c*t
发帖数: 1063 | 1 请教前辈：
用cre和loxp的老鼠得到最终的KO mice之后，是不是可以用产生的其他double
heterozygotes （A-cre;Bflox/+ X A-cre;Bflox/+)来交配产生KO mice？就不用
follow原来的breeding strategy了吧？ |
d***3
发帖数: 181 | 2 Will the yield be too low? |
H****N
发帖数: 997 | 3 Yes, 3/16 will be cKO, but some of them (1/3) have two cre alleles, and some
of them (2/3) have only one. |
w****8
发帖数: 36 | 4 The best strategy is cross Cre; fl/fl mice with fl/fl mice. then you will
get almost half-half.
Good luck. |
w*e
发帖数: 740 | 5 A-CRE在生殖细胞表达么？
如果是，B基因的缺失会不会影响生育？
【在 w****8 的大作中提到】
: The best strategy is cross Cre; fl/fl mice with fl/fl mice. then you will
: get almost half-half.
: Good luck.
|
s*********e
发帖数: 399 | 6 http://physiolgenomics.physiology.org/cgi/content/abstract/35/1/1
read this paper before you go to next step.
CRE may go to germline and your cell type specificity may be lost . |
c*t
发帖数: 1063 | 7 谢谢大家的回答。
是不是 A gene不在生殖细胞表达，就不会出现此文章中的情况了？
算了下原来的breeding strategy，得到cKO的几率是3/8,如果用double heterozygotes
会降到3/16。
Thanks. |
w****8
发帖数: 36 | 8 i am using actin-CreER line so that i don't need to think about the germline
issue. Just be careful and try. Good luck. |
w****8
发帖数: 36 | 9 and never cross the mice both have the cre allel, because then you cannot
distinguish the homo and hetero of
the cre line.
【在 c*t 的大作中提到】
: 请教前辈：
: 用cre和loxp的老鼠得到最终的KO mice之后，是不是可以用产生的其他double
: heterozygotes （A-cre;Bflox/+ X A-cre;Bflox/+)来交配产生KO mice？就不用
: follow原来的breeding strategy了吧？
|
n********k
发帖数: 2818 | 10 well, u'd better establish that Cre doesn't pose toxicity in your system
first...it seems trivial but I think many
studies in the literature could be resulted from Cre-phenotype:)))...I
personally prefer Cre/Cre F/+ versa F/+
breeding...that way u can everything...and u could unexpectedly notice hez
phenotype too...And I see I might
kill a MS if they don't show this cre control esp when the phenotype is
subtle and cell death related...
【在 w****8 的大作中提到】
: The best strategy is cross Cre; fl/fl mice with fl/fl mice. then you will
: get almost half-half.
: Good luck.
|
