d******r
发帖数: 124 | 1 ambiom, invitrogen or biorad?
用过的xdjms比较下吧。谢过！ | T****r
发帖数: 4006 | 2 ambion没用过
invitrogen，bio-rad都用过，印象中都还还不错 | d******r
发帖数: 124 | 3 非常谢谢
再问一句：一般用什么做internal control？ actin？GAPDH？ 还是S18 rRNA？
【在 T****r 的大作中提到】
: ambion没用过
: invitrogen，bio-rad都用过，印象中都还还不错
| A******y
发帖数: 2041 | 4 If you are using one-step, Quanta Biosciences kits are the best. I have
used all major brands (new Bio-rad, Applied Bio, Invitrogen, etc). Quanta
is the OEM for Bio-rad for long time. You also want to make sure that you
have two housekeeping genes and normalize your mRNA (cDNA) loads as a third
control. I like GAPDH and S18 rRNA. |
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