t****p 发帖数: 1504 | 1 For example, if you randomly put insert into genome and cover/saturate all
of genes, therefore create a large mutant strains library. How to find these
essential genes?
If there is no any insert in a gene coding region from this mutant library,
we would say this is an essential gene. But how about all of insertions
happen at very c-terminal of the coding region? Would you still call this
essential gene? What is the standard? |
a***e 发帖数: 1010 | 2 all those standards are arbitrary somehow. |
a****o 发帖数: 1786 | 3 今天听Gerald Fink的报告
5% of essential genes in one background of s. cerevisiae is not essential in
S288C background and they are called conditional essentials.
【在 a***e 的大作中提到】![](/moin_static193/solenoid/img/up.png) : all those standards are arbitrary somehow.
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a***e 发帖数: 1010 | 4 and if you change a growth condition, that number will change again.
essential in
【在 a****o 的大作中提到】![](/moin_static193/solenoid/img/up.png) : 今天听Gerald Fink的报告 : 5% of essential genes in one background of s. cerevisiae is not essential in : S288C background and they are called conditional essentials.
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g****0 发帖数: 425 | 5 which is well demonstrated in mouse genetics as well.
【在 a***e 的大作中提到】![](/moin_static193/solenoid/img/up.png) : and if you change a growth condition, that number will change again. : : essential in
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