g***y
发帖数: 201 | 3 yes, you can.
Basically,
1. snap-freeze the tissue (presumably unfixed) in Tissue-Tek by liquid
nitrogen.
2. cut sections(I prefer 5-7um). It seems the thinner, the better the
sections stay on the slide. We use plus slide from fisher
3. air-dry at room temperature for 3-5 hours.
4. 2xPBS 3min each to clear tissue-tek
5. Postfix in 2% to 4% PFA in 1xPBS for 5-10 min
6. Wash away PFA by 3xPBS 3 min each
7.PBS+Triton x-100 0.05% 5 min(optional)
If you are going to do immunofluorescence, you can go
【在 s*****t 的大作中提到】
: 有谁做过？可不可以发一份ｐｒｏｔｏｃｏｌ到我email：J**[email protected]
: 5个包子的奖励，
|
