t****p
发帖数: 1504 | 1 For example, if you randomly put insert into genome and cover/saturate all
of genes, therefore create a large mutant strains library. How to find these
essential genes?
If there is no any insert in a gene coding region from this mutant library,
we would say this is an essential gene. But how about all of insertions
happen at very c-terminal of the coding region? Would you still call this
essential gene? What is the standard? |
a***e
发帖数: 1010 | 2 all those standards are arbitrary somehow. |
a****o
发帖数: 1786 | 3 今天听Gerald Fink的报告
5% of essential genes in one background of s. cerevisiae is not essential in
S288C background and they are called conditional essentials.
【在 a***e 的大作中提到】
: all those standards are arbitrary somehow.
|
a***e
发帖数: 1010 | 4 and if you change a growth condition, that number will change again.
essential in
【在 a****o 的大作中提到】
: 今天听Gerald Fink的报告
: 5% of essential genes in one background of s. cerevisiae is not essential in
: S288C background and they are called conditional essentials.
|
g****0
发帖数: 425 | 5 which is well demonstrated in mouse genetics as well.
【在 a***e 的大作中提到】
: and if you change a growth condition, that number will change again.
:
: essential in
|
