast
display officially a better method for affinity maturation now?
some work from Wittrup group is amazing...
its
significantly
being FACSable is a big deal to me-- quantitative, monitoring individual
clone
that's a problem for every display I guess, or do you mean it's a much
bigger concern for yeast? |
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H****s
发帖数: 301 | 5 Not an expert on this topic, but I am willing to share my 2 cents.
For antibody affinity maturation, yeast display is better than other
technologies. FACS sorting, combined with yeast display, increases the
throughput significantly. You can quantify binding affinity and kinetics
with flow cytometry. At the same time, you do not need to work with a
library larger than 10e10, which is still a pain in the field. Dr. Wittrup
established a company in New Hampshire. The name is Adimab. You can check
t... 阅读全帖 |
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w******e
发帖数: 1187 | 6 well, try Wittrup next:) |
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q***l
发帖数: 177 | 7 National Academy of Engineering Elects 66 Members and 10 Foreign Associates
WASHINGTON — The National Academy of Engineering (NAE) has elected 66 new
members and 10 foreign associates, announced NAE President Charles M. Vest
today. This brings the total U.S. membership to 2,254 and the number of
foreign associates to 206.
Election to the National Academy of Engineering is among the highest
professional distinctions accorded to an engineer. Academy membership
honors those who have made outstand... 阅读全帖 |
|
g******6
发帖数: 782 | 8 http://www.nae.edu/56154.aspx
National Academy of Engineering Elects 66 Members and 10 Foreign Associates
WASHINGTON — The National Academy of Engineering (NAE) has elected 66 new
members and 10 foreign associates, announced NAE President Charles M. Vest
today. This brings the total U.S. membership to 2,254 and the number of
foreign associates to 206.
Election to the National Academy of Engineering is among the highest
professional distinctions accorded to an engineer. Academy membership
honor... 阅读全帖 |
|
