R*s
发帖数: 2041 | 1 for stable transfection: u include a selective marker (such as G418,
puromycin) in the DNA construct will transfect cells with. Then by
including the antibiotics (G18, puro...) in the media, you will be
able to seletively grow up the cells trasfected with your construct, while
killing the untrasfected ones.
For transient transfection: you don't have such selective markers in
the constracuts. You will always grow cells in regular media. Since it
is impossible to achieve 100% transfection, there i |
|
