f***r
发帖数: 19 | 1 1) Do your realtime primer/probe span exons? If not, genome contamination may
cause the discrepancy.
2) Narrow down your standard curve range, for example using a two or three
times dilution intead of ten.
3) It seems you use absolute standard curve method. If you just wanna compare
two samples, why not use total cDNA make standard curve and normalize to
housekeeping gene? |
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