r****o
发帖数: 105 | 1 I have similar experiences as yours. That is about determination
the anomericity of glycosylation on EGF repeats. It involves
processing the small domain EGF into peptides, and then subject them
to glucosidase digestion.
I would suggest you using HPLC with either Reverse Phase or Gel filtration
column(like superdex from Pharamacia). This is the best way to recover
small amount of peptides. If your sample has quite a lot and the MW is
big enough, you can try acetone precitation to get rid of |
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