m*****i
发帖数: 29 | 1 Check out this paper:
Xu ZL, Mizuguchi H, Mayumi T, Hayakawa T.
Regulated gene expression from adenovirus vectors: a systematic comparison of
various inducible systems.
Gene. 2003 May 8;309(2):145-51.
Actally I wasted half year on Clonetech's tet-on/off system. It is very leaky
so that in some cell types (MEF is worst) you can never control the basal mRNA
expression level at all. After half year's work, I called the campany and also
found out this paper that compared different commercial inducib |
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