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发帖数: 3 | 1 That's why they do normalization. Actually there is always variability between
two microarrays. Even you use same amount of RNA from the same biological
sample, preparation and hybridization will definitely bring in bias. Because
there are too many steps involved and it's impossible to make them same
between any two arrays: reverse transcription, labeling, hybridization,
washing, scanning......So they use housekeeping control to normalize sample
preparations and spike-in control to normalize hyb |
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