t*x
发帖数: 1065 | 1 I do mid-brain cultures from mouse embryos. So far, my cells are not
consistent. Sometimes they spread out individually (great), but sometimes
they clump up as clusters. It looks like a coating problem. I coated with
poly-D-lysine at 0.1mg/ml overnight and washed with water twice and then
10ug/ml laminin coating overnight. After that, wash by water twice and dried.
Anyone has suggestion about a coating technique that is consistent with cell
spreading (not clumping)?
Thanks a lot! | p*******r
发帖数: 4048 | 2 Wash your glass well.
sometimes
with
dried.
cell
【在 t*x 的大作中提到】
: I do mid-brain cultures from mouse embryos. So far, my cells are not
: consistent. Sometimes they spread out individually (great), but sometimes
: they clump up as clusters. It looks like a coating problem. I coated with
: poly-D-lysine at 0.1mg/ml overnight and washed with water twice and then
: 10ug/ml laminin coating overnight. After that, wash by water twice and dried.
: Anyone has suggestion about a coating technique that is consistent with cell
: spreading (not clumping)?
: Thanks a lot!
| a****d
发帖数: 1919 | 3 I do one hour coating and H2O washing 3X times. You may also try PLL,
laminin, etc... |
|
